Restriction map for
pFN21AE4560
(Circular) (Six-base) MAPSORT of: pFN21AE4560.seq Check: 7531 from: 1 to: 6760
pFN21AE4560 6760 bp
With 182 enzymes: SgfI *
March 28, 2012 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3055 3055
Size: 6760
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4570 278
Size: 53 83 186 936 3034 2468
Fragments arranged by size:
3034 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3673 4049 4103 1937
Size: 1736 376 54 4594
Fragments arranged by size:
4594 1736 376 54
Acc65I G'GTAC_C
Cuts at: 4081 4081
Size: 6760
AclI AA'CG_TT
Cuts at: 1563 4887 5260 1563
Size: 3324 373 3063
Fragments arranged by size:
3324 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2031 2156 3174 3585 4823 6207 6475 1632
Size: 399 125 1018 411 1238 1384 268 1917
Fragments arranged by size:
1917 1384 1238 1018 411 399 268 125
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6563
AflIII A'CryG_T
Cuts at: 1203 5659 6636 1203
Size: 4456 977 1327
Fragments arranged by size:
4456 1327 977
AgeI A'CCGG_T
Cuts at: 5591 5591
Size: 6760
AhdI GACnn_n'nnGTC
Cuts at: 5489 5489
Size: 6760
AleI CACnn'nnGTG
Cuts at: 1166 2652 3065 1166
Size: 1486 413 4861
Fragments arranged by size:
4861 1486 413
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6760
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2207 2239 2207
Size: 32 6728
AlwNI CAG_nnn'CTG
Cuts at: 3708 4503 6075 3708
Size: 795 1572 4393
Fragments arranged by size:
4393 1572 795
ApaI G_GGCC'C
Cuts at: 2370 2370
Size: 6760
ApaLI G'TGCA_C
Cuts at: 4817 5973 4817
Size: 1156 5604
ApoI r'AATT_y
Cuts at: 1493 2860 2898 2954 3796 4069 4295 4447 6556
Size: 1367 38 56 842 273 226 152 2109
Cuts at: 6556 1493
Size: 1697
Fragments arranged by size:
2109 1697 1367 842 273 226 152 56
38
AseI AT'TA_AT
Cuts at: 160 1032 2624 3504 5314 160
Size: 872 1592 880 1810 1606
Fragments arranged by size:
1810 1606 1592 880 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6760
AvaI C'yCGr_G
Cuts at: 1870 1943 2259 4085 1870
Size: 73 316 1826 4545
Fragments arranged by size:
4545 1826 316 73
BamHI G'GATC_C
Cuts at: 2110 4090 4457 2110
Size: 1980 367 4413
Fragments arranged by size:
4413 1980 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4081 5536 618
Size: 325 217 627 2294 1455 1842
Fragments arranged by size:
2294 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2175 2370 3129 4079 1391
Size: 784 195 759 950 4072
Fragments arranged by size:
4072 950 784 759 195
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2094 928
Size: 971 195 5594
Fragments arranged by size:
5594 971 195
BbvCI CC'TCA_GC
Cuts at: 3242 3242
Size: 6760
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4951 4985 1903
Size: 34 3014 34 3678
Fragments arranged by size:
3678 3014 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4652 5862 4652
Size: 1210 5550
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6760
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5371 136
Size: 107 122 71 631 605 3699 1525
Fragments arranged by size:
3699 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2379 2425 6755 2379
Size: 46 4330 2384
Fragments arranged by size:
4330 2384 46
BlpI GC'TnA_GC
Cuts at: 4177 4177
Size: 6760
Bme1580I G_kGCm'C
Cuts at: 1790 2370 4821 5977 1790
Size: 580 2451 1156 2573
Fragments arranged by size:
2573 2451 1156 580
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6760
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4491 5449 442
Size: 416 53 83 399 24 3074 958 1753
Fragments arranged by size:
3074 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6760
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3956 3956
Size: 6760
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2773 5420 1239
Size: 878 656 2647 2579
Fragments arranged by size:
2647 2579 878 656
Bpu10I CC'TnA_GC
Cuts at: 3242 3242
Size: 6760
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4885 5750 6048 4885
Size: 865 298 5597
Fragments arranged by size:
5597 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5423 882
Size: 617 3924 2219
Fragments arranged by size:
3924 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4551 493
Size: 711 3347 2702
Fragments arranged by size:
3347 2702 711
BsaBI GATnn'nnATC
Cuts at: 6754 6754
Size: 6760
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2187 4567 4949 5584
Size: 53 83 186 936 654 2380 382 635
Cuts at: 5584 275
Size: 1451
Fragments arranged by size:
2380 1451 936 654 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5193 5591 5865 6012 6423 5193
Size: 398 274 147 411 5530
Fragments arranged by size:
5530 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2207 2237 1178
Size: 30 165 30 804 30 5701
Fragments arranged by size:
5701 804 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3686 1186
Size: 2500 4260
BseYI C'CCAG_C
Cuts at: 5963 6459 5963
Size: 496 6264
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6187
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4210 4971 5999 1540
Size: 275 186 2209 761 1028 2301
Fragments arranged by size:
2301 2209 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2304 4821 4906 5977 2304
Size: 2517 85 1071 3087
Fragments arranged by size:
3087 2517 1071 85
BsmI GAATG_Cn'
Cuts at: 3078 4271 4364 3078
Size: 1193 93 5474
Fragments arranged by size:
5474 1193 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 2304 2370 3129 4079 4821 4906
Size: 399 385 129 66 759 950 742 85
Cuts at: 4906 5977 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 950 759 742 399 385 129
85 66
BspEI T'CCGG_A
Cuts at: 6423 6423
Size: 6760
BspHI T'CATG_A
Cuts at: 4649 6379 4649
Size: 1730 5030
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3055 4115 844
Size: 2211 1060 3489
Fragments arranged by size:
3489 2211 1060
BsrBI CCG'CTC
Cuts at: 1394 1823 4647 1394
Size: 429 2824 3507
Fragments arranged by size:
3507 2824 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2325 5255 5429 65
Size: 1183 1077 2930 174 1396
Fragments arranged by size:
2930 1396 1183 1077 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5404 5591 1700
Size: 110 3594 187 2869
Fragments arranged by size:
3594 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3747 96
Size: 3651 3109
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6760
BssSI C'ACGA_G
Cuts at: 2650 4054 4820 5832 2650
Size: 1404 766 1012 3578
Fragments arranged by size:
3578 1404 1012 766
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6760
BstBI TT'CG_AA
Cuts at: 5564 5564
Size: 6760
BstEII G'GTnAC_C
Cuts at: 1184 3582 1184
Size: 2398 4362
BstXI CCAn_nnnn'nTGG
Cuts at: 2234 2741 3067 3534 2234
Size: 507 326 467 5460
Fragments arranged by size:
5460 507 467 326
BstYI r'GATC_y
Cuts at: 1972 2110 2265 2379 2425 2988 4090 4457 4842
Size: 138 155 114 46 563 1102 367 385
Cuts at: 4842 4859 6311 6397 6409 6755 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1102 563 385 367 346 155
138 114 86 46 17 12
BstZ17I GTA'TAC
Cuts at: 4050 4050
Size: 6760
BtgI C'CryG_G
Cuts at: 513 1065 2004 3105 513
Size: 552 939 1101 4168
Fragments arranged by size:
4168 1101 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2290 3959 487
Size: 683 56 1064 1669 3288
Fragments arranged by size:
3288 1669 1064 683 56
BtsI GCAGTG_nn'
Cuts at: 3539 4276 5070 5090 3539
Size: 737 794 20 5209
Fragments arranged by size:
5209 794 737 20
ClaI AT'CG_AT
Cuts at: 1554 2631 1554
Size: 1077 5683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6725
DraI TTT'AAA
Cuts at: 2469 2619 2859 4064 2469
Size: 150 240 1205 5165
Fragments arranged by size:
5165 1205 240 150
DraIII CAC_nnn'GTG
Cuts at: 3610 3610
Size: 6760
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5767 809
Size: 507 4451 1802
Fragments arranged by size:
4451 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2236 2310 4207
Size: 54 1425 50 275 18 406 74 1897
Cuts at: 4207 5096 6725 8
Size: 889 1629 43
Fragments arranged by size:
1897 1629 1425 889 406 275 74 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4207 1537
Size: 275 2395 4090
Fragments arranged by size:
4090 2395 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2276 4690 1353
Size: 923 2414 3423
Fragments arranged by size:
3423 2414 923
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5347 5721 5867 387
Size: 1064 138 3758 374 146 1280
Fragments arranged by size:
3758 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 2117 2156 2773 3174 3585 4823
Size: 393 399 86 39 617 401 411 1238
Cuts at: 4823 5420 6207 6475 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1238 787 617 597 411 401 399
393 268 86 39
EcoO109I rG'GnC_Cy
Cuts at: 2366 2366
Size: 6760
EcoRI G'AATT_C
Cuts at: 4069 4447 4069
Size: 378 6382
FspI TGC'GCA
Cuts at: 5266 5266
Size: 6760
HaeII r_GCGC'y
Cuts at: 1678 5907 1678
Size: 4229 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2019 2051 2095 2127 2973
Size: 32 135 32 447 32 44 32 846
Cuts at: 2973 3005 5401 5433 5475 5507 1373
Size: 32 2396 32 42 32 2626
Fragments arranged by size:
2626 2396 846 447 135 44 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3391 4104 4350 669
Size: 988 281 1453 713 246 3079
Fragments arranged by size:
3079 1453 988 713 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6760
HpaI GTT'AAC
Cuts at: 4350 4350
Size: 6760
KpnI G_GTAC'C
Cuts at: 4085 4085
Size: 6760
MfeI C'AATT_G
Cuts at: 2675 4359 2675
Size: 1684 5076
MluI A'CGCG_T
Cuts at: 6636 6636
Size: 6760
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2663 5874 6058 1305
Size: 1358 3211 184 2007
Fragments arranged by size:
3211 2007 1358 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2238 10
Size: 54 1425 343 406 4532
Fragments arranged by size:
4532 1425 406 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 2652 2739 3065 3684 4718 5077
Size: 648 873 613 87 326 619 1034 359
Cuts at: 5077 5236 518
Size: 159 2042
Fragments arranged by size:
2042 1034 873 648 619 613 359 326
159 87
MspA1I CmG'CkG
Cuts at: 1606 4176 4853 6001 6246 1606
Size: 2570 677 1148 245 2120
Fragments arranged by size:
2570 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3105 513
Size: 552 939 1101 4168
Fragments arranged by size:
4168 1101 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6760
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6760
NotI GC'GGCC_GC
Cuts at: 4207 4207
Size: 6760
NspI r_CATG'y
Cuts at: 1132 1229 3250 3310 3406 4023 4118 5663 1132
Size: 97 2021 60 96 617 95 1545 2229
Fragments arranged by size:
2229 2021 1545 617 97 96 95 60
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6760
PciI A'CATG_T
Cuts at: 5659 5659
Size: 6760
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2766 1108
Size: 1658 5102
PfoI T'CCnGG_A
Cuts at: 2544 3012 3293 2544
Size: 468 281 6011
Fragments arranged by size:
6011 468 281
PmeI GTTT'AAAC
Cuts at: 4064 4064
Size: 6760
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2207 2239 4829 4861 6342 6374 2207
Size: 32 2590 32 1481 32 2593
Fragments arranged by size:
2593 2590 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6760
PsiI TTA'TAA
Cuts at: 2502 4330 2502
Size: 1828 4932
PspOMI G'GGCC_C
Cuts at: 2366 2366
Size: 6760
PstI C_TGCA'G
Cuts at: 830 4112 830
Size: 3282 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6760
SalI G'TCGA_C
Cuts at: 1936 4102 1936
Size: 2166 4594
SbfI CC_TGCA'GG
Cuts at: 4112 4112
Size: 6760
ScaI AGT'ACT
Cuts at: 5008 6720 5008
Size: 1712 5048
SexAI A'CCwGG_T
Cuts at: 3578 3578
Size: 6760
SfcI C'TryA_G
Cuts at: 826 1045 4108 5243 5924 6115 826
Size: 219 3063 1135 681 191 1471
Fragments arranged by size:
3063 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6760
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6760
SmaI CCC'GGG
Cuts at: 1872 4087 1872
Size: 2215 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4864 5765 6027 820
Size: 197 926 2921 901 262 1553
Fragments arranged by size:
2921 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4551 493
Size: 4058 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6760
SphI G_CATG'C
Cuts at: 1132 3250 4023 4118 1132
Size: 2118 773 95 3774
Fragments arranged by size:
3774 2118 773 95
SspI AAT'ATT
Cuts at: 5 52 3469 4684 5
Size: 47 3417 1215 2081
Fragments arranged by size:
3417 2081 1215 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6760
StyI C'CwwG_G
Cuts at: 513 1065 2004 2158 2211 2387 3105 4199 513
Size: 552 939 154 53 176 718 1094 3074
Fragments arranged by size:
3074 1094 939 718 552 176 154 53
SwaI ATTT'AAAT
Cuts at: 2859 2859
Size: 6760
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2773 4787 4804 4957 5142 5587 325
Size: 1255 1193 2014 17 153 185 445 1498
Fragments arranged by size:
2014 1498 1255 1193 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3189 3747 4033
Size: 274 80 33 51 1443 1212 558 286
Cuts at: 4033 5006 6718 96
Size: 973 1712 138
Fragments arranged by size:
1712 1443 1212 973 558 286 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3325 3357 6720 6752 3325
Size: 32 3363 32 3333
Fragments arranged by size:
3363 3333 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3020 3083 1316
Size: 1704 63 4993
Fragments arranged by size:
4993 1704 63
XbaI T'CTAG_A
Cuts at: 4096 4096
Size: 6760
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2763 3064 3318 2763
Size: 301 254 6205
Fragments arranged by size:
6205 301 254
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6760
XmaI C'CCGG_G
Cuts at: 1870 4085 1870
Size: 2215 4545
XmnI GAAnn'nnTTC
Cuts at: 2091 4889 2091
Size: 2798 3962
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4568 276
Size: 53 83 186 936 3034 2468
Fragments arranged by size:
3034 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI