(Circular) (Six-base) MAPSORT of: pFN21AE4553.seq Check: 1577 from: 1 to: 6700 pFN21AE4553 6700 bp With 182 enzymes: SgfI * March 28, 2012 14:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4510 278 Size: 53 83 186 936 2974 2468 Fragments arranged by size: 2974 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4043 1937 Size: 2106 4594 Acc65I G'GTAC_C Cuts at: 4021 4021 Size: 6700 AclI AA'CG_TT Cuts at: 1563 2779 4827 5200 1563 Size: 1216 2048 373 3063 Fragments arranged by size: 3063 2048 1216 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2040 3677 3809 3871 3971 4763 6147 6415 Size: 408 1637 132 62 100 792 1384 268 Cuts at: 6415 1632 Size: 1917 Fragments arranged by size: 1917 1637 1384 792 408 268 132 100 62 AfeI AGC'GCT Cuts at: 2729 3986 2729 Size: 1257 5443 AflII C'TTAA_G Cuts at: 820 1017 3458 820 Size: 197 2441 4062 Fragments arranged by size: 4062 2441 197 AflIII A'CryG_T Cuts at: 1203 3910 5599 6576 1203 Size: 2707 1689 977 1327 Fragments arranged by size: 2707 1689 1327 977 AgeI A'CCGG_T Cuts at: 5531 5531 Size: 6700 AhdI GACnn_n'nnGTC Cuts at: 5429 5429 Size: 6700 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6700 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3162 1184 Size: 1978 4722 AlwNI CAG_nnn'CTG Cuts at: 2078 2396 3266 4443 6015 2078 Size: 318 870 1177 1572 2763 Fragments arranged by size: 2763 1572 1177 870 318 ApaI G_GGCC'C Cuts at: 2512 3379 2512 Size: 867 5833 ApaLI G'TGCA_C Cuts at: 4757 5913 4757 Size: 1156 5544 ApoI r'AATT_y Cuts at: 1493 4009 4235 4387 6496 1493 Size: 2516 226 152 2109 1697 Fragments arranged by size: 2516 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5254 160 Size: 872 4222 1606 Fragments arranged by size: 4222 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6700 AvaI C'yCGr_G Cuts at: 1870 1943 3210 3807 4025 1870 Size: 73 1267 597 218 4545 Fragments arranged by size: 4545 1267 597 218 73 BamHI G'GATC_C Cuts at: 4030 4397 4030 Size: 367 6333 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2489 3768 3992 4021 5476 Size: 325 217 627 702 1279 224 29 1455 Cuts at: 5476 618 Size: 1842 Fragments arranged by size: 1842 1455 1279 702 627 325 224 217 29 BanII G_rGCy'C Cuts at: 1391 2455 2512 3211 3242 3249 3379 3876 4019 Size: 1064 57 699 31 7 130 497 143 Cuts at: 4019 1391 Size: 4072 Fragments arranged by size: 4072 1064 699 497 143 130 57 31 7 BbeI G_GCGC'C Cuts at: 3772 3996 3772 Size: 224 6476 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2408 2607 3428 3815 928 Size: 971 509 199 821 387 3813 Fragments arranged by size: 3813 971 821 509 387 199 BbvCI CC'TCA_GC Cuts at: 3335 3507 3720 3335 Size: 172 213 6315 Fragments arranged by size: 6315 213 172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2586 2620 4891 4925 1903 Size: 34 649 34 2271 34 3678 Fragments arranged by size: 3678 2271 649 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3032 4592 5802 3032 Size: 1560 1210 3930 Fragments arranged by size: 3930 1560 1210 BclI T'GATC_A Cuts at: 1548 3108 1548 Size: 1560 5140 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3503 5311 136 Size: 107 122 71 631 605 1831 1808 1525 Fragments arranged by size: 1831 1808 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2538 3811 6695 2538 Size: 1273 2884 2543 Fragments arranged by size: 2884 2543 1273 BlpI GC'TnA_GC Cuts at: 2093 3010 3299 3802 4117 2093 Size: 917 289 503 315 4676 Fragments arranged by size: 4676 917 503 315 289 Bme1580I G_kGCm'C Cuts at: 1790 2512 3379 4761 5917 1790 Size: 722 867 1382 1156 2573 Fragments arranged by size: 2573 1382 1156 867 722 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6700 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4431 5389 442 Size: 416 53 83 399 24 3014 958 1753 Fragments arranged by size: 3014 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6700 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2316 2316 Size: 6700 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2109 2260 3243 3344 3500 3704 5360 1239 Size: 870 151 983 101 156 204 1656 2579 Fragments arranged by size: 2579 1656 983 870 204 156 151 101 Bpu10I CC'TnA_GC Cuts at: 3243 3335 3507 3720 3243 Size: 92 172 213 6223 Fragments arranged by size: 6223 213 172 92 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4825 5690 5988 4825 Size: 865 298 5537 Fragments arranged by size: 5537 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2577 2636 3974 5363 882 Size: 617 1078 59 1338 1389 2219 Fragments arranged by size: 2219 1389 1338 1078 617 59 BsaAI yAC'GTr Cuts at: 493 1204 4491 493 Size: 711 3287 2702 Fragments arranged by size: 3287 2702 711 BsaBI GATnn'nnATC Cuts at: 3650 6694 3650 Size: 3044 3656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3769 3993 4507 4889 Size: 53 83 186 936 2236 224 514 382 Cuts at: 4889 5524 275 Size: 635 1451 Fragments arranged by size: 2236 1451 936 635 514 382 224 186 83 53 BsaWI w'CCGG_w Cuts at: 5133 5531 5805 5952 6363 5133 Size: 398 274 147 411 5470 Fragments arranged by size: 5470 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2574 2604 2714 2744 3056 Size: 30 165 30 1171 30 110 30 312 Cuts at: 3056 3086 1178 Size: 30 4792 Fragments arranged by size: 4792 1171 312 165 110 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2308 2498 2722 3242 3323 3725 1186 Size: 1122 190 224 520 81 402 4161 Fragments arranged by size: 4161 1122 520 402 224 190 81 BseYI C'CCAG_C Cuts at: 2396 2416 2831 3729 3849 5903 6399 2396 Size: 20 415 898 120 2054 496 2697 Fragments arranged by size: 2697 2054 898 496 415 120 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2348 1163 Size: 573 612 5515 Fragments arranged by size: 5515 612 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4150 4911 5939 1540 Size: 275 186 2149 761 1028 2301 Fragments arranged by size: 2301 2149 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2754 3876 4761 4846 5917 2754 Size: 1122 885 85 1071 3537 Fragments arranged by size: 3537 1122 1071 885 85 BsmI GAATG_Cn' Cuts at: 2368 4211 4304 2368 Size: 1843 93 4764 Fragments arranged by size: 4764 1843 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3043 3043 Size: 6700 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2455 2512 2754 3211 3242 3249 3379 Size: 399 665 57 242 457 31 7 130 Cuts at: 3379 3876 4019 4761 4846 5917 1391 Size: 497 143 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 665 497 457 399 242 143 130 85 57 31 7 BspEI T'CCGG_A Cuts at: 6363 6363 Size: 6700 BspHI T'CATG_A Cuts at: 4589 6319 4589 Size: 1730 4970 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2309 4055 844 Size: 1465 1746 3489 Fragments arranged by size: 3489 1746 1465 BsrBI CCG'CTC Cuts at: 1394 1823 2671 3406 4587 1394 Size: 429 848 735 1181 3507 Fragments arranged by size: 3507 1181 848 735 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3036 5195 5369 65 Size: 1183 1788 2159 174 1396 Fragments arranged by size: 2159 1788 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2499 2847 3977 5344 5531 1700 Size: 110 689 348 1130 1367 187 2869 Fragments arranged by size: 2869 1367 1130 689 348 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6700 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6700 BssSI C'ACGA_G Cuts at: 2952 4760 5772 2952 Size: 1808 1012 3880 Fragments arranged by size: 3880 1808 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2413 2680 794 Size: 1619 267 4814 Fragments arranged by size: 4814 1619 267 BstBI TT'CG_AA Cuts at: 5504 5504 Size: 6700 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6700 BstXI CCAn_nnnn'nTGG Cuts at: 3493 3989 3493 Size: 496 6204 BstYI r'GATC_y Cuts at: 1972 2538 3645 3784 3811 4030 4397 4782 4799 Size: 566 1107 139 27 219 367 385 17 Cuts at: 4799 6251 6337 6349 6695 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1107 566 385 367 346 219 139 86 27 17 12 Bsu36I CC'TnA_GG Cuts at: 2339 3219 3708 2339 Size: 880 489 5331 Fragments arranged by size: 5331 880 489 BtgI C'CryG_G Cuts at: 513 1065 2004 2010 2447 2504 3249 3877 513 Size: 552 939 6 437 57 745 628 3336 Fragments arranged by size: 3336 939 745 628 552 437 57 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3539 487 Size: 683 56 2313 3648 Fragments arranged by size: 3648 2313 683 56 BtsI GCAGTG_nn' Cuts at: 4216 5010 5030 4216 Size: 794 20 5886 Fragments arranged by size: 5886 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6700 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6665 DraI TTT'AAA Cuts at: 4004 4004 Size: 6700 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5707 809 Size: 507 4391 1802 Fragments arranged by size: 4391 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2156 2501 Size: 54 1425 50 275 18 177 149 345 Cuts at: 2501 3315 3979 4147 5036 6665 8 Size: 814 664 168 889 1629 43 Fragments arranged by size: 1629 1425 889 814 664 345 275 177 168 149 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4147 1537 Size: 275 2335 4090 Fragments arranged by size: 4090 2335 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3219 3426 3668 4630 1353 Size: 1866 207 242 962 3423 Fragments arranged by size: 3423 1866 962 242 207 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5287 5661 5807 387 Size: 1064 138 3698 374 146 1280 Fragments arranged by size: 3698 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3874 3874 Size: 6700 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2040 2109 2260 3243 3344 3500 3677 Size: 393 408 69 151 983 101 156 177 Cuts at: 3677 3704 3809 3871 3971 4763 5360 6147 6415 Size: 27 105 62 100 792 597 787 268 Cuts at: 6415 1239 Size: 1524 Fragments arranged by size: 1524 983 792 787 597 408 393 268 177 156 151 105 101 100 69 62 27 EcoNI CCTnn'n_nnAGG Cuts at: 2759 2990 3584 2759 Size: 231 594 5875 Fragments arranged by size: 5875 594 231 EcoO109I rG'GnC_Cy Cuts at: 2509 2866 2944 3155 3215 3375 3376 3439 3901 Size: 357 78 211 60 160 1 63 462 Cuts at: 3901 2509 Size: 5308 Fragments arranged by size: 5308 462 357 211 160 78 63 60 1 EcoRI G'AATT_C Cuts at: 4009 4387 4009 Size: 378 6322 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3200 3200 Size: 6700 FseI GG_CCGG'CC Cuts at: 2503 2503 Size: 6700 FspI TGC'GCA Cuts at: 5206 5206 Size: 6700 HaeII r_GCGC'y Cuts at: 1678 2731 3331 3772 3988 3996 5847 1678 Size: 1053 600 441 216 8 1851 2531 Fragments arranged by size: 2531 1851 1053 600 441 216 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2204 2236 2614 2646 3056 Size: 32 135 32 632 32 378 32 410 Cuts at: 3056 3088 5341 5373 5415 5447 1373 Size: 32 2253 32 42 32 2626 Fragments arranged by size: 2626 2253 632 410 378 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4044 4290 669 Size: 988 281 2106 246 3079 Fragments arranged by size: 3079 2106 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6700 HpaI GTT'AAC Cuts at: 4290 4290 Size: 6700 KasI G'GCGC_C Cuts at: 3768 3992 3768 Size: 224 6476 KpnI G_GTAC'C Cuts at: 4025 4025 Size: 6700 MfeI C'AATT_G Cuts at: 4299 4299 Size: 6700 MluI A'CGCG_T Cuts at: 3910 6576 3910 Size: 2666 4034 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5814 5998 1305 Size: 4509 184 2007 Fragments arranged by size: 4509 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4878 Fragments arranged by size: 4878 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4658 5017 5176 518 Size: 648 3492 359 159 2042 Fragments arranged by size: 3492 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2012 2060 2177 2217 2449 2773 4116 4793 Size: 406 48 117 40 232 324 1343 677 Cuts at: 4793 5941 6186 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1343 1148 677 406 324 245 232 117 48 40 NaeI GCC'GGC Cuts at: 2501 3979 2501 Size: 1478 5222 NarI GG'CG_CC Cuts at: 3769 3993 3769 Size: 224 6476 NcoI C'CATG_G Cuts at: 513 1065 2004 2504 3249 513 Size: 552 939 500 745 3964 Fragments arranged by size: 3964 939 745 552 500 NdeI CA'TA_TG Cuts at: 387 387 Size: 6700 NgoMIV G'CCGG_C Cuts at: 2499 3977 2499 Size: 1478 5222 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6700 NotI GC'GGCC_GC Cuts at: 4147 4147 Size: 6700 NspI r_CATG'y Cuts at: 1132 1229 4058 5603 1132 Size: 97 2829 1545 2229 Fragments arranged by size: 2829 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3361 1348 Size: 2013 4687 PciI A'CATG_T Cuts at: 5599 5599 Size: 6700 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6700 PfoI T'CCnGG_A Cuts at: 2565 2565 Size: 6700 PmeI GTTT'AAAC Cuts at: 4004 4004 Size: 6700 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3785 3817 4769 4801 6282 6314 3785 Size: 32 952 32 1481 32 4171 Fragments arranged by size: 4171 1481 952 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3155 3439 3901 3155 Size: 284 462 5954 Fragments arranged by size: 5954 462 284 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6700 PsiI TTA'TAA Cuts at: 4270 4270 Size: 6700 PspOMI G'GGCC_C Cuts at: 2508 3375 2508 Size: 867 5833 PstI C_TGCA'G Cuts at: 830 2472 3141 4052 830 Size: 1642 669 911 3478 Fragments arranged by size: 3478 1642 911 669 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6700 PvuII CAG'CTG Cuts at: 2217 2217 Size: 6700 SacI G_AGCT'C Cuts at: 3876 3876 Size: 6700 SacII CC_GC'GG Cuts at: 2013 2450 2013 Size: 437 6263 SalI G'TCGA_C Cuts at: 1936 4042 1936 Size: 2106 4594 SbfI CC_TGCA'GG Cuts at: 4052 4052 Size: 6700 ScaI AGT'ACT Cuts at: 2299 4948 6660 2299 Size: 2649 1712 2339 Fragments arranged by size: 2649 2339 1712 SexAI A'CCwGG_T Cuts at: 2586 2586 Size: 6700 SfcI C'TryA_G Cuts at: 826 1045 2468 2703 3137 3290 3605 3737 4048 Size: 219 1423 235 434 153 315 132 311 Cuts at: 4048 5183 5864 6055 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1423 1135 681 434 315 311 235 219 191 153 132 SfoI GGC'GCC Cuts at: 3770 3994 3770 Size: 224 6476 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6700 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6700 SmaI CCC'GGG Cuts at: 1872 4027 1872 Size: 2155 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3458 4804 5705 5967 820 Size: 197 926 1515 1346 901 262 1553 Fragments arranged by size: 1553 1515 1346 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4491 493 Size: 3998 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6700 SphI G_CATG'C Cuts at: 1132 4058 1132 Size: 2926 3774 SspI AAT'ATT Cuts at: 5 52 4624 5 Size: 47 4572 2081 Fragments arranged by size: 4572 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6700 StyI C'CwwG_G Cuts at: 513 1065 2004 2504 3099 3150 3249 4139 513 Size: 552 939 500 595 51 99 890 3074 Fragments arranged by size: 3074 939 890 595 552 500 99 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2993 4727 4744 4897 5082 5527 325 Size: 1255 1413 1734 17 153 185 445 1498 Fragments arranged by size: 1734 1498 1413 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2297 4946 6658 Size: 274 80 33 51 1443 320 2649 1712 Cuts at: 6658 96 Size: 138 Fragments arranged by size: 2649 1712 1443 320 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6660 6692 6660 Size: 32 6668 Tth111I GACn'n_nGTC Cuts at: 1316 3068 1316 Size: 1752 4948 XbaI T'CTAG_A Cuts at: 2224 4036 2224 Size: 1812 4888 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6700 XmaI C'CCGG_G Cuts at: 1870 4025 1870 Size: 2155 4545 XmnI GAAnn'nnTTC Cuts at: 4829 4829 Size: 6700 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4508 276 Size: 53 83 186 936 2974 2468 Fragments arranged by size: 2974 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI XcmI