Restriction map for
pFN21AE4530
(Circular) (Six-base) MAPSORT of: pFN21AE4530.seq Check: 6350 from: 1 to: 6481
pFN21AE4530 6481 bp
With 182 enzymes: SgfI *
March 28, 2012 14:09 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4291 278
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3824 1937
Size: 1887 4594
Acc65I G'GTAC_C
Cuts at: 2636 3802 2636
Size: 1166 5315
AclI AA'CG_TT
Cuts at: 1563 4608 4981 1563
Size: 3045 373 3063
Fragments arranged by size:
3063 3045 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2108 2642 2648 3221 3353 3396 3492 3650
Size: 476 534 6 573 132 43 96 158
Cuts at: 3650 4544 5928 6196 1632
Size: 894 1384 268 1917
Fragments arranged by size:
1917 1384 894 573 534 476 268 158
132 96 43 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6284
AflIII A'CryG_T
Cuts at: 1203 3481 5380 6357 1203
Size: 2278 1899 977 1327
Fragments arranged by size:
2278 1899 1327 977
AgeI A'CCGG_T
Cuts at: 5312 5312
Size: 6481
AhdI GACnn_n'nnGTC
Cuts at: 5210 5210
Size: 6481
AleI CACnn'nnGTG
Cuts at: 1166 2381 1166
Size: 1215 5266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6481
AlwNI CAG_nnn'CTG
Cuts at: 2676 3061 3631 4224 5796 2676
Size: 385 570 593 1572 3361
Fragments arranged by size:
3361 1572 593 570 385
ApaLI G'TGCA_C
Cuts at: 4538 5694 4538
Size: 1156 5325
ApoI r'AATT_y
Cuts at: 1493 2687 2786 3279 3790 4016 4168 6277 1493
Size: 1194 99 493 511 226 152 2109 1697
Fragments arranged by size:
2109 1697 1194 511 493 226 152 99
AseI AT'TA_AT
Cuts at: 160 1032 5035 160
Size: 872 4003 1606
Fragments arranged by size:
4003 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
AvaI C'yCGr_G
Cuts at: 1870 1943 2753 3806 1870
Size: 73 810 1053 4545
Fragments arranged by size:
4545 1053 810 73
BamHI G'GATC_C
Cuts at: 3811 4178 3811
Size: 367 6114
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2636 3802 5257 618
Size: 325 217 627 849 1166 1455 1842
Fragments arranged by size:
1842 1455 1166 849 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2210 3800 1391
Size: 819 1590 4072
Fragments arranged by size:
4072 1590 819
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3097 928
Size: 971 1198 4312
Fragments arranged by size:
4312 1198 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2362 2396 4672 4706 1903
Size: 34 425 34 2276 34 3678
Fragments arranged by size:
3678 2276 425 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2690 3360 4373 5583 2690
Size: 670 1013 1210 3588
Fragments arranged by size:
3588 1210 1013 670
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6481
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5092 136
Size: 107 122 71 631 605 3420 1525
Fragments arranged by size:
3420 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6476 6476
Size: 6481
BlpI GC'TnA_GC
Cuts at: 3898 3898
Size: 6481
Bme1580I G_kGCm'C
Cuts at: 1790 2256 4542 5698 1790
Size: 466 2286 1156 2573
Fragments arranged by size:
2573 2286 1156 466
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6481
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4212 5170 442
Size: 416 53 83 399 24 2795 958 1753
Fragments arranged by size:
2795 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3038 3416 3725 5141 1239
Size: 1799 378 309 1416 2579
Fragments arranged by size:
2579 1799 1416 378 309
Bpu10I CC'TnA_GC
Cuts at: 2369 3226 2369
Size: 857 5624
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4606 5471 5769 4606
Size: 865 298 5318
Fragments arranged by size:
5318 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5144 882
Size: 617 3645 2219
Fragments arranged by size:
3645 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4272 493
Size: 711 3068 2702
Fragments arranged by size:
3068 2702 711
BsaBI GATnn'nnATC
Cuts at: 2467 6475 2467
Size: 4008 2473
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4288 4670 5305 275
Size: 53 83 186 936 2755 382 635 1451
Fragments arranged by size:
2755 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2682 4914 5312 5586 5733 6144 2682
Size: 2232 398 274 147 411 3019
Fragments arranged by size:
3019 2232 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3723 3753 1178
Size: 30 165 30 2320 30 3906
Fragments arranged by size:
3906 2320 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3583 1186
Size: 2397 4084
BseYI C'CCAG_C
Cuts at: 2748 3162 5684 6180 2748
Size: 414 2522 496 3049
Fragments arranged by size:
3049 2522 496 414
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 3053 3056 3483 1163
Size: 573 501 816 3 427 4161
Fragments arranged by size:
4161 816 573 501 427 3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3931 4692 5720 1540
Size: 275 186 1930 761 1028 2301
Fragments arranged by size:
2301 1930 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4542 4627 5698 4542
Size: 85 1071 5325
Fragments arranged by size:
5325 1071 85
BsmI GAATG_Cn'
Cuts at: 3992 4085 3992
Size: 93 6388
BsmBI CGTCTCn'nnnn_
Cuts at: 3311 3748 3311
Size: 437 6044
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2210 2256 3800 4542 4627 5698 1391
Size: 399 420 46 1544 742 85 1071 2174
Fragments arranged by size:
2174 1544 1071 742 420 399 85 46
BspEI T'CCGG_A
Cuts at: 2682 6144 2682
Size: 3462 3019
BspHI T'CATG_A
Cuts at: 4370 6100 4370
Size: 1730 4751
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2673 3078 3836 844
Size: 1829 405 758 3489
Fragments arranged by size:
3489 1829 758 405
BsrBI CCG'CTC
Cuts at: 1394 1823 4368 1394
Size: 429 2545 3507
Fragments arranged by size:
3507 2545 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4976 5150 65
Size: 1183 3728 174 1396
Fragments arranged by size:
3728 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5125 5312 1700
Size: 110 3315 187 2869
Fragments arranged by size:
3315 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2385 96
Size: 2289 4192
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6481
BssSI C'ACGA_G
Cuts at: 4541 5553 4541
Size: 1012 5469
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3529 794
Size: 2735 3746
BstBI TT'CG_AA
Cuts at: 3287 5285 3287
Size: 1998 4483
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6481
BstYI r'GATC_y
Cuts at: 1972 2016 2091 2346 2926 3736 3811 4178 4563
Size: 44 75 255 580 810 75 367 385
Cuts at: 4563 4580 6032 6118 6130 6476 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 810 580 385 367 346 255
86 75 75 44 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2211 513
Size: 552 939 207 4783
Fragments arranged by size:
4783 939 552 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5742
Fragments arranged by size:
5742 683 56
BtsI GCAGTG_nn'
Cuts at: 2926 2991 3997 4791 4811 2926
Size: 65 1006 794 20 4596
Fragments arranged by size:
4596 1006 794 65 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6481
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6446
DraI TTT'AAA
Cuts at: 3785 3785
Size: 6481
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5488 809
Size: 507 4172 1802
Fragments arranged by size:
4172 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2358 3928 4817
Size: 54 1425 50 275 18 528 1570 889
Cuts at: 4817 6446 8
Size: 1629 43
Fragments arranged by size:
1629 1570 1425 889 528 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3928 1537
Size: 275 2116 4090
Fragments arranged by size:
4090 2116 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2766 3372 4411 1353
Size: 1413 606 1039 3423
Fragments arranged by size:
3423 1413 1039 606
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5068 5442 5588 387
Size: 1064 138 3479 374 146 1280
Fragments arranged by size:
3479 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2642 2648 3038 3221 3353 3396
Size: 393 476 534 6 390 183 132 43
Cuts at: 3396 3416 3492 3650 3725 4544 5141 5928 6196
Size: 20 76 158 75 819 597 787 268
Cuts at: 6196 1239
Size: 1524
Fragments arranged by size:
1524 819 787 597 534 476 393 390
268 183 158 132 76 75 43 20
6
EcoO109I rG'GnC_Cy
Cuts at: 3648 3648
Size: 6481
EcoRI G'AATT_C
Cuts at: 3790 4168 3790
Size: 378 6103
FspI TGC'GCA
Cuts at: 4987 4987
Size: 6481
HaeII r_GCGC'y
Cuts at: 1678 5628 1678
Size: 3950 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2059 2091 2574 2606 2664
Size: 32 135 32 487 32 483 32 58
Cuts at: 2664 2696 3209 3241 3559 3591 3640 3672 3721
Size: 32 513 32 318 32 49 32 49
Cuts at: 3721 3753 5122 5154 5196 5228 1373
Size: 32 1369 32 42 32 2626
Fragments arranged by size:
2626 1369 513 487 483 318 135 58
49 49 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2869 3825 4071 669
Size: 988 281 931 956 246 3079
Fragments arranged by size:
3079 988 956 931 281 246
HindIII A'AGCT_T
Cuts at: 748 2741 748
Size: 1993 4488
HpaI GTT'AAC
Cuts at: 4071 4071
Size: 6481
KpnI G_GTAC'C
Cuts at: 2640 3806 2640
Size: 1166 5315
MfeI C'AATT_G
Cuts at: 2130 4080 2130
Size: 1950 4531
MluI A'CGCG_T
Cuts at: 6357 6357
Size: 6481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5595 5779 1305
Size: 4290 184 2007
Fragments arranged by size:
4290 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4659
Fragments arranged by size:
4659 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 4439 4798 4957 518
Size: 648 1215 2058 359 159 2042
Fragments arranged by size:
2058 2042 1215 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2510 2981 3037 3897 4574 5722 5967 1606
Size: 904 471 56 860 677 1148 245 2120
Fragments arranged by size:
2120 1148 904 860 677 471 245 56
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4990
Fragments arranged by size:
4990 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6481
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6481
NotI GC'GGCC_GC
Cuts at: 3928 3928
Size: 6481
NspI r_CATG'y
Cuts at: 1132 1229 3771 3839 5384 1132
Size: 97 2542 68 1545 2229
Fragments arranged by size:
2542 2229 1545 97 68
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6481
PciI A'CATG_T
Cuts at: 5380 5380
Size: 6481
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6481
PfoI T'CCnGG_A
Cuts at: 2342 3054 2342
Size: 712 5769
PmeI GTTT'AAAC
Cuts at: 3785 3785
Size: 6481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4550 4582 6063 6095 4550
Size: 32 1481 32 4936
Fragments arranged by size:
4936 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3648 3648
Size: 6481
PshAI GACnn'nnGTC
Cuts at: 1613 2903 1613
Size: 1290 5191
PsiI TTA'TAA
Cuts at: 4051 4051
Size: 6481
PstI C_TGCA'G
Cuts at: 830 2986 3390 3833 830
Size: 2156 404 443 3478
Fragments arranged by size:
3478 2156 443 404
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6481
PvuII CAG'CTG
Cuts at: 2510 2981 3037 2510
Size: 471 56 5954
Fragments arranged by size:
5954 471 56
SalI G'TCGA_C
Cuts at: 1936 3823 1936
Size: 1887 4594
SapI GCTCTTCn'nnn_
Cuts at: 2766 2766
Size: 6481
SbfI CC_TGCA'GG
Cuts at: 3833 3833
Size: 6481
ScaI AGT'ACT
Cuts at: 3344 4729 6441 3344
Size: 1385 1712 3384
Fragments arranged by size:
3384 1712 1385
SfcI C'TryA_G
Cuts at: 826 1045 2982 3386 3829 4964 5645 5836 826
Size: 219 1937 404 443 1135 681 191 1471
Fragments arranged by size:
1937 1471 1135 681 443 404 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6481
SmaI CCC'GGG
Cuts at: 1872 3808 1872
Size: 1936 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2753 4585 5486 5748 820
Size: 197 926 810 1832 901 262 1553
Fragments arranged by size:
1832 1553 926 901 810 262 197
SnaBI TAC'GTA
Cuts at: 493 4272 493
Size: 3779 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6481
SphI G_CATG'C
Cuts at: 1132 3839 1132
Size: 2707 3774
SspI AAT'ATT
Cuts at: 5 52 4405 5
Size: 47 4353 2081
Fragments arranged by size:
4353 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6481
StyI C'CwwG_G
Cuts at: 513 1065 2004 2958 3405 3920 513
Size: 552 939 954 447 515 3074
Fragments arranged by size:
3074 954 939 552 515 447
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4508 4525 4678 4863 5308 325
Size: 1255 2928 17 153 185 445 1498
Fragments arranged by size:
2928 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2385 2430 3342
Size: 274 80 33 51 1443 408 45 912
Cuts at: 3342 4727 6439 96
Size: 1385 1712 138
Fragments arranged by size:
1712 1443 1385 912 408 274 138 80
51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2233 2265 2442 2474 2918 2950 6441 6473 2233
Size: 32 177 32 444 32 3491 32 2241
Fragments arranged by size:
3491 2241 444 177 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3620 1316
Size: 2304 4177
XbaI T'CTAG_A
Cuts at: 2881 3256 3817 2881
Size: 375 561 5545
Fragments arranged by size:
5545 561 375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2538 2538
Size: 6481
XhoI C'TCGA_G
Cuts at: 1943 2753 1943
Size: 810 5671
XmaI C'CCGG_G
Cuts at: 1870 3806 1870
Size: 1936 4545
XmnI GAAnn'nnTTC
Cuts at: 2690 3283 3381 4610 2690
Size: 593 98 1229 4561
Fragments arranged by size:
4561 1229 593 98
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4289 276
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI