Restriction map for
pFN21AE4520
(Circular) (Six-base) MAPSORT of: pFN21AE4520.seq Check: 4639 from: 1 to: 6376
pFN21AE4520 6376 bp
With 182 enzymes: SgfI *
March 28, 2012 14:08 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4186 278
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3719 1937
Size: 1782 4594
Acc65I G'GTAC_C
Cuts at: 3697 3697
Size: 6376
AclI AA'CG_TT
Cuts at: 1563 4503 4876 1563
Size: 2940 373 3063
Fragments arranged by size:
3063 2940 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4439 5823 6091 1632
Size: 2807 1384 268 1917
Fragments arranged by size:
2807 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 3667 820
Size: 197 2650 3529
Fragments arranged by size:
3529 2650 197
AflIII A'CryG_T
Cuts at: 1203 3126 3333 3583 3652 5275 6252 1203
Size: 1923 207 250 69 1623 977 1327
Fragments arranged by size:
1923 1623 1327 977 250 207 69
AgeI A'CCGG_T
Cuts at: 5207 5207
Size: 6376
AhdI GACnn_n'nnGTC
Cuts at: 5105 5105
Size: 6376
AleI CACnn'nnGTG
Cuts at: 1166 3146 3584 1166
Size: 1980 438 3958
Fragments arranged by size:
3958 1980 438
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6376
AlwNI CAG_nnn'CTG
Cuts at: 4119 5691 4119
Size: 1572 4804
ApaLI G'TGCA_C
Cuts at: 4433 5589 4433
Size: 1156 5220
ApoI r'AATT_y
Cuts at: 1493 2678 2954 2979 3533 3542 3685 3911 4063
Size: 1185 276 25 554 9 143 226 152
Cuts at: 4063 6172 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1185 554 276 226 152 143
25 9
AseI AT'TA_AT
Cuts at: 160 1032 2426 3594 4930 160
Size: 872 1394 1168 1336 1606
Fragments arranged by size:
1606 1394 1336 1168 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
AvaI C'yCGr_G
Cuts at: 1870 1943 3701 1870
Size: 73 1758 4545
Fragments arranged by size:
4545 1758 73
BamHI G'GATC_C
Cuts at: 3428 3706 4073 3428
Size: 278 367 5731
Fragments arranged by size:
5731 367 278
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3565 3697 5152 618
Size: 325 217 627 1778 132 1455 1842
Fragments arranged by size:
1842 1778 1455 627 325 217 132
BanII G_rGCy'C
Cuts at: 1391 3695 1391
Size: 2304 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2336 2598 928
Size: 971 437 262 4706
Fragments arranged by size:
4706 971 437 262
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4567 4601 1903
Size: 34 2630 34 3678
Fragments arranged by size:
3678 2630 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4268 5478 4268
Size: 1210 5166
BclI T'GATC_A
Cuts at: 1548 2319 1548
Size: 771 5605
BfrBI ATG'CAT
Cuts at: 2784 2784
Size: 6376
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4987 136
Size: 107 122 71 631 605 3315 1525
Fragments arranged by size:
3315 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2231 6371 2231
Size: 4140 2236
BlpI GC'TnA_GC
Cuts at: 2763 3793 2763
Size: 1030 5346
Bme1580I G_kGCm'C
Cuts at: 1790 4437 5593 1790
Size: 2647 1156 2573
Fragments arranged by size:
2647 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3334 1318
Size: 2016 4360
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3523 4107 5065
Size: 416 53 83 399 24 2106 584 958
Cuts at: 5065 442
Size: 1753
Fragments arranged by size:
2106 1753 958 584 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6376
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2441 5036 1239
Size: 1202 2595 2579
Fragments arranged by size:
2595 2579 1202
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2455 2676 3486 4501 5366 5664 2455
Size: 221 810 1015 865 298 3167
Fragments arranged by size:
3167 1015 865 810 298 221
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5039 882
Size: 617 3540 2219
Fragments arranged by size:
3540 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4167 493
Size: 711 2963 2702
Fragments arranged by size:
2963 2702 711
BsaBI GATnn'nnATC
Cuts at: 6370 6370
Size: 6376
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4183 4565 5200 275
Size: 53 83 186 936 2650 382 635 1451
Fragments arranged by size:
2650 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2820 4809 5207 5481 5628 6039 2820
Size: 1989 398 274 147 411 3157
Fragments arranged by size:
3157 1989 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2185 2215 3284 3314 1178
Size: 30 165 30 782 30 1069 30 4240
Fragments arranged by size:
4240 1069 782 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2638 3382 1186
Size: 1452 744 4180
Fragments arranged by size:
4180 1452 744
BseYI C'CCAG_C
Cuts at: 2904 3219 5579 6075 2904
Size: 315 2360 496 3205
Fragments arranged by size:
3205 2360 496 315
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5803
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3826 4587 5615 1540
Size: 275 186 1825 761 1028 2301
Fragments arranged by size:
2301 1825 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4437 4522 5593 4437
Size: 85 1071 5220
Fragments arranged by size:
5220 1071 85
BsmI GAATG_Cn'
Cuts at: 2043 2179 3887 3980 2043
Size: 136 1708 93 4439
Fragments arranged by size:
4439 1708 136 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3695 4437 4522 5593 1391
Size: 399 1905 742 85 1071 2174
Fragments arranged by size:
2174 1905 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2820 6039 2820
Size: 3219 3157
BspHI T'CATG_A
Cuts at: 3375 4265 5995 3375
Size: 890 1730 3756
Fragments arranged by size:
3756 1730 890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2706 3731 844
Size: 1862 1025 3489
Fragments arranged by size:
3489 1862 1025
BsrBI CCG'CTC
Cuts at: 1394 1823 4263 1394
Size: 429 2440 3507
Fragments arranged by size:
3507 2440 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4871 5045 65
Size: 1183 3623 174 1396
Fragments arranged by size:
3623 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5020 5207 1700
Size: 110 3210 187 2869
Fragments arranged by size:
3210 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3336 96
Size: 3240 3136
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6376
BssSI C'ACGA_G
Cuts at: 4436 5448 4436
Size: 1012 5364
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6376
BstBI TT'CG_AA
Cuts at: 5180 5180
Size: 6376
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6376
BstXI CCAn_nnnn'nTGG
Cuts at: 2912 2912
Size: 6376
BstYI r'GATC_y
Cuts at: 1972 2180 2231 3428 3706 4073 4458 4475 5927
Size: 208 51 1197 278 367 385 17 1452
Cuts at: 5927 6013 6025 6371 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1197 385 367 346 278 208
86 51 17 12
Bsu36I CC'TnA_GG
Cuts at: 2513 2513
Size: 6376
BtgI C'CryG_G
Cuts at: 513 1065 2004 3432 513
Size: 552 939 1428 3457
Fragments arranged by size:
3457 1428 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2262 2743 3405 487
Size: 683 56 1036 481 662 3458
Fragments arranged by size:
3458 1036 683 662 481 56
BtsI GCAGTG_nn'
Cuts at: 3312 3892 4686 4706 3312
Size: 580 794 20 4982
Fragments arranged by size:
4982 794 580 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6376
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6341
DraI TTT'AAA
Cuts at: 3680 3680
Size: 6376
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5383 809
Size: 507 4067 1802
Fragments arranged by size:
4067 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2569 3823 4712
Size: 54 1425 50 275 18 739 1254 889
Cuts at: 4712 6341 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1254 889 739 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3823 1537
Size: 275 2011 4090
Fragments arranged by size:
4090 2011 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3453 4306 1353
Size: 2100 853 3423
Fragments arranged by size:
3423 2100 853
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4963 5337 5483 387
Size: 1064 138 3374 374 146 1280
Fragments arranged by size:
3374 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2441 4439 5036 5823 6091 1239
Size: 393 809 1998 597 787 268 1524
Fragments arranged by size:
1998 1524 809 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2609 2609
Size: 6376
EcoRI G'AATT_C
Cuts at: 3685 4063 3685
Size: 378 5998
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2079 2421 2079
Size: 342 6034
FspI TGC'GCA
Cuts at: 4882 4882
Size: 6376
HaeII r_GCGC'y
Cuts at: 1678 5523 1678
Size: 3845 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2198 2230 5017 5049 5091
Size: 32 135 32 626 32 2787 32 42
Cuts at: 5091 5123 1373
Size: 32 2626
Fragments arranged by size:
2787 2626 626 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3664 3720 3966 669
Size: 988 281 1726 56 246 3079
Fragments arranged by size:
3079 1726 988 281 246 56
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6376
HpaI GTT'AAC
Cuts at: 3966 3966
Size: 6376
KpnI G_GTAC'C
Cuts at: 3701 3701
Size: 6376
MfeI C'AATT_G
Cuts at: 3975 3975
Size: 6376
MluI A'CGCG_T
Cuts at: 3126 6252 3126
Size: 3126 3250
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5490 5674 1305
Size: 4185 184 2007
Fragments arranged by size:
4185 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2571 10
Size: 54 1425 343 739 3815
Fragments arranged by size:
3815 1425 739 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2577 3146 3584 4334 4693 4852 518
Size: 648 1411 569 438 750 359 159 2042
Fragments arranged by size:
2042 1411 750 648 569 438 359 159
MspA1I CmG'CkG
Cuts at: 1606 2908 3792 4469 5617 5862 1606
Size: 1302 884 677 1148 245 2120
Fragments arranged by size:
2120 1302 1148 884 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3432 513
Size: 552 939 1428 3457
Fragments arranged by size:
3457 1428 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6376
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6376
NotI GC'GGCC_GC
Cuts at: 3823 3823
Size: 6376
NruI TCG'CGA
Cuts at: 3424 3424
Size: 6376
NsiI A_TGCA'T
Cuts at: 2786 2786
Size: 6376
NspI r_CATG'y
Cuts at: 1132 1229 3587 3656 3734 5279 1132
Size: 97 2358 69 78 1545 2229
Fragments arranged by size:
2358 2229 1545 97 78 69
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6376
PciI A'CATG_T
Cuts at: 3583 3652 5275 3583
Size: 69 1623 4684
Fragments arranged by size:
4684 1623 69
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6376
PfoI T'CCnGG_A
Cuts at: 2967 3399 2967
Size: 432 5944
PmeI GTTT'AAAC
Cuts at: 3680 3680
Size: 6376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4445 4477 5958 5990 4445
Size: 32 1481 32 4831
Fragments arranged by size:
4831 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2419 1613
Size: 806 5570
PsiI TTA'TAA
Cuts at: 3946 3946
Size: 6376
PstI C_TGCA'G
Cuts at: 830 2286 3728 830
Size: 1456 1442 3478
Fragments arranged by size:
3478 1456 1442
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6376
PvuII CAG'CTG
Cuts at: 2908 2908
Size: 6376
SalI G'TCGA_C
Cuts at: 1936 3718 1936
Size: 1782 4594
SbfI CC_TGCA'GG
Cuts at: 3728 3728
Size: 6376
ScaI AGT'ACT
Cuts at: 4624 6336 4624
Size: 1712 4664
SfcI C'TryA_G
Cuts at: 826 1045 2282 2853 2868 3724 4859 5540 5731
Size: 219 1237 571 15 856 1135 681 191
Cuts at: 5731 826
Size: 1471
Fragments arranged by size:
1471 1237 1135 856 681 571 219 191
15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6376
SmaI CCC'GGG
Cuts at: 1872 3703 1872
Size: 1831 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2434 2691 3501 3667 4480 5381
Size: 197 926 491 257 810 166 813 901
Cuts at: 5381 5643 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 813 810 491 262 257
197 166
SnaBI TAC'GTA
Cuts at: 493 4167 493
Size: 3674 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6376
SphI G_CATG'C
Cuts at: 1132 3734 1132
Size: 2602 3774
SspI AAT'ATT
Cuts at: 5 52 4300 5
Size: 47 4248 2081
Fragments arranged by size:
4248 2081 47
StuI AGG'CCT
Cuts at: 1517 2487 1517
Size: 970 5406
StyI C'CwwG_G
Cuts at: 513 1065 2004 3432 3815 513
Size: 552 939 1428 383 3074
Fragments arranged by size:
3074 1428 939 552 383
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4403 4420 4573 4758 5203 325
Size: 1255 2823 17 153 185 445 1498
Fragments arranged by size:
2823 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2141 3336 4622
Size: 274 80 33 51 1443 164 1195 1286
Cuts at: 4622 6334 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1286 1195 274 164 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2185 2217 3420 3452 6336 6368 2185
Size: 32 1203 32 2884 32 2193
Fragments arranged by size:
2884 2193 1203 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6376
XbaI T'CTAG_A
Cuts at: 2353 3157 3712 2353
Size: 804 555 5017
Fragments arranged by size:
5017 804 555
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2912 2912
Size: 6376
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6376
XmaI C'CCGG_G
Cuts at: 1870 3701 1870
Size: 1831 4545
XmnI GAAnn'nnTTC
Cuts at: 2657 3314 4505 2657
Size: 657 1191 4528
Fragments arranged by size:
4528 1191 657
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4184 276
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI