Restriction map for
pFN21AE4537
(Circular) (Six-base) MAPSORT of: pFN21AE4537.seq Check: 583 from: 1 to: 6520
pFN21AE4537 6520 bp
With 182 enzymes: SgfI *
March 28, 2012 14:11 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4330 278
Size: 53 83 186 936 2794 2468
Fragments arranged by size:
2794 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3863 1937
Size: 1926 4594
Acc65I G'GTAC_C
Cuts at: 3841 3841
Size: 6520
AclI AA'CG_TT
Cuts at: 1563 4647 5020 1563
Size: 3084 373 3063
Fragments arranged by size:
3084 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2130 2839 4583 5967 6235 1632
Size: 498 709 1744 1384 268 1917
Fragments arranged by size:
1917 1744 1384 709 498 268
AflII C'TTAA_G
Cuts at: 820 1017 3667 820
Size: 197 2650 3673
Fragments arranged by size:
3673 2650 197
AflIII A'CryG_T
Cuts at: 1203 5419 6396 1203
Size: 4216 977 1327
Fragments arranged by size:
4216 1327 977
AgeI A'CCGG_T
Cuts at: 5351 5351
Size: 6520
AhdI GACnn_n'nnGTC
Cuts at: 5249 5249
Size: 6520
AleI CACnn'nnGTG
Cuts at: 1166 2574 1166
Size: 1408 5112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6520
AlwNI CAG_nnn'CTG
Cuts at: 4263 5835 4263
Size: 1572 4948
ApaLI G'TGCA_C
Cuts at: 4577 5733 4577
Size: 1156 5364
ApoI r'AATT_y
Cuts at: 1493 2598 2864 3295 3346 3538 3829 4055 4207
Size: 1105 266 431 51 192 291 226 152
Cuts at: 4207 6316 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1105 431 291 266 226 192
152 51
AseI AT'TA_AT
Cuts at: 160 1032 5074 160
Size: 872 4042 1606
Fragments arranged by size:
4042 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
AvaI C'yCGr_G
Cuts at: 1870 1943 2194 2220 2352 3375 3845 1870
Size: 73 251 26 132 1023 470 4545
Fragments arranged by size:
4545 1023 470 251 132 73 26
BamHI G'GATC_C
Cuts at: 3277 3850 4217 3277
Size: 573 367 5580
Fragments arranged by size:
5580 573 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2263 3841 5296 618
Size: 325 217 627 476 1578 1455 1842
Fragments arranged by size:
1842 1578 1455 627 476 325 217
BanII G_rGCy'C
Cuts at: 1391 2097 3839 1391
Size: 706 1742 4072
Fragments arranged by size:
4072 1742 706
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2029 2495 928
Size: 971 130 466 4953
Fragments arranged by size:
4953 971 466 130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4711 4745 1903
Size: 34 2774 34 3678
Fragments arranged by size:
3678 2774 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3453 4412 5622 3453
Size: 959 1210 4351
Fragments arranged by size:
4351 1210 959
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6520
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2158 2225 2261
Size: 107 122 71 631 605 486 67 36
Cuts at: 2261 5131 136
Size: 2870 1525
Fragments arranged by size:
2870 1525 631 605 486 122 107 71
67 36
BglII A'GATC_T
Cuts at: 6515 6515
Size: 6520
BlpI GC'TnA_GC
Cuts at: 3687 3937 3687
Size: 250 6270
Bme1580I G_kGCm'C
Cuts at: 1790 2325 4581 5737 1790
Size: 535 2256 1156 2573
Fragments arranged by size:
2573 2256 1156 535
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6520
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4251 5209 442
Size: 416 53 83 399 24 2834 958 1753
Fragments arranged by size:
2834 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3097 3117 3290 5180 1239
Size: 1858 20 173 1890 2579
Fragments arranged by size:
2579 1890 1858 173 20
Bpu10I CC'TnA_GC
Cuts at: 2412 2412
Size: 6520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4645 5510 5808 4645
Size: 865 298 5357
Fragments arranged by size:
5357 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5183 882
Size: 617 3684 2219
Fragments arranged by size:
3684 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2572 4311 493
Size: 711 1368 1739 2702
Fragments arranged by size:
2702 1739 1368 711
BsaBI GATnn'nnATC
Cuts at: 6514 6514
Size: 6520
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2247 4327 4709 5344
Size: 53 83 186 936 714 2080 382 635
Cuts at: 5344 275
Size: 1451
Fragments arranged by size:
2080 1451 936 714 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4953 5351 5625 5772 6183 4953
Size: 398 274 147 411 5290
Fragments arranged by size:
5290 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2019 2049 2348 2378 3070
Size: 30 165 30 616 30 299 30 692
Cuts at: 3070 3100 1178
Size: 30 4598
Fragments arranged by size:
4598 692 616 299 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3017 3287 1186
Size: 1831 270 4419
Fragments arranged by size:
4419 1831 270
BseYI C'CCAG_C
Cuts at: 2023 2206 5723 6219 2023
Size: 183 3517 496 2324
Fragments arranged by size:
3517 2324 496 183
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5947
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2135 2162 2172 3970 4731 5759
Size: 275 186 134 27 10 1798 761 1028
Cuts at: 5759 1540
Size: 2301
Fragments arranged by size:
2301 1798 1028 761 275 186 134 27
10
BsiHKAI G_wGCw'C
Cuts at: 2361 2786 2827 3688 4581 4666 5737 2361
Size: 425 41 861 893 85 1071 3144
Fragments arranged by size:
3144 1071 893 861 425 85 41
BsmI GAATG_Cn'
Cuts at: 4031 4124 4031
Size: 93 6427
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2325 2361 2786 2827 3688 3839
Size: 399 307 228 36 425 41 861 151
Cuts at: 3839 4581 4666 5737 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 861 742 425 399 307 228
151 85 41 36
BspEI T'CCGG_A
Cuts at: 6183 6183
Size: 6520
BspHI T'CATG_A
Cuts at: 4409 6139 4409
Size: 1730 4790
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3322 3875 844
Size: 2478 553 3489
Fragments arranged by size:
3489 2478 553
BsrBI CCG'CTC
Cuts at: 1394 1823 4407 1394
Size: 429 2584 3507
Fragments arranged by size:
3507 2584 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3337 5015 5189 65
Size: 1183 2089 1678 174 1396
Fragments arranged by size:
2089 1678 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2167 2260 2407 5164 5351 1700
Size: 110 357 93 147 2757 187 2869
Fragments arranged by size:
2869 2757 357 187 147 110 93
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6520
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6520
BssSI C'ACGA_G
Cuts at: 4580 5592 4580
Size: 1012 5508
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2348 3088 794
Size: 1554 740 4226
Fragments arranged by size:
4226 1554 740
BstBI TT'CG_AA
Cuts at: 5324 5324
Size: 6520
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6520
BstYI r'GATC_y
Cuts at: 1972 3277 3412 3850 4217 4602 4619 6071 6157
Size: 1305 135 438 367 385 17 1452 86
Cuts at: 6157 6169 6515 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1305 438 385 367 346 135
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2126 2156 2295 3281 513
Size: 552 939 122 30 139 986 3752
Fragments arranged by size:
3752 986 939 552 139 122 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5781
Fragments arranged by size:
5781 683 56
BtsI GCAGTG_nn'
Cuts at: 4036 4830 4850 4036
Size: 794 20 5706
Fragments arranged by size:
5706 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6520
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6485
DraI TTT'AAA
Cuts at: 3607 3824 3607
Size: 217 6303
DraIII CAC_nnn'GTG
Cuts at: 2831 2831
Size: 6520
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2894 5527 809
Size: 507 1578 2633 1802
Fragments arranged by size:
2633 1802 1578 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2159 2169 2483
Size: 54 1425 50 275 18 329 10 314
Cuts at: 2483 3284 3559 3967 4856 6485 8
Size: 801 275 408 889 1629 43
Fragments arranged by size:
1629 1425 889 801 408 329 314 275
275 54 50 43 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 2159 2169 3967 1537
Size: 275 347 10 1798 4090
Fragments arranged by size:
4090 1798 347 275 10
EarI CTCTTCn'nnn_
Cuts at: 1353 3615 4450 1353
Size: 2262 835 3423
Fragments arranged by size:
3423 2262 835
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2492 5107 5481 5627 387
Size: 1064 138 903 2615 374 146 1280
Fragments arranged by size:
2615 1280 1064 903 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2130 2839 3097 3117 3290 4583 5180
Size: 393 498 709 258 20 173 1293 597
Cuts at: 5180 5967 6235 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1293 787 709 597 498 393 268
258 173 20
EcoO109I rG'GnC_Cy
Cuts at: 2253 2253
Size: 6520
EcoRI G'AATT_C
Cuts at: 2598 3295 3829 4207 2598
Size: 697 534 378 4911
Fragments arranged by size:
4911 697 534 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2638 2638
Size: 6520
FspI TGC'GCA
Cuts at: 5026 5026
Size: 6520
HaeII r_GCGC'y
Cuts at: 1678 5667 1678
Size: 3989 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2019 2051 2670 2702 2739
Size: 32 135 32 447 32 619 32 37
Cuts at: 2739 2771 2966 2998 3369 3401 3538 3570 5161
Size: 32 195 32 371 32 137 32 1591
Cuts at: 5161 5193 5235 5267 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1591 619 447 371 195 137 135
42 37 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3552 3864 4110 669
Size: 988 281 1614 312 246 3079
Fragments arranged by size:
3079 1614 988 312 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6520
HpaI GTT'AAC
Cuts at: 4110 4110
Size: 6520
KpnI G_GTAC'C
Cuts at: 3845 3845
Size: 6520
MfeI C'AATT_G
Cuts at: 3489 4119 3489
Size: 630 5890
MluI A'CGCG_T
Cuts at: 6396 6396
Size: 6520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2067 3620 5634 5818 1305
Size: 762 1553 2014 184 2007
Fragments arranged by size:
2014 2007 1553 762 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2485 3286 3561 10
Size: 54 1425 343 653 801 275 2969
Fragments arranged by size:
2969 1425 801 653 343 275 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2555 2574 2984 3524 4478 4837 4996
Size: 648 1389 19 410 540 954 359 159
Cuts at: 4996 518
Size: 2042
Fragments arranged by size:
2042 1389 954 648 540 410 359 159
19
MspA1I CmG'CkG
Cuts at: 1606 2128 2158 2210 2274 3075 3365 3936 4613
Size: 522 30 52 64 801 290 571 677
Cuts at: 4613 5761 6006 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 801 677 571 522 290 245
64 52 30
NaeI GCC'GGC
Cuts at: 2169 2262 2409 2169
Size: 93 147 6280
Fragments arranged by size:
6280 147 93
NcoI C'CATG_G
Cuts at: 513 1065 2004 3281 513
Size: 552 939 1277 3752
Fragments arranged by size:
3752 1277 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6520
NgoMIV G'CCGG_C
Cuts at: 2167 2260 2407 2167
Size: 93 147 6280
Fragments arranged by size:
6280 147 93
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6520
NotI GC'GGCC_GC
Cuts at: 3967 3967
Size: 6520
NruI TCG'CGA
Cuts at: 2232 2232
Size: 6520
NspI r_CATG'y
Cuts at: 1132 1229 3878 5423 1132
Size: 97 2649 1545 2229
Fragments arranged by size:
2649 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6520
PciI A'CATG_T
Cuts at: 5419 5419
Size: 6520
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6520
PfoI T'CCnGG_A
Cuts at: 2241 2241
Size: 6520
PmeI GTTT'AAAC
Cuts at: 3824 3824
Size: 6520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4589 4621 6102 6134 4589
Size: 32 1481 32 4975
Fragments arranged by size:
4975 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6520
PsiI TTA'TAA
Cuts at: 4090 4090
Size: 6520
PstI C_TGCA'G
Cuts at: 830 3872 830
Size: 3042 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6520
PvuII CAG'CTG
Cuts at: 3365 3365
Size: 6520
SacII CC_GC'GG
Cuts at: 2129 2159 2129
Size: 30 6490
SalI G'TCGA_C
Cuts at: 1936 3862 1936
Size: 1926 4594
SbfI CC_TGCA'GG
Cuts at: 3872 3872
Size: 6520
ScaI AGT'ACT
Cuts at: 4768 6480 4768
Size: 1712 4808
SfcI C'TryA_G
Cuts at: 826 1045 3868 5003 5684 5875 826
Size: 219 2823 1135 681 191 1471
Fragments arranged by size:
2823 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6520
SmaI CCC'GGG
Cuts at: 1872 2354 3847 1872
Size: 482 1493 4545
Fragments arranged by size:
4545 1493 482
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3375 3667 4624 5525 5787 820
Size: 197 926 1432 292 957 901 262 1553
Fragments arranged by size:
1553 1432 957 926 901 292 262 197
SnaBI TAC'GTA
Cuts at: 493 4311 493
Size: 3818 2702
SpeI A'CTAG_T
Cuts at: 152 3107 152
Size: 2955 3565
SphI G_CATG'C
Cuts at: 1132 3878 1132
Size: 2746 3774
SspI AAT'ATT
Cuts at: 5 52 4444 5
Size: 47 4392 2081
Fragments arranged by size:
4392 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6520
StyI C'CwwG_G
Cuts at: 513 1065 2004 2670 3281 3287 3388 3959 513
Size: 552 939 666 611 6 101 571 3074
Fragments arranged by size:
3074 939 666 611 571 552 101 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3011 4547 4564 4717 4902 5347 325
Size: 1255 1431 1536 17 153 185 445 1498
Fragments arranged by size:
1536 1498 1431 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2016 2061 4766
Size: 274 80 33 51 1443 39 45 2705
Cuts at: 4766 6478 96
Size: 1712 138
Fragments arranged by size:
2705 1712 1443 274 138 80 51 45
39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2348 2380 6480 6512 2348
Size: 32 4100 32 2356
Fragments arranged by size:
4100 2356 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2624 1316
Size: 1308 5212
XbaI T'CTAG_A
Cuts at: 3856 3856
Size: 6520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3770 3770
Size: 6520
XhoI C'TCGA_G
Cuts at: 1943 3375 1943
Size: 1432 5088
XmaI C'CCGG_G
Cuts at: 1870 2352 3845 1870
Size: 482 1493 4545
Fragments arranged by size:
4545 1493 482
XmnI GAAnn'nnTTC
Cuts at: 2390 4649 2390
Size: 2259 4261
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4328 276
Size: 53 83 186 936 2794 2468
Fragments arranged by size:
2794 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI