Restriction map for
pFN21AE4505
(Circular) (Six-base) MAPSORT of: pFN21AE4505.seq Check: 2257 from: 1 to: 6292
pFN21AE4505 6292 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2993 2993
Size: 6292
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4102 278
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3635 1937
Size: 1698 4594
Acc65I G'GTAC_C
Cuts at: 3264 3335 3613 3264
Size: 71 278 5943
Fragments arranged by size:
5943 278 71
AclI AA'CG_TT
Cuts at: 1563 3499 4419 4792 1563
Size: 1936 920 373 3063
Fragments arranged by size:
3063 1936 920 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4355 5739 6007 1632
Size: 2723 1384 268 1917
Fragments arranged by size:
2723 1917 1384 268
AfeI AGC'GCT
Cuts at: 2111 2204 2111
Size: 93 6199
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6095
AflIII A'CryG_T
Cuts at: 1203 2601 5191 6168 1203
Size: 1398 2590 977 1327
Fragments arranged by size:
2590 1398 1327 977
AgeI A'CCGG_T
Cuts at: 5123 5123
Size: 6292
AhdI GACnn_n'nnGTC
Cuts at: 5021 5021
Size: 6292
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6292
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 6260
AlwNI CAG_nnn'CTG
Cuts at: 3079 3423 3440 4035 5607 3079
Size: 344 17 595 1572 3764
Fragments arranged by size:
3764 1572 595 344 17
ApaI G_GGCC'C
Cuts at: 3247 3247
Size: 6292
ApaLI G'TGCA_C
Cuts at: 4349 5505 4349
Size: 1156 5136
ApoI r'AATT_y
Cuts at: 1493 3601 3827 3979 6088 1493
Size: 2108 226 152 2109 1697
Fragments arranged by size:
2109 2108 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4846 160
Size: 872 3814 1606
Fragments arranged by size:
3814 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
AvaI C'yCGr_G
Cuts at: 1870 1943 3617 1870
Size: 73 1674 4545
Fragments arranged by size:
4545 1674 73
BamHI G'GATC_C
Cuts at: 3622 3989 3622
Size: 367 5925
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2858 3264 3335 3613 5068
Size: 325 217 627 1071 406 71 278 1455
Cuts at: 5068 618
Size: 1842
Fragments arranged by size:
1842 1455 1071 627 406 325 278 217
71
BanII G_rGCy'C
Cuts at: 1391 2410 2667 2853 3247 3611 1391
Size: 1019 257 186 394 364 4072
Fragments arranged by size:
4072 1019 394 364 257 186
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2929 928
Size: 971 1030 4291
Fragments arranged by size:
4291 1030 971
BbvCI CC'TCA_GC
Cuts at: 2454 3527 2454
Size: 1073 5219
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4483 4517 1903
Size: 34 2546 34 3678
Fragments arranged by size:
3678 2546 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2521 2765 2822 4184 5394 2521
Size: 244 57 1362 1210 3419
Fragments arranged by size:
3419 1362 1210 244 57
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6292
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4903 136
Size: 107 122 71 631 605 3231 1525
Fragments arranged by size:
3231 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6287 6287
Size: 6292
BlpI GC'TnA_GC
Cuts at: 3709 3709
Size: 6292
Bme1580I G_kGCm'C
Cuts at: 1790 2462 3247 4353 5509 1790
Size: 672 785 1106 1156 2573
Fragments arranged by size:
2573 1156 1106 785 672
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6292
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2874 4023 4981
Size: 416 53 83 399 24 1457 1149 958
Cuts at: 4981 442
Size: 1753
Fragments arranged by size:
1753 1457 1149 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 2441 3077 4952 1239
Size: 1055 147 636 1875 2579
Fragments arranged by size:
2579 1875 1055 636 147
Bpu10I CC'TnA_GC
Cuts at: 2454 3342 3527 2454
Size: 888 185 5219
Fragments arranged by size:
5219 888 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4417 5282 5580 4417
Size: 865 298 5129
Fragments arranged by size:
5129 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4955 882
Size: 617 3456 2219
Fragments arranged by size:
3456 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3310 4083 493
Size: 711 2106 773 2702
Fragments arranged by size:
2702 2106 773 711
BsaBI GATnn'nnATC
Cuts at: 2949 6286 2949
Size: 3337 2955
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4099 4481 5116 275
Size: 53 83 186 936 2566 382 635 1451
Fragments arranged by size:
2566 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4725 5123 5397 5544 5955 4725
Size: 398 274 147 411 5062
Fragments arranged by size:
5062 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2958 2988 3378 3408 1178
Size: 30 165 30 1555 30 390 30 4062
Fragments arranged by size:
4062 1555 390 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2375 2979 1186
Size: 1189 604 4499
Fragments arranged by size:
4499 1189 604
BseYI C'CCAG_C
Cuts at: 5495 5991 5495
Size: 496 5796
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5719
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2163 3742 4503 5531 1540
Size: 275 186 162 1579 761 1028 2301
Fragments arranged by size:
2301 1579 1028 761 275 186 162
BsiHKAI G_wGCw'C
Cuts at: 2410 2667 2912 4353 4438 5509 2410
Size: 257 245 1441 85 1071 3193
Fragments arranged by size:
3193 1441 1071 257 245 85
BsmI GAATG_Cn'
Cuts at: 3803 3896 3803
Size: 93 6199
BsmBI CGTCTCn'nnnn_
Cuts at: 3097 3097
Size: 6292
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2410 2462 2667 2853 2912 3247 3611
Size: 399 620 52 205 186 59 335 364
Cuts at: 3611 4353 4438 5509 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 620 399 364 335 205
186 85 59 52
BspEI T'CCGG_A
Cuts at: 5955 5955
Size: 6292
BspHI T'CATG_A
Cuts at: 2667 2904 4181 5911 2667
Size: 237 1277 1730 3048
Fragments arranged by size:
3048 1730 1277 237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2223 2553 2993 3647 844
Size: 1379 330 440 654 3489
Fragments arranged by size:
3489 1379 654 440 330
BsrBI CCG'CTC
Cuts at: 1394 1823 4179 1394
Size: 429 2356 3507
Fragments arranged by size:
3507 2356 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4787 4961 65
Size: 1183 3539 174 1396
Fragments arranged by size:
3539 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3233 4936 5123 1700
Size: 110 1423 1703 187 2869
Fragments arranged by size:
2869 1703 1423 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6292
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6292
BssSI C'ACGA_G
Cuts at: 4352 5364 4352
Size: 1012 5280
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6292
BstBI TT'CG_AA
Cuts at: 5096 5096
Size: 6292
BstEII G'GTnAC_C
Cuts at: 1184 3223 1184
Size: 2039 4253
BstYI r'GATC_y
Cuts at: 1972 3006 3622 3989 4374 4391 5843 5929 5941
Size: 1034 616 367 385 17 1452 86 12
Cuts at: 5941 6287 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1034 616 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5740
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5553
Fragments arranged by size:
5553 683 56
BtsI GCAGTG_nn'
Cuts at: 3808 4602 4622 3808
Size: 794 20 5478
Fragments arranged by size:
5478 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6257
DraI TTT'AAA
Cuts at: 3596 3596
Size: 6292
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5299 809
Size: 507 3983 1802
Fragments arranged by size:
3983 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 2785 3739
Size: 54 1425 50 275 18 330 625 954
Cuts at: 3739 4628 6257 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 954 889 625 330 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2160 3739 1537
Size: 275 348 1579 4090
Fragments arranged by size:
4090 1579 348 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3100 4222 1353
Size: 1747 1122 3423
Fragments arranged by size:
3423 1747 1122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4879 5253 5399 387
Size: 1064 138 3290 374 146 1280
Fragments arranged by size:
3290 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2408 2665 2408
Size: 257 6035
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2441 3077 4355 4952 5739 6007
Size: 393 662 147 636 1278 597 787 268
Cuts at: 6007 1239
Size: 1524
Fragments arranged by size:
1524 1278 787 662 636 597 393 268
147
EcoNI CCTnn'n_nnAGG
Cuts at: 2189 2189
Size: 6292
EcoO109I rG'GnC_Cy
Cuts at: 2679 3244 2679
Size: 565 5727
EcoRI G'AATT_C
Cuts at: 3601 3979 3601
Size: 378 5914
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3049 3253 3049
Size: 204 6088
FspI TGC'GCA
Cuts at: 4798 4798
Size: 6292
HaeII r_GCGC'y
Cuts at: 1678 2113 2206 5439 1678
Size: 435 93 3233 2531
Fragments arranged by size:
3233 2531 435 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2141 2173 2796 2828 3077
Size: 32 135 32 569 32 623 32 249
Cuts at: 3077 3109 4933 4965 5007 5039 1373
Size: 32 1824 32 42 32 2626
Fragments arranged by size:
2626 1824 623 569 249 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3471 3636 3882 669
Size: 988 281 1533 165 246 3079
Fragments arranged by size:
3079 1533 988 281 246 165
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6292
HpaI GTT'AAC
Cuts at: 3882 3882
Size: 6292
KpnI G_GTAC'C
Cuts at: 3268 3339 3617 3268
Size: 71 278 5943
Fragments arranged by size:
5943 278 71
MfeI C'AATT_G
Cuts at: 3891 3891
Size: 6292
MluI A'CGCG_T
Cuts at: 6168 6168
Size: 6292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2127 2505 3021 5406 5590 1305
Size: 822 378 516 2385 184 2007
Fragments arranged by size:
2385 2007 822 516 378 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2787 10
Size: 54 1425 343 955 3515
Fragments arranged by size:
3515 1425 955 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2311 3173 4250 4609 4768 518
Size: 648 1145 862 1077 359 159 2042
Fragments arranged by size:
2042 1145 1077 862 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3055 3516 3708 4385 5533 5778 1606
Size: 1449 461 192 677 1148 245 2120
Fragments arranged by size:
2120 1449 1148 677 461 245 192
NaeI GCC'GGC
Cuts at: 3235 3235
Size: 6292
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5740
NdeI CA'TA_TG
Cuts at: 387 2641 387
Size: 2254 4038
NgoMIV G'CCGG_C
Cuts at: 3233 3233
Size: 6292
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6292
NotI GC'GGCC_GC
Cuts at: 3739 3739
Size: 6292
NspI r_CATG'y
Cuts at: 1132 1229 3650 5195 1132
Size: 97 2421 1545 2229
Fragments arranged by size:
2421 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6292
PciI A'CATG_T
Cuts at: 5191 5191
Size: 6292
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2022 3423 1108
Size: 914 1401 3977
Fragments arranged by size:
3977 1401 914
PfoI T'CCnGG_A
Cuts at: 2562 3009 2562
Size: 447 5845
PmeI GTTT'AAAC
Cuts at: 3596 3596
Size: 6292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4361 4393 5874 5906 4361
Size: 32 1481 32 4747
Fragments arranged by size:
4747 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2679 2679
Size: 6292
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6292
PsiI TTA'TAA
Cuts at: 3862 3862
Size: 6292
PspOMI G'GGCC_C
Cuts at: 3243 3243
Size: 6292
PstI C_TGCA'G
Cuts at: 830 3644 830
Size: 2814 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6292
PvuII CAG'CTG
Cuts at: 3055 3055
Size: 6292
SacI G_AGCT'C
Cuts at: 2410 2667 2410
Size: 257 6035
SalI G'TCGA_C
Cuts at: 1936 3634 1936
Size: 1698 4594
SbfI CC_TGCA'GG
Cuts at: 3644 3644
Size: 6292
ScaI AGT'ACT
Cuts at: 4540 6252 4540
Size: 1712 4580
SexAI A'CCwGG_T
Cuts at: 2806 3408 2806
Size: 602 5690
SfcI C'TryA_G
Cuts at: 826 1045 2298 3640 4775 5456 5647 826
Size: 219 1253 1342 1135 681 191 1471
Fragments arranged by size:
1471 1342 1253 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6292
SmaI CCC'GGG
Cuts at: 1872 3619 1872
Size: 1747 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4396 5297 5559 820
Size: 197 926 2453 901 262 1553
Fragments arranged by size:
2453 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3310 4083 493
Size: 2817 773 2702
Fragments arranged by size:
2817 2702 773
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6292
SphI G_CATG'C
Cuts at: 1132 3650 1132
Size: 2518 3774
SspI AAT'ATT
Cuts at: 5 52 4216 5
Size: 47 4164 2081
Fragments arranged by size:
4164 2081 47
StuI AGG'CCT
Cuts at: 1517 2195 1517
Size: 678 5614
StyI C'CwwG_G
Cuts at: 513 1065 3228 3731 513
Size: 552 2163 503 3074
Fragments arranged by size:
3074 2163 552 503
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2711 4319 4336 4489 4674 5119 325
Size: 1255 1131 1608 17 153 185 445 1498
Fragments arranged by size:
1608 1498 1255 1131 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4538 6250 96
Size: 274 80 33 51 1443 2561 1712 138
Fragments arranged by size:
2561 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2780 2812 3378 3410 6252 6284 2780
Size: 32 566 32 2842 32 2788
Fragments arranged by size:
2842 2788 566 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3089 1316
Size: 1773 4519
XbaI T'CTAG_A
Cuts at: 3628 3628
Size: 6292
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6292
XmaI C'CCGG_G
Cuts at: 1870 3617 1870
Size: 1747 4545
XmnI GAAnn'nnTTC
Cuts at: 2603 3032 3302 4421 2603
Size: 429 270 1119 4474
Fragments arranged by size:
4474 1119 429 270
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4100 276
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI