Restriction map for
pFN21AE4541
(Circular) (Six-base) MAPSORT of: pFN21AE4541.seq Check: 7944 from: 1 to: 6574
pFN21AE4541 6574 bp
With 182 enzymes: SgfI *
March 28, 2012 14:11 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4384 278
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3917 1937
Size: 1980 4594
Acc65I G'GTAC_C
Cuts at: 3778 3895 3778
Size: 117 6457
AclI AA'CG_TT
Cuts at: 1563 4701 5074 1563
Size: 3138 373 3063
Fragments arranged by size:
3138 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2286 3512 3590 3830 4637 6021 6289 1632
Size: 654 1226 78 240 807 1384 268 1917
Fragments arranged by size:
1917 1384 1226 807 654 268 240 78
AfeI AGC'GCT
Cuts at: 3649 3649
Size: 6574
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6377
AflIII A'CryG_T
Cuts at: 1203 5473 6450 1203
Size: 4270 977 1327
Fragments arranged by size:
4270 1327 977
AgeI A'CCGG_T
Cuts at: 5405 5405
Size: 6574
AhdI GACnn_n'nnGTC
Cuts at: 5303 5303
Size: 6574
AleI CACnn'nnGTG
Cuts at: 1166 2360 1166
Size: 1194 5380
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6574
AlwNI CAG_nnn'CTG
Cuts at: 2038 2977 4317 5889 2038
Size: 939 1340 1572 2723
Fragments arranged by size:
2723 1572 1340 939
ApaI G_GGCC'C
Cuts at: 3282 3282
Size: 6574
ApaLI G'TGCA_C
Cuts at: 4631 5787 4631
Size: 1156 5418
ApoI r'AATT_y
Cuts at: 1493 3034 3883 4109 4261 6370 1493
Size: 1541 849 226 152 2109 1697
Fragments arranged by size:
2109 1697 1541 849 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5128 160
Size: 872 4096 1606
Fragments arranged by size:
4096 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
AvaI C'yCGr_G
Cuts at: 1870 1943 2390 3899 1870
Size: 73 447 1509 4545
Fragments arranged by size:
4545 1509 447 73
BamHI G'GATC_C
Cuts at: 3669 3904 4271 3669
Size: 235 367 5972
Fragments arranged by size:
5972 367 235
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2331 3151 3778 3895 5350
Size: 325 217 627 544 820 627 117 1455
Cuts at: 5350 618
Size: 1842
Fragments arranged by size:
1842 1455 820 627 627 544 325 217
117
BanII G_rGCy'C
Cuts at: 1391 2108 2489 3282 3893 1391
Size: 717 381 793 611 4072
Fragments arranged by size:
4072 793 717 611 381
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3279 928
Size: 971 1380 4223
Fragments arranged by size:
4223 1380 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2410 2436 2444 2470 3815 3849 4765
Size: 34 473 26 8 26 1345 34 916
Cuts at: 4765 4799 1903
Size: 34 3678
Fragments arranged by size:
3678 1345 916 473 34 34 34 26
26 8
BciVI GTATCCnnnnn_n'
Cuts at: 4466 5676 4466
Size: 1210 5364
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6574
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5185 136
Size: 107 122 71 631 605 3513 1525
Fragments arranged by size:
3513 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2823 3051 3301 6569 2823
Size: 228 250 3268 2828
Fragments arranged by size:
3268 2828 250 228
BlpI GC'TnA_GC
Cuts at: 2805 3801 3991 2805
Size: 996 190 5388
Fragments arranged by size:
5388 996 190
Bme1580I G_kGCm'C
Cuts at: 1790 2334 3156 3282 4635 5791 1790
Size: 544 822 126 1353 1156 2573
Fragments arranged by size:
2573 1353 1156 822 544 126
BmgBI CAC'GTC
Cuts at: 1318 2121 2362 1318
Size: 803 241 5530
Fragments arranged by size:
5530 803 241
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4305 5263 442
Size: 416 53 83 399 24 2888 958 1753
Fragments arranged by size:
2888 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5234 1239
Size: 3995 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4699 5564 5862 4699
Size: 865 298 5411
Fragments arranged by size:
5411 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5237 882
Size: 617 3738 2219
Fragments arranged by size:
3738 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2072 4365 493
Size: 711 868 2293 2702
Fragments arranged by size:
2702 2293 868 711
BsaBI GATnn'nnATC
Cuts at: 2603 3587 6568 2603
Size: 984 2981 2609
Fragments arranged by size:
2981 2609 984
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4381 4763 5398 275
Size: 53 83 186 936 2848 382 635 1451
Fragments arranged by size:
2848 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3414 5007 5405 5679 5826 6237 3414
Size: 1593 398 274 147 411 3751
Fragments arranged by size:
3751 1593 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2660 2690 3266 3296 1178
Size: 30 165 30 1257 30 576 30 4456
Fragments arranged by size:
4456 1257 576 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2684 1186
Size: 1498 5076
BseYI C'CCAG_C
Cuts at: 2461 2915 2924 2986 3527 5777 6273 2461
Size: 454 9 62 541 2250 496 2762
Fragments arranged by size:
2762 2250 541 496 454 62 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3524 3747 1163
Size: 573 1788 223 3990
Fragments arranged by size:
3990 1788 573 223
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4024 4785 5813 1540
Size: 275 186 2023 761 1028 2301
Fragments arranged by size:
2301 2023 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2223 3069 4635 4720 5791 2223
Size: 846 1566 85 1071 3006
Fragments arranged by size:
3006 1566 1071 846 85
BsmI GAATG_Cn'
Cuts at: 4085 4178 4085
Size: 93 6481
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2223 2334 2489 3069 3156 3282
Size: 399 318 115 111 155 580 87 126
Cuts at: 3282 3893 4635 4720 5791 1391
Size: 611 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 611 580 399 318 155
126 115 111 87 85
BspEI T'CCGG_A
Cuts at: 3414 6237 3414
Size: 2823 3751
BspHI T'CATG_A
Cuts at: 4463 6193 4463
Size: 1730 4844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3929 844
Size: 3085 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4461 1394
Size: 429 2638 3507
Fragments arranged by size:
3507 2638 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2718 2895 3093 3837 5069 5243 65
Size: 1183 1470 177 198 744 1232 174 1396
Fragments arranged by size:
1470 1396 1232 1183 744 198 177 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2417 5218 5405 1700
Size: 110 607 2801 187 2869
Fragments arranged by size:
2869 2801 607 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6574
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6574
BssSI C'ACGA_G
Cuts at: 3542 3651 4634 5646 3542
Size: 109 983 1012 4470
Fragments arranged by size:
4470 1012 983 109
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2038 794
Size: 1244 5330
BstBI TT'CG_AA
Cuts at: 5378 5378
Size: 6574
BstEII G'GTnAC_C
Cuts at: 1184 3511 3532 1184
Size: 2327 21 4226
Fragments arranged by size:
4226 2327 21
BstXI CCAn_nnnn'nTGG
Cuts at: 2650 2800 2650
Size: 150 6424
BstYI r'GATC_y
Cuts at: 1972 2823 2835 2853 3051 3301 3669 3904 4271
Size: 851 12 18 198 250 368 235 367
Cuts at: 4271 4656 4673 6125 6211 6223 6569 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 851 385 368 367 346 250
235 198 86 18 17 12 12
Bsu36I CC'TnA_GG
Cuts at: 2303 2303
Size: 6574
BtgI C'CryG_G
Cuts at: 513 1065 2004 2502 3678 513
Size: 552 939 498 1176 3409
Fragments arranged by size:
3409 1176 939 552 498
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2995 3103 487
Size: 683 56 1769 108 3958
Fragments arranged by size:
3958 1769 683 108 56
BtsI GCAGTG_nn'
Cuts at: 2094 3250 3381 4090 4884 4904 2094
Size: 1156 131 709 794 20 3764
Fragments arranged by size:
3764 1156 794 709 131 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6574
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6539
DraI TTT'AAA
Cuts at: 3878 3878
Size: 6574
DraIII CAC_nnn'GTG
Cuts at: 2665 2665
Size: 6574
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3467 5581 809
Size: 507 2151 2114 1802
Fragments arranged by size:
2151 2114 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2110 2280
Size: 54 1425 50 275 18 228 52 170
Cuts at: 2280 2364 2541 2689 2979 3210 3552 4021 4910
Size: 84 177 148 290 231 342 469 889
Cuts at: 4910 6539 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 469 342 290 275 231
228 177 170 148 84 54 52 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4021 1537
Size: 275 2209 4090
Fragments arranged by size:
4090 2209 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3488 4504 1353
Size: 2135 1016 3423
Fragments arranged by size:
3423 2135 1016
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2303 5161 5535 5681 387
Size: 1064 138 714 2858 374 146 1280
Fragments arranged by size:
2858 1280 1064 714 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2286 3512 3590 3830 4637 5234 6021
Size: 393 654 1226 78 240 807 597 787
Cuts at: 6021 6289 1239
Size: 268 1524
Fragments arranged by size:
1524 1226 807 787 654 597 393 268
240 78
EcoNI CCTnn'n_nnAGG
Cuts at: 2282 2381 3542 2282
Size: 99 1161 5314
Fragments arranged by size:
5314 1161 99
EcoO109I rG'GnC_Cy
Cuts at: 2188 2386 3279 3856 2188
Size: 198 893 577 4906
Fragments arranged by size:
4906 893 577 198
EcoRI G'AATT_C
Cuts at: 3883 4261 3883
Size: 378 6196
FspI TGC'GCA
Cuts at: 5080 5080
Size: 6574
HaeII r_GCGC'y
Cuts at: 1678 2136 3651 5721 1678
Size: 458 1515 2070 2531
Fragments arranged by size:
2531 2070 1515 458
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2753 2785 2870 2902 3266
Size: 32 135 32 1181 32 85 32 364
Cuts at: 3266 3298 3704 3736 5215 5247 5289 5321 1373
Size: 32 406 32 1479 32 42 32 2626
Fragments arranged by size:
2626 1479 1181 406 364 135 85 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3772 3918 4164 669
Size: 988 281 1834 146 246 3079
Fragments arranged by size:
3079 1834 988 281 246 146
HindIII A'AGCT_T
Cuts at: 748 2349 748
Size: 1601 4973
HpaI GTT'AAC
Cuts at: 3772 4164 3772
Size: 392 6182
KpnI G_GTAC'C
Cuts at: 3782 3899 3782
Size: 117 6457
MfeI C'AATT_G
Cuts at: 4173 4173
Size: 6574
MluI A'CGCG_T
Cuts at: 6450 6450
Size: 6574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2343 3451 5688 5872 1305
Size: 1038 1108 2237 184 2007
Fragments arranged by size:
2237 2007 1108 1038 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2112 2366 2543 2691 2981
Size: 54 1425 343 280 254 177 148 290
Cuts at: 2981 10
Size: 3603
Fragments arranged by size:
3603 1425 343 290 280 254 177 148
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2360 2648 2909 2997 3122 3593 3693
Size: 648 1194 288 261 88 125 471 100
Cuts at: 3693 4532 4891 5050 518
Size: 839 359 159 2042
Fragments arranged by size:
2042 1194 839 648 471 359 288 261
159 125 100 88
MspA1I CmG'CkG
Cuts at: 1606 2587 3557 3800 3990 4667 5815 6060 1606
Size: 981 970 243 190 677 1148 245 2120
Fragments arranged by size:
2120 1148 981 970 677 245 243 190
NcoI C'CATG_G
Cuts at: 513 1065 2004 2502 513
Size: 552 939 498 4585
Fragments arranged by size:
4585 939 552 498
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6574
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6574
NotI GC'GGCC_GC
Cuts at: 4021 4021
Size: 6574
NspI r_CATG'y
Cuts at: 1132 1229 2642 2914 3932 5477 1132
Size: 97 1413 272 1018 1545 2229
Fragments arranged by size:
2229 1545 1413 1018 272 97
PasI CC'CwG_GG
Cuts at: 1348 2377 1348
Size: 1029 5545
PciI A'CATG_T
Cuts at: 5473 5473
Size: 6574
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3353 3788 1108
Size: 2245 435 3894
Fragments arranged by size:
3894 2245 435
PfoI T'CCnGG_A
Cuts at: 2817 2817
Size: 6574
PmeI GTTT'AAAC
Cuts at: 3878 3878
Size: 6574
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4643 4675 6156 6188 4643
Size: 32 1481 32 5029
Fragments arranged by size:
5029 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2188 2188
Size: 6574
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6574
PsiI TTA'TAA
Cuts at: 4144 4144
Size: 6574
PspOMI G'GGCC_C
Cuts at: 3278 3278
Size: 6574
PstI C_TGCA'G
Cuts at: 830 2043 2132 2592 3164 3926 830
Size: 1213 89 460 572 762 3478
Fragments arranged by size:
3478 1213 762 572 460 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6574
PvuII CAG'CTG
Cuts at: 3800 3800
Size: 6574
RsrII CG'GwC_CG
Cuts at: 3073 3073
Size: 6574
SalI G'TCGA_C
Cuts at: 1936 3916 1936
Size: 1980 4594
SapI GCTCTTCn'nnn_
Cuts at: 3488 3488
Size: 6574
SbfI CC_TGCA'GG
Cuts at: 3926 3926
Size: 6574
ScaI AGT'ACT
Cuts at: 4822 6534 4822
Size: 1712 4862
SexAI A'CCwGG_T
Cuts at: 3507 3507
Size: 6574
SfcI C'TryA_G
Cuts at: 826 1045 2039 2128 2588 2827 3160 3363 3922
Size: 219 994 89 460 239 333 203 559
Cuts at: 3922 5057 5738 5929 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 994 681 559 460 333 239
219 203 191 89
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6574
SmaI CCC'GGG
Cuts at: 1872 3901 1872
Size: 2029 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4678 5579 5841 820
Size: 197 926 2735 901 262 1553
Fragments arranged by size:
2735 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2072 4365 493
Size: 1579 2293 2702
Fragments arranged by size:
2702 2293 1579
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6574
SphI G_CATG'C
Cuts at: 1132 2642 2914 3932 1132
Size: 1510 272 1018 3774
Fragments arranged by size:
3774 1510 1018 272
SspI AAT'ATT
Cuts at: 5 52 4498 5
Size: 47 4446 2081
Fragments arranged by size:
4446 2081 47
StuI AGG'CCT
Cuts at: 1517 2045 2627 2684 2963 3548 1517
Size: 528 582 57 279 585 4543
Fragments arranged by size:
4543 585 582 528 279 57
StyI C'CwwG_G
Cuts at: 513 1065 2004 2226 2502 3480 3549 3660 3867
Size: 552 939 222 276 978 69 111 207
Cuts at: 3867 4013 513
Size: 146 3074
Fragments arranged by size:
3074 978 939 552 276 222 207 146
111 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3306 3773 4601 4618 4771 4956 5401
Size: 1255 1726 467 828 17 153 185 445
Cuts at: 5401 325
Size: 1498
Fragments arranged by size:
1726 1498 1255 828 467 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4820 6532 96
Size: 274 80 33 51 1443 2843 1712 138
Fragments arranged by size:
2843 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2649 2660 2681 2692 6534 6566 2649
Size: 11 21 11 3842 32 2657
Fragments arranged by size:
3842 2657 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6574
XbaI T'CTAG_A
Cuts at: 2548 3910 2548
Size: 1362 5212
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2374 3619 3730 2374
Size: 1245 111 5218
Fragments arranged by size:
5218 1245 111
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6574
XmaI C'CCGG_G
Cuts at: 1870 3899 1870
Size: 2029 4545
XmnI GAAnn'nnTTC
Cuts at: 4703 4703
Size: 6574
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4382 276
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI SacI SacII SanDI SfiI
SfoI SrfI SwaI