Restriction map for
pFN21AE4527
(Circular) (Six-base) MAPSORT of: pFN21AE4527.seq Check: 1205 from: 1 to: 6433
pFN21AE4527 6433 bp
With 182 enzymes: SgfI *
March 28, 2012 14:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3080 3080
Size: 6433
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4243 278
Size: 53 83 186 936 2707 2468
Fragments arranged by size:
2707 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2094 3776 1937
Size: 157 1682 4594
Fragments arranged by size:
4594 1682 157
Acc65I G'GTAC_C
Cuts at: 3754 3754
Size: 6433
AclI AA'CG_TT
Cuts at: 1563 4560 4933 1563
Size: 2997 373 3063
Fragments arranged by size:
3063 2997 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2140 4496 5880 6148 1632
Size: 508 2356 1384 268 1917
Fragments arranged by size:
2356 1917 1384 508 268
AfeI AGC'GCT
Cuts at: 2147 2147
Size: 6433
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6236
AflIII A'CryG_T
Cuts at: 1203 5332 6309 1203
Size: 4129 977 1327
Fragments arranged by size:
4129 1327 977
AgeI A'CCGG_T
Cuts at: 5264 5264
Size: 6433
AhdI GACnn_n'nnGTC
Cuts at: 5162 5162
Size: 6433
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6433
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6433
AlwNI CAG_nnn'CTG
Cuts at: 3095 3728 4176 5748 3095
Size: 633 448 1572 3780
Fragments arranged by size:
3780 1572 633 448
ApaLI G'TGCA_C
Cuts at: 4490 5646 4490
Size: 1156 5277
ApoI r'AATT_y
Cuts at: 1493 2281 2812 3337 3543 3581 3742 3968 4120
Size: 788 531 525 206 38 161 226 152
Cuts at: 4120 6229 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 788 531 525 226 206 161
152 38
AseI AT'TA_AT
Cuts at: 160 1032 2310 4987 160
Size: 872 1278 2677 1606
Fragments arranged by size:
2677 1606 1278 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6433
AvaI C'yCGr_G
Cuts at: 1870 1943 3758 1870
Size: 73 1815 4545
Fragments arranged by size:
4545 1815 73
AvrII C'CTAG_G
Cuts at: 2173 2173
Size: 6433
BamHI G'GATC_C
Cuts at: 3763 4130 3763
Size: 367 6066
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3754 5209 618
Size: 325 217 627 1967 1455 1842
Fragments arranged by size:
1967 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2199 3752 1391
Size: 808 1553 4072
Fragments arranged by size:
4072 1553 808
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5462
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4624 4658 1903
Size: 34 2687 34 3678
Fragments arranged by size:
3678 2687 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4325 5535 4325
Size: 1210 5223
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6433
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5044 136
Size: 107 122 71 631 605 3372 1525
Fragments arranged by size:
3372 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2122 6428 2122
Size: 4306 2127
BlpI GC'TnA_GC
Cuts at: 3850 3850
Size: 6433
Bme1580I G_kGCm'C
Cuts at: 1790 4494 5650 1790
Size: 2704 1156 2573
Fragments arranged by size:
2704 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3328 1318
Size: 2010 4423
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2644 2730 3147
Size: 416 53 83 399 24 1227 86 417
Cuts at: 3147 4164 5122 442
Size: 1017 958 1753
Fragments arranged by size:
1753 1227 1017 958 417 416 399 86
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 5093 1239
Size: 968 2886 2579
Fragments arranged by size:
2886 2579 968
Bpu10I CC'TnA_GC
Cuts at: 3225 3225
Size: 6433
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3402 3586 4558 5423 5721 3402
Size: 184 972 865 298 4114
Fragments arranged by size:
4114 972 865 298 184
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3515 5096 882
Size: 617 2016 1581 2219
Fragments arranged by size:
2219 2016 1581 617
BsaAI yAC'GTr
Cuts at: 493 1204 4224 493
Size: 711 3020 2702
Fragments arranged by size:
3020 2702 711
BsaBI GATnn'nnATC
Cuts at: 6427 6427
Size: 6433
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4240 4622 5257 275
Size: 53 83 186 936 2707 382 635 1451
Fragments arranged by size:
2707 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4866 5264 5538 5685 6096 4866
Size: 398 274 147 411 5203
Fragments arranged by size:
5203 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3603 3633 1178
Size: 30 165 30 2200 30 3978
Fragments arranged by size:
3978 2200 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2283 2369 2734 1186
Size: 1097 86 365 4885
Fragments arranged by size:
4885 1097 365 86
BseYI C'CCAG_C
Cuts at: 2786 2915 3525 5636 6132 2786
Size: 129 610 2111 496 3087
Fragments arranged by size:
3087 2111 610 496 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2985 1163
Size: 573 1249 4611
Fragments arranged by size:
4611 1249 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3883 4644 5672 1540
Size: 275 186 1882 761 1028 2301
Fragments arranged by size:
2301 1882 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3002 4494 4579 5650 3002
Size: 1492 85 1071 3785
Fragments arranged by size:
3785 1492 1071 85
BsmI GAATG_Cn'
Cuts at: 3944 4037 3944
Size: 93 6340
BsmBI CGTCTCn'nnnn_
Cuts at: 2012 2012
Size: 6433
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2199 3002 3752 4494 4579 5650 1391
Size: 399 409 803 750 742 85 1071 2174
Fragments arranged by size:
2174 1071 803 750 742 409 399 85
BspEI T'CCGG_A
Cuts at: 6096 6096
Size: 6433
BspHI T'CATG_A
Cuts at: 4322 6052 4322
Size: 1730 4703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3080 3568 3736 3788 844
Size: 2236 488 168 52 3489
Fragments arranged by size:
3489 2236 488 168 52
BsrBI CCG'CTC
Cuts at: 1394 1823 4320 1394
Size: 429 2497 3507
Fragments arranged by size:
3507 2497 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4928 5102 65
Size: 1183 3680 174 1396
Fragments arranged by size:
3680 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5077 5264 1700
Size: 110 3267 187 2869
Fragments arranged by size:
3267 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6433
BssHII G'CGCG_C
Cuts at: 1925 2217 2219 1925
Size: 292 2 6139
Fragments arranged by size:
6139 292 2
BssSI C'ACGA_G
Cuts at: 4493 5505 4493
Size: 1012 5421
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3095 794
Size: 2301 4132
BstBI TT'CG_AA
Cuts at: 5237 5237
Size: 6433
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6433
BstYI r'GATC_y
Cuts at: 1972 2122 2443 3763 4130 4515 4532 5984 6070
Size: 150 321 1320 367 385 17 1452 86
Cuts at: 6070 6082 6428 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1320 385 367 346 321 150
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4942
Fragments arranged by size:
4942 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5694
Fragments arranged by size:
5694 683 56
BtsI GCAGTG_nn'
Cuts at: 2631 3949 4743 4763 2631
Size: 1318 794 20 4301
Fragments arranged by size:
4301 1318 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6433
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6398
DraI TTT'AAA
Cuts at: 3737 3737
Size: 6433
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5440 809
Size: 507 4124 1802
Fragments arranged by size:
4124 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3880 4769 6398
Size: 54 1425 50 275 18 2050 889 1629
Cuts at: 6398 8
Size: 43
Fragments arranged by size:
2050 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3880 1537
Size: 275 2068 4090
Fragments arranged by size:
4090 2068 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2256 2399 2532 3046 3175 3192 4363 1353
Size: 903 143 133 514 129 17 1171 3423
Fragments arranged by size:
3423 1171 903 514 143 133 129 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2129 5020 5394 5540 387
Size: 1064 138 540 2891 374 146 1280
Fragments arranged by size:
2891 1280 1064 540 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2140 2207 4496 5093 5880 6148 1239
Size: 393 508 67 2289 597 787 268 1524
Fragments arranged by size:
2289 1524 787 597 508 393 268 67
EcoO109I rG'GnC_Cy
Cuts at: 2166 2166
Size: 6433
EcoRI G'AATT_C
Cuts at: 2281 3742 4120 2281
Size: 1461 378 4594
Fragments arranged by size:
4594 1461 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3221 3221
Size: 6433
FspI TGC'GCA
Cuts at: 4939 4939
Size: 6433
HaeII r_GCGC'y
Cuts at: 1678 2149 5580 1678
Size: 471 3431 2531
Fragments arranged by size:
3431 2531 471
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2097 2129 2741 2773 3443
Size: 32 135 32 525 32 612 32 670
Cuts at: 3443 3475 5074 5106 5148 5180 1373
Size: 32 1599 32 42 32 2626
Fragments arranged by size:
2626 1599 670 612 525 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2095 3777 4023 669
Size: 988 281 157 1682 246 3079
Fragments arranged by size:
3079 1682 988 281 246 157
HindIII A'AGCT_T
Cuts at: 748 3443 748
Size: 2695 3738
HpaI GTT'AAC
Cuts at: 4023 4023
Size: 6433
KpnI G_GTAC'C
Cuts at: 3758 3758
Size: 6433
MfeI C'AATT_G
Cuts at: 4032 4032
Size: 6433
MluI A'CGCG_T
Cuts at: 6309 6309
Size: 6433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3403 5547 5731 1305
Size: 2098 2144 184 2007
Fragments arranged by size:
2144 2098 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4611
Fragments arranged by size:
4611 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4391 4750 4909 518
Size: 648 3225 359 159 2042
Fragments arranged by size:
3225 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3410 3849 4526 5674 5919 1606
Size: 1804 439 677 1148 245 2120
Fragments arranged by size:
2120 1804 1148 677 439 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4942
Fragments arranged by size:
4942 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6433
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6433
NotI GC'GGCC_GC
Cuts at: 3880 3880
Size: 6433
NspI r_CATG'y
Cuts at: 1132 1229 3241 3791 5336 1132
Size: 97 2012 550 1545 2229
Fragments arranged by size:
2229 2012 1545 550 97
PasI CC'CwG_GG
Cuts at: 1348 3068 1348
Size: 1720 4713
PciI A'CATG_T
Cuts at: 5332 5332
Size: 6433
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6433
PmeI GTTT'AAAC
Cuts at: 3737 3737
Size: 6433
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2638 2670 2722 2754 4502 4534 6015 6047 2638
Size: 32 52 32 1748 32 1481 32 3024
Fragments arranged by size:
3024 1748 1481 52 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3387 1613
Size: 1774 4659
PsiI TTA'TAA
Cuts at: 4003 4003
Size: 6433
PstI C_TGCA'G
Cuts at: 830 2878 3091 3785 830
Size: 2048 213 694 3478
Fragments arranged by size:
3478 2048 694 213
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6433
PvuII CAG'CTG
Cuts at: 3410 3410
Size: 6433
SalI G'TCGA_C
Cuts at: 1936 2093 3775 1936
Size: 157 1682 4594
Fragments arranged by size:
4594 1682 157
SbfI CC_TGCA'GG
Cuts at: 3785 3785
Size: 6433
ScaI AGT'ACT
Cuts at: 4681 6393 4681
Size: 1712 4721
SfcI C'TryA_G
Cuts at: 826 1045 2286 2874 3087 3385 3781 4916 5597
Size: 219 1241 588 213 298 396 1135 681
Cuts at: 5597 5788 826
Size: 191 1471
Fragments arranged by size:
1471 1241 1135 681 588 396 298 219
213 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6433
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6433
SmaI CCC'GGG
Cuts at: 1872 3760 1872
Size: 1888 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3417 3565 4537 5438 5700 820
Size: 197 926 1474 148 972 901 262 1553
Fragments arranged by size:
1553 1474 972 926 901 262 197 148
SnaBI TAC'GTA
Cuts at: 493 4224 493
Size: 3731 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6433
SphI G_CATG'C
Cuts at: 1132 3241 3791 1132
Size: 2109 550 3774
Fragments arranged by size:
3774 2109 550
SspI AAT'ATT
Cuts at: 5 52 2387 3504 4357 5
Size: 47 2335 1117 853 2081
Fragments arranged by size:
2335 2081 1117 853 47
StuI AGG'CCT
Cuts at: 1517 2178 3039 1517
Size: 661 861 4911
Fragments arranged by size:
4911 861 661
StyI C'CwwG_G
Cuts at: 513 1065 2004 2173 3872 513
Size: 552 939 169 1699 3074
Fragments arranged by size:
3074 1699 939 552 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2513 3388 4460 4477 4630 4815 5260
Size: 1255 933 875 1072 17 153 185 445
Cuts at: 5260 325
Size: 1498
Fragments arranged by size:
1498 1255 1072 933 875 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2617 3719 4679
Size: 274 80 33 51 1443 640 1102 960
Cuts at: 4679 6391 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1102 960 640 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2692 2724 6393 6425 2692
Size: 32 3669 32 2700
Fragments arranged by size:
3669 2700 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6433
XbaI T'CTAG_A
Cuts at: 3769 3769
Size: 6433
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6433
XmaI C'CCGG_G
Cuts at: 1870 3758 1870
Size: 1888 4545
XmnI GAAnn'nnTTC
Cuts at: 4562 4562
Size: 6433
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4241 276
Size: 53 83 186 936 2707 2468
Fragments arranged by size:
2707 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI