Restriction map for
pFN21AE4557
(Circular) (Six-base) MAPSORT of: pFN21AE4557.seq Check: 3960 from: 1 to: 6742
pFN21AE4557 6742 bp
With 182 enzymes: SgfI *
March 28, 2012 14:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4552 278
Size: 53 83 186 936 3016 2468
Fragments arranged by size:
3016 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2648 4085 1937
Size: 711 1437 4594
Fragments arranged by size:
4594 1437 711
Acc65I G'GTAC_C
Cuts at: 2693 4063 2693
Size: 1370 5372
AclI AA'CG_TT
Cuts at: 1563 4869 5242 1563
Size: 3306 373 3063
Fragments arranged by size:
3306 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2201 3552 3787 4003 4805 6189 6457 1632
Size: 569 1351 235 216 802 1384 268 1917
Fragments arranged by size:
1917 1384 1351 802 569 268 235 216
AflII C'TTAA_G
Cuts at: 820 1017 3238 820
Size: 197 2221 4324
Fragments arranged by size:
4324 2221 197
AflIII A'CryG_T
Cuts at: 1203 5641 6618 1203
Size: 4438 977 1327
Fragments arranged by size:
4438 1327 977
AgeI A'CCGG_T
Cuts at: 5573 5573
Size: 6742
AhdI GACnn_n'nnGTC
Cuts at: 5471 5471
Size: 6742
AleI CACnn'nnGTG
Cuts at: 1166 3833 1166
Size: 2667 4075
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6742
AlwNI CAG_nnn'CTG
Cuts at: 3405 3437 3556 4485 6057 3405
Size: 32 119 929 1572 4090
Fragments arranged by size:
4090 1572 929 119 32
ApaLI G'TGCA_C
Cuts at: 4799 5955 4799
Size: 1156 5586
ApoI r'AATT_y
Cuts at: 1493 2140 2526 3378 4051 4277 4429 6538 1493
Size: 647 386 852 673 226 152 2109 1697
Fragments arranged by size:
2109 1697 852 673 647 386 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5296 160
Size: 872 4264 1606
Fragments arranged by size:
4264 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6742
AvaI C'yCGr_G
Cuts at: 1870 1943 4067 1870
Size: 73 2124 4545
Fragments arranged by size:
4545 2124 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2142 2175 2142
Size: 33 6709
BamHI G'GATC_C
Cuts at: 4072 4439 4072
Size: 367 6375
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2693 4063 5518 618
Size: 325 217 627 906 1370 1455 1842
Fragments arranged by size:
1842 1455 1370 906 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2165 2225 3890 4061 1391
Size: 774 60 1665 171 4072
Fragments arranged by size:
4072 1665 774 171 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2850 928
Size: 971 951 4820
Fragments arranged by size:
4820 971 951
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4933 4967 1903
Size: 34 2996 34 3678
Fragments arranged by size:
3678 2996 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4634 5844 4634
Size: 1210 5532
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6742
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4010 5353 136
Size: 107 122 71 631 605 2338 1343 1525
Fragments arranged by size:
2338 1525 1343 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6737 6737
Size: 6742
BlpI GC'TnA_GC
Cuts at: 4159 4159
Size: 6742
Bme1580I G_kGCm'C
Cuts at: 1790 2734 2925 4803 5959 1790
Size: 944 191 1878 1156 2573
Fragments arranged by size:
2573 1878 1156 944 191
BmgBI CAC'GTC
Cuts at: 1318 2278 1318
Size: 960 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2243 4473 5431
Size: 416 53 83 399 24 826 2230 958
Cuts at: 5431 442
Size: 1753
Fragments arranged by size:
2230 1753 958 826 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2205 1056
Size: 1149 5593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2673 2912 2970 3103 4008 5402 1239
Size: 1434 239 58 133 905 1394 2579
Fragments arranged by size:
2579 1434 1394 905 239 133 58
Bpu10I CC'TnA_GC
Cuts at: 2164 2164
Size: 6742
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2283 4867 5732 6030 2283
Size: 2584 865 298 2995
Fragments arranged by size:
2995 2584 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5405 882
Size: 617 3906 2219
Fragments arranged by size:
3906 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4533 493
Size: 711 3329 2702
Fragments arranged by size:
3329 2702 711
BsaBI GATnn'nnATC
Cuts at: 2078 6736 2078
Size: 4658 2084
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3937 4549 4931 5566
Size: 53 83 186 936 2404 612 382 635
Cuts at: 5566 275
Size: 1451
Fragments arranged by size:
2404 1451 936 635 612 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2259 3224 3912 5175 5573 5847 5994 6405 2259
Size: 965 688 1263 398 274 147 411 2596
Fragments arranged by size:
2596 1263 965 688 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6517
Fragments arranged by size:
6517 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2957 3533 1186
Size: 1771 576 4395
Fragments arranged by size:
4395 1771 576
BseYI C'CCAG_C
Cuts at: 2665 3005 5945 6441 2665
Size: 340 2940 496 2966
Fragments arranged by size:
2966 2940 496 340
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2599 2739 3075 3080 1163
Size: 573 863 140 336 5 4825
Fragments arranged by size:
4825 863 573 336 140 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4192 4953 5981 1540
Size: 275 186 2191 761 1028 2301
Fragments arranged by size:
2301 2191 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2659 4803 4888 5959 2659
Size: 2144 85 1071 3442
Fragments arranged by size:
3442 2144 1071 85
BsmI GAATG_Cn'
Cuts at: 2111 2549 4253 4346 2111
Size: 438 1704 93 4507
Fragments arranged by size:
4507 1704 438 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3944 3944
Size: 6742
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2165 2225 2659 2734 2925 3890 4061
Size: 399 375 60 434 75 191 965 171
Cuts at: 4061 4803 4888 5959 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 965 742 434 399 375 191
171 85 75 60
BspEI T'CCGG_A
Cuts at: 2259 6405 2259
Size: 4146 2596
BspHI T'CATG_A
Cuts at: 4631 6361 4631
Size: 1730 5012
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4097 844
Size: 3253 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4629 1394
Size: 429 2806 3507
Fragments arranged by size:
3507 2806 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3415 3607 3619 5237 5411 65
Size: 1183 2167 192 12 1618 174 1396
Fragments arranged by size:
2167 1618 1396 1183 192 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 2315 5386 5573 1700
Size: 110 505 3071 187 2869
Fragments arranged by size:
3071 2869 505 187 110
BsrGI T'GTAC_A
Cuts at: 96 2286 3165 96
Size: 2190 879 3673
Fragments arranged by size:
3673 2190 879
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6742
BssSI C'ACGA_G
Cuts at: 2357 2429 4802 5814 2357
Size: 72 2373 1012 3285
Fragments arranged by size:
3285 2373 1012 72
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6742
BstBI TT'CG_AA
Cuts at: 3382 5546 3382
Size: 2164 4578
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6742
BstYI r'GATC_y
Cuts at: 1972 2569 3561 3772 4072 4439 4824 4841 6293
Size: 597 992 211 300 367 385 17 1452
Cuts at: 6293 6379 6391 6737 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 992 597 385 367 346 300
211 86 17 12
BstZ17I GTA'TAC
Cuts at: 2649 2649
Size: 6742
BtgI C'CryG_G
Cuts at: 513 1065 2004 2118 3270 3592 3762 513
Size: 552 939 114 1152 322 170 3493
Fragments arranged by size:
3493 1152 939 552 322 170 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6003
Fragments arranged by size:
6003 683 56
BtsI GCAGTG_nn'
Cuts at: 2501 3063 4258 5052 5072 2501
Size: 562 1195 794 20 4171
Fragments arranged by size:
4171 1195 794 562 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6742
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6707
DraI TTT'AAA
Cuts at: 4046 4046
Size: 6742
DraIII CAC_nnn'GTG
Cuts at: 3145 3145
Size: 6742
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3452 5749 809
Size: 507 2136 2297 1802
Fragments arranged by size:
2297 2136 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2313 3124 4189
Size: 54 1425 50 275 18 483 811 1065
Cuts at: 4189 5078 6707 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1065 889 811 483 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4189 1537
Size: 275 2377 4090
Fragments arranged by size:
4090 2377 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2762 3551 3742 4672 1353
Size: 1409 789 191 930 3423
Fragments arranged by size:
3423 1409 930 789 191
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5329 5703 5849 387
Size: 1064 138 3740 374 146 1280
Fragments arranged by size:
3740 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2673 2912 2970 3103 3552 3787
Size: 393 569 472 239 58 133 449 235
Cuts at: 3787 4003 4008 4805 5402 6189 6457 1239
Size: 216 5 797 597 787 268 1524
Fragments arranged by size:
1524 797 787 597 569 472 449 393
268 239 235 216 133 58 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2561 2561
Size: 6742
EcoO109I rG'GnC_Cy
Cuts at: 2909 2909
Size: 6742
EcoRI G'AATT_C
Cuts at: 3378 4051 4429 3378
Size: 673 378 5691
Fragments arranged by size:
5691 673 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2851 2851
Size: 6742
FspI TGC'GCA
Cuts at: 5248 5248
Size: 6742
HaeII r_GCGC'y
Cuts at: 1678 5889 1678
Size: 4211 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3413 3445 4014 4046 5383
Size: 32 135 32 1841 32 569 32 1337
Cuts at: 5383 5415 5457 5489 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1841 1337 569 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3931 4086 4332 669
Size: 988 281 1993 155 246 3079
Fragments arranged by size:
3079 1993 988 281 246 155
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6742
HpaI GTT'AAC
Cuts at: 4332 4332
Size: 6742
KpnI G_GTAC'C
Cuts at: 2697 4067 2697
Size: 1370 5372
MfeI C'AATT_G
Cuts at: 4341 4341
Size: 6742
MluI A'CGCG_T
Cuts at: 6618 6618
Size: 6742
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2602 5856 6040 1305
Size: 1297 3254 184 2007
Fragments arranged by size:
3254 2007 1297 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3126 10
Size: 54 1425 343 1294 3626
Fragments arranged by size:
3626 1425 1294 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2708 3688 3833 3956 4700 5059 5218
Size: 648 1542 980 145 123 744 359 159
Cuts at: 5218 518
Size: 2042
Fragments arranged by size:
2042 1542 980 744 648 359 159 145
123
MspA1I CmG'CkG
Cuts at: 1606 2033 2156 4158 4835 5983 6228 1606
Size: 427 123 2002 677 1148 245 2120
Fragments arranged by size:
2120 2002 1148 677 427 245 123
NaeI GCC'GGC
Cuts at: 2317 2317
Size: 6742
NcoI C'CATG_G
Cuts at: 513 1065 2004 3270 3592 3762 513
Size: 552 939 1266 322 170 3493
Fragments arranged by size:
3493 1266 939 552 322 170
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6742
NgoMIV G'CCGG_C
Cuts at: 2315 2315
Size: 6742
NheI G'CTAG_C
Cuts at: 1052 2201 1052
Size: 1149 5593
NotI GC'GGCC_GC
Cuts at: 4189 4189
Size: 6742
NspI r_CATG'y
Cuts at: 1132 1229 3289 4100 5645 1132
Size: 97 2060 811 1545 2229
Fragments arranged by size:
2229 2060 1545 811 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6742
PciI A'CATG_T
Cuts at: 5641 5641
Size: 6742
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2935 3763 1108
Size: 1827 828 4087
Fragments arranged by size:
4087 1827 828
PmeI GTTT'AAAC
Cuts at: 4046 4046
Size: 6742
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4811 4843 6324 6356 4811
Size: 32 1481 32 5197
Fragments arranged by size:
5197 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3936 1613
Size: 2323 4419
PsiI TTA'TAA
Cuts at: 4312 4312
Size: 6742
PstI C_TGCA'G
Cuts at: 830 3561 4094 830
Size: 2731 533 3478
Fragments arranged by size:
3478 2731 533
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6742
PvuII CAG'CTG
Cuts at: 2033 2156 2033
Size: 123 6619
SalI G'TCGA_C
Cuts at: 1936 4084 1936
Size: 2148 4594
SbfI CC_TGCA'GG
Cuts at: 4094 4094
Size: 6742
ScaI AGT'ACT
Cuts at: 2026 4990 6702 2026
Size: 2964 1712 2066
Fragments arranged by size:
2964 2066 1712
SfcI C'TryA_G
Cuts at: 826 1045 2028 3557 4090 5225 5906 6097 826
Size: 219 983 1529 533 1135 681 191 1471
Fragments arranged by size:
1529 1471 1135 983 681 533 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6742
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6742
SmaI CCC'GGG
Cuts at: 1872 4069 1872
Size: 2197 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2298 3238 4846 5747 6009 820
Size: 197 926 355 940 1608 901 262 1553
Fragments arranged by size:
1608 1553 940 926 901 355 262 197
SnaBI TAC'GTA
Cuts at: 493 4533 493
Size: 4040 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6742
SphI G_CATG'C
Cuts at: 1132 3289 4100 1132
Size: 2157 811 3774
Fragments arranged by size:
3774 2157 811
SspI AAT'ATT
Cuts at: 5 52 3850 4666 5
Size: 47 3798 816 2081
Fragments arranged by size:
3798 2081 816 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6742
StyI C'CwwG_G
Cuts at: 513 1065 2004 3198 3270 3592 3762 4181 513
Size: 552 939 1194 72 322 170 419 3074
Fragments arranged by size:
3074 1194 939 552 419 322 170 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4769 4786 4939 5124 5569 325
Size: 1255 3189 17 153 185 445 1498
Fragments arranged by size:
3189 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2024 2286 3165
Size: 274 80 33 51 1443 47 262 879
Cuts at: 3165 3866 4988 6700 96
Size: 701 1122 1712 138
Fragments arranged by size:
1712 1443 1122 879 701 274 262 138
80 51 47 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6702 6734 6702
Size: 32 6710
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6742
XbaI T'CTAG_A
Cuts at: 3502 4078 3502
Size: 576 6166
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2402 3625 2402
Size: 1223 5519
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6742
XmaI C'CCGG_G
Cuts at: 1870 4067 1870
Size: 2197 4545
XmnI GAAnn'nnTTC
Cuts at: 4871 4871
Size: 6742
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4550 276
Size: 53 83 186 936 3016 2468
Fragments arranged by size:
3016 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI BsiWI BstXI Bsu36I EcoICRI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI