Restriction map for
pFN21AE4523
(Circular) (Six-base) MAPSORT of: pFN21AE4523.seq Check: 3294 from: 1 to: 6394
pFN21AE4523 6394 bp
With 182 enzymes: SgfI *
March 28, 2012 14:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2560 3318 2560
Size: 758 5636
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4204 278
Size: 53 83 186 936 2668 2468
Fragments arranged by size:
2668 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2877 3737 1937
Size: 940 860 4594
Fragments arranged by size:
4594 940 860
Acc65I G'GTAC_C
Cuts at: 3715 3715
Size: 6394
AclI AA'CG_TT
Cuts at: 1563 4521 4894 1563
Size: 2958 373 3063
Fragments arranged by size:
3063 2958 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3223 3695 4457 5841 6109 1632
Size: 1591 472 762 1384 268 1917
Fragments arranged by size:
1917 1591 1384 762 472 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6197
AflIII A'CryG_T
Cuts at: 1203 2959 5293 6270 1203
Size: 1756 2334 977 1327
Fragments arranged by size:
2334 1756 1327 977
AgeI A'CCGG_T
Cuts at: 5225 5225
Size: 6394
AhdI GACnn_n'nnGTC
Cuts at: 5123 5123
Size: 6394
AleI CACnn'nnGTG
Cuts at: 1166 3164 1166
Size: 1998 4396
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3416 1184
Size: 2232 4162
AlwNI CAG_nnn'CTG
Cuts at: 2582 2695 2842 4137 5709 2582
Size: 113 147 1295 1572 3267
Fragments arranged by size:
3267 1572 1295 147 113
ApaLI G'TGCA_C
Cuts at: 4451 5607 4451
Size: 1156 5238
ApoI r'AATT_y
Cuts at: 1493 3703 3929 4081 6190 1493
Size: 2210 226 152 2109 1697
Fragments arranged by size:
2210 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4948 160
Size: 872 3916 1606
Fragments arranged by size:
3916 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6394
AvaI C'yCGr_G
Cuts at: 1870 1943 3051 3719 1870
Size: 73 1108 668 4545
Fragments arranged by size:
4545 1108 668 73
AvrII C'CTAG_G
Cuts at: 2026 2026
Size: 6394
BamHI G'GATC_C
Cuts at: 2212 3453 3480 3631 3724 4091 2212
Size: 1241 27 151 93 367 4515
Fragments arranged by size:
4515 1241 367 151 93 27
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2660 2855 3715 5170 618
Size: 325 217 627 873 195 860 1455 1842
Fragments arranged by size:
1842 1455 873 860 627 325 217 195
BanII G_rGCy'C
Cuts at: 1391 2034 2079 2329 2657 2913 3036 3299 3713
Size: 643 45 250 328 256 123 263 414
Cuts at: 3713 1391
Size: 4072
Fragments arranged by size:
4072 643 414 328 263 256 250 123
45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3121 3683 928
Size: 971 1222 562 3639
Fragments arranged by size:
3639 1222 971 562
BbvCI CC'TCA_GC
Cuts at: 2999 2999
Size: 6394
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4585 4619 1903
Size: 34 2648 34 3678
Fragments arranged by size:
3678 2648 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4286 5496 4286
Size: 1210 5184
BclI T'GATC_A
Cuts at: 1548 2346 3465 1548
Size: 798 1119 4477
Fragments arranged by size:
4477 1119 798
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5005 136
Size: 107 122 71 631 605 3333 1525
Fragments arranged by size:
3333 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6389 6389
Size: 6394
BlpI GC'TnA_GC
Cuts at: 2375 3811 2375
Size: 1436 4958
Bme1580I G_kGCm'C
Cuts at: 1790 2689 3405 4455 5611 1790
Size: 899 716 1050 1156 2573
Fragments arranged by size:
2573 1156 1050 899 716
BmgBI CAC'GTC
Cuts at: 1318 2650 1318
Size: 1332 5062
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2683 3391 4125
Size: 416 53 83 399 24 1266 708 734
Cuts at: 4125 5083 442
Size: 958 1753
Fragments arranged by size:
1753 1266 958 734 708 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2717 3130 3423 5054 1239
Size: 1478 413 293 1631 2579
Fragments arranged by size:
2579 1631 1478 413 293
Bpu10I CC'TnA_GC
Cuts at: 2562 2999 3293 2562
Size: 437 294 5663
Fragments arranged by size:
5663 437 294
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4519 5384 5682 4519
Size: 865 298 5231
Fragments arranged by size:
5231 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3062 5057 882
Size: 617 1563 1995 2219
Fragments arranged by size:
2219 1995 1563 617
BsaAI yAC'GTr
Cuts at: 493 1204 4185 493
Size: 711 2981 2702
Fragments arranged by size:
2981 2702 711
BsaBI GATnn'nnATC
Cuts at: 6388 6388
Size: 6394
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4201 4583 5218 275
Size: 53 83 186 936 2668 382 635 1451
Fragments arranged by size:
2668 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3419 4827 5225 5499 5646 6057 3419
Size: 1408 398 274 147 411 3756
Fragments arranged by size:
3756 1408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2274 2304 1178
Size: 30 165 30 871 30 5268
Fragments arranged by size:
5268 871 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2214 1186
Size: 1028 5366
BseYI C'CCAG_C
Cuts at: 2106 2216 3627 3645 3664 5597 6093 2106
Size: 110 1411 18 19 1933 496 2407
Fragments arranged by size:
2407 1933 1411 496 110 19 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 2507 2855 3348 1163
Size: 573 483 288 348 493 4209
Fragments arranged by size:
4209 573 493 483 348 288
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3844 4605 5633 1540
Size: 275 186 1843 761 1028 2301
Fragments arranged by size:
2301 1843 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3036 3299 3477 4455 4540 5611 3036
Size: 263 178 978 85 1071 3819
Fragments arranged by size:
3819 1071 978 263 178 85
BsmI GAATG_Cn'
Cuts at: 3905 3998 3905
Size: 93 6301
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2079 2329 2657 2689 2913 3036
Size: 399 244 45 250 328 32 224 123
Cuts at: 3036 3299 3405 3477 3713 4455 4540 5611 1391
Size: 263 106 72 236 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 328 263 250 244
236 224 123 106 85 72 45 32
BspEI T'CCGG_A
Cuts at: 3419 6057 3419
Size: 2638 3756
BspHI T'CATG_A
Cuts at: 3036 4283 6013 3036
Size: 1247 1730 3417
Fragments arranged by size:
3417 1730 1247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2560 3318 3749 844
Size: 1716 758 431 3489
Fragments arranged by size:
3489 1716 758 431
BsrBI CCG'CTC
Cuts at: 1394 1823 3050 4281 1394
Size: 429 1227 1231 3507
Fragments arranged by size:
3507 1231 1227 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3250 4889 5063 65
Size: 1183 2002 1639 174 1396
Fragments arranged by size:
2002 1639 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2892 5038 5225 1700
Size: 110 1082 2146 187 2869
Fragments arranged by size:
2869 2146 1082 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6394
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6394
BssSI C'ACGA_G
Cuts at: 3119 4454 5466 3119
Size: 1335 1012 4047
Fragments arranged by size:
4047 1335 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6394
BstBI TT'CG_AA
Cuts at: 5198 5198
Size: 6394
BstEII G'GTnAC_C
Cuts at: 1184 2602 1184
Size: 1418 4976
BstXI CCAn_nnnn'nTGG
Cuts at: 3536 3536
Size: 6394
BstYI r'GATC_y
Cuts at: 1972 2212 3453 3480 3631 3724 4091 4476 4493
Size: 240 1241 27 151 93 367 385 17
Cuts at: 4493 5945 6031 6043 6389 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1241 385 367 346 240 151
93 86 27 17 12
Bsu36I CC'TnA_GG
Cuts at: 2133 2549 3076 2133
Size: 416 527 5451
Fragments arranged by size:
5451 527 416
BtgI C'CryG_G
Cuts at: 513 1065 2004 3254 3410 3562 513
Size: 552 939 1250 156 152 3345
Fragments arranged by size:
3345 1250 939 552 156 152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5655
Fragments arranged by size:
5655 683 56
BtsI GCAGTG_nn'
Cuts at: 2927 3910 4704 4724 2927
Size: 983 794 20 4597
Fragments arranged by size:
4597 983 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6394
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2472 2507 3319 3354 529
Size: 35 1908 35 812 35 3569
Fragments arranged by size:
3569 1908 812 35 35 35
DraI TTT'AAA
Cuts at: 3698 3698
Size: 6394
DraIII CAC_nnn'GTG
Cuts at: 2299 2428 2299
Size: 129 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5401 809
Size: 507 4085 1802
Fragments arranged by size:
4085 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2890 3340 3490
Size: 54 1425 50 275 18 1060 450 150
Cuts at: 3490 3841 4730 6359 8
Size: 351 889 1629 43
Fragments arranged by size:
1629 1425 1060 889 450 351 275 150
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3841 1537
Size: 275 2029 4090
Fragments arranged by size:
4090 2029 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 2747 3074 3585 4324 1353
Size: 780 614 327 511 739 3423
Fragments arranged by size:
3423 780 739 614 511 327
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3116 4981 5355 5501 387
Size: 1064 138 1527 1865 374 146 1280
Fragments arranged by size:
1865 1527 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3034 3297 3034
Size: 263 6131
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2717 3130 3223 3423 3695 4457 5054
Size: 393 1085 413 93 200 272 762 597
Cuts at: 5054 5841 6109 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1085 787 762 597 413 393 272
268 200 93
EcoNI CCTnn'n_nnAGG
Cuts at: 3639 3639
Size: 6394
EcoO109I rG'GnC_Cy
Cuts at: 2435 2460 2435
Size: 25 6369
EcoRI G'AATT_C
Cuts at: 3703 4081 3703
Size: 378 6016
FspI TGC'GCA
Cuts at: 4900 4900
Size: 6394
HaeII r_GCGC'y
Cuts at: 1678 3009 5541 1678
Size: 1331 2532 2531
Fragments arranged by size:
2532 2531 1331
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2204 2236 2542 2574 2639
Size: 32 135 32 632 32 306 32 65
Cuts at: 2639 2671 2978 3010 5035 5067 5109 5141 1373
Size: 32 307 32 2025 32 42 32 2626
Fragments arranged by size:
2626 2025 632 307 306 135 65 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3025 3217 3738 3984 669
Size: 988 281 1087 192 521 246 3079
Fragments arranged by size:
3079 1087 988 521 281 246 192
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6394
HpaI GTT'AAC
Cuts at: 3984 3984
Size: 6394
KpnI G_GTAC'C
Cuts at: 3719 3719
Size: 6394
MfeI C'AATT_G
Cuts at: 3993 3993
Size: 6394
MluI A'CGCG_T
Cuts at: 6270 6270
Size: 6394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2190 2407 2767 5508 5692 1305
Size: 885 217 360 2741 184 2007
Fragments arranged by size:
2741 2007 885 360 217 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4572
Fragments arranged by size:
4572 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2424 3164 4352 4711 4870 518
Size: 648 1258 740 1188 359 159 2042
Fragments arranged by size:
2042 1258 1188 740 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2086 3019 3810 4487 5635 5880 1606
Size: 480 933 791 677 1148 245 2120
Fragments arranged by size:
2120 1148 933 791 677 480 245
NaeI GCC'GGC
Cuts at: 2894 2894
Size: 6394
NcoI C'CATG_G
Cuts at: 513 1065 2004 3410 513
Size: 552 939 1406 3497
Fragments arranged by size:
3497 1406 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6394
NgoMIV G'CCGG_C
Cuts at: 2892 2892
Size: 6394
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6394
NotI GC'GGCC_GC
Cuts at: 3841 3841
Size: 6394
NspI r_CATG'y
Cuts at: 1132 1229 2963 3752 5297 1132
Size: 97 1734 789 1545 2229
Fragments arranged by size:
2229 1734 1545 789 97
PasI CC'CwG_GG
Cuts at: 1348 2116 2405 1348
Size: 768 289 5337
Fragments arranged by size:
5337 768 289
PciI A'CATG_T
Cuts at: 2959 5293 2959
Size: 2334 4060
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2695 3411 3446 1108
Size: 1587 716 35 4056
Fragments arranged by size:
4056 1587 716 35
PfoI T'CCnGG_A
Cuts at: 2694 3183 2694
Size: 489 5905
PmeI GTTT'AAAC
Cuts at: 3698 3698
Size: 6394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3180 3212 4463 4495 5976 6008 3180
Size: 32 1251 32 1481 32 3566
Fragments arranged by size:
3566 1481 1251 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2435 2460 2435
Size: 25 6369
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6394
PsiI TTA'TAA
Cuts at: 3964 3964
Size: 6394
PstI C_TGCA'G
Cuts at: 830 2922 3746 830
Size: 2092 824 3478
Fragments arranged by size:
3478 2092 824
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6394
SacI G_AGCT'C
Cuts at: 3036 3299 3036
Size: 263 6131
SalI G'TCGA_C
Cuts at: 1936 3736 1936
Size: 1800 4594
SapI GCTCTTCn'nnn_
Cuts at: 2133 3074 2133
Size: 941 5453
SbfI CC_TGCA'GG
Cuts at: 3746 3746
Size: 6394
ScaI AGT'ACT
Cuts at: 4642 6354 4642
Size: 1712 4682
SfcI C'TryA_G
Cuts at: 826 1045 2583 2878 2918 3742 4877 5558 5749
Size: 219 1538 295 40 824 1135 681 191
Cuts at: 5749 826
Size: 1471
Fragments arranged by size:
1538 1471 1135 824 681 295 219 191
40
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6394
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6394
SmaI CCC'GGG
Cuts at: 1872 3721 1872
Size: 1849 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4498 5399 5661 820
Size: 197 926 2555 901 262 1553
Fragments arranged by size:
2555 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4185 493
Size: 3692 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6394
SphI G_CATG'C
Cuts at: 1132 3752 1132
Size: 2620 3774
SspI AAT'ATT
Cuts at: 5 52 4318 5
Size: 47 4266 2081
Fragments arranged by size:
4266 2081 47
StuI AGG'CCT
Cuts at: 1517 2489 2589 1517
Size: 972 100 5322
Fragments arranged by size:
5322 972 100
StyI C'CwwG_G
Cuts at: 513 1065 2004 2026 2490 3410 3518 3833 513
Size: 552 939 22 464 920 108 315 3074
Fragments arranged by size:
3074 939 920 552 464 315 108 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2098 3373 3403 3670 4421 4438 4591
Size: 1255 518 1275 30 267 751 17 153
Cuts at: 4591 4776 5221 325
Size: 185 445 1498
Fragments arranged by size:
1498 1275 1255 751 518 445 267 185
153 30 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4640 6352 96
Size: 274 80 33 51 1443 2663 1712 138
Fragments arranged by size:
2663 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2274 2306 3472 3504 3645 3677 6354 6386 2274
Size: 32 1166 32 141 32 2677 32 2282
Fragments arranged by size:
2677 2282 1166 141 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6394
XbaI T'CTAG_A
Cuts at: 2827 3730 2827
Size: 903 5491
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6394
XmaI C'CCGG_G
Cuts at: 1870 3719 1870
Size: 1849 4545
XmnI GAAnn'nnTTC
Cuts at: 4523 4523
Size: 6394
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4202 276
Size: 53 83 186 936 2668 2468
Fragments arranged by size:
2668 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI