Restriction map for
pFN21AE4540
(Circular) (Six-base) MAPSORT of: pFN21AE4540.seq Check: 4990 from: 1 to: 6571
pFN21AE4540 6571 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2676 2676
Size: 6571
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4381 278
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3313 3914 1937
Size: 1376 601 4594
Fragments arranged by size:
4594 1376 601
Acc65I G'GTAC_C
Cuts at: 3892 3892
Size: 6571
AclI AA'CG_TT
Cuts at: 1563 4698 5071 1563
Size: 3135 373 3063
Fragments arranged by size:
3135 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2231 3006 3123 4634 6018 6286 1632
Size: 599 775 117 1511 1384 268 1917
Fragments arranged by size:
1917 1511 1384 775 599 268 117
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6374
AflIII A'CryG_T
Cuts at: 1203 5470 6447 1203
Size: 4267 977 1327
Fragments arranged by size:
4267 1327 977
AgeI A'CCGG_T
Cuts at: 5402 5402
Size: 6571
AhdI GACnn_n'nnGTC
Cuts at: 5300 5300
Size: 6571
AleI CACnn'nnGTG
Cuts at: 1166 2139 1166
Size: 973 5598
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6571
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3608 3640 3608
Size: 32 6539
AlwNI CAG_nnn'CTG
Cuts at: 2865 3404 4314 5886 2865
Size: 539 910 1572 3550
Fragments arranged by size:
3550 1572 910 539
ApaLI G'TGCA_C
Cuts at: 4628 5784 4628
Size: 1156 5415
ApoI r'AATT_y
Cuts at: 1493 3575 3752 3880 4106 4258 6367 1493
Size: 2082 177 128 226 152 2109 1697
Fragments arranged by size:
2109 2082 1697 226 177 152 128
AseI AT'TA_AT
Cuts at: 160 1032 5125 160
Size: 872 4093 1606
Fragments arranged by size:
4093 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
AvaI C'yCGr_G
Cuts at: 1870 1943 2096 2726 2906 3214 3896 1870
Size: 73 153 630 180 308 682 4545
Fragments arranged by size:
4545 682 630 308 180 153 73
BamHI G'GATC_C
Cuts at: 3901 4268 3901
Size: 367 6204
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2595 3892 5347 618
Size: 325 217 627 808 1297 1455 1842
Fragments arranged by size:
1842 1455 1297 808 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2456 3182 3890 1391
Size: 1065 726 708 4072
Fragments arranged by size:
4072 1065 726 708
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2879 3035 3780 928
Size: 971 980 156 745 3719
Fragments arranged by size:
3719 980 971 745 156
BbvCI CC'TCA_GC
Cuts at: 2013 2333 2013
Size: 320 6251
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4762 4796 1903
Size: 34 50 34 2741 34 3678
Fragments arranged by size:
3678 2741 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4463 5673 4463
Size: 1210 5361
BclI T'GATC_A
Cuts at: 1548 2125 1548
Size: 577 5994
BfrBI ATG'CAT
Cuts at: 3433 3433
Size: 6571
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3401 5182 136
Size: 107 122 71 631 605 1729 1781 1525
Fragments arranged by size:
1781 1729 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3534 6566 3534
Size: 3032 3539
BlpI GC'TnA_GC
Cuts at: 2109 3497 3988 2109
Size: 1388 491 4692
Fragments arranged by size:
4692 1388 491
Bme1580I G_kGCm'C
Cuts at: 1790 2316 2537 2600 3205 3245 3717 4632 5788
Size: 526 221 63 605 40 472 915 1156
Cuts at: 5788 1790
Size: 2573
Fragments arranged by size:
2573 1156 915 605 526 472 221 63
40
BmgBI CAC'GTC
Cuts at: 1318 3156 1318
Size: 1838 4733
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2564 3121 4302
Size: 416 53 83 399 24 1147 557 1181
Cuts at: 4302 5260 442
Size: 958 1753
Fragments arranged by size:
1753 1181 1147 958 557 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3137 1056
Size: 2081 4490
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2707 3196 2707
Size: 489 6082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3589 3635 3812 5231 1239
Size: 2350 46 177 1419 2579
Fragments arranged by size:
2579 2350 1419 177 46
Bpu10I CC'TnA_GC
Cuts at: 2013 2333 2013
Size: 320 6251
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4696 5561 5859 4696
Size: 865 298 5408
Fragments arranged by size:
5408 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3083 5234 882
Size: 617 1584 2151 2219
Fragments arranged by size:
2219 2151 1584 617
BsaAI yAC'GTr
Cuts at: 493 1204 2500 4362 493
Size: 711 1296 1862 2702
Fragments arranged by size:
2702 1862 1296 711
BsaBI GATnn'nnATC
Cuts at: 2474 6565 2474
Size: 4091 2480
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4378 4760 5395 275
Size: 53 83 186 936 2845 382 635 1451
Fragments arranged by size:
2845 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3271 3555 3630 5004 5402 5676 5823 6234 3271
Size: 284 75 1374 398 274 147 411 3608
Fragments arranged by size:
3608 1374 411 398 284 274 147 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2765 2795 3354 3384 3608
Size: 30 165 30 1362 30 559 30 224
Cuts at: 3608 3638 1178
Size: 30 4111
Fragments arranged by size:
4111 1362 559 224 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2001 2168 2171 2375 1186
Size: 815 167 3 204 5382
Fragments arranged by size:
5382 815 204 167 3
BseYI C'CCAG_C
Cuts at: 2449 2858 2897 3507 3514 5774 6270 2449
Size: 409 39 610 7 2260 496 2750
Fragments arranged by size:
2750 2260 610 496 409 39 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3208 1163
Size: 573 1472 4526
Fragments arranged by size:
4526 1472 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2347 2850 4021 4782 5810 1540
Size: 275 186 346 503 1171 761 1028 2301
Fragments arranged by size:
2301 1171 1028 761 503 346 275 186
BsiHKAI G_wGCw'C
Cuts at: 2796 3182 4632 4717 5788 2796
Size: 386 1450 85 1071 3579
Fragments arranged by size:
3579 1450 1071 386 85
BsmI GAATG_Cn'
Cuts at: 4082 4175 4082
Size: 93 6478
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2316 2456 2537 2600 2796 3182 3205
Size: 399 526 140 81 63 196 386 23
Cuts at: 3205 3245 3717 3890 4632 4717 5788 1391
Size: 40 472 173 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 526 472 399 386 196
173 140 85 81 63 40 23
BspEI T'CCGG_A
Cuts at: 3271 3555 6234 3271
Size: 284 2679 3608
Fragments arranged by size:
3608 2679 284
BspHI T'CATG_A
Cuts at: 2520 4460 6190 2520
Size: 1940 1730 2901
Fragments arranged by size:
2901 1940 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2676 3325 3926 844
Size: 1832 649 601 3489
Fragments arranged by size:
3489 1832 649 601
BsrBI CCG'CTC
Cuts at: 1394 1823 4458 1394
Size: 429 2635 3507
Fragments arranged by size:
3507 2635 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2937 5066 5240 65
Size: 1183 1689 2129 174 1396
Fragments arranged by size:
2129 1689 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2760 5215 5402 1700
Size: 110 950 2455 187 2869
Fragments arranged by size:
2869 2455 950 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6571
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6571
BssSI C'ACGA_G
Cuts at: 4631 5643 4631
Size: 1012 5559
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6571
BstBI TT'CG_AA
Cuts at: 5375 5375
Size: 6571
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6571
BstXI CCAn_nnnn'nTGG
Cuts at: 2257 2764 3613 2257
Size: 507 849 5215
Fragments arranged by size:
5215 849 507
BstYI r'GATC_y
Cuts at: 1972 3534 3837 3901 4268 4653 4670 6122 6208
Size: 1562 303 64 367 385 17 1452 86
Cuts at: 6208 6220 6566 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1562 1452 385 367 346 303 86
64 17 12
Bsu36I CC'TnA_GG
Cuts at: 2201 2823 2201
Size: 622 5949
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6019
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2574 2644 487
Size: 683 56 1348 70 4414
Fragments arranged by size:
4414 1348 683 70 56
BtsI GCAGTG_nn'
Cuts at: 2088 2707 4087 4881 4901 2088
Size: 619 1380 794 20 3758
Fragments arranged by size:
3758 1380 794 619 20
ClaI AT'CG_AT
Cuts at: 1554 3636 1554
Size: 2082 4489
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6536
DraI TTT'AAA
Cuts at: 3875 3875
Size: 6571
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5578 809
Size: 507 4262 1802
Fragments arranged by size:
4262 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2259 2505
Size: 54 1425 50 275 18 210 219 246
Cuts at: 2505 2754 2766 2847 2883 3084 3378 3584 4018
Size: 249 12 81 36 201 294 206 434
Cuts at: 4018 4907 6536 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 434 294 275 249 246
219 210 206 201 81 54 50 43
36 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2847 4018 1537
Size: 275 1035 1171 4090
Fragments arranged by size:
4090 1171 1035 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2184 4501 1353
Size: 831 2317 3423
Fragments arranged by size:
3423 2317 831
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5158 5532 5678 387
Size: 1064 138 3569 374 146 1280
Fragments arranged by size:
3569 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3180 3180
Size: 6571
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 3006 3123 3589 3635 3812 4634
Size: 393 599 775 117 466 46 177 822
Cuts at: 4634 5231 6018 6286 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 822 787 775 599 597 466 393
268 177 117 46
EcoNI CCTnn'n_nnAGG
Cuts at: 2075 2075
Size: 6571
EcoRI G'AATT_C
Cuts at: 3752 3880 4258 3752
Size: 128 378 6065
Fragments arranged by size:
6065 378 128
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 6571
FspI TGC'GCA
Cuts at: 5077 5077
Size: 6571
HaeII r_GCGC'y
Cuts at: 1678 2865 5718 1678
Size: 1187 2853 2531
Fragments arranged by size:
2853 2531 1187
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2673 2705 5212 5244 5286
Size: 32 135 32 1101 32 2507 32 42
Cuts at: 5286 5318 1373
Size: 32 2626
Fragments arranged by size:
2626 2507 1101 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2626 3547 3915 4161 669
Size: 988 281 688 921 368 246 3079
Fragments arranged by size:
3079 988 921 688 368 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6571
HpaI GTT'AAC
Cuts at: 4161 4161
Size: 6571
KpnI G_GTAC'C
Cuts at: 3896 3896
Size: 6571
MfeI C'AATT_G
Cuts at: 4170 4170
Size: 6571
MluI A'CGCG_T
Cuts at: 6447 6447
Size: 6571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5685 5869 1305
Size: 4380 184 2007
Fragments arranged by size:
4380 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 2507 2756 2768 2885
Size: 54 1425 343 429 246 249 12 117
Cuts at: 2885 3586 10
Size: 701 2995
Fragments arranged by size:
2995 1425 701 429 343 249 246 117
54 12
MslI CAynn'nnrTG
Cuts at: 518 1166 2139 3722 4529 4888 5047 518
Size: 648 973 1583 807 359 159 2042
Fragments arranged by size:
2042 1583 973 807 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2449 3351 3401 3987 4664 5812 6057
Size: 411 432 902 50 586 677 1148 245
Cuts at: 6057 1606
Size: 2120
Fragments arranged by size:
2120 1148 902 677 586 432 411 245
50
NaeI GCC'GGC
Cuts at: 2762 2762
Size: 6571
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6019
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6571
NgoMIV G'CCGG_C
Cuts at: 2760 2760
Size: 6571
NheI G'CTAG_C
Cuts at: 1052 3133 1052
Size: 2081 4490
NotI GC'GGCC_GC
Cuts at: 2847 4018 2847
Size: 1171 5400
NsiI A_TGCA'T
Cuts at: 3435 3435
Size: 6571
NspI r_CATG'y
Cuts at: 1132 1229 2515 2800 3929 5474 1132
Size: 97 1286 285 1129 1545 2229
Fragments arranged by size:
2229 1545 1286 1129 285 97
PasI CC'CwG_GG
Cuts at: 1348 2250 2396 1348
Size: 902 146 5523
Fragments arranged by size:
5523 902 146
PciI A'CATG_T
Cuts at: 5470 5470
Size: 6571
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2590 2993 3593 1108
Size: 1482 403 600 4086
Fragments arranged by size:
4086 1482 600 403
PfoI T'CCnGG_A
Cuts at: 2544 3158 2544
Size: 614 5957
PmeI GTTT'AAAC
Cuts at: 3875 3875
Size: 6571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2363 2395 3608 3640 4640 4672 6153 6185 2363
Size: 32 1213 32 1000 32 1481 32 2749
Fragments arranged by size:
2749 1481 1213 1000 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6571
PsiI TTA'TAA
Cuts at: 4141 4141
Size: 6571
PspXI vC'TCGA_Gb
Cuts at: 3214 3214
Size: 6571
PstI C_TGCA'G
Cuts at: 830 3409 3923 830
Size: 2579 514 3478
Fragments arranged by size:
3478 2579 514
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6571
PvuII CAG'CTG
Cuts at: 2017 3351 2017
Size: 1334 5237
SacI G_AGCT'C
Cuts at: 3182 3182
Size: 6571
SalI G'TCGA_C
Cuts at: 1936 3913 1936
Size: 1977 4594
SbfI CC_TGCA'GG
Cuts at: 3923 3923
Size: 6571
ScaI AGT'ACT
Cuts at: 4819 6531 4819
Size: 1712 4859
SfcI C'TryA_G
Cuts at: 826 1045 3405 3919 5054 5735 5926 826
Size: 219 2360 514 1135 681 191 1471
Fragments arranged by size:
2360 1471 1135 681 514 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6571
SmaI CCC'GGG
Cuts at: 1872 2908 3898 1872
Size: 1036 990 4545
Fragments arranged by size:
4545 1036 990
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2096 3214 4675 5576 5838 820
Size: 197 926 153 1118 1461 901 262 1553
Fragments arranged by size:
1553 1461 1118 926 901 262 197 153
SnaBI TAC'GTA
Cuts at: 493 4362 493
Size: 3869 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6571
SphI G_CATG'C
Cuts at: 1132 2515 3929 1132
Size: 1383 1414 3774
Fragments arranged by size:
3774 1414 1383
SspI AAT'ATT
Cuts at: 5 52 4495 5
Size: 47 4443 2081
Fragments arranged by size:
4443 2081 47
StuI AGG'CCT
Cuts at: 1517 2331 3708 1517
Size: 814 1377 4380
Fragments arranged by size:
4380 1377 814
StyI C'CwwG_G
Cuts at: 513 1065 2769 3681 3747 4010 513
Size: 552 1704 912 66 263 3074
Fragments arranged by size:
3074 1704 912 552 263 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2306 2333 3528 4598 4615 4768 4953
Size: 1255 726 27 1195 1070 17 153 185
Cuts at: 4953 5398 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1195 1070 726 445 185 153
27 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4817 6529 96
Size: 274 80 33 51 1443 2840 1712 138
Fragments arranged by size:
2840 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2765 2797 3354 3386 6531 6563 2765
Size: 32 557 32 3145 32 2773
Fragments arranged by size:
3145 2773 557 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6571
XbaI T'CTAG_A
Cuts at: 3907 3907
Size: 6571
XhoI C'TCGA_G
Cuts at: 1943 2096 3214 1943
Size: 153 1118 5300
Fragments arranged by size:
5300 1118 153
XmaI C'CCGG_G
Cuts at: 1870 2906 3896 1870
Size: 1036 990 4545
Fragments arranged by size:
4545 1036 990
XmnI GAAnn'nnTTC
Cuts at: 2378 3279 4700 2378
Size: 901 1421 4249
Fragments arranged by size:
4249 1421 901
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4379 276
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI
BstZ17I DraIII EcoO109I EcoRV FseI FspAI KasI NarI
NruI PacI PmlI PpuMI PspOMI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI