Restriction map for
pFN21AE4553
(Circular) (Six-base) MAPSORT of: pFN21AE4553.seq Check: 1577 from: 1 to: 6700
pFN21AE4553 6700 bp
With 182 enzymes: SgfI *
March 28, 2012 14:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4510 278
Size: 53 83 186 936 2974 2468
Fragments arranged by size:
2974 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4043 1937
Size: 2106 4594
Acc65I G'GTAC_C
Cuts at: 4021 4021
Size: 6700
AclI AA'CG_TT
Cuts at: 1563 2779 4827 5200 1563
Size: 1216 2048 373 3063
Fragments arranged by size:
3063 2048 1216 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2040 3677 3809 3871 3971 4763 6147 6415
Size: 408 1637 132 62 100 792 1384 268
Cuts at: 6415 1632
Size: 1917
Fragments arranged by size:
1917 1637 1384 792 408 268 132 100
62
AfeI AGC'GCT
Cuts at: 2729 3986 2729
Size: 1257 5443
AflII C'TTAA_G
Cuts at: 820 1017 3458 820
Size: 197 2441 4062
Fragments arranged by size:
4062 2441 197
AflIII A'CryG_T
Cuts at: 1203 3910 5599 6576 1203
Size: 2707 1689 977 1327
Fragments arranged by size:
2707 1689 1327 977
AgeI A'CCGG_T
Cuts at: 5531 5531
Size: 6700
AhdI GACnn_n'nnGTC
Cuts at: 5429 5429
Size: 6700
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6700
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3162 1184
Size: 1978 4722
AlwNI CAG_nnn'CTG
Cuts at: 2078 2396 3266 4443 6015 2078
Size: 318 870 1177 1572 2763
Fragments arranged by size:
2763 1572 1177 870 318
ApaI G_GGCC'C
Cuts at: 2512 3379 2512
Size: 867 5833
ApaLI G'TGCA_C
Cuts at: 4757 5913 4757
Size: 1156 5544
ApoI r'AATT_y
Cuts at: 1493 4009 4235 4387 6496 1493
Size: 2516 226 152 2109 1697
Fragments arranged by size:
2516 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5254 160
Size: 872 4222 1606
Fragments arranged by size:
4222 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6700
AvaI C'yCGr_G
Cuts at: 1870 1943 3210 3807 4025 1870
Size: 73 1267 597 218 4545
Fragments arranged by size:
4545 1267 597 218 73
BamHI G'GATC_C
Cuts at: 4030 4397 4030
Size: 367 6333
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2489 3768 3992 4021 5476
Size: 325 217 627 702 1279 224 29 1455
Cuts at: 5476 618
Size: 1842
Fragments arranged by size:
1842 1455 1279 702 627 325 224 217
29
BanII G_rGCy'C
Cuts at: 1391 2455 2512 3211 3242 3249 3379 3876 4019
Size: 1064 57 699 31 7 130 497 143
Cuts at: 4019 1391
Size: 4072
Fragments arranged by size:
4072 1064 699 497 143 130 57 31
7
BbeI G_GCGC'C
Cuts at: 3772 3996 3772
Size: 224 6476
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2408 2607 3428 3815 928
Size: 971 509 199 821 387 3813
Fragments arranged by size:
3813 971 821 509 387 199
BbvCI CC'TCA_GC
Cuts at: 3335 3507 3720 3335
Size: 172 213 6315
Fragments arranged by size:
6315 213 172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2586 2620 4891 4925 1903
Size: 34 649 34 2271 34 3678
Fragments arranged by size:
3678 2271 649 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3032 4592 5802 3032
Size: 1560 1210 3930
Fragments arranged by size:
3930 1560 1210
BclI T'GATC_A
Cuts at: 1548 3108 1548
Size: 1560 5140
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3503 5311 136
Size: 107 122 71 631 605 1831 1808 1525
Fragments arranged by size:
1831 1808 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2538 3811 6695 2538
Size: 1273 2884 2543
Fragments arranged by size:
2884 2543 1273
BlpI GC'TnA_GC
Cuts at: 2093 3010 3299 3802 4117 2093
Size: 917 289 503 315 4676
Fragments arranged by size:
4676 917 503 315 289
Bme1580I G_kGCm'C
Cuts at: 1790 2512 3379 4761 5917 1790
Size: 722 867 1382 1156 2573
Fragments arranged by size:
2573 1382 1156 867 722
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6700
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4431 5389 442
Size: 416 53 83 399 24 3014 958 1753
Fragments arranged by size:
3014 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6700
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2316 2316
Size: 6700
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2109 2260 3243 3344 3500 3704 5360 1239
Size: 870 151 983 101 156 204 1656 2579
Fragments arranged by size:
2579 1656 983 870 204 156 151 101
Bpu10I CC'TnA_GC
Cuts at: 3243 3335 3507 3720 3243
Size: 92 172 213 6223
Fragments arranged by size:
6223 213 172 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4825 5690 5988 4825
Size: 865 298 5537
Fragments arranged by size:
5537 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2577 2636 3974 5363 882
Size: 617 1078 59 1338 1389 2219
Fragments arranged by size:
2219 1389 1338 1078 617 59
BsaAI yAC'GTr
Cuts at: 493 1204 4491 493
Size: 711 3287 2702
Fragments arranged by size:
3287 2702 711
BsaBI GATnn'nnATC
Cuts at: 3650 6694 3650
Size: 3044 3656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3769 3993 4507 4889
Size: 53 83 186 936 2236 224 514 382
Cuts at: 4889 5524 275
Size: 635 1451
Fragments arranged by size:
2236 1451 936 635 514 382 224 186
83 53
BsaWI w'CCGG_w
Cuts at: 5133 5531 5805 5952 6363 5133
Size: 398 274 147 411 5470
Fragments arranged by size:
5470 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2574 2604 2714 2744 3056
Size: 30 165 30 1171 30 110 30 312
Cuts at: 3056 3086 1178
Size: 30 4792
Fragments arranged by size:
4792 1171 312 165 110 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2308 2498 2722 3242 3323 3725 1186
Size: 1122 190 224 520 81 402 4161
Fragments arranged by size:
4161 1122 520 402 224 190 81
BseYI C'CCAG_C
Cuts at: 2396 2416 2831 3729 3849 5903 6399 2396
Size: 20 415 898 120 2054 496 2697
Fragments arranged by size:
2697 2054 898 496 415 120 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2348 1163
Size: 573 612 5515
Fragments arranged by size:
5515 612 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4150 4911 5939 1540
Size: 275 186 2149 761 1028 2301
Fragments arranged by size:
2301 2149 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2754 3876 4761 4846 5917 2754
Size: 1122 885 85 1071 3537
Fragments arranged by size:
3537 1122 1071 885 85
BsmI GAATG_Cn'
Cuts at: 2368 4211 4304 2368
Size: 1843 93 4764
Fragments arranged by size:
4764 1843 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3043 3043
Size: 6700
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2455 2512 2754 3211 3242 3249 3379
Size: 399 665 57 242 457 31 7 130
Cuts at: 3379 3876 4019 4761 4846 5917 1391
Size: 497 143 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 665 497 457 399 242
143 130 85 57 31 7
BspEI T'CCGG_A
Cuts at: 6363 6363
Size: 6700
BspHI T'CATG_A
Cuts at: 4589 6319 4589
Size: 1730 4970
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2309 4055 844
Size: 1465 1746 3489
Fragments arranged by size:
3489 1746 1465
BsrBI CCG'CTC
Cuts at: 1394 1823 2671 3406 4587 1394
Size: 429 848 735 1181 3507
Fragments arranged by size:
3507 1181 848 735 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3036 5195 5369 65
Size: 1183 1788 2159 174 1396
Fragments arranged by size:
2159 1788 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2499 2847 3977 5344 5531 1700
Size: 110 689 348 1130 1367 187 2869
Fragments arranged by size:
2869 1367 1130 689 348 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6700
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6700
BssSI C'ACGA_G
Cuts at: 2952 4760 5772 2952
Size: 1808 1012 3880
Fragments arranged by size:
3880 1808 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2413 2680 794
Size: 1619 267 4814
Fragments arranged by size:
4814 1619 267
BstBI TT'CG_AA
Cuts at: 5504 5504
Size: 6700
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6700
BstXI CCAn_nnnn'nTGG
Cuts at: 3493 3989 3493
Size: 496 6204
BstYI r'GATC_y
Cuts at: 1972 2538 3645 3784 3811 4030 4397 4782 4799
Size: 566 1107 139 27 219 367 385 17
Cuts at: 4799 6251 6337 6349 6695 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1107 566 385 367 346 219
139 86 27 17 12
Bsu36I CC'TnA_GG
Cuts at: 2339 3219 3708 2339
Size: 880 489 5331
Fragments arranged by size:
5331 880 489
BtgI C'CryG_G
Cuts at: 513 1065 2004 2010 2447 2504 3249 3877 513
Size: 552 939 6 437 57 745 628 3336
Fragments arranged by size:
3336 939 745 628 552 437 57 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3539 487
Size: 683 56 2313 3648
Fragments arranged by size:
3648 2313 683 56
BtsI GCAGTG_nn'
Cuts at: 4216 5010 5030 4216
Size: 794 20 5886
Fragments arranged by size:
5886 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6700
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6665
DraI TTT'AAA
Cuts at: 4004 4004
Size: 6700
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5707 809
Size: 507 4391 1802
Fragments arranged by size:
4391 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2156 2501
Size: 54 1425 50 275 18 177 149 345
Cuts at: 2501 3315 3979 4147 5036 6665 8
Size: 814 664 168 889 1629 43
Fragments arranged by size:
1629 1425 889 814 664 345 275 177
168 149 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4147 1537
Size: 275 2335 4090
Fragments arranged by size:
4090 2335 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3219 3426 3668 4630 1353
Size: 1866 207 242 962 3423
Fragments arranged by size:
3423 1866 962 242 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5287 5661 5807 387
Size: 1064 138 3698 374 146 1280
Fragments arranged by size:
3698 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3874 3874
Size: 6700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2040 2109 2260 3243 3344 3500 3677
Size: 393 408 69 151 983 101 156 177
Cuts at: 3677 3704 3809 3871 3971 4763 5360 6147 6415
Size: 27 105 62 100 792 597 787 268
Cuts at: 6415 1239
Size: 1524
Fragments arranged by size:
1524 983 792 787 597 408 393 268
177 156 151 105 101 100 69 62
27
EcoNI CCTnn'n_nnAGG
Cuts at: 2759 2990 3584 2759
Size: 231 594 5875
Fragments arranged by size:
5875 594 231
EcoO109I rG'GnC_Cy
Cuts at: 2509 2866 2944 3155 3215 3375 3376 3439 3901
Size: 357 78 211 60 160 1 63 462
Cuts at: 3901 2509
Size: 5308
Fragments arranged by size:
5308 462 357 211 160 78 63 60
1
EcoRI G'AATT_C
Cuts at: 4009 4387 4009
Size: 378 6322
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3200 3200
Size: 6700
FseI GG_CCGG'CC
Cuts at: 2503 2503
Size: 6700
FspI TGC'GCA
Cuts at: 5206 5206
Size: 6700
HaeII r_GCGC'y
Cuts at: 1678 2731 3331 3772 3988 3996 5847 1678
Size: 1053 600 441 216 8 1851 2531
Fragments arranged by size:
2531 1851 1053 600 441 216 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2204 2236 2614 2646 3056
Size: 32 135 32 632 32 378 32 410
Cuts at: 3056 3088 5341 5373 5415 5447 1373
Size: 32 2253 32 42 32 2626
Fragments arranged by size:
2626 2253 632 410 378 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4044 4290 669
Size: 988 281 2106 246 3079
Fragments arranged by size:
3079 2106 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6700
HpaI GTT'AAC
Cuts at: 4290 4290
Size: 6700
KasI G'GCGC_C
Cuts at: 3768 3992 3768
Size: 224 6476
KpnI G_GTAC'C
Cuts at: 4025 4025
Size: 6700
MfeI C'AATT_G
Cuts at: 4299 4299
Size: 6700
MluI A'CGCG_T
Cuts at: 3910 6576 3910
Size: 2666 4034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5814 5998 1305
Size: 4509 184 2007
Fragments arranged by size:
4509 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4878
Fragments arranged by size:
4878 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4658 5017 5176 518
Size: 648 3492 359 159 2042
Fragments arranged by size:
3492 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2060 2177 2217 2449 2773 4116 4793
Size: 406 48 117 40 232 324 1343 677
Cuts at: 4793 5941 6186 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1343 1148 677 406 324 245 232
117 48 40
NaeI GCC'GGC
Cuts at: 2501 3979 2501
Size: 1478 5222
NarI GG'CG_CC
Cuts at: 3769 3993 3769
Size: 224 6476
NcoI C'CATG_G
Cuts at: 513 1065 2004 2504 3249 513
Size: 552 939 500 745 3964
Fragments arranged by size:
3964 939 745 552 500
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6700
NgoMIV G'CCGG_C
Cuts at: 2499 3977 2499
Size: 1478 5222
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6700
NotI GC'GGCC_GC
Cuts at: 4147 4147
Size: 6700
NspI r_CATG'y
Cuts at: 1132 1229 4058 5603 1132
Size: 97 2829 1545 2229
Fragments arranged by size:
2829 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3361 1348
Size: 2013 4687
PciI A'CATG_T
Cuts at: 5599 5599
Size: 6700
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6700
PfoI T'CCnGG_A
Cuts at: 2565 2565
Size: 6700
PmeI GTTT'AAAC
Cuts at: 4004 4004
Size: 6700
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 4769 4801 6282 6314 3785
Size: 32 952 32 1481 32 4171
Fragments arranged by size:
4171 1481 952 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3155 3439 3901 3155
Size: 284 462 5954
Fragments arranged by size:
5954 462 284
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6700
PsiI TTA'TAA
Cuts at: 4270 4270
Size: 6700
PspOMI G'GGCC_C
Cuts at: 2508 3375 2508
Size: 867 5833
PstI C_TGCA'G
Cuts at: 830 2472 3141 4052 830
Size: 1642 669 911 3478
Fragments arranged by size:
3478 1642 911 669
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6700
PvuII CAG'CTG
Cuts at: 2217 2217
Size: 6700
SacI G_AGCT'C
Cuts at: 3876 3876
Size: 6700
SacII CC_GC'GG
Cuts at: 2013 2450 2013
Size: 437 6263
SalI G'TCGA_C
Cuts at: 1936 4042 1936
Size: 2106 4594
SbfI CC_TGCA'GG
Cuts at: 4052 4052
Size: 6700
ScaI AGT'ACT
Cuts at: 2299 4948 6660 2299
Size: 2649 1712 2339
Fragments arranged by size:
2649 2339 1712
SexAI A'CCwGG_T
Cuts at: 2586 2586
Size: 6700
SfcI C'TryA_G
Cuts at: 826 1045 2468 2703 3137 3290 3605 3737 4048
Size: 219 1423 235 434 153 315 132 311
Cuts at: 4048 5183 5864 6055 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1423 1135 681 434 315 311 235
219 191 153 132
SfoI GGC'GCC
Cuts at: 3770 3994 3770
Size: 224 6476
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6700
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6700
SmaI CCC'GGG
Cuts at: 1872 4027 1872
Size: 2155 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3458 4804 5705 5967 820
Size: 197 926 1515 1346 901 262 1553
Fragments arranged by size:
1553 1515 1346 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4491 493
Size: 3998 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6700
SphI G_CATG'C
Cuts at: 1132 4058 1132
Size: 2926 3774
SspI AAT'ATT
Cuts at: 5 52 4624 5
Size: 47 4572 2081
Fragments arranged by size:
4572 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6700
StyI C'CwwG_G
Cuts at: 513 1065 2004 2504 3099 3150 3249 4139 513
Size: 552 939 500 595 51 99 890 3074
Fragments arranged by size:
3074 939 890 595 552 500 99 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2993 4727 4744 4897 5082 5527 325
Size: 1255 1413 1734 17 153 185 445 1498
Fragments arranged by size:
1734 1498 1413 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2297 4946 6658
Size: 274 80 33 51 1443 320 2649 1712
Cuts at: 6658 96
Size: 138
Fragments arranged by size:
2649 1712 1443 320 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6660 6692 6660
Size: 32 6668
Tth111I GACn'n_nGTC
Cuts at: 1316 3068 1316
Size: 1752 4948
XbaI T'CTAG_A
Cuts at: 2224 4036 2224
Size: 1812 4888
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6700
XmaI C'CCGG_G
Cuts at: 1870 4025 1870
Size: 2155 4545
XmnI GAAnn'nnTTC
Cuts at: 4829 4829
Size: 6700
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4508 276
Size: 53 83 186 936 2974 2468
Fragments arranged by size:
2974 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoRV FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SanDI SapI SfiI SrfI SwaI XcmI