Restriction map for
pFN21AE4531
(Circular) (Six-base) MAPSORT of: pFN21AE4531.seq Check: 9321 from: 1 to: 6487
pFN21AE4531 6487 bp
With 182 enzymes: SgfI *
March 28, 2012 14:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2211 3642 2211
Size: 1431 5056
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4297 278
Size: 53 83 186 936 2761 2468
Fragments arranged by size:
2761 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3830 1937
Size: 1893 4594
Acc65I G'GTAC_C
Cuts at: 3501 3808 3501
Size: 307 6180
AclI AA'CG_TT
Cuts at: 1563 4614 4987 1563
Size: 3051 373 3063
Fragments arranged by size:
3063 3051 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2017 2509 4550 5934 6202 1632
Size: 385 492 2041 1384 268 1917
Fragments arranged by size:
2041 1917 1384 492 385 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6290
AflIII A'CryG_T
Cuts at: 1203 5386 6363 1203
Size: 4183 977 1327
Fragments arranged by size:
4183 1327 977
AgeI A'CCGG_T
Cuts at: 5318 5318
Size: 6487
AhdI GACnn_n'nnGTC
Cuts at: 5216 5216
Size: 6487
AleI CACnn'nnGTG
Cuts at: 1166 2468 1166
Size: 1302 5185
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6487
AlwNI CAG_nnn'CTG
Cuts at: 2449 2977 4230 5802 2449
Size: 528 1253 1572 3134
Fragments arranged by size:
3134 1572 1253 528
ApaI G_GGCC'C
Cuts at: 2187 2457 3405 2187
Size: 270 948 5269
Fragments arranged by size:
5269 948 270
ApaLI G'TGCA_C
Cuts at: 4544 5700 4544
Size: 1156 5331
ApoI r'AATT_y
Cuts at: 1493 3796 4022 4174 6283 1493
Size: 2303 226 152 2109 1697
Fragments arranged by size:
2303 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5041 160
Size: 872 4009 1606
Fragments arranged by size:
4009 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6487
AvaI C'yCGr_G
Cuts at: 1870 1943 2239 2268 2293 2418 3586 3812 1870
Size: 73 296 29 25 125 1168 226 4545
Fragments arranged by size:
4545 1168 296 226 125 73 29 25
BamHI G'GATC_C
Cuts at: 3817 4184 3817
Size: 367 6120
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2170 2347 2425 3501 3808
Size: 325 217 627 383 177 78 1076 307
Cuts at: 3808 5263 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1076 627 383 325 307 217
177 78
BanII G_rGCy'C
Cuts at: 1391 2187 2457 2913 3405 3460 3593 3806 1391
Size: 796 270 456 492 55 133 213 4072
Fragments arranged by size:
4072 796 492 456 270 213 133 55
BbeI G_GCGC'C
Cuts at: 2174 2351 2429 2174
Size: 177 78 6232
Fragments arranged by size:
6232 177 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3157 3183 928
Size: 971 1258 26 4232
Fragments arranged by size:
4232 1258 971 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4678 4712 1903
Size: 34 50 34 2657 34 3678
Fragments arranged by size:
3678 2657 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4379 5589 4379
Size: 1210 5277
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6487
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2250 2391 3311
Size: 107 122 71 631 605 578 141 920
Cuts at: 3311 3400 5098 136
Size: 89 1698 1525
Fragments arranged by size:
1698 1525 920 631 605 578 141 122
107 89 71
BglII A'GATC_T
Cuts at: 6482 6482
Size: 6487
BlpI GC'TnA_GC
Cuts at: 3904 3904
Size: 6487
Bme1580I G_kGCm'C
Cuts at: 1790 2187 2457 3405 4548 5704 1790
Size: 397 270 948 1143 1156 2573
Fragments arranged by size:
2573 1156 1143 948 397 270
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6487
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3109 4218 5176
Size: 416 53 83 399 24 1692 1109 958
Cuts at: 5176 442
Size: 1753
Fragments arranged by size:
1753 1692 1109 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6487
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2631 2697 2631
Size: 66 6421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2218 2642 3042 3497 3563 3746 5147 1239
Size: 979 424 400 455 66 183 1401 2579
Fragments arranged by size:
2579 1401 979 455 424 400 183 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3368 4612 5477 5775 3368
Size: 1244 865 298 4080
Fragments arranged by size:
4080 1244 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2520 2775 5150 882
Size: 617 1021 255 2375 2219
Fragments arranged by size:
2375 2219 1021 617 255
BsaAI yAC'GTr
Cuts at: 493 1204 2470 3247 4278 493
Size: 711 1266 777 1031 2702
Fragments arranged by size:
2702 1266 1031 777 711
BsaBI GATnn'nnATC
Cuts at: 6481 6481
Size: 6487
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2171 2348 2426 4294
Size: 53 83 186 936 638 177 78 1868
Cuts at: 4294 4676 5311 275
Size: 382 635 1451
Fragments arranged by size:
1868 1451 936 638 635 382 186 177
83 78 53
BsaWI w'CCGG_w
Cuts at: 2719 2913 4920 5318 5592 5739 6150 2719
Size: 194 2007 398 274 147 411 3056
Fragments arranged by size:
3056 2007 411 398 274 194 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2088 2118 3575 3605 1178
Size: 30 165 30 685 30 1457 30 4060
Fragments arranged by size:
4060 1457 685 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3014 3045 1186
Size: 1828 31 4628
Fragments arranged by size:
4628 1828 31
BseYI C'CCAG_C
Cuts at: 2312 2449 5690 6186 2312
Size: 137 3241 496 2613
Fragments arranged by size:
3241 2613 496 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3227 1163
Size: 573 1491 4423
Fragments arranged by size:
4423 1491 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2058 2265 3937 4698 5726 1540
Size: 275 186 57 207 1672 761 1028 2301
Fragments arranged by size:
2301 1672 1028 761 275 207 186 57
BsiHKAI G_wGCw'C
Cuts at: 2913 3578 4548 4633 5704 2913
Size: 665 970 85 1071 3696
Fragments arranged by size:
3696 1071 970 665 85
BsmI GAATG_Cn'
Cuts at: 2861 2973 3998 4091 2861
Size: 112 1025 93 5257
Fragments arranged by size:
5257 1025 112 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 2457 2913 3405 3460 3578 3593
Size: 399 397 270 456 492 55 118 15
Cuts at: 3593 3806 4548 4633 5704 1391
Size: 213 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 492 456 399 397 270
213 118 85 55 15
BspEI T'CCGG_A
Cuts at: 2719 6150 2719
Size: 3431 3056
BspHI T'CATG_A
Cuts at: 4376 6106 4376
Size: 1730 4757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2211 2718 3642 3775 3842 844
Size: 1367 507 924 133 67 3489
Fragments arranged by size:
3489 1367 924 507 133 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2088 2275 2954 4374 1394
Size: 429 265 187 679 1420 3507
Fragments arranged by size:
3507 1420 679 429 265 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 4982 5156 65
Size: 1183 3734 174 1396
Fragments arranged by size:
3734 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2385 3310 5131 5318 1700
Size: 110 575 925 1821 187 2869
Fragments arranged by size:
2869 1821 925 575 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6487
BssHII G'CGCG_C
Cuts at: 1925 2114 2116 2283 2307 1925
Size: 189 2 167 24 6105
Fragments arranged by size:
6105 189 167 24 2
BssSI C'ACGA_G
Cuts at: 3641 4547 5559 3641
Size: 906 1012 4569
Fragments arranged by size:
4569 1012 906
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3637 794
Size: 2843 3644
BstBI TT'CG_AA
Cuts at: 5291 5291
Size: 6487
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6487
BstXI CCAn_nnnn'nTGG
Cuts at: 2641 3306 3475 2641
Size: 665 169 5653
Fragments arranged by size:
5653 665 169
BstYI r'GATC_y
Cuts at: 1972 3291 3817 4184 4569 4586 6038 6124 6136
Size: 1319 526 367 385 17 1452 86 12
Cuts at: 6136 6482 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1319 526 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2248 2694 2987 3255 3426 513
Size: 552 1183 446 293 268 171 3574
Fragments arranged by size:
3574 1183 552 446 293 268 171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5748
Fragments arranged by size:
5748 683 56
BtsI GCAGTG_nn'
Cuts at: 2341 3331 4003 4797 4817 2341
Size: 990 672 794 20 4011
Fragments arranged by size:
4011 990 794 672 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6487
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6452
DraI TTT'AAA
Cuts at: 3791 3791
Size: 6487
DraIII CAC_nnn'GTG
Cuts at: 2215 2215
Size: 6487
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3563 5494 809
Size: 507 2247 1931 1802
Fragments arranged by size:
2247 1931 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2262 2319 2361
Size: 54 1425 50 275 18 432 57 42
Cuts at: 2361 2697 3308 3619 3934 4823 6452 8
Size: 336 611 311 315 889 1629 43
Fragments arranged by size:
1629 1425 889 611 432 336 315 311
275 57 54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 2262 3934 1537
Size: 275 450 1672 4090
Fragments arranged by size:
4090 1672 450 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2600 2991 3566 4417 1353
Size: 1247 391 575 851 3423
Fragments arranged by size:
3423 1247 851 575 391
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2109 5074 5448 5594 387
Size: 1064 138 520 2965 374 146 1280
Fragments arranged by size:
2965 1280 1064 520 374 146 138
EcoICRI GAG'CTC
Cuts at: 2911 2911
Size: 6487
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2017 2218 2509 2642 3042 3497 3563
Size: 393 385 201 291 133 400 455 66
Cuts at: 3563 3746 4550 5147 5934 6202 1239
Size: 183 804 597 787 268 1524
Fragments arranged by size:
1524 804 787 597 455 400 393 385
291 268 201 183 133 66
EcoNI CCTnn'n_nnAGG
Cuts at: 3057 3057
Size: 6487
EcoO109I rG'GnC_Cy
Cuts at: 2412 2453 2821 3207 3401 2412
Size: 41 368 386 194 5498
Fragments arranged by size:
5498 386 368 194 41
EcoRI G'AATT_C
Cuts at: 3796 4174 3796
Size: 378 6109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2614 2614
Size: 6487
FseI GG_CCGG'CC
Cuts at: 2389 2389
Size: 6487
FspI TGC'GCA
Cuts at: 2165 4993 2165
Size: 2828 3659
HaeII r_GCGC'y
Cuts at: 1678 2162 2174 2351 2373 2429 5634 1678
Size: 484 12 177 22 56 3205 2531
Fragments arranged by size:
3205 2531 484 177 56 22 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2938 2970 3040 3072 5128
Size: 32 135 32 1366 32 70 32 2056
Cuts at: 5128 5160 5202 5234 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2056 1366 135 70 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3280 3831 4077 669
Size: 988 281 1342 551 246 3079
Fragments arranged by size:
3079 1342 988 551 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6487
HpaI GTT'AAC
Cuts at: 4077 4077
Size: 6487
KasI G'GCGC_C
Cuts at: 2170 2347 2425 2170
Size: 177 78 6232
Fragments arranged by size:
6232 177 78
KpnI G_GTAC'C
Cuts at: 3505 3812 3505
Size: 307 6180
MfeI C'AATT_G
Cuts at: 4086 4086
Size: 6487
MluI A'CGCG_T
Cuts at: 6363 6363
Size: 6487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5601 5785 1305
Size: 4296 184 2007
Fragments arranged by size:
4296 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3621 10
Size: 54 1425 343 1789 2876
Fragments arranged by size:
2876 1789 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2468 3608 4445 4804 4963 518
Size: 648 1302 1140 837 359 159 2042
Fragments arranged by size:
2042 1302 1140 837 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2250 2301 2404 3903 4580 5728 5973 1606
Size: 644 51 103 1499 677 1148 245 2120
Fragments arranged by size:
2120 1499 1148 677 644 245 103 51
NaeI GCC'GGC
Cuts at: 2387 3312 2387
Size: 925 5562
NarI GG'CG_CC
Cuts at: 2171 2348 2426 2171
Size: 177 78 6232
Fragments arranged by size:
6232 177 78
NcoI C'CATG_G
Cuts at: 513 1065 2987 513
Size: 552 1922 4013
Fragments arranged by size:
4013 1922 552
NdeI CA'TA_TG
Cuts at: 387 2809 387
Size: 2422 4065
NgoMIV G'CCGG_C
Cuts at: 2385 3310 2385
Size: 925 5562
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6487
NotI GC'GGCC_GC
Cuts at: 3934 3934
Size: 6487
NspI r_CATG'y
Cuts at: 1132 1229 2755 3613 3845 5390 1132
Size: 97 1526 858 232 1545 2229
Fragments arranged by size:
2229 1545 1526 858 232 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6487
PciI A'CATG_T
Cuts at: 5386 5386
Size: 6487
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2449 3397 1108
Size: 1341 948 4198
Fragments arranged by size:
4198 1341 948
PmeI GTTT'AAAC
Cuts at: 3791 3791
Size: 6487
PmlI CAC'GTG
Cuts at: 3247 3247
Size: 6487
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4556 4588 6069 6101 4556
Size: 32 1481 32 4942
Fragments arranged by size:
4942 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2821 3207 2821
Size: 386 6101
PshAI GACnn'nnGTC
Cuts at: 1613 2054 1613
Size: 441 6046
PsiI TTA'TAA
Cuts at: 4057 4057
Size: 6487
PspOMI G'GGCC_C
Cuts at: 2183 2453 3401 2183
Size: 270 948 5269
Fragments arranged by size:
5269 948 270
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2527 2559 2527
Size: 32 6455
PstI C_TGCA'G
Cuts at: 830 2306 3745 3839 830
Size: 1476 1439 94 3478
Fragments arranged by size:
3478 1476 1439 94
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6487
PvuII CAG'CTG
Cuts at: 2301 2301
Size: 6487
SacI G_AGCT'C
Cuts at: 2913 2913
Size: 6487
SacII CC_GC'GG
Cuts at: 2251 2251
Size: 6487
SalI G'TCGA_C
Cuts at: 1936 3829 1936
Size: 1893 4594
SapI GCTCTTCn'nnn_
Cuts at: 3566 3566
Size: 6487
SbfI CC_TGCA'GG
Cuts at: 3839 3839
Size: 6487
ScaI AGT'ACT
Cuts at: 2480 4735 6447 2480
Size: 2255 1712 2520
Fragments arranged by size:
2520 2255 1712
SexAI A'CCwGG_T
Cuts at: 2442 2442
Size: 6487
SfcI C'TryA_G
Cuts at: 826 1045 2302 3220 3741 3835 4970 5651 5842
Size: 219 1257 918 521 94 1135 681 191
Cuts at: 5842 826
Size: 1471
Fragments arranged by size:
1471 1257 1135 918 681 521 219 191
94
SfiI GGCCn_nnn'nGGCC
Cuts at: 2250 2250
Size: 6487
SfoI GGC'GCC
Cuts at: 2172 2349 2427 2172
Size: 177 78 6232
Fragments arranged by size:
6232 177 78
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6487
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6487
SmaI CCC'GGG
Cuts at: 1872 2270 3588 3814 1872
Size: 398 1318 226 4545
Fragments arranged by size:
4545 1318 398 226
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3383 4591 5492 5754 820
Size: 197 926 1440 1208 901 262 1553
Fragments arranged by size:
1553 1440 1208 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4278 493
Size: 3785 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6487
SphI G_CATG'C
Cuts at: 1132 3613 3845 1132
Size: 2481 232 3774
Fragments arranged by size:
3774 2481 232
SspI AAT'ATT
Cuts at: 5 52 2927 4411 5
Size: 47 2875 1484 2081
Fragments arranged by size:
2875 2081 1484 47
StuI AGG'CCT
Cuts at: 1517 3305 1517
Size: 1788 4699
StyI C'CwwG_G
Cuts at: 513 1065 2987 3341 3396 3505 3926 513
Size: 552 1922 354 55 109 421 3074
Fragments arranged by size:
3074 1922 552 421 354 109 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2044 3036 3283 4514 4531 4684 4869
Size: 1255 464 992 247 1231 17 153 185
Cuts at: 4869 5314 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1231 992 464 445 247 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2478 2528 4733
Size: 274 80 33 51 1443 501 50 2205
Cuts at: 4733 6445 96
Size: 1712 138
Fragments arranged by size:
2205 1712 1443 501 274 138 80 51
50 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2201 2233 2741 2773 6447 6479 2201
Size: 32 508 32 3674 32 2209
Fragments arranged by size:
3674 2209 508 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3677 1316
Size: 2361 4126
XbaI T'CTAG_A
Cuts at: 3823 3823
Size: 6487
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3303 3303
Size: 6487
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6487
XmaI C'CCGG_G
Cuts at: 1870 2268 3586 3812 1870
Size: 398 1318 226 4545
Fragments arranged by size:
4545 1318 398 226
XmnI GAAnn'nnTTC
Cuts at: 4616 4616
Size: 6487
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4295 276
Size: 53 83 186 936 2761 2468
Fragments arranged by size:
2761 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoRV FspAI NruI NsiI
PacI PfoI PspXI RsrII SanDI SrfI SwaI