Restriction map for
pFN21AE4515
(Circular) (Six-base) MAPSORT of: pFN21AE4515.seq Check: 9907 from: 1 to: 6346
pFN21AE4515 6346 bp
With 182 enzymes: SgfI *
March 28, 2012 14:07 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4156 278
Size: 53 83 186 936 2620 2468
Fragments arranged by size:
2620 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2109 2435 3689 1937
Size: 172 326 1254 4594
Fragments arranged by size:
4594 1254 326 172
Acc65I G'GTAC_C
Cuts at: 3667 3667
Size: 6346
AclI AA'CG_TT
Cuts at: 1563 4473 4846 1563
Size: 2910 373 3063
Fragments arranged by size:
3063 2910 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2031 2673 2697 2726 3236 4409 5793 6061
Size: 399 642 24 29 510 1173 1384 268
Cuts at: 6061 1632
Size: 1917
Fragments arranged by size:
1917 1384 1173 642 510 399 268 29
24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6149
AflIII A'CryG_T
Cuts at: 1203 5245 6222 1203
Size: 4042 977 1327
Fragments arranged by size:
4042 1327 977
AgeI A'CCGG_T
Cuts at: 5177 5177
Size: 6346
AhdI GACnn_n'nnGTC
Cuts at: 5075 5075
Size: 6346
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6346
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3037 1184
Size: 1853 4493
AlwNI CAG_nnn'CTG
Cuts at: 2704 4089 5661 2704
Size: 1385 1572 3389
Fragments arranged by size:
3389 1572 1385
ApaLI G'TGCA_C
Cuts at: 4403 5559 4403
Size: 1156 5190
ApoI r'AATT_y
Cuts at: 1493 2404 3065 3146 3655 3881 4033 6142 1493
Size: 911 661 81 509 226 152 2109 1697
Fragments arranged by size:
2109 1697 911 661 509 226 152 81
AseI AT'TA_AT
Cuts at: 160 1032 4900 160
Size: 872 3868 1606
Fragments arranged by size:
3868 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6346
AvaI C'yCGr_G
Cuts at: 1870 1943 2840 3671 1870
Size: 73 897 831 4545
Fragments arranged by size:
4545 897 831 73
BamHI G'GATC_C
Cuts at: 2756 3676 4043 2756
Size: 920 367 5059
Fragments arranged by size:
5059 920 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 3354 3627 3667 5122
Size: 325 217 627 335 1232 273 40 1455
Cuts at: 5122 618
Size: 1842
Fragments arranged by size:
1842 1455 1232 627 335 325 273 217
40
BanII G_rGCy'C
Cuts at: 1391 3665 1391
Size: 2274 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2842 3532 928
Size: 971 943 690 3742
Fragments arranged by size:
3742 971 943 690
BbvCI CC'TCA_GC
Cuts at: 3557 3557
Size: 6346
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4537 4571 1903
Size: 34 2600 34 3678
Fragments arranged by size:
3678 2600 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4238 5448 4238
Size: 1210 5136
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6346
BfrBI ATG'CAT
Cuts at: 3248 3248
Size: 6346
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2708 3500 4957
Size: 107 122 71 631 605 1036 792 1457
Cuts at: 4957 136
Size: 1525
Fragments arranged by size:
1525 1457 1036 792 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6341 6341
Size: 6346
BlpI GC'TnA_GC
Cuts at: 3098 3763 3098
Size: 665 5681
Bme1580I G_kGCm'C
Cuts at: 1790 3357 4407 5563 1790
Size: 1567 1050 1156 2573
Fragments arranged by size:
2573 1567 1156 1050
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6346
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2755 4077 5035
Size: 416 53 83 399 24 1338 1322 958
Cuts at: 5035 442
Size: 1753
Fragments arranged by size:
1753 1338 1322 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2822 1056
Size: 1766 4580
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2584 2733 5006 1239
Size: 812 533 149 2273 2579
Fragments arranged by size:
2579 2273 812 533 149
Bpu10I CC'TnA_GC
Cuts at: 3557 3557
Size: 6346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2024 2167 4471 5336 5634 2024
Size: 143 2304 865 298 2736
Fragments arranged by size:
2736 2304 865 298 143
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5009 882
Size: 617 3510 2219
Fragments arranged by size:
3510 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2194 3309 4137 493
Size: 711 990 1115 828 2702
Fragments arranged by size:
2702 1115 990 828 711
BsaBI GATnn'nnATC
Cuts at: 6340 6340
Size: 6346
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4153 4535 5170 275
Size: 53 83 186 936 2620 382 635 1451
Fragments arranged by size:
2620 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4779 5177 5451 5598 6009 4779
Size: 398 274 147 411 5116
Fragments arranged by size:
5116 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6121
Fragments arranged by size:
6121 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3237 1186
Size: 2051 4295
BseYI C'CCAG_C
Cuts at: 5549 6045 5549
Size: 496 5850
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5773
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3796 4557 5585 1540
Size: 275 186 1795 761 1028 2301
Fragments arranged by size:
2301 1795 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2153 3104 4407 4492 5563 2153
Size: 951 1303 85 1071 2936
Fragments arranged by size:
2936 1303 1071 951 85
BsmI GAATG_Cn'
Cuts at: 2384 3121 3248 3857 3950 2384
Size: 737 127 609 93 4780
Fragments arranged by size:
4780 737 609 127 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2153 3104 3357 3665 4407 4492 5563
Size: 399 363 951 253 308 742 85 1071
Cuts at: 5563 1391
Size: 2174
Fragments arranged by size:
2174 1071 951 742 399 363 308 253
85
BspEI T'CCGG_A
Cuts at: 6009 6009
Size: 6346
BspHI T'CATG_A
Cuts at: 2602 4235 5965 2602
Size: 1633 1730 2983
Fragments arranged by size:
2983 1730 1633
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3701 844
Size: 2857 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4233 1394
Size: 429 2410 3507
Fragments arranged by size:
3507 2410 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2670 2829 4841 5015 65
Size: 1183 1422 159 2012 174 1396
Fragments arranged by size:
2012 1422 1396 1183 174 159
BsrFI r'CCGG_y
Cuts at: 1700 1810 4990 5177 1700
Size: 110 3180 187 2869
Fragments arranged by size:
3180 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2802 3380 96
Size: 2706 578 3062
Fragments arranged by size:
3062 2706 578
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6346
BssSI C'ACGA_G
Cuts at: 4406 5418 4406
Size: 1012 5334
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2828 794
Size: 2034 4312
BstBI TT'CG_AA
Cuts at: 5150 5150
Size: 6346
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6346
BstXI CCAn_nnnn'nTGG
Cuts at: 2797 2797
Size: 6346
BstYI r'GATC_y
Cuts at: 1972 2756 3676 4043 4428 4445 5897 5983 5995
Size: 784 920 367 385 17 1452 86 12
Cuts at: 5995 6341 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 920 784 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2436 2436
Size: 6346
Bsu36I CC'TnA_GG
Cuts at: 3075 3075
Size: 6346
BtgI C'CryG_G
Cuts at: 513 1065 2004 2019 513
Size: 552 939 15 4840
Fragments arranged by size:
4840 939 552 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5607
Fragments arranged by size:
5607 683 56
BtsI GCAGTG_nn'
Cuts at: 2865 3862 4656 4676 2865
Size: 997 794 20 4535
Fragments arranged by size:
4535 997 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6346
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6311
DraI TTT'AAA
Cuts at: 3650 3650
Size: 6346
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2792 5353 809
Size: 507 1476 2561 1802
Fragments arranged by size:
2561 1802 1476 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 3793 4682
Size: 54 1425 50 275 18 186 1777 889
Cuts at: 4682 6311 8
Size: 1629 43
Fragments arranged by size:
1777 1629 1425 889 275 186 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3793 1537
Size: 275 1981 4090
Fragments arranged by size:
4090 1981 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2344 2649 3212 3266 4276 1353
Size: 991 305 563 54 1010 3423
Fragments arranged by size:
3423 1010 991 563 305 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4933 5307 5453 387
Size: 1064 138 3344 374 146 1280
Fragments arranged by size:
3344 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 2051 2584 2673 2697 2726 2733
Size: 393 399 20 533 89 24 29 7
Cuts at: 2733 3236 4409 5006 5793 6061 1239
Size: 503 1173 597 787 268 1524
Fragments arranged by size:
1524 1173 787 597 533 503 399 393
268 89 29 24 20 7
EcoO109I rG'GnC_Cy
Cuts at: 2701 2701
Size: 6346
EcoRI G'AATT_C
Cuts at: 3146 3655 4033 3146
Size: 509 378 5459
Fragments arranged by size:
5459 509 378
FspI TGC'GCA
Cuts at: 4852 4852
Size: 6346
HaeII r_GCGC'y
Cuts at: 1678 5493 1678
Size: 3815 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2321 2353 3053 3068 3085
Size: 32 135 32 749 32 700 15 17
Cuts at: 3085 3100 3322 3354 4987 5019 5061 5093 1373
Size: 15 222 32 1633 32 42 32 2626
Fragments arranged by size:
2626 1633 749 700 222 135 42 32
32 32 32 32 32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2329 3690 3936 669
Size: 988 281 391 1361 246 3079
Fragments arranged by size:
3079 1361 988 391 281 246
HindIII A'AGCT_T
Cuts at: 748 2998 3187 3549 748
Size: 2250 189 362 3545
Fragments arranged by size:
3545 2250 362 189
HpaI GTT'AAC
Cuts at: 3936 3936
Size: 6346
KpnI G_GTAC'C
Cuts at: 3671 3671
Size: 6346
MfeI C'AATT_G
Cuts at: 3945 3945
Size: 6346
MluI A'CGCG_T
Cuts at: 6222 6222
Size: 6346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2367 5460 5644 1305
Size: 1062 3093 184 2007
Fragments arranged by size:
3093 2007 1062 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4524
Fragments arranged by size:
4524 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3494 4304 4663 4822 518
Size: 648 2328 810 359 159 2042
Fragments arranged by size:
2328 2042 810 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3032 3762 4439 5587 5832 1606
Size: 1426 730 677 1148 245 2120
Fragments arranged by size:
2120 1426 1148 730 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4855
Fragments arranged by size:
4855 939 552
NdeI CA'TA_TG
Cuts at: 387 2956 3244 387
Size: 2569 288 3489
Fragments arranged by size:
3489 2569 288
NheI G'CTAG_C
Cuts at: 1052 2818 1052
Size: 1766 4580
NotI GC'GGCC_GC
Cuts at: 3793 3793
Size: 6346
NsiI A_TGCA'T
Cuts at: 3250 3250
Size: 6346
NspI r_CATG'y
Cuts at: 1132 1229 2164 3704 5249 1132
Size: 97 935 1540 1545 2229
Fragments arranged by size:
2229 1545 1540 935 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6346
PciI A'CATG_T
Cuts at: 5245 5245
Size: 6346
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3624 1108
Size: 2516 3830
PfoI T'CCnGG_A
Cuts at: 3522 3522
Size: 6346
PmeI GTTT'AAAC
Cuts at: 3650 3650
Size: 6346
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4415 4447 5928 5960 4415
Size: 32 1481 32 4801
Fragments arranged by size:
4801 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6346
PsiI TTA'TAA
Cuts at: 3916 3916
Size: 6346
PstI C_TGCA'G
Cuts at: 830 2599 3698 830
Size: 1769 1099 3478
Fragments arranged by size:
3478 1769 1099
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6346
PvuII CAG'CTG
Cuts at: 3032 3032
Size: 6346
SalI G'TCGA_C
Cuts at: 1936 3688 1936
Size: 1752 4594
SbfI CC_TGCA'GG
Cuts at: 3698 3698
Size: 6346
ScaI AGT'ACT
Cuts at: 4594 6306 4594
Size: 1712 4634
SfcI C'TryA_G
Cuts at: 826 1045 2595 3694 4829 5510 5701 826
Size: 219 1550 1099 1135 681 191 1471
Fragments arranged by size:
1550 1471 1135 1099 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6346
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6346
SmaI CCC'GGG
Cuts at: 1872 3673 1872
Size: 1801 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2039 2146 4450 5351 5613 820
Size: 197 926 96 107 2304 901 262 1553
Fragments arranged by size:
2304 1553 926 901 262 197 107 96
SnaBI TAC'GTA
Cuts at: 493 4137 493
Size: 3644 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6346
SphI G_CATG'C
Cuts at: 1132 3704 1132
Size: 2572 3774
SspI AAT'ATT
Cuts at: 5 52 4270 5
Size: 47 4218 2081
Fragments arranged by size:
4218 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6346
StyI C'CwwG_G
Cuts at: 513 1065 2004 2610 3342 3785 513
Size: 552 939 606 732 443 3074
Fragments arranged by size:
3074 939 732 606 552 443
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2431 3411 4373 4390 4543 4728 5173
Size: 1255 851 980 962 17 153 185 445
Cuts at: 5173 325
Size: 1498
Fragments arranged by size:
1498 1255 980 962 851 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2802 3380 4592
Size: 274 80 33 51 1443 825 578 1212
Cuts at: 4592 6304 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1212 825 578 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3504 3536 6306 6338 3504
Size: 32 2770 32 3512
Fragments arranged by size:
3512 2770 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6346
XbaI T'CTAG_A
Cuts at: 3682 3682
Size: 6346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2026 3621 2026
Size: 1595 4751
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6346
XmaI C'CCGG_G
Cuts at: 1870 3671 1870
Size: 1801 4545
XmnI GAAnn'nnTTC
Cuts at: 2888 2937 4475 2888
Size: 49 1538 4759
Fragments arranged by size:
4759 1538 49
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4154 276
Size: 53 83 186 936 2620 2468
Fragments arranged by size:
2620 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI