Restriction map for
pFN21AE4534
(Circular) (Six-base) MAPSORT of: pFN21AE4534.seq Check: 8025 from: 1 to: 6502
pFN21AE4534 6502 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4312 278
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2724 2952 3845 1937
Size: 787 228 893 4594
Fragments arranged by size:
4594 893 787 228
Acc65I G'GTAC_C
Cuts at: 3823 3823
Size: 6502
AclI AA'CG_TT
Cuts at: 1563 4629 5002 1563
Size: 3066 373 3063
Fragments arranged by size:
3066 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3713 4565 5949 6217 1632
Size: 2081 852 1384 268 1917
Fragments arranged by size:
2081 1917 1384 852 268
AfeI AGC'GCT
Cuts at: 2566 2566
Size: 6502
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6305
AflIII A'CryG_T
Cuts at: 1203 2139 3666 5401 6378 1203
Size: 936 1527 1735 977 1327
Fragments arranged by size:
1735 1527 1327 977 936
AgeI A'CCGG_T
Cuts at: 5333 5333
Size: 6502
AhdI GACnn_n'nnGTC
Cuts at: 5231 5231
Size: 6502
AleI CACnn'nnGTG
Cuts at: 1166 2482 1166
Size: 1316 5186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3398 1184
Size: 2214 4288
AlwNI CAG_nnn'CTG
Cuts at: 3480 4245 5817 3480
Size: 765 1572 4165
Fragments arranged by size:
4165 1572 765
ApaLI G'TGCA_C
Cuts at: 2989 4559 5715 2989
Size: 1570 1156 3776
Fragments arranged by size:
3776 1570 1156
ApoI r'AATT_y
Cuts at: 1493 3811 4037 4189 6298 1493
Size: 2318 226 152 2109 1697
Fragments arranged by size:
2318 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5056 160
Size: 872 4024 1606
Fragments arranged by size:
4024 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
AvaI C'yCGr_G
Cuts at: 1870 1943 2789 2921 3086 3650 3827 1870
Size: 73 846 132 165 564 177 4545
Fragments arranged by size:
4545 846 564 177 165 132 73
BamHI G'GATC_C
Cuts at: 2670 3470 3832 4199 2670
Size: 800 362 367 4973
Fragments arranged by size:
4973 800 367 362
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3823 5278 618
Size: 325 217 627 2036 1455 1842
Fragments arranged by size:
2036 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3270 3689 3821 1391
Size: 1879 419 132 4072
Fragments arranged by size:
4072 1879 419 132
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3334 3624 3735 928
Size: 971 1435 290 111 3695
Fragments arranged by size:
3695 1435 971 290 111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4693 4727 1903
Size: 34 2756 34 3678
Fragments arranged by size:
3678 2756 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4394 5604 4394
Size: 1210 5292
BclI T'GATC_A
Cuts at: 1548 2757 1548
Size: 1209 5293
BfrBI ATG'CAT
Cuts at: 2596 2596
Size: 6502
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2069 5113 136
Size: 107 122 71 631 605 397 3044 1525
Fragments arranged by size:
3044 1525 631 605 397 122 107 71
BglII A'GATC_T
Cuts at: 2040 2877 6497 2040
Size: 837 3620 2045
Fragments arranged by size:
3620 2045 837
BlpI GC'TnA_GC
Cuts at: 3919 3919
Size: 6502
Bme1580I G_kGCm'C
Cuts at: 1790 2993 4563 5719 1790
Size: 1203 1570 1156 2573
Fragments arranged by size:
2573 1570 1203 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6502
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4233 5191 442
Size: 416 53 83 399 24 2816 958 1753
Fragments arranged by size:
2816 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2227 2324 5162 1239
Size: 988 97 2838 2579
Fragments arranged by size:
2838 2579 988 97
Bpu10I CC'TnA_GC
Cuts at: 3756 3756
Size: 6502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4627 5492 5790 4627
Size: 865 298 5339
Fragments arranged by size:
5339 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3314 5165 882
Size: 617 1815 1851 2219
Fragments arranged by size:
2219 1851 1815 617
BsaAI yAC'GTr
Cuts at: 493 1204 2142 4293 493
Size: 711 938 2151 2702
Fragments arranged by size:
2702 2151 938 711
BsaBI GATnn'nnATC
Cuts at: 6496 6496
Size: 6502
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4309 4691 5326 275
Size: 53 83 186 936 2776 382 635 1451
Fragments arranged by size:
2776 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4935 5333 5607 5754 6165 4935
Size: 398 274 147 411 5272
Fragments arranged by size:
5272 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2945 2975 1178
Size: 30 165 30 1542 30 4705
Fragments arranged by size:
4705 1542 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3239 1186
Size: 2053 4449
BseYI C'CCAG_C
Cuts at: 2896 3123 3178 3722 5705 6201 2896
Size: 227 55 544 1983 496 3197
Fragments arranged by size:
3197 1983 544 496 227 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2126 3184 3575 1163
Size: 573 390 1058 391 4090
Fragments arranged by size:
4090 1058 573 391 390
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2134 3952 4713 5741 1540
Size: 275 186 133 1818 761 1028 2301
Fragments arranged by size:
2301 1818 1028 761 275 186 133
BsiHKAI G_wGCw'C
Cuts at: 2312 2993 3270 4563 4648 5719 2312
Size: 681 277 1293 85 1071 3095
Fragments arranged by size:
3095 1293 1071 681 277 85
BsmI GAATG_Cn'
Cuts at: 4013 4106 4013
Size: 93 6409
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2312 2993 3270 3689 3821 4563 4648
Size: 399 522 681 277 419 132 742 85
Cuts at: 4648 5719 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 681 522 419 399 277
132 85
BspEI T'CCGG_A
Cuts at: 6165 6165
Size: 6502
BspHI T'CATG_A
Cuts at: 4391 6121 4391
Size: 1730 4772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3857 844
Size: 3013 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4389 1394
Size: 429 2566 3507
Fragments arranged by size:
3507 2566 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3060 3273 4997 5171 65
Size: 1183 1812 213 1724 174 1396
Fragments arranged by size:
1812 1724 1396 1183 213 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5146 5333 1700
Size: 110 3336 187 2869
Fragments arranged by size:
3336 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2457 96
Size: 2361 4141
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6502
BssSI C'ACGA_G
Cuts at: 3392 4562 5574 3392
Size: 1170 1012 4320
Fragments arranged by size:
4320 1170 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6502
BstBI TT'CG_AA
Cuts at: 5306 5306
Size: 6502
BstEII G'GTnAC_C
Cuts at: 1184 3118 1184
Size: 1934 4568
BstXI CCAn_nnnn'nTGG
Cuts at: 2092 2194 2569 2656 2092
Size: 102 375 87 5938
Fragments arranged by size:
5938 375 102 87
BstYI r'GATC_y
Cuts at: 1972 2040 2631 2670 2877 3470 3832 4199 4584
Size: 68 591 39 207 593 362 367 385
Cuts at: 4584 4601 6053 6139 6151 6497 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 593 591 385 367 362 346
207 86 68 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 3079 3079
Size: 6502
BtgI C'CryG_G
Cuts at: 513 1065 2004 2483 2773 3573 3636 513
Size: 552 939 479 290 800 63 3379
Fragments arranged by size:
3379 939 800 552 479 290 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2647 487
Size: 683 56 1421 4342
Fragments arranged by size:
4342 1421 683 56
BtsI GCAGTG_nn'
Cuts at: 2477 4018 4812 4832 2477
Size: 1541 794 20 4147
Fragments arranged by size:
4147 1541 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6502
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3507 3542 529
Size: 35 2943 35 3489
Fragments arranged by size:
3489 2943 35 35
DraI TTT'AAA
Cuts at: 3806 3806
Size: 6502
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5509 809
Size: 507 4193 1802
Fragments arranged by size:
4193 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2070 2639
Size: 54 1425 50 275 18 198 42 569
Cuts at: 2639 2646 2658 3949 4838 6467 8
Size: 7 12 1291 889 1629 43
Fragments arranged by size:
1629 1425 1291 889 569 275 198 54
50 43 42 18 12 7
EagI C'GGCC_G
Cuts at: 1537 1812 3949 1537
Size: 275 2137 4090
Fragments arranged by size:
4090 2137 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2300 2816 3047 3161 3275 4432 1353
Size: 947 516 231 114 114 1157 3423
Fragments arranged by size:
3423 1157 947 516 231 114 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5089 5463 5609 387
Size: 1064 138 3500 374 146 1280
Fragments arranged by size:
3500 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3268 3268
Size: 6502
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2227 2324 3713 4565 5162 5949 6217
Size: 393 595 97 1389 852 597 787 268
Cuts at: 6217 1239
Size: 1524
Fragments arranged by size:
1524 1389 852 787 597 595 393 268
97
EcoO109I rG'GnC_Cy
Cuts at: 2062 2287 2907 3083 3477 2062
Size: 225 620 176 394 5087
Fragments arranged by size:
5087 620 394 225 176
EcoRI G'AATT_C
Cuts at: 3811 4189 3811
Size: 378 6124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2170 2170
Size: 6502
FspI TGC'GCA
Cuts at: 5008 5008
Size: 6502
HaeII r_GCGC'y
Cuts at: 1678 2568 5649 1678
Size: 890 3081 2531
Fragments arranged by size:
3081 2531 890
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3000 3032 3347 3379 5143
Size: 32 135 32 1428 32 315 32 1764
Cuts at: 5143 5175 5217 5249 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1764 1428 315 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2545 3436 3846 4092 669
Size: 988 281 607 891 410 246 3079
Fragments arranged by size:
3079 988 891 607 410 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6502
HpaI GTT'AAC
Cuts at: 4092 4092
Size: 6502
KpnI G_GTAC'C
Cuts at: 3827 3827
Size: 6502
MfeI C'AATT_G
Cuts at: 4101 4101
Size: 6502
MluI A'CGCG_T
Cuts at: 6378 6378
Size: 6502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2553 3448 5616 5800 1305
Size: 1248 895 2168 184 2007
Fragments arranged by size:
2168 2007 1248 895 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2072 2641 2648 2660 10
Size: 54 1425 343 240 569 7 12 3852
Fragments arranged by size:
3852 1425 569 343 240 54 12 7
MslI CAynn'nnrTG
Cuts at: 518 1166 2482 2946 2996 4460 4819 4978 518
Size: 648 1316 464 50 1464 359 159 2042
Fragments arranged by size:
2042 1464 1316 648 464 359 159 50
MspA1I CmG'CkG
Cuts at: 1606 2520 2637 3127 3918 4595 5743 5988 1606
Size: 914 117 490 791 677 1148 245 2120
Fragments arranged by size:
2120 1148 914 791 677 490 245 117
NcoI C'CATG_G
Cuts at: 513 1065 2004 3573 3636 513
Size: 552 939 1569 63 3379
Fragments arranged by size:
3379 1569 939 552 63
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6502
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6502
NotI GC'GGCC_GC
Cuts at: 3949 3949
Size: 6502
NsiI A_TGCA'T
Cuts at: 2598 2598
Size: 6502
NspI r_CATG'y
Cuts at: 1132 1229 2596 3001 3670 3860 5405 1132
Size: 97 1367 405 669 190 1545 2229
Fragments arranged by size:
2229 1545 1367 669 405 190 97
PasI CC'CwG_GG
Cuts at: 1348 2314 1348
Size: 966 5536
PciI A'CATG_T
Cuts at: 3666 5401 3666
Size: 1735 4767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2667 3463 3610 3712 3749 1108
Size: 1559 796 147 102 37 3861
Fragments arranged by size:
3861 1559 796 147 102 37
PmeI GTTT'AAAC
Cuts at: 3806 3806
Size: 6502
PmlI CAC'GTG
Cuts at: 2142 2142
Size: 6502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4571 4603 6084 6116 4571
Size: 32 1481 32 4957
Fragments arranged by size:
4957 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2287 2907 2287
Size: 620 5882
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6502
PsiI TTA'TAA
Cuts at: 4072 4072
Size: 6502
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2405 2437 2405
Size: 32 6470
PstI C_TGCA'G
Cuts at: 830 3854 830
Size: 3024 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6502
PvuII CAG'CTG
Cuts at: 3127 3127
Size: 6502
SacI G_AGCT'C
Cuts at: 3270 3270
Size: 6502
SalI G'TCGA_C
Cuts at: 1936 3844 1936
Size: 1908 4594
SanDI GG'GwC_CC
Cuts at: 2287 2907 2287
Size: 620 5882
SapI GCTCTTCn'nnn_
Cuts at: 3275 3275
Size: 6502
SbfI CC_TGCA'GG
Cuts at: 3854 3854
Size: 6502
ScaI AGT'ACT
Cuts at: 4750 6462 4750
Size: 1712 4790
SfcI C'TryA_G
Cuts at: 826 1045 2533 2953 3850 4985 5666 5857 826
Size: 219 1488 420 897 1135 681 191 1471
Fragments arranged by size:
1488 1471 1135 897 681 420 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2069 2069
Size: 6502
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6502
SmaI CCC'GGG
Cuts at: 1872 2791 3088 3652 3829 1872
Size: 919 297 564 177 4545
Fragments arranged by size:
4545 919 564 297 177
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4606 5507 5769 820
Size: 197 926 2663 901 262 1553
Fragments arranged by size:
2663 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4293 493
Size: 3800 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6502
SphI G_CATG'C
Cuts at: 1132 3860 1132
Size: 2728 3774
SspI AAT'ATT
Cuts at: 5 52 4426 5
Size: 47 4374 2081
Fragments arranged by size:
4374 2081 47
StuI AGG'CCT
Cuts at: 1517 3794 1517
Size: 2277 4225
StyI C'CwwG_G
Cuts at: 513 1065 2004 2091 2796 3573 3636 3941 513
Size: 552 939 87 705 777 63 305 3074
Fragments arranged by size:
3074 939 777 705 552 305 87 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2839 3296 4529 4546 4699 4884 5329
Size: 1255 1259 457 1233 17 153 185 445
Cuts at: 5329 325
Size: 1498
Fragments arranged by size:
1498 1259 1255 1233 457 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 2457 4748
Size: 274 80 33 51 1443 345 135 2291
Cuts at: 4748 6460 96
Size: 1712 138
Fragments arranged by size:
2291 1712 1443 345 274 138 135 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2221 2253 2814 2846 6462 6494 2221
Size: 32 561 32 3616 32 2229
Fragments arranged by size:
3616 2229 561 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6502
XbaI T'CTAG_A
Cuts at: 3838 3838
Size: 6502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2092 2536 2569 3067 3464 2092
Size: 444 33 498 397 5130
Fragments arranged by size:
5130 498 444 397 33
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6502
XmaI C'CCGG_G
Cuts at: 1870 2789 3086 3650 3827 1870
Size: 919 297 564 177 4545
Fragments arranged by size:
4545 919 564 297 177
XmnI GAAnn'nnTTC
Cuts at: 4631 4631
Size: 6502
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4310 276
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PspOMI PspXI RsrII SacII SexAI SfoI SrfI SwaI