Restriction map for
pFN21AE4514
(Circular) (Six-base) MAPSORT of: pFN21AE4514.seq Check: 3775 from: 1 to: 6343
pFN21AE4514 6343 bp
With 182 enzymes: SgfI *
March 28, 2012 14:07 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4153 278
Size: 53 83 186 936 2617 2468
Fragments arranged by size:
2617 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2687 3686 1937
Size: 750 999 4594
Fragments arranged by size:
4594 999 750
Acc65I G'GTAC_C
Cuts at: 3664 3664
Size: 6343
AclI AA'CG_TT
Cuts at: 1563 3085 4470 4843 1563
Size: 1522 1385 373 3063
Fragments arranged by size:
3063 1522 1385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2588 2910 4406 5790 6058 1632
Size: 956 322 1496 1384 268 1917
Fragments arranged by size:
1917 1496 1384 956 322 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6146
AflIII A'CryG_T
Cuts at: 1203 5242 6219 1203
Size: 4039 977 1327
Fragments arranged by size:
4039 1327 977
AgeI A'CCGG_T
Cuts at: 5174 5174
Size: 6343
AhdI GACnn_n'nnGTC
Cuts at: 5072 5072
Size: 6343
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6343
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3047 1184
Size: 1863 4480
AlwNI CAG_nnn'CTG
Cuts at: 2460 4086 5658 2460
Size: 1626 1572 3145
Fragments arranged by size:
3145 1626 1572
ApaI G_GGCC'C
Cuts at: 2347 2764 3409 3508 2347
Size: 417 645 99 5182
Fragments arranged by size:
5182 645 417 99
ApaLI G'TGCA_C
Cuts at: 4400 5556 4400
Size: 1156 5187
ApoI r'AATT_y
Cuts at: 1493 2429 3652 3878 4030 6139 1493
Size: 936 1223 226 152 2109 1697
Fragments arranged by size:
2109 1697 1223 936 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4897 160
Size: 872 3865 1606
Fragments arranged by size:
3865 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6343
AvaI C'yCGr_G
Cuts at: 1870 1943 2040 3668 1870
Size: 73 97 1628 4545
Fragments arranged by size:
4545 1628 97 73
BamHI G'GATC_C
Cuts at: 2161 3673 4040 2161
Size: 1512 367 4464
Fragments arranged by size:
4464 1512 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2481 3664 5119 618
Size: 325 217 627 694 1183 1455 1842
Fragments arranged by size:
1842 1455 1183 694 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2105 2347 2764 3017 3327 3364 3409 3508
Size: 714 242 417 253 310 37 45 99
Cuts at: 3508 3662 1391
Size: 154 4072
Fragments arranged by size:
4072 714 417 310 253 242 154 99
45 37
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2333 928
Size: 971 434 4938
Fragments arranged by size:
4938 971 434
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4534 4568 1903
Size: 34 2597 34 3678
Fragments arranged by size:
3678 2597 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4235 5445 4235
Size: 1210 5133
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6343
BfrBI ATG'CAT
Cuts at: 2244 2244
Size: 6343
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3021 4954 136
Size: 107 122 71 631 605 1349 1933 1525
Fragments arranged by size:
1933 1525 1349 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2416 3142 6338 2416
Size: 726 3196 2421
Fragments arranged by size:
3196 2421 726
BlpI GC'TnA_GC
Cuts at: 3760 3760
Size: 6343
Bme1580I G_kGCm'C
Cuts at: 1790 2347 2713 2764 3409 3508 4404 5560 1790
Size: 557 366 51 645 99 896 1156 2573
Fragments arranged by size:
2573 1156 896 645 557 366 99 51
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6343
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2760 3216 3466
Size: 416 53 83 399 24 1343 456 250
Cuts at: 3466 3485 4074 5032 442
Size: 19 589 958 1753
Fragments arranged by size:
1753 1343 958 589 456 416 399 250
83 53 24 19
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6343
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3220 3220
Size: 6343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3031 5003 1239
Size: 1792 1972 2579
Fragments arranged by size:
2579 1972 1792
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4468 5333 5631 4468
Size: 865 298 5180
Fragments arranged by size:
5180 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2828 5006 882
Size: 617 1329 2178 2219
Fragments arranged by size:
2219 2178 1329 617
BsaAI yAC'GTr
Cuts at: 493 1204 4134 493
Size: 711 2930 2702
Fragments arranged by size:
2930 2702 711
BsaBI GATnn'nnATC
Cuts at: 6337 6337
Size: 6343
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2594 4150 4532 5167
Size: 53 83 186 936 1061 1556 382 635
Cuts at: 5167 275
Size: 1451
Fragments arranged by size:
1556 1451 1061 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4776 5174 5448 5595 6006 4776
Size: 398 274 147 411 5113
Fragments arranged by size:
5113 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2216 2246 2663 2693 2856
Size: 30 165 30 813 30 417 30 163
Cuts at: 2856 2886 1178
Size: 30 4635
Fragments arranged by size:
4635 813 417 165 163 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2019 2877 3194 1186
Size: 833 858 317 4335
Fragments arranged by size:
4335 858 833 317
BseYI C'CCAG_C
Cuts at: 2340 3363 3369 3521 5546 6042 2340
Size: 1023 6 152 2025 496 2641
Fragments arranged by size:
2641 2025 1023 496 152 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5770
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3793 4554 5582 1540
Size: 275 186 1792 761 1028 2301
Fragments arranged by size:
2301 1792 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2067 3327 4404 4489 5560 2012
Size: 55 1260 1077 85 1071 2795
Fragments arranged by size:
2795 1260 1077 1071 85 55
BsmI GAATG_Cn'
Cuts at: 2652 3854 3947 2652
Size: 1202 93 5048
Fragments arranged by size:
5048 1202 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2601 2601
Size: 6343
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2067 2105 2347 2713 2764 3017
Size: 399 222 55 38 242 366 51 253
Cuts at: 3017 3327 3364 3409 3508 3662 4404 4489 5560
Size: 310 37 45 99 154 742 85 1071
Cuts at: 5560 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 366 310 253 242
222 154 99 85 55 51 45 38
37
BspEI T'CCGG_A
Cuts at: 6006 6006
Size: 6343
BspHI T'CATG_A
Cuts at: 3541 4232 5962 3541
Size: 691 1730 3922
Fragments arranged by size:
3922 1730 691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2889 3555 3698 844
Size: 2045 666 143 3489
Fragments arranged by size:
3489 2045 666 143
BsrBI CCG'CTC
Cuts at: 1394 1823 4230 1394
Size: 429 2407 3507
Fragments arranged by size:
3507 2407 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4838 5012 65
Size: 1183 3590 174 1396
Fragments arranged by size:
3590 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2574 4987 5174 1700
Size: 110 764 2413 187 2869
Fragments arranged by size:
2869 2413 764 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6343
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6343
BssSI C'ACGA_G
Cuts at: 4403 5415 4403
Size: 1012 5331
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2578 794
Size: 1784 4559
BstBI TT'CG_AA
Cuts at: 5147 5147
Size: 6343
BstEII G'GTnAC_C
Cuts at: 1184 2872 1184
Size: 1688 4655
BstYI r'GATC_y
Cuts at: 1972 2161 2416 3142 3673 4040 4425 4442 5894
Size: 189 255 726 531 367 385 17 1452
Cuts at: 5894 5980 5992 6338 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 726 531 385 367 346 255
189 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2191 2377 2796 3207 513
Size: 552 1126 186 419 411 3649
Fragments arranged by size:
3649 1126 552 419 411 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5604
Fragments arranged by size:
5604 683 56
BtsI GCAGTG_nn'
Cuts at: 2511 3859 4653 4673 2511
Size: 1348 794 20 4181
Fragments arranged by size:
4181 1348 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6343
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3117 3152 529
Size: 35 2553 35 3720
Fragments arranged by size:
3720 2553 35 35
DraI TTT'AAA
Cuts at: 3647 3647
Size: 6343
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5350 809
Size: 507 4034 1802
Fragments arranged by size:
4034 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3790 4679 6308
Size: 54 1425 50 275 18 1960 889 1629
Cuts at: 6308 8
Size: 43
Fragments arranged by size:
1960 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3790 1537
Size: 275 1978 4090
Fragments arranged by size:
4090 1978 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2231 2301 2374 2704 4273 1353
Size: 878 70 73 330 1569 3423
Fragments arranged by size:
3423 1569 878 330 73 70
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4930 5304 5450 387
Size: 1064 138 3341 374 146 1280
Fragments arranged by size:
3341 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3325 3325
Size: 6343
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2588 2910 3031 4406 5003 5790 6058
Size: 393 956 322 121 1375 597 787 268
Cuts at: 6058 1239
Size: 1524
Fragments arranged by size:
1524 1375 956 787 597 393 322 268
121
EcoNI CCTnn'n_nnAGG
Cuts at: 3597 3597
Size: 6343
EcoO109I rG'GnC_Cy
Cuts at: 2344 2382 2761 3217 3405 3406 3589 2344
Size: 38 379 456 188 1 183 5098
Fragments arranged by size:
5098 456 379 188 183 38 1
EcoRI G'AATT_C
Cuts at: 3652 4030 3652
Size: 378 5965
FspI TGC'GCA
Cuts at: 4849 4849
Size: 6343
HaeII r_GCGC'y
Cuts at: 1678 2995 5490 1678
Size: 1317 2495 2531
Fragments arranged by size:
2531 2495 1317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2146 2178 2401 2433 2663
Size: 32 135 32 574 32 223 32 230
Cuts at: 2663 2695 4984 5016 5058 5090 1373
Size: 32 2289 32 42 32 2626
Fragments arranged by size:
2626 2289 574 230 223 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3687 3933 669
Size: 988 281 1749 246 3079
Fragments arranged by size:
3079 1749 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6343
HpaI GTT'AAC
Cuts at: 3933 3933
Size: 6343
KpnI G_GTAC'C
Cuts at: 3668 3668
Size: 6343
MfeI C'AATT_G
Cuts at: 3942 3942
Size: 6343
MluI A'CGCG_T
Cuts at: 6219 6219
Size: 6343
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3091 5457 5641 1305
Size: 1786 2366 184 2007
Fragments arranged by size:
2366 2007 1786 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4521
Fragments arranged by size:
4521 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4301 4660 4819 518
Size: 648 3135 359 159 2042
Fragments arranged by size:
3135 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2457 2930 3454 3759 4436 5584 5829 1606
Size: 851 473 524 305 677 1148 245 2120
Fragments arranged by size:
2120 1148 851 677 524 473 305 245
NcoI C'CATG_G
Cuts at: 513 1065 2191 2377 3207 513
Size: 552 1126 186 830 3649
Fragments arranged by size:
3649 1126 830 552 186
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6343
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6343
NotI GC'GGCC_GC
Cuts at: 3790 3790
Size: 6343
NsiI A_TGCA'T
Cuts at: 2246 2246
Size: 6343
NspI r_CATG'y
Cuts at: 1132 1229 3701 5246 1132
Size: 97 2472 1545 2229
Fragments arranged by size:
2472 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2729 3599 1348
Size: 1381 870 4092
Fragments arranged by size:
4092 1381 870
PciI A'CATG_T
Cuts at: 5242 5242
Size: 6343
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3213 3497 1108
Size: 2105 284 3954
Fragments arranged by size:
3954 2105 284
PmeI GTTT'AAAC
Cuts at: 3647 3647
Size: 6343
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4412 4444 5925 5957 4412
Size: 32 1481 32 4798
Fragments arranged by size:
4798 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2382 3217 3589 2382
Size: 835 372 5136
Fragments arranged by size:
5136 835 372
PshAI GACnn'nnGTC
Cuts at: 1613 2840 1613
Size: 1227 5116
PsiI TTA'TAA
Cuts at: 3913 3913
Size: 6343
PspOMI G'GGCC_C
Cuts at: 2343 2760 3405 3504 2343
Size: 417 645 99 5182
Fragments arranged by size:
5182 645 417 99
PstI C_TGCA'G
Cuts at: 830 2583 3695 830
Size: 1753 1112 3478
Fragments arranged by size:
3478 1753 1112
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6343
PvuII CAG'CTG
Cuts at: 2457 2930 2457
Size: 473 5870
SacI G_AGCT'C
Cuts at: 3327 3327
Size: 6343
SalI G'TCGA_C
Cuts at: 1936 3685 1936
Size: 1749 4594
SanDI GG'GwC_CC
Cuts at: 2382 3217 3589 2382
Size: 835 372 5136
Fragments arranged by size:
5136 835 372
SbfI CC_TGCA'GG
Cuts at: 3695 3695
Size: 6343
ScaI AGT'ACT
Cuts at: 4591 6303 4591
Size: 1712 4631
SfcI C'TryA_G
Cuts at: 826 1045 2579 3691 4826 5507 5698 826
Size: 219 1534 1112 1135 681 191 1471
Fragments arranged by size:
1534 1471 1135 1112 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6343
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6343
SmaI CCC'GGG
Cuts at: 1872 3670 1872
Size: 1798 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4447 5348 5610 820
Size: 197 926 2504 901 262 1553
Fragments arranged by size:
2504 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4134 493
Size: 3641 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6343
SphI G_CATG'C
Cuts at: 1132 3701 1132
Size: 2569 3774
SspI AAT'ATT
Cuts at: 5 52 4267 5
Size: 47 4215 2081
Fragments arranged by size:
4215 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6343
StyI C'CwwG_G
Cuts at: 513 1065 2191 2377 3207 3260 3584 3782 513
Size: 552 1126 186 830 53 324 198 3074
Fragments arranged by size:
3074 1126 830 552 324 198 186 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2311 4370 4387 4540 4725 5170 325
Size: 1255 731 2059 17 153 185 445 1498
Fragments arranged by size:
2059 1498 1255 731 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3456 4589 6301
Size: 274 80 33 51 1443 1479 1133 1712
Cuts at: 6301 96
Size: 138
Fragments arranged by size:
1712 1479 1443 1133 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2216 2248 2856 2888 3370 3402 6303 6335 2216
Size: 32 608 32 482 32 2901 32 2224
Fragments arranged by size:
2901 2224 608 482 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2525 1316
Size: 1209 5134
XbaI T'CTAG_A
Cuts at: 3679 3679
Size: 6343
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6343
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6343
XmaI C'CCGG_G
Cuts at: 1870 3668 1870
Size: 1798 4545
XmnI GAAnn'nnTTC
Cuts at: 4472 4472
Size: 6343
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4151 276
Size: 53 83 186 936 2617 2468
Fragments arranged by size:
2617 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspXI PsrI RsrII SacII SapI SexAI
SfiI SfoI SrfI SwaI