Restriction map for
pFN21AE4545
(Circular) (Six-base) MAPSORT of: pFN21AE4545.seq Check: 8266 from: 1 to: 6610
pFN21AE4545 6610 bp
With 182 enzymes: SgfI *
March 28, 2012 14:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4420 278
Size: 53 83 186 936 2884 2468
Fragments arranged by size:
2884 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2742 3642 3953 1937
Size: 805 900 311 4594
Fragments arranged by size:
4594 900 805 311
Acc65I G'GTAC_C
Cuts at: 3540 3931 3540
Size: 391 6219
AclI AA'CG_TT
Cuts at: 1563 3618 4737 5110 1563
Size: 2055 1119 373 3063
Fragments arranged by size:
3063 2055 1119 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4673 6057 6325 1632
Size: 3041 1384 268 1917
Fragments arranged by size:
3041 1917 1384 268
AfeI AGC'GCT
Cuts at: 3773 3773
Size: 6610
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6413
AflIII A'CryG_T
Cuts at: 1203 2226 2516 3657 5509 6486 1203
Size: 1023 290 1141 1852 977 1327
Fragments arranged by size:
1852 1327 1141 1023 977 290
AgeI A'CCGG_T
Cuts at: 5441 5441
Size: 6610
AhdI GACnn_n'nnGTC
Cuts at: 2623 2715 5339 2623
Size: 92 2624 3894
Fragments arranged by size:
3894 2624 92
AleI CACnn'nnGTG
Cuts at: 1166 3656 1166
Size: 2490 4120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3467 1184
Size: 2283 4327
AlwNI CAG_nnn'CTG
Cuts at: 3784 4353 5925 3784
Size: 569 1572 4469
Fragments arranged by size:
4469 1572 569
ApaI G_GGCC'C
Cuts at: 2066 2085 2095 2695 2974 2066
Size: 19 10 600 279 5702
Fragments arranged by size:
5702 600 279 19 10
ApaLI G'TGCA_C
Cuts at: 3119 4667 5823 3119
Size: 1548 1156 3906
Fragments arranged by size:
3906 1548 1156
ApoI r'AATT_y
Cuts at: 1493 3919 4145 4297 6406 1493
Size: 2426 226 152 2109 1697
Fragments arranged by size:
2426 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5164 160
Size: 872 4132 1606
Fragments arranged by size:
4132 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6610
AvaI C'yCGr_G
Cuts at: 1870 1943 2078 3935 1870
Size: 73 135 1857 4545
Fragments arranged by size:
4545 1857 135 73
BamHI G'GATC_C
Cuts at: 3940 4307 3940
Size: 367 6243
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2683 2855 3443 3540 3758
Size: 325 217 627 896 172 588 97 218
Cuts at: 3758 3931 5386 618
Size: 173 1455 1842
Fragments arranged by size:
1842 1455 896 627 588 325 218 217
173 172 97
BanII G_rGCy'C
Cuts at: 1391 2066 2085 2095 2695 2974 3829 3929 1391
Size: 675 19 10 600 279 855 100 4072
Fragments arranged by size:
4072 855 675 600 279 100 19 10
BbeI G_GCGC'C
Cuts at: 2859 2859
Size: 6610
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2419 2574 3073 3403 928
Size: 971 520 155 499 330 4135
Fragments arranged by size:
4135 971 520 499 330 155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3868 3871 3902 3905 4801 4835 1903
Size: 34 1931 3 31 3 896 34 3678
Fragments arranged by size:
3678 1931 896 34 34 31 3 3
BciVI GTATCCnnnnn_n'
Cuts at: 4502 5712 4502
Size: 1210 5400
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6610
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2118 2588 5221
Size: 107 122 71 631 605 446 470 2633
Cuts at: 5221 136
Size: 1525
Fragments arranged by size:
2633 1525 631 605 470 446 122 107
71
BglII A'GATC_T
Cuts at: 2331 2553 3336 6605 2331
Size: 222 783 3269 2336
Fragments arranged by size:
3269 2336 783 222
BlpI GC'TnA_GC
Cuts at: 3768 4027 3768
Size: 259 6351
Bme1580I G_kGCm'C
Cuts at: 1790 2066 2085 2095 2695 2974 3123 3361 3761
Size: 276 19 10 600 279 149 238 400
Cuts at: 3761 4671 5827 1790
Size: 910 1156 2573
Fragments arranged by size:
2573 1156 910 600 400 279 276 238
149 19 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6610
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2710 3860 4341
Size: 416 53 83 399 24 1293 1150 481
Cuts at: 4341 5299 442
Size: 958 1753
Fragments arranged by size:
1753 1293 1150 958 481 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2699 5270 1239
Size: 1460 2571 2579
Fragments arranged by size:
2579 2571 1460
Bpu10I CC'TnA_GC
Cuts at: 3828 3828
Size: 6610
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2680 2867 4735 5600 5898 2680
Size: 187 1868 865 298 3392
Fragments arranged by size:
3392 1868 865 298 187
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2156 3251 5273 882
Size: 617 657 1095 2022 2219
Fragments arranged by size:
2219 2022 1095 657 617
BsaAI yAC'GTr
Cuts at: 493 1204 2812 3658 4401 493
Size: 711 1608 846 743 2702
Fragments arranged by size:
2702 1608 846 743 711
BsaBI GATnn'nnATC
Cuts at: 6604 6604
Size: 6610
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2856 3024 4417 4799
Size: 53 83 186 936 1323 168 1393 382
Cuts at: 4799 5434 275
Size: 635 1451
Fragments arranged by size:
1451 1393 1323 936 635 382 186 168
83 53
BsaWI w'CCGG_w
Cuts at: 5043 5441 5715 5862 6273 5043
Size: 398 274 147 411 5380
Fragments arranged by size:
5380 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6385
Fragments arranged by size:
6385 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3281 3290 3734 1186
Size: 2095 9 444 4062
Fragments arranged by size:
4062 2095 444 9
BseYI C'CCAG_C
Cuts at: 2338 2730 2902 3687 3789 5813 6309 2338
Size: 392 172 785 102 2024 496 2639
Fragments arranged by size:
2639 2024 785 496 392 172 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3710 1163
Size: 573 1974 4063
Fragments arranged by size:
4063 1974 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2140 4060 4821 5849 1540
Size: 275 186 139 1920 761 1028 2301
Fragments arranged by size:
2301 1920 1028 761 275 186 139
BsiHKAI G_wGCw'C
Cuts at: 3123 3829 4671 4756 5827 3123
Size: 706 842 85 1071 3906
Fragments arranged by size:
3906 1071 842 706 85
BsmI GAATG_Cn'
Cuts at: 4121 4214 4121
Size: 93 6517
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2085 2095 2695 2974 3123 3361
Size: 399 276 19 10 600 279 149 238
Cuts at: 3361 3761 3829 3929 4671 4756 5827 1391
Size: 400 68 100 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 600 400 399 279 276
238 149 100 85 68 19 10
BspEI T'CCGG_A
Cuts at: 6273 6273
Size: 6610
BspHI T'CATG_A
Cuts at: 2349 4499 6229 2349
Size: 2150 1730 2730
Fragments arranged by size:
2730 2150 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3965 844
Size: 3121 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4497 1394
Size: 429 2674 3507
Fragments arranged by size:
3507 2674 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3522 5105 5279 65
Size: 1183 2274 1583 174 1396
Fragments arranged by size:
2274 1583 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2357 2622 3284 5254 5441 1700
Size: 110 547 265 662 1970 187 2869
Fragments arranged by size:
2869 1970 662 547 265 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6610
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6610
BssSI C'ACGA_G
Cuts at: 4670 5682 4670
Size: 1012 5598
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3118 794
Size: 2324 4286
BstBI TT'CG_AA
Cuts at: 5414 5414
Size: 6610
BstEII G'GTnAC_C
Cuts at: 1184 2707 1184
Size: 1523 5087
BstXI CCAn_nnnn'nTGG
Cuts at: 2965 3034 2965
Size: 69 6541
BstYI r'GATC_y
Cuts at: 1972 2331 2553 3336 3940 4307 4692 4709 6161
Size: 359 222 783 604 367 385 17 1452
Cuts at: 6161 6247 6259 6605 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 783 604 385 367 359 346
222 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2913 3746 2913
Size: 833 5777
BtgI C'CryG_G
Cuts at: 513 1065 2004 2115 2211 2415 2772 3173 3384
Size: 552 939 111 96 204 357 401 211
Cuts at: 3384 3665 3681 3762 513
Size: 281 16 81 3361
Fragments arranged by size:
3361 939 552 401 357 281 211 204
111 96 81 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2056 3148 487
Size: 683 56 830 1092 3949
Fragments arranged by size:
3949 1092 830 683 56
BtsI GCAGTG_nn'
Cuts at: 2793 4126 4920 4940 2793
Size: 1333 794 20 4463
Fragments arranged by size:
4463 1333 794 20
ClaI AT'CG_AT
Cuts at: 1554 3375 1554
Size: 1821 4789
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6575
DraI TTT'AAA
Cuts at: 3914 3914
Size: 6610
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3839 3868 5617 809
Size: 507 2523 29 1749 1802
Fragments arranged by size:
2523 1802 1749 507 29
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 2137 2145
Size: 54 1425 50 275 18 267 40 8
Cuts at: 2145 2521 2589 3435 4057 4946 6575 8
Size: 376 68 846 622 889 1629 43
Fragments arranged by size:
1629 1425 889 846 622 376 275 267
68 54 50 43 40 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 2137 4057 1537
Size: 275 325 1920 4090
Fragments arranged by size:
4090 1920 325 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2009 4540 1353
Size: 656 2531 3423
Fragments arranged by size:
3423 2531 656
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2277 2549 5197 5571 5717 387
Size: 1064 138 688 272 2648 374 146 1280
Fragments arranged by size:
2648 1280 1064 688 374 272 146 138
EcoICRI GAG'CTC
Cuts at: 3827 3827
Size: 6610
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2699 4673 5270 6057 6325 1239
Size: 393 1067 1974 597 787 268 1524
Fragments arranged by size:
1974 1524 1067 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2062 2063 2091 2092 2691 2692 2907 2934 2970
Size: 1 28 1 599 1 215 27 36
Cuts at: 2970 2971 3802 3847 2062
Size: 1 831 45 4825
Fragments arranged by size:
4825 831 599 215 45 36 28 27
1 1 1 1
EcoRI G'AATT_C
Cuts at: 3919 4297 3919
Size: 378 6232
FspI TGC'GCA
Cuts at: 5116 5116
Size: 6610
HaeII r_GCGC'y
Cuts at: 1678 2022 2236 2859 3775 5757 1678
Size: 344 214 623 916 1982 2531
Fragments arranged by size:
2531 1982 916 623 344 214
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2609 2641 2701 2733 3416
Size: 32 135 32 1037 32 60 32 683
Cuts at: 3416 3448 5251 5283 5325 5357 1373
Size: 32 1803 32 42 32 2626
Fragments arranged by size:
2626 1803 1037 683 135 60 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3954 4200 669
Size: 988 281 2016 246 3079
Fragments arranged by size:
3079 2016 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6610
HpaI GTT'AAC
Cuts at: 4200 4200
Size: 6610
KasI G'GCGC_C
Cuts at: 2855 2855
Size: 6610
KpnI G_GTAC'C
Cuts at: 3544 3935 3544
Size: 391 6219
MfeI C'AATT_G
Cuts at: 4209 4209
Size: 6610
MluI A'CGCG_T
Cuts at: 6486 6486
Size: 6610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2284 5724 5908 1305
Size: 979 3440 184 2007
Fragments arranged by size:
3440 2007 979 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2523 2591 10
Size: 54 1425 343 691 68 4029
Fragments arranged by size:
4029 1425 691 343 68 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2453 3188 3656 4568 4927 5086 518
Size: 648 1287 735 468 912 359 159 2042
Fragments arranged by size:
2042 1287 912 735 648 468 359 159
MspA1I CmG'CkG
Cuts at: 1606 3175 3418 3441 3589 3637 3724 4026 4703
Size: 1569 243 23 148 48 87 302 677
Cuts at: 4703 5851 6096 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1569 1148 677 302 245 243 148
87 48 23
NaeI GCC'GGC
Cuts at: 2359 2359
Size: 6610
NarI GG'CG_CC
Cuts at: 2856 2856
Size: 6610
NcoI C'CATG_G
Cuts at: 513 1065 2004 2415 3384 3681 3762 513
Size: 552 939 411 969 297 81 3361
Fragments arranged by size:
3361 969 939 552 411 297 81
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6610
NgoMIV G'CCGG_C
Cuts at: 2357 2357
Size: 6610
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6610
NotI GC'GGCC_GC
Cuts at: 2137 4057 2137
Size: 1920 4690
NspI r_CATG'y
Cuts at: 1132 1229 2520 3968 5513 1132
Size: 97 1291 1448 1545 2229
Fragments arranged by size:
2229 1545 1448 1291 97
PasI CC'CwG_GG
Cuts at: 1348 2508 2983 1348
Size: 1160 475 4975
Fragments arranged by size:
4975 1160 475
PciI A'CATG_T
Cuts at: 2516 5509 2516
Size: 2993 3617
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3687 3814 1108
Size: 2579 127 3904
Fragments arranged by size:
3904 2579 127
PmeI GTTT'AAAC
Cuts at: 3914 3914
Size: 6610
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4679 4711 6192 6224 4679
Size: 32 1481 32 5065
Fragments arranged by size:
5065 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2907 2934 2907
Size: 27 6583
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6610
PsiI TTA'TAA
Cuts at: 4180 4180
Size: 6610
PspOMI G'GGCC_C
Cuts at: 2062 2081 2091 2691 2970 2062
Size: 19 10 600 279 5702
Fragments arranged by size:
5702 600 279 19 10
PstI C_TGCA'G
Cuts at: 830 3109 3962 830
Size: 2279 853 3478
Fragments arranged by size:
3478 2279 853
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6610
PvuII CAG'CTG
Cuts at: 3418 3418
Size: 6610
SacI G_AGCT'C
Cuts at: 3829 3829
Size: 6610
SacII CC_GC'GG
Cuts at: 3176 3176
Size: 6610
SalI G'TCGA_C
Cuts at: 1936 3952 1936
Size: 2016 4594
SanDI GG'GwC_CC
Cuts at: 2907 2934 2907
Size: 27 6583
SapI GCTCTTCn'nnn_
Cuts at: 2009 2009
Size: 6610
SbfI CC_TGCA'GG
Cuts at: 3962 3962
Size: 6610
ScaI AGT'ACT
Cuts at: 4858 6570 4858
Size: 1712 4898
SexAI A'CCwGG_T
Cuts at: 2478 2478
Size: 6610
SfcI C'TryA_G
Cuts at: 826 1045 2035 3105 3958 5093 5774 5965 826
Size: 219 990 1070 853 1135 681 191 1471
Fragments arranged by size:
1471 1135 1070 990 853 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2118 2118
Size: 6610
SfoI GGC'GCC
Cuts at: 2857 2857
Size: 6610
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6610
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6610
SmaI CCC'GGG
Cuts at: 1872 2080 3937 1872
Size: 208 1857 4545
Fragments arranged by size:
4545 1857 208
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2659 2882 4714 5615 5877 820
Size: 197 926 716 223 1832 901 262 1553
Fragments arranged by size:
1832 1553 926 901 716 262 223 197
SnaBI TAC'GTA
Cuts at: 493 4401 493
Size: 3908 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6610
SphI G_CATG'C
Cuts at: 1132 3968 1132
Size: 2836 3774
SrfI GCCC'GGGC
Cuts at: 2080 2080
Size: 6610
SspI AAT'ATT
Cuts at: 5 52 4534 5
Size: 47 4482 2081
Fragments arranged by size:
4482 2081 47
StuI AGG'CCT
Cuts at: 1517 2400 3464 1517
Size: 883 1064 4663
Fragments arranged by size:
4663 1064 883
StyI C'CwwG_G
Cuts at: 513 1065 2004 2100 2415 3129 3384 3681 3753
Size: 552 939 96 315 714 255 297 72
Cuts at: 3753 3762 4049 513
Size: 9 287 3074
Fragments arranged by size:
3074 939 714 552 315 297 287 255
96 72 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2722 3481 4637 4654 4807 4992 5437
Size: 1255 1142 759 1156 17 153 185 445
Cuts at: 5437 325
Size: 1498
Fragments arranged by size:
1498 1255 1156 1142 759 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2787 3624 4856
Size: 274 80 33 51 1443 810 837 1232
Cuts at: 4856 6568 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1232 837 810 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2667 2699 2769 2801 6570 6602 2667
Size: 32 70 32 3769 32 2675
Fragments arranged by size:
3769 2675 70 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6610
XbaI T'CTAG_A
Cuts at: 3946 3946
Size: 6610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2584 3760 2584
Size: 1176 5434
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6610
XmaI C'CCGG_G
Cuts at: 1870 2078 3935 1870
Size: 208 1857 4545
Fragments arranged by size:
4545 1857 208
XmnI GAAnn'nnTTC
Cuts at: 4739 4739
Size: 6610
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4418 276
Size: 53 83 186 936 2884 2468
Fragments arranged by size:
2884 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SwaI