Restriction map for
pFN21AE4504
(Circular) (Six-base) MAPSORT of: pFN21AE4504.seq Check: 193 from: 1 to: 6289
pFN21AE4504 6289 bp
With 182 enzymes: SgfI *
March 28, 2012 14:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2458 2458
Size: 6289
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4099 278
Size: 53 83 186 936 2563 2468
Fragments arranged by size:
2563 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3632 1937
Size: 1695 4594
Acc65I G'GTAC_C
Cuts at: 3610 3610
Size: 6289
AclI AA'CG_TT
Cuts at: 1563 4416 4789 1563
Size: 2853 373 3063
Fragments arranged by size:
3063 2853 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2046 2105 2117 2664 2759 2951 4352 5736
Size: 414 59 12 547 95 192 1401 1384
Cuts at: 5736 6004 1632
Size: 268 1917
Fragments arranged by size:
1917 1401 1384 547 414 268 192 95
59 12
AfeI AGC'GCT
Cuts at: 3019 3019
Size: 6289
AflII C'TTAA_G
Cuts at: 820 1017 2816 820
Size: 197 1799 4293
Fragments arranged by size:
4293 1799 197
AflIII A'CryG_T
Cuts at: 1203 2149 3402 5188 6165 1203
Size: 946 1253 1786 977 1327
Fragments arranged by size:
1786 1327 1253 977 946
AgeI A'CCGG_T
Cuts at: 5120 5120
Size: 6289
AhdI GACnn_n'nnGTC
Cuts at: 5018 5018
Size: 6289
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6289
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2348 3168 1184
Size: 1164 820 4305
Fragments arranged by size:
4305 1164 820
AlwNI CAG_nnn'CTG
Cuts at: 3386 4032 5604 3386
Size: 646 1572 4071
Fragments arranged by size:
4071 1572 646
ApaLI G'TGCA_C
Cuts at: 4346 5502 4346
Size: 1156 5133
ApoI r'AATT_y
Cuts at: 1493 2115 2935 3073 3492 3598 3824 3976 6085
Size: 622 820 138 419 106 226 152 2109
Cuts at: 6085 1493
Size: 1697
Fragments arranged by size:
2109 1697 820 622 419 226 152 138
106
AseI AT'TA_AT
Cuts at: 160 1032 4843 160
Size: 872 3811 1606
Fragments arranged by size:
3811 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
AvaI C'yCGr_G
Cuts at: 1870 1943 3614 1870
Size: 73 1671 4545
Fragments arranged by size:
4545 1671 73
BamHI G'GATC_C
Cuts at: 2968 3619 3986 2968
Size: 651 367 5271
Fragments arranged by size:
5271 651 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3610 5065 618
Size: 325 217 627 1823 1455 1842
Fragments arranged by size:
1842 1823 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2678 3447 3551 3608 1391
Size: 1287 769 104 57 4072
Fragments arranged by size:
4072 1287 769 104 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3457 928
Size: 971 1558 3760
Fragments arranged by size:
3760 1558 971
BbvCI CC'TCA_GC
Cuts at: 2454 2907 2454
Size: 453 5836
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4480 4514 1903
Size: 34 2543 34 3678
Fragments arranged by size:
3678 2543 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4181 5391 4181
Size: 1210 5079
BclI T'GATC_A
Cuts at: 1548 2304 2563 1548
Size: 756 259 5274
Fragments arranged by size:
5274 756 259
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4900 136
Size: 107 122 71 631 605 3228 1525
Fragments arranged by size:
3228 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6284 6284
Size: 6289
BlpI GC'TnA_GC
Cuts at: 2378 3706 2378
Size: 1328 4961
Bme1580I G_kGCm'C
Cuts at: 1790 2169 2337 3237 4350 5506 1790
Size: 379 168 900 1113 1156 2573
Fragments arranged by size:
2573 1156 1113 900 379 168
BmgBI CAC'GTC
Cuts at: 1318 3184 1318
Size: 1866 4423
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2131 4020 4978
Size: 416 53 83 399 24 714 1889 958
Cuts at: 4978 442
Size: 1753
Fragments arranged by size:
1889 1753 958 714 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2339 2469 2943 4949 1239
Size: 1100 130 474 2006 2579
Fragments arranged by size:
2579 2006 1100 474 130
Bpu10I CC'TnA_GC
Cuts at: 2454 2907 2454
Size: 453 5836
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2333 3431 4414 5279 5577 2333
Size: 1098 983 865 298 3045
Fragments arranged by size:
3045 1098 983 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2919 4952 882
Size: 617 1420 2033 2219
Fragments arranged by size:
2219 2033 1420 617
BsaAI yAC'GTr
Cuts at: 493 1204 2881 3403 4080 493
Size: 711 1677 522 677 2702
Fragments arranged by size:
2702 1677 711 677 522
BsaBI GATnn'nnATC
Cuts at: 6283 6283
Size: 6289
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4096 4478 5113 275
Size: 53 83 186 936 2563 382 635 1451
Fragments arranged by size:
2563 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4722 5120 5394 5541 5952 4722
Size: 398 274 147 411 5059
Fragments arranged by size:
5059 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2396 2426 2442 2472 1178
Size: 30 165 30 993 30 16 30 4995
Fragments arranged by size:
4995 993 165 30 30 30 30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2404 2702 3101 3315 3455 3515 1186
Size: 1218 298 399 214 140 60 3960
Fragments arranged by size:
3960 1218 399 298 214 140 60
BseYI C'CCAG_C
Cuts at: 2018 3544 5492 5988 2018
Size: 1526 1948 496 2319
Fragments arranged by size:
2319 1948 1526 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2021 2589 1163
Size: 573 285 568 4863
Fragments arranged by size:
4863 573 568 285
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3739 4500 5528 1540
Size: 275 186 1738 761 1028 2301
Fragments arranged by size:
2301 1738 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2478 2678 3402 3447 4350 4435 5506 2478
Size: 200 724 45 903 85 1071 3261
Fragments arranged by size:
3261 1071 903 724 200 85 45
BsmI GAATG_Cn'
Cuts at: 3800 3893 3800
Size: 93 6196
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2337 2478 2678 3237 3402 3447
Size: 399 379 168 141 200 559 165 45
Cuts at: 3447 3551 3608 4350 4435 5506 1391
Size: 104 57 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 559 399 379 200 168
165 141 104 85 57 45
BspEI T'CCGG_A
Cuts at: 5952 5952
Size: 6289
BspHI T'CATG_A
Cuts at: 3534 4178 5908 3534
Size: 644 1730 3915
Fragments arranged by size:
3915 1730 644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2064 2458 3644 844
Size: 1220 394 1186 3489
Fragments arranged by size:
3489 1220 1186 394
BsrBI CCG'CTC
Cuts at: 1394 1823 2104 3216 4176 1394
Size: 429 281 1112 960 3507
Fragments arranged by size:
3507 1112 960 429 281
BsrDI GCAATG_nn'
Cuts at: 65 1248 2319 4784 4958 65
Size: 1183 1071 2465 174 1396
Fragments arranged by size:
2465 1396 1183 1071 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4933 5120 1700
Size: 110 3123 187 2869
Fragments arranged by size:
3123 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2403 2914 96
Size: 2307 511 3471
Fragments arranged by size:
3471 2307 511
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6289
BssSI C'ACGA_G
Cuts at: 4349 5361 4349
Size: 1012 5277
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2482 3386 794
Size: 1688 904 3697
Fragments arranged by size:
3697 1688 904
BstBI TT'CG_AA
Cuts at: 5093 5093
Size: 6289
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6289
BstYI r'GATC_y
Cuts at: 1972 2968 3031 3619 3986 4371 4388 5840 5926
Size: 996 63 588 367 385 17 1452 86
Cuts at: 5926 5938 6284 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 996 588 385 367 346 86
63 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2136 2261 2418 3200 513
Size: 552 939 132 125 157 782 3602
Fragments arranged by size:
3602 939 782 552 157 132 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3493 487
Size: 683 56 2267 3283
Fragments arranged by size:
3283 2267 683 56
BtsI GCAGTG_nn'
Cuts at: 3805 4599 4619 3805
Size: 794 20 5475
Fragments arranged by size:
5475 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6289
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6254
DraI TTT'AAA
Cuts at: 2535 3593 2535
Size: 1058 5231
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5296 809
Size: 507 3980 1802
Fragments arranged by size:
3980 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3012 3054 3736
Size: 54 1425 50 275 18 1182 42 682
Cuts at: 3736 4625 6254 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1182 889 682 275 54 50
43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 3736 1537
Size: 275 1924 4090
Fragments arranged by size:
4090 1924 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2022 2070 2520 2679 2683 4219 1353
Size: 669 48 450 159 4 1536 3423
Fragments arranged by size:
3423 1536 669 450 159 48 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4876 5250 5396 387
Size: 1064 138 3287 374 146 1280
Fragments arranged by size:
3287 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2676 3445 2676
Size: 769 5520
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2046 2105 2117 2339 2469 2664 2759
Size: 393 414 59 12 222 130 195 95
Cuts at: 2759 2943 2951 4352 4949 5736 6004 1239
Size: 184 8 1401 597 787 268 1524
Fragments arranged by size:
1524 1401 787 597 414 393 268 222
195 184 130 95 59 12 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2060 2891 3378 2060
Size: 831 487 4971
Fragments arranged by size:
4971 831 487
EcoO109I rG'GnC_Cy
Cuts at: 3062 3062
Size: 6289
EcoRI G'AATT_C
Cuts at: 2935 3073 3598 3976 2935
Size: 138 525 378 5248
Fragments arranged by size:
5248 525 378 138
EcoRV GAT'ATC
Cuts at: 3112 3112
Size: 6289
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2764 3338 3481 2764
Size: 574 143 5572
Fragments arranged by size:
5572 574 143
FspI TGC'GCA
Cuts at: 4795 4795
Size: 6289
HaeII r_GCGC'y
Cuts at: 1678 3021 5436 1678
Size: 1343 2415 2531
Fragments arranged by size:
2531 2415 1343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4930 4962 5004 5036 1373
Size: 32 135 32 3358 32 42 32 2626
Fragments arranged by size:
3358 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3633 3879 669
Size: 988 281 1695 246 3079
Fragments arranged by size:
3079 1695 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6289
HpaI GTT'AAC
Cuts at: 3879 3879
Size: 6289
KpnI G_GTAC'C
Cuts at: 3614 3614
Size: 6289
MfeI C'AATT_G
Cuts at: 3888 3888
Size: 6289
MluI A'CGCG_T
Cuts at: 6165 6165
Size: 6289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5403 5587 1305
Size: 4098 184 2007
Fragments arranged by size:
4098 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3014 3056 10
Size: 54 1425 343 1182 42 3243
Fragments arranged by size:
3243 1425 1182 343 54 42
MslI CAynn'nnrTG
Cuts at: 518 1166 2330 3134 3150 4247 4606 4765 518
Size: 648 1164 804 16 1097 359 159 2042
Fragments arranged by size:
2042 1164 1097 804 648 359 159 16
MspA1I CmG'CkG
Cuts at: 1606 3705 4382 5530 5775 1606
Size: 2099 677 1148 245 2120
Fragments arranged by size:
2120 2099 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2261 513
Size: 552 939 257 4541
Fragments arranged by size:
4541 939 552 257
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6289
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6289
NotI GC'GGCC_GC
Cuts at: 3736 3736
Size: 6289
NspI r_CATG'y
Cuts at: 1132 1229 2153 2804 3322 3647 5192 1132
Size: 97 924 651 518 325 1545 2229
Fragments arranged by size:
2229 1545 924 651 518 325 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6289
PciI A'CATG_T
Cuts at: 2149 5188 2149
Size: 3039 3250
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6289
PfoI T'CCnGG_A
Cuts at: 2650 2920 2976 2650
Size: 270 56 5963
Fragments arranged by size:
5963 270 56
PmeI GTTT'AAAC
Cuts at: 3593 3593
Size: 6289
PmlI CAC'GTG
Cuts at: 3403 3403
Size: 6289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4358 4390 5871 5903 4358
Size: 32 1481 32 4744
Fragments arranged by size:
4744 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6289
PsiI TTA'TAA
Cuts at: 3859 3859
Size: 6289
PstI C_TGCA'G
Cuts at: 830 2061 2514 3252 3641 830
Size: 1231 453 738 389 3478
Fragments arranged by size:
3478 1231 738 453 389
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6289
SacI G_AGCT'C
Cuts at: 2678 3447 2678
Size: 769 5520
SalI G'TCGA_C
Cuts at: 1936 3631 1936
Size: 1695 4594
SapI GCTCTTCn'nnn_
Cuts at: 2683 2683
Size: 6289
SbfI CC_TGCA'GG
Cuts at: 2061 3641 2061
Size: 1580 4709
ScaI AGT'ACT
Cuts at: 4537 6249 4537
Size: 1712 4577
SfcI C'TryA_G
Cuts at: 826 1045 2057 2510 3248 3336 3637 4772 5453
Size: 219 1012 453 738 88 301 1135 681
Cuts at: 5453 5644 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1012 738 681 453 301 219
191 88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6289
SmaI CCC'GGG
Cuts at: 1872 3616 1872
Size: 1744 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2348 2816 3446 4393 5294 5556
Size: 197 926 405 468 630 947 901 262
Cuts at: 5556 820
Size: 1553
Fragments arranged by size:
1553 947 926 901 630 468 405 262
197
SnaBI TAC'GTA
Cuts at: 493 4080 493
Size: 3587 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6289
SphI G_CATG'C
Cuts at: 1132 3322 3647 1132
Size: 2190 325 3774
Fragments arranged by size:
3774 2190 325
SspI AAT'ATT
Cuts at: 5 52 4213 5
Size: 47 4161 2081
Fragments arranged by size:
4161 2081 47
StuI AGG'CCT
Cuts at: 1517 2747 1517
Size: 1230 5059
StyI C'CwwG_G
Cuts at: 513 1065 2004 2261 2337 3237 3313 3728 513
Size: 552 939 257 76 900 76 415 3074
Fragments arranged by size:
3074 939 900 552 415 257 76 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3162 4316 4333 4486 4671 5116 325
Size: 1255 1582 1154 17 153 185 445 1498
Fragments arranged by size:
1582 1498 1255 1154 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2388 2403 2914
Size: 274 80 33 51 1443 411 15 511
Cuts at: 2914 4535 6247 96
Size: 1621 1712 138
Fragments arranged by size:
1712 1621 1443 511 411 274 138 80
51 33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2442 2474 6249 6281 2442
Size: 32 3775 32 2450
Fragments arranged by size:
3775 2450 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6289
XbaI T'CTAG_A
Cuts at: 2272 3625 2272
Size: 1353 4936
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6289
XmaI C'CCGG_G
Cuts at: 1870 3614 1870
Size: 1744 4545
XmnI GAAnn'nnTTC
Cuts at: 2747 3464 4418 2747
Size: 717 954 4618
Fragments arranged by size:
4618 954 717
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4097 276
Size: 53 83 186 936 2563 2468
Fragments arranged by size:
2563 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI