Restriction map for
pFN21AE4558
(Circular) (Six-base) MAPSORT of: pFN21AE4558.seq Check: 629 from: 1 to: 6754
pFN21AE4558 6754 bp
With 182 enzymes: SgfI *
March 28, 2012 14:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4564 278
Size: 53 83 186 936 3028 2468
Fragments arranged by size:
3028 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4097 1937
Size: 2160 4594
Acc65I G'GTAC_C
Cuts at: 4075 4075
Size: 6754
AclI AA'CG_TT
Cuts at: 1563 4881 5254 1563
Size: 3318 373 3063
Fragments arranged by size:
3318 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2642 2681 2719 3264 3539 3629 4817 6201
Size: 1010 39 38 545 275 90 1188 1384
Cuts at: 6201 6469 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1188 1010 545 275 268 90
39 38
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6557
AflIII A'CryG_T
Cuts at: 1203 2463 3501 5653 6630 1203
Size: 1260 1038 2152 977 1327
Fragments arranged by size:
2152 1327 1260 1038 977
AgeI A'CCGG_T
Cuts at: 5585 5585
Size: 6754
AhdI GACnn_n'nnGTC
Cuts at: 3390 5483 3390
Size: 2093 4661
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6754
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6754
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3747 3779 3747
Size: 32 6722
AlwNI CAG_nnn'CTG
Cuts at: 4497 6069 4497
Size: 1572 5182
ApaI G_GGCC'C
Cuts at: 2280 2280
Size: 6754
ApaLI G'TGCA_C
Cuts at: 4811 5967 4811
Size: 1156 5598
ApoI r'AATT_y
Cuts at: 1493 3149 3714 4063 4289 4441 6550 1493
Size: 1656 565 349 226 152 2109 1697
Fragments arranged by size:
2109 1697 1656 565 349 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5308 160
Size: 872 4276 1606
Fragments arranged by size:
4276 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
AvaI C'yCGr_G
Cuts at: 1870 1943 2614 4079 1870
Size: 73 671 1465 4545
Fragments arranged by size:
4545 1465 671 73
AvrII C'CTAG_G
Cuts at: 2719 2719
Size: 6754
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3486 3519 3486
Size: 33 6721
BamHI G'GATC_C
Cuts at: 4084 4451 4084
Size: 367 6387
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3055 3403 4075 5530 618
Size: 325 217 627 1268 348 672 1455 1842
Fragments arranged by size:
1842 1455 1268 672 627 348 325 217
BanII G_rGCy'C
Cuts at: 1391 2280 2889 3818 3966 4073 1391
Size: 889 609 929 148 107 4072
Fragments arranged by size:
4072 929 889 609 148 107
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2769 3032 3539 3873 928
Size: 971 870 263 507 334 3809
Fragments arranged by size:
3809 971 870 507 334 263
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4945 4979 1903
Size: 34 50 34 2924 34 3678
Fragments arranged by size:
3678 2924 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3506 4646 5856 3506
Size: 1140 1210 4404
Fragments arranged by size:
4404 1210 1140
BclI T'GATC_A
Cuts at: 1548 2097 2628 1548
Size: 549 531 5674
Fragments arranged by size:
5674 549 531
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5365 136
Size: 107 122 71 631 605 3693 1525
Fragments arranged by size:
3693 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2691 6749 2691
Size: 4058 2696
BlpI GC'TnA_GC
Cuts at: 4171 4171
Size: 6754
Bme1580I G_kGCm'C
Cuts at: 1790 2280 3408 4815 5971 1790
Size: 490 1128 1407 1156 2573
Fragments arranged by size:
2573 1407 1156 1128 490
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6754
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4485 5443 442
Size: 416 53 83 399 24 3068 958 1753
Fragments arranged by size:
3068 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6754
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3279 5414 1239
Size: 2040 2135 2579
Fragments arranged by size:
2579 2135 2040
Bpu10I CC'TnA_GC
Cuts at: 2768 2768
Size: 6754
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4879 5744 6042 4879
Size: 865 298 5591
Fragments arranged by size:
5591 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5417 882
Size: 617 3918 2219
Fragments arranged by size:
3918 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2466 3424 4545 493
Size: 711 1262 958 1121 2702
Fragments arranged by size:
2702 1262 1121 958 711
BsaBI GATnn'nnATC
Cuts at: 2060 2320 6748 2060
Size: 260 4428 2066
Fragments arranged by size:
4428 2066 260
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4561 4943 5578 275
Size: 53 83 186 936 3028 382 635 1451
Fragments arranged by size:
3028 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5187 5585 5859 6006 6417 5187
Size: 398 274 147 411 5524
Fragments arranged by size:
5524 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2964 2994 3747 3777 1178
Size: 30 165 30 1561 30 753 30 4155
Fragments arranged by size:
4155 1561 753 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2178 4008 1186
Size: 992 1830 3932
Fragments arranged by size:
3932 1830 992
BseYI C'CCAG_C
Cuts at: 5957 6453 5957
Size: 496 6258
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6181
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3622 4204 4965 5993 1540
Size: 275 186 1621 582 761 1028 2301
Fragments arranged by size:
2301 1621 1028 761 582 275 186
BsiHKAI G_wGCw'C
Cuts at: 2170 2733 4044 4815 4900 5971 2170
Size: 563 1311 771 85 1071 2953
Fragments arranged by size:
2953 1311 1071 771 563 85
BsmI GAATG_Cn'
Cuts at: 3350 4265 4358 3350
Size: 915 93 5746
Fragments arranged by size:
5746 915 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2170 2280 2733 2889 3408 3818 3966
Size: 399 380 110 453 156 519 410 148
Cuts at: 3966 4044 4073 4815 4900 5971 1391
Size: 78 29 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 519 453 410 399 380
156 148 110 85 78 29
BspEI T'CCGG_A
Cuts at: 6417 6417
Size: 6754
BspHI T'CATG_A
Cuts at: 3285 4643 6373 3285
Size: 1358 1730 3666
Fragments arranged by size:
3666 1730 1358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4109 844
Size: 3265 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4641 1394
Size: 429 2818 3507
Fragments arranged by size:
3507 2818 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3312 3660 5249 5423 65
Size: 1183 2064 348 1589 174 1396
Fragments arranged by size:
2064 1589 1396 1183 348 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5398 5585 1700
Size: 110 3588 187 2869
Fragments arranged by size:
3588 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2947 2961 3359 3721 96
Size: 2851 14 398 362 3129
Fragments arranged by size:
3129 2851 398 362 14
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6754
BssSI C'ACGA_G
Cuts at: 4814 5826 4814
Size: 1012 5742
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6754
BstBI TT'CG_AA
Cuts at: 5558 5558
Size: 6754
BstEII G'GTnAC_C
Cuts at: 1184 2801 1184
Size: 1617 5137
BstYI r'GATC_y
Cuts at: 1972 2691 3075 3216 4084 4451 4836 4853 6305
Size: 719 384 141 868 367 385 17 1452
Cuts at: 6305 6391 6403 6749 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 868 719 385 384 367 346
141 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2547 3051 513
Size: 552 1482 504 4216
Fragments arranged by size:
4216 1482 552 504
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6015
Fragments arranged by size:
6015 683 56
BtsI GCAGTG_nn'
Cuts at: 4270 5064 5084 4270
Size: 794 20 5940
Fragments arranged by size:
5940 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6754
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6719
DraI TTT'AAA
Cuts at: 4058 4058
Size: 6754
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2405 2771 5761 809
Size: 507 1089 366 2990 1802
Fragments arranged by size:
2990 1802 1089 507 366
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2904 4201 5090
Size: 54 1425 50 275 18 1074 1297 889
Cuts at: 5090 6719 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1297 1074 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4201 1537
Size: 275 2389 4090
Fragments arranged by size:
4090 2389 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2130 2672 4684 1353
Size: 777 542 2012 3423
Fragments arranged by size:
3423 2012 777 542
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3249 5341 5715 5861 387
Size: 1064 138 1660 2092 374 146 1280
Fragments arranged by size:
2092 1660 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2642 2681 2719 3264 3279 3539 3629
Size: 393 1010 39 38 545 15 260 90
Cuts at: 3629 4817 5414 6201 6469 1239
Size: 1188 597 787 268 1524
Fragments arranged by size:
1524 1188 1010 787 597 545 393 268
260 90 39 38 15
EcoO109I rG'GnC_Cy
Cuts at: 2277 3523 2277
Size: 1246 5508
EcoRI G'AATT_C
Cuts at: 4063 4441 4063
Size: 378 6376
EcoRV GAT'ATC
Cuts at: 2542 2542
Size: 6754
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3127 3139 3223 3835 3127
Size: 12 84 612 6046
Fragments arranged by size:
6046 612 84 12
FspI TGC'GCA
Cuts at: 5260 5260
Size: 6754
HaeII r_GCGC'y
Cuts at: 1678 5901 1678
Size: 4223 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2067 2099 2681 2713 2812
Size: 32 135 32 495 32 582 32 99
Cuts at: 2812 2844 3371 3376 3403 3408 5395 5427 5469
Size: 32 527 5 27 5 1987 32 42
Cuts at: 5469 5501 1373
Size: 32 2626
Fragments arranged by size:
2626 1987 582 527 495 135 99 42
32 32 32 32 32 32 32 27
5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2530 3701 4098 4344 669
Size: 988 281 592 1171 397 246 3079
Fragments arranged by size:
3079 1171 988 592 397 281 246
HindIII A'AGCT_T
Cuts at: 748 3327 3800 748
Size: 2579 473 3702
Fragments arranged by size:
3702 2579 473
HpaI GTT'AAC
Cuts at: 4344 4344
Size: 6754
KpnI G_GTAC'C
Cuts at: 4079 4079
Size: 6754
MfeI C'AATT_G
Cuts at: 4353 4353
Size: 6754
MluI A'CGCG_T
Cuts at: 6630 6630
Size: 6754
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2066 2962 5868 6052 1305
Size: 761 896 2906 184 2007
Fragments arranged by size:
2906 2007 896 761 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2906 10
Size: 54 1425 343 1074 3858
Fragments arranged by size:
3858 1425 1074 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2636 2880 4712 5071 5230 518
Size: 648 1470 244 1832 359 159 2042
Fragments arranged by size:
2042 1832 1470 648 359 244 159
MspA1I CmG'CkG
Cuts at: 1606 2967 3053 4170 4847 5995 6240 1606
Size: 1361 86 1117 677 1148 245 2120
Fragments arranged by size:
2120 1361 1148 1117 677 245 86
NcoI C'CATG_G
Cuts at: 513 1065 2547 513
Size: 552 1482 4720
Fragments arranged by size:
4720 1482 552
NdeI CA'TA_TG
Cuts at: 387 2931 387
Size: 2544 4210
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6754
NotI GC'GGCC_GC
Cuts at: 4201 4201
Size: 6754
NspI r_CATG'y
Cuts at: 1132 1229 2879 4112 5657 1132
Size: 97 1650 1233 1545 2229
Fragments arranged by size:
2229 1650 1545 1233 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6754
PciI A'CATG_T
Cuts at: 5653 5653
Size: 6754
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6754
PfoI T'CCnGG_A
Cuts at: 3219 4047 3219
Size: 828 5926
PmeI GTTT'AAAC
Cuts at: 4058 4058
Size: 6754
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3747 3779 4823 4855 6336 6368 3747
Size: 32 1044 32 1481 32 4133
Fragments arranged by size:
4133 1481 1044 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3523 3523
Size: 6754
PshAI GACnn'nnGTC
Cuts at: 1613 3582 1613
Size: 1969 4785
PsiI TTA'TAA
Cuts at: 2385 4324 2385
Size: 1939 4815
PspOMI G'GGCC_C
Cuts at: 2276 2276
Size: 6754
PstI C_TGCA'G
Cuts at: 830 4106 830
Size: 3276 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6754
PvuII CAG'CTG
Cuts at: 2967 2967
Size: 6754
SacII CC_GC'GG
Cuts at: 3054 3054
Size: 6754
SalI G'TCGA_C
Cuts at: 1936 4096 1936
Size: 2160 4594
SbfI CC_TGCA'GG
Cuts at: 4106 4106
Size: 6754
ScaI AGT'ACT
Cuts at: 5002 6714 5002
Size: 1712 5042
SfcI C'TryA_G
Cuts at: 826 1045 2311 4102 5237 5918 6109 826
Size: 219 1266 1791 1135 681 191 1471
Fragments arranged by size:
1791 1471 1266 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6754
SmaI CCC'GGG
Cuts at: 1872 4081 1872
Size: 2209 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4858 5759 6021 820
Size: 197 926 2915 901 262 1553
Fragments arranged by size:
2915 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4545 493
Size: 4052 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6754
SphI G_CATG'C
Cuts at: 1132 2879 4112 1132
Size: 1747 1233 3774
Fragments arranged by size:
3774 1747 1233
SspI AAT'ATT
Cuts at: 5 52 2836 4678 5
Size: 47 2784 1842 2081
Fragments arranged by size:
2784 2081 1842 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6754
StyI C'CwwG_G
Cuts at: 513 1065 2499 2547 2719 2992 3649 4193 513
Size: 552 1434 48 172 273 657 544 3074
Fragments arranged by size:
3074 1434 657 552 544 273 172 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4781 4798 4951 5136 5581 325
Size: 1255 3201 17 153 185 445 1498
Fragments arranged by size:
3201 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2947 2961 3359
Size: 274 80 33 51 1443 970 14 398
Cuts at: 3359 3721 3778 5000 6712 96
Size: 362 57 1222 1712 138
Fragments arranged by size:
1712 1443 1222 970 398 362 274 138
80 57 51 33 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2247 2279 2785 2817 3031 3063 3303 3335 3877
Size: 32 506 32 214 32 240 32 542
Cuts at: 3877 3909 6714 6746 2247
Size: 32 2805 32 2255
Fragments arranged by size:
2805 2255 542 506 240 214 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6754
XbaI T'CTAG_A
Cuts at: 4090 4090
Size: 6754
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6754
XmaI C'CCGG_G
Cuts at: 1870 4079 1870
Size: 2209 4545
XmnI GAAnn'nnTTC
Cuts at: 2606 3119 3635 4883 2606
Size: 513 516 1248 4477
Fragments arranged by size:
4477 1248 516 513
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4562 276
Size: 53 83 186 936 3028 2468
Fragments arranged by size:
3028 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BbeI BbvCI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI