Restriction map for
pFN21AE4555
(Circular) (Six-base) MAPSORT of: pFN21AE4555.seq Check: 7758 from: 1 to: 6730
pFN21AE4555 6730 bp
With 182 enzymes: SgfI *
March 28, 2012 14:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4540 278
Size: 53 83 186 936 3004 2468
Fragments arranged by size:
3004 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4073 1937
Size: 2136 4594
Acc65I G'GTAC_C
Cuts at: 2630 4051 2630
Size: 1421 5309
AclI AA'CG_TT
Cuts at: 1563 4857 5230 1563
Size: 3294 373 3063
Fragments arranged by size:
3294 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4793 6177 6445 1632
Size: 3161 1384 268 1917
Fragments arranged by size:
3161 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6533
AflIII A'CryG_T
Cuts at: 1203 5629 6606 1203
Size: 4426 977 1327
Fragments arranged by size:
4426 1327 977
AgeI A'CCGG_T
Cuts at: 5561 5561
Size: 6730
AhdI GACnn_n'nnGTC
Cuts at: 5459 5459
Size: 6730
AleI CACnn'nnGTG
Cuts at: 1166 3038 1166
Size: 1872 4858
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6730
AlwNI CAG_nnn'CTG
Cuts at: 4473 6045 4473
Size: 1572 5158
ApaLI G'TGCA_C
Cuts at: 4787 5943 4787
Size: 1156 5574
ApoI r'AATT_y
Cuts at: 1493 2099 2234 2399 2453 2783 3257 3297 3962
Size: 606 135 165 54 330 474 40 665
Cuts at: 3962 4039 4265 4417 6526 1493
Size: 77 226 152 2109 1697
Fragments arranged by size:
2109 1697 665 606 474 330 226 165
152 135 77 54 40
AseI AT'TA_AT
Cuts at: 160 1032 2219 5284 160
Size: 872 1187 3065 1606
Fragments arranged by size:
3065 1606 1187 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6730
AvaI C'yCGr_G
Cuts at: 1870 1943 3402 3660 4055 1870
Size: 73 1459 258 395 4545
Fragments arranged by size:
4545 1459 395 258 73
BamHI G'GATC_C
Cuts at: 4060 4427 4060
Size: 367 6363
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2630 2729 3458 3584 4051
Size: 325 217 627 843 99 729 126 467
Cuts at: 4051 5506 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 843 729 627 467 325 217
126 99
BanII G_rGCy'C
Cuts at: 1391 4049 1391
Size: 2658 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5759
BbvCI CC'TCA_GC
Cuts at: 2922 2922
Size: 6730
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4921 4955 1903
Size: 34 2984 34 3678
Fragments arranged by size:
3678 2984 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3054 4622 5832 3054
Size: 1568 1210 3952
Fragments arranged by size:
3952 1568 1210
BclI T'GATC_A
Cuts at: 1548 3907 1548
Size: 2359 4371
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2021 5341
Size: 107 122 71 631 605 337 12 3320
Cuts at: 5341 136
Size: 1525
Fragments arranged by size:
3320 1525 631 605 337 122 107 71
12
BglII A'GATC_T
Cuts at: 2709 6725 2709
Size: 4016 2714
BlpI GC'TnA_GC
Cuts at: 2049 4147 2049
Size: 2098 4632
Bme1580I G_kGCm'C
Cuts at: 1790 2266 4791 5947 1790
Size: 476 2525 1156 2573
Fragments arranged by size:
2573 2525 1156 476
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6730
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2398 2538 3772
Size: 416 53 83 399 24 981 140 1234
Cuts at: 3772 4022 4461 5419 442
Size: 250 439 958 1753
Fragments arranged by size:
1753 1234 981 958 439 416 399 250
140 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2504 2646 3039 3327 3339 5390 1239
Size: 1265 142 393 288 12 2051 2579
Fragments arranged by size:
2579 2051 1265 393 288 142 12
Bpu10I CC'TnA_GC
Cuts at: 2863 2922 2863
Size: 59 6671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4855 5720 6018 4855
Size: 865 298 5567
Fragments arranged by size:
5567 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5393 882
Size: 617 3894 2219
Fragments arranged by size:
3894 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4521 493
Size: 711 3317 2702
Fragments arranged by size:
3317 2702 711
BsaBI GATnn'nnATC
Cuts at: 2221 6724 2221
Size: 4503 2227
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4537 4919 5554 275
Size: 53 83 186 936 3004 382 635 1451
Fragments arranged by size:
3004 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5163 5561 5835 5982 6393 5163
Size: 398 274 147 411 5500
Fragments arranged by size:
5500 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4014 4044 1178
Size: 30 165 30 2611 30 3864
Fragments arranged by size:
3864 2611 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2774 2877 3855 1186
Size: 1588 103 978 4061
Fragments arranged by size:
4061 1588 978 103
BseYI C'CCAG_C
Cuts at: 5933 6429 5933
Size: 496 6234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6157
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 4180 4941 5969 1540
Size: 275 186 12 2167 761 1028 2301
Fragments arranged by size:
2301 2167 1028 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 2490 3415 3757 4791 4876 5947 2490
Size: 925 342 1034 85 1071 3273
Fragments arranged by size:
3273 1071 1034 925 342 85
BsmI GAATG_Cn'
Cuts at: 3075 4241 4334 3075
Size: 1166 93 5471
Fragments arranged by size:
5471 1166 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2266 2490 3415 3757 4049 4791 4876
Size: 399 476 224 925 342 292 742 85
Cuts at: 4876 5947 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 925 742 476 399 342 292
224 85
BspEI T'CCGG_A
Cuts at: 6393 6393
Size: 6730
BspHI T'CATG_A
Cuts at: 4619 6349 4619
Size: 1730 5000
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3544 3897 4085 844
Size: 2700 353 188 3489
Fragments arranged by size:
3489 2700 353 188
BsrBI CCG'CTC
Cuts at: 1394 1823 4617 1394
Size: 429 2794 3507
Fragments arranged by size:
3507 2794 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5225 5399 65
Size: 1183 3977 174 1396
Fragments arranged by size:
3977 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5374 5561 1700
Size: 110 3564 187 2869
Fragments arranged by size:
3564 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6730
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6730
BssSI C'ACGA_G
Cuts at: 3756 4790 5802 3756
Size: 1034 1012 4684
Fragments arranged by size:
4684 1034 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6730
BstBI TT'CG_AA
Cuts at: 5534 5534
Size: 6730
BstEII G'GTnAC_C
Cuts at: 1184 2566 1184
Size: 1382 5348
BstXI CCAn_nnnn'nTGG
Cuts at: 2388 2388
Size: 6730
BstYI r'GATC_y
Cuts at: 1972 2041 2709 3363 3447 4060 4427 4812 4829
Size: 69 668 654 84 613 367 385 17
Cuts at: 4829 6281 6367 6379 6725 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 668 654 613 385 367 346
86 84 69 17 12
Bsu36I CC'TnA_GG
Cuts at: 2944 2944
Size: 6730
BtgI C'CryG_G
Cuts at: 513 1065 2004 2802 3852 513
Size: 552 939 798 1050 3391
Fragments arranged by size:
3391 1050 939 798 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3832 487
Size: 683 56 2606 3385
Fragments arranged by size:
3385 2606 683 56
BtsI GCAGTG_nn'
Cuts at: 3033 4246 5040 5060 3033
Size: 1213 794 20 4703
Fragments arranged by size:
4703 1213 794 20
ClaI AT'CG_AT
Cuts at: 1554 3051 1554
Size: 1497 5233
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2157 2192 3180 3215 529
Size: 35 1593 35 988 35 4044
Fragments arranged by size:
4044 1593 988 35 35 35
DraI TTT'AAA
Cuts at: 4034 4034
Size: 6730
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2564 5737 809
Size: 507 1248 3173 1802
Fragments arranged by size:
3173 1802 1248 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 3495 3667
Size: 54 1425 50 275 18 180 1485 172
Cuts at: 3667 4177 5066 6695 8
Size: 510 889 1629 43
Fragments arranged by size:
1629 1485 1425 889 510 275 180 172
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2010 4177 1537
Size: 275 198 2167 4090
Fragments arranged by size:
4090 2167 275 198
EarI CTCTTCn'nnn_
Cuts at: 1353 2889 3989 4660 1353
Size: 1536 1100 671 3423
Fragments arranged by size:
3423 1536 1100 671
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2564 5317 5691 5837 387
Size: 1064 138 975 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 975 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2504 2646 3039 3327 3339 4793 5390
Size: 393 872 142 393 288 12 1454 597
Cuts at: 5390 6177 6445 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1454 872 787 597 393 393 288
268 142 12
EcoO109I rG'GnC_Cy
Cuts at: 3430 3430
Size: 6730
EcoRI G'AATT_C
Cuts at: 4039 4417 4039
Size: 378 6352
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2518 3550 2518
Size: 1032 5698
FspI TGC'GCA
Cuts at: 5236 5236
Size: 6730
HaeII r_GCGC'y
Cuts at: 1678 5877 1678
Size: 4199 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2026 2058 2872 2904 5371
Size: 32 135 32 454 32 814 32 2467
Cuts at: 5371 5403 5445 5477 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2467 814 454 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2812 4074 4320 669
Size: 988 281 874 1262 246 3079
Fragments arranged by size:
3079 1262 988 874 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6730
HpaI GTT'AAC
Cuts at: 4320 4320
Size: 6730
KpnI G_GTAC'C
Cuts at: 2634 4055 2634
Size: 1421 5309
MfeI C'AATT_G
Cuts at: 4329 4329
Size: 6730
MluI A'CGCG_T
Cuts at: 6606 6606
Size: 6730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3007 5844 6028 1305
Size: 1702 2837 184 2007
Fragments arranged by size:
2837 2007 1702 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3669 10
Size: 54 1425 343 1837 3071
Fragments arranged by size:
3071 1837 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3038 3270 4688 5047 5206 518
Size: 648 1872 232 1418 359 159 2042
Fragments arranged by size:
2042 1872 1418 648 359 232 159
MspA1I CmG'CkG
Cuts at: 1606 2048 2296 3684 4146 4823 5971 6216 1606
Size: 442 248 1388 462 677 1148 245 2120
Fragments arranged by size:
2120 1388 1148 677 462 442 248 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2802 3852 513
Size: 552 939 798 1050 3391
Fragments arranged by size:
3391 1050 939 798 552
NdeI CA'TA_TG
Cuts at: 387 2446 387
Size: 2059 4671
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6730
NotI GC'GGCC_GC
Cuts at: 2010 4177 2010
Size: 2167 4563
NspI r_CATG'y
Cuts at: 1132 1229 3471 4088 5633 1132
Size: 97 2242 617 1545 2229
Fragments arranged by size:
2242 2229 1545 617 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6730
PciI A'CATG_T
Cuts at: 5629 5629
Size: 6730
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6730
PfoI T'CCnGG_A
Cuts at: 3878 3878
Size: 6730
PmeI GTTT'AAAC
Cuts at: 4034 4034
Size: 6730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4799 4831 6312 6344 4799
Size: 32 1481 32 5185
Fragments arranged by size:
5185 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3430 3430
Size: 6730
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6730
PsiI TTA'TAA
Cuts at: 4300 4300
Size: 6730
PspXI vC'TCGA_Gb
Cuts at: 3402 3402
Size: 6730
PstI C_TGCA'G
Cuts at: 830 3607 4082 830
Size: 2777 475 3478
Fragments arranged by size:
3478 2777 475
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6730
PvuII CAG'CTG
Cuts at: 2048 2296 2048
Size: 248 6482
SalI G'TCGA_C
Cuts at: 1936 4072 1936
Size: 2136 4594
SbfI CC_TGCA'GG
Cuts at: 4082 4082
Size: 6730
ScaI AGT'ACT
Cuts at: 4978 6690 4978
Size: 1712 5018
SfcI C'TryA_G
Cuts at: 826 1045 2713 2992 3603 3812 4078 5213 5894
Size: 219 1668 279 611 209 266 1135 681
Cuts at: 5894 6085 826
Size: 191 1471
Fragments arranged by size:
1668 1471 1135 681 611 279 266 219
209 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6730
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6730
SmaI CCC'GGG
Cuts at: 1872 3662 4057 1872
Size: 1790 395 4545
Fragments arranged by size:
4545 1790 395
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3402 4834 5735 5997 820
Size: 197 926 1459 1432 901 262 1553
Fragments arranged by size:
1553 1459 1432 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4521 493
Size: 4028 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6730
SphI G_CATG'C
Cuts at: 1132 3471 4088 1132
Size: 2339 617 3774
Fragments arranged by size:
3774 2339 617
SrfI GCCC'GGGC
Cuts at: 3662 3662
Size: 6730
SspI AAT'ATT
Cuts at: 5 52 2064 4654 5
Size: 47 2012 2590 2081
Fragments arranged by size:
2590 2081 2012 47
StuI AGG'CCT
Cuts at: 1517 2942 1517
Size: 1425 5305
StyI C'CwwG_G
Cuts at: 513 1065 2004 2016 2802 2846 3852 4169 513
Size: 552 939 12 786 44 1006 317 3074
Fragments arranged by size:
3074 1006 939 786 552 317 44 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2815 3268 4757 4774 4927 5112 5557
Size: 1255 1235 453 1489 17 153 185 445
Cuts at: 5557 325
Size: 1498
Fragments arranged by size:
1498 1489 1255 1235 453 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4976 6688 96
Size: 274 80 33 51 1443 2999 1712 138
Fragments arranged by size:
2999 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3382 3414 6690 6722 3382
Size: 32 3276 32 3390
Fragments arranged by size:
3390 3276 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2123 1316
Size: 807 5923
XbaI T'CTAG_A
Cuts at: 4066 4066
Size: 6730
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3086 3735 3086
Size: 649 6081
XhoI C'TCGA_G
Cuts at: 1943 3402 1943
Size: 1459 5271
XmaI C'CCGG_G
Cuts at: 1870 3660 4055 1870
Size: 1790 395 4545
Fragments arranged by size:
4545 1790 395
XmnI GAAnn'nnTTC
Cuts at: 4859 4859
Size: 6730
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4538 276
Size: 53 83 186 936 3004 2468
Fragments arranged by size:
3004 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SwaI