Restriction map for
pFN21AE4503
(Circular) (Six-base) MAPSORT of: pFN21AE4503.seq Check: 5655 from: 1 to: 6289
pFN21AE4503 6289 bp
With 182 enzymes: SgfI *
March 28, 2012 14:05 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4099 278
Size: 53 83 186 936 2563 2468
Fragments arranged by size:
2563 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2377 2868 3632 1937
Size: 440 491 764 4594
Fragments arranged by size:
4594 764 491 440
Acc65I G'GTAC_C
Cuts at: 3610 3610
Size: 6289
AclI AA'CG_TT
Cuts at: 1563 4416 4789 1563
Size: 2853 373 3063
Fragments arranged by size:
3063 2853 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2182 3277 3320 3570 4352 5736 6004 1632
Size: 550 1095 43 250 782 1384 268 1917
Fragments arranged by size:
1917 1384 1095 782 550 268 250 43
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6092
AflIII A'CryG_T
Cuts at: 1203 2141 5188 6165 1203
Size: 938 3047 977 1327
Fragments arranged by size:
3047 1327 977 938
AgeI A'CCGG_T
Cuts at: 5120 5120
Size: 6289
AhdI GACnn_n'nnGTC
Cuts at: 5018 5018
Size: 6289
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6289
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6289
AlwNI CAG_nnn'CTG
Cuts at: 4032 5604 4032
Size: 1572 4717
ApaI G_GGCC'C
Cuts at: 2694 3030 3413 3534 2694
Size: 336 383 121 5449
Fragments arranged by size:
5449 383 336 121
ApaLI G'TGCA_C
Cuts at: 4346 5502 4346
Size: 1156 5133
ApoI r'AATT_y
Cuts at: 1493 3598 3824 3976 6085 1493
Size: 2105 226 152 2109 1697
Fragments arranged by size:
2109 2105 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4843 160
Size: 872 3811 1606
Fragments arranged by size:
3811 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
AvaI C'yCGr_G
Cuts at: 1870 1943 2016 2425 2446 3189 3614 1870
Size: 73 73 409 21 743 425 4545
Fragments arranged by size:
4545 743 425 409 73 73 21
BamHI G'GATC_C
Cuts at: 2285 3619 3986 2285
Size: 1334 367 4588
Fragments arranged by size:
4588 1334 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 2111 2789 3281 3446
Size: 325 217 627 233 91 678 492 165
Cuts at: 3446 3610 5065 618
Size: 164 1455 1842
Fragments arranged by size:
1842 1455 678 627 492 325 233 217
165 164 91
BanII G_rGCy'C
Cuts at: 1391 2012 2694 3030 3161 3266 3413 3534 3608
Size: 621 682 336 131 105 147 121 74
Cuts at: 3608 1391
Size: 4072
Fragments arranged by size:
4072 682 621 336 147 131 121 105
74
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5318
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3075 3109 4480 4514 1903
Size: 34 1138 34 1371 34 3678
Fragments arranged by size:
3678 1371 1138 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2623 4181 5391 2623
Size: 1558 1210 3521
Fragments arranged by size:
3521 1558 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6289
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2838 3417 3529
Size: 107 122 71 631 605 1166 579 112
Cuts at: 3529 4900 136
Size: 1371 1525
Fragments arranged by size:
1525 1371 1166 631 605 579 122 112
107 71
BglII A'GATC_T
Cuts at: 6284 6284
Size: 6289
BlpI GC'TnA_GC
Cuts at: 3706 3706
Size: 6289
Bme1580I G_kGCm'C
Cuts at: 1790 2023 2049 2694 2794 3030 3413 3534 4350
Size: 233 26 645 100 236 383 121 816
Cuts at: 4350 5506 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 816 645 383 236 233 121
100 26
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6289
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2184 3414 4020
Size: 416 53 83 399 24 767 1230 606
Cuts at: 4020 4978 442
Size: 958 1753
Fragments arranged by size:
1753 1230 958 767 606 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3542 1056
Size: 2486 3803
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3593
Size: 6289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2173 2200 2251 2263 2817 2985 3040 3166
Size: 934 27 51 12 554 168 55 126
Cuts at: 3166 3289 3448 4949 1239
Size: 123 159 1501 2579
Fragments arranged by size:
2579 1501 934 554 168 159 126 123
55 51 27 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4414 5279 5577 4414
Size: 865 298 5126
Fragments arranged by size:
5126 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2081 4952 882
Size: 617 582 2871 2219
Fragments arranged by size:
2871 2219 617 582
BsaAI yAC'GTr
Cuts at: 493 1204 4080 493
Size: 711 2876 2702
Fragments arranged by size:
2876 2702 711
BsaBI GATnn'nnATC
Cuts at: 6283 6283
Size: 6289
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4096 4478 5113 275
Size: 53 83 186 936 2563 382 635 1451
Fragments arranged by size:
2563 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2360 2390 3224 4722 5120 5394 5541 5952 2360
Size: 30 834 1498 398 274 147 411 2697
Fragments arranged by size:
2697 1498 834 411 398 274 147 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2198 2228 1178
Size: 30 165 30 795 30 5239
Fragments arranged by size:
5239 795 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 2347 2896 3379 1186
Size: 883 278 549 483 4096
Fragments arranged by size:
4096 883 549 483 278
BseYI C'CCAG_C
Cuts at: 2400 2404 2571 2723 3023 3074 3197 3534 5492
Size: 4 167 152 300 51 123 337 1958
Cuts at: 5492 5988 2400
Size: 496 2701
Fragments arranged by size:
2701 1958 496 337 300 167 152 123
51 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5716
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3739 4500 5528 1540
Size: 275 186 1738 761 1028 2301
Fragments arranged by size:
2301 1738 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4350 4435 5506 4350
Size: 85 1071 5133
Fragments arranged by size:
5133 1071 85
BsmI GAATG_Cn'
Cuts at: 3104 3800 3893 3104
Size: 696 93 5500
Fragments arranged by size:
5500 696 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2019 2704 2019
Size: 685 5604
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2023 2049 2694 2794 3030 3161
Size: 399 222 11 26 645 100 236 131
Cuts at: 3161 3266 3413 3534 3608 4350 4435 5506 1391
Size: 105 147 121 74 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 645 399 236 222 147
131 121 105 100 85 74 26 11
BspEI T'CCGG_A
Cuts at: 2360 5952 2360
Size: 3592 2697
BspHI T'CATG_A
Cuts at: 4178 5908 4178
Size: 1730 4559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3644 844
Size: 2800 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3492 4176 1394
Size: 429 1669 684 3507
Fragments arranged by size:
3507 1669 684 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2292 2830 3099 4784 4958 65
Size: 1183 1044 538 269 1685 174 1396
Fragments arranged by size:
1685 1396 1183 1044 538 269 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3284 4933 5120 1700
Size: 110 1474 1649 187 2869
Fragments arranged by size:
2869 1649 1474 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6289
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6289
BssSI C'ACGA_G
Cuts at: 4349 5361 4349
Size: 1012 5277
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6289
BstBI TT'CG_AA
Cuts at: 5093 5093
Size: 6289
BstEII G'GTnAC_C
Cuts at: 1184 3368 1184
Size: 2184 4105
BstXI CCAn_nnnn'nTGG
Cuts at: 2606 3140 3320 2606
Size: 534 180 5575
Fragments arranged by size:
5575 534 180
BstYI r'GATC_y
Cuts at: 1972 2285 2315 2363 3480 3619 3986 4371 4388
Size: 313 30 48 1117 139 367 385 17
Cuts at: 4388 5840 5926 5938 6284 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1117 385 367 346 313 139
86 48 30 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2551 3153 513
Size: 552 939 547 602 3649
Fragments arranged by size:
3649 939 602 552 547
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5550
Fragments arranged by size:
5550 683 56
BtsI GCAGTG_nn'
Cuts at: 2060 2626 2689 3256 3475 3805 4599 4619 2060
Size: 566 63 567 219 330 794 20 3730
Fragments arranged by size:
3730 794 567 566 330 219 63 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6289
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3439 3474 529
Size: 35 2875 35 3344
Fragments arranged by size:
3344 2875 35 35
DraI TTT'AAA
Cuts at: 3593 3593
Size: 6289
DraIII CAC_nnn'GTG
Cuts at: 2737 2737
Size: 6289
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5296 809
Size: 507 3980 1802
Fragments arranged by size:
3980 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2623 3096 3736
Size: 54 1425 50 275 18 793 473 640
Cuts at: 3736 4625 6254 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 793 640 473 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3736 1537
Size: 275 1924 4090
Fragments arranged by size:
4090 1924 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2593 2599 3367 4219 1353
Size: 1240 6 768 852 3423
Fragments arranged by size:
3423 1240 852 768 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4876 5250 5396 387
Size: 1064 138 3287 374 146 1280
Fragments arranged by size:
3287 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2173 2182 2200 2251 2263 2817 2985
Size: 393 541 9 18 51 12 554 168
Cuts at: 2985 3040 3166 3277 3289 3320 3448 3570 4352
Size: 55 126 111 12 31 128 122 782
Cuts at: 4352 4949 5736 6004 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 782 597 554 541 393 268
168 128 126 122 111 55 51 31
18 12 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2991 2991
Size: 6289
EcoO109I rG'GnC_Cy
Cuts at: 2091 2134 2690 2810 3007 3027 3167 3409 3410
Size: 43 556 120 197 20 140 242 1
Cuts at: 3410 3530 3531 2091
Size: 120 1 4849
Fragments arranged by size:
4849 556 242 197 140 120 120 43
20 1 1
EcoRI G'AATT_C
Cuts at: 3598 3976 3598
Size: 378 5911
EcoRV GAT'ATC
Cuts at: 3505 3505
Size: 6289
FspI TGC'GCA
Cuts at: 4795 4795
Size: 6289
HaeII r_GCGC'y
Cuts at: 1678 5436 1678
Size: 3758 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2679 2711 4930 4962 5004
Size: 32 135 32 1107 32 2219 32 42
Cuts at: 5004 5036 1373
Size: 32 2626
Fragments arranged by size:
2626 2219 1107 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3633 3879 669
Size: 988 281 1695 246 3079
Fragments arranged by size:
3079 1695 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6289
HpaI GTT'AAC
Cuts at: 3879 3879
Size: 6289
KpnI G_GTAC'C
Cuts at: 3614 3614
Size: 6289
MfeI C'AATT_G
Cuts at: 3888 3888
Size: 6289
MluI A'CGCG_T
Cuts at: 2141 6165 2141
Size: 4024 2265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2664 5403 5587 1305
Size: 1359 2739 184 2007
Fragments arranged by size:
2739 2007 1359 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2625 10
Size: 54 1425 343 793 3674
Fragments arranged by size:
3674 1425 793 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4247 4606 4765 518
Size: 648 3081 359 159 2042
Fragments arranged by size:
3081 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2193 2283 2404 2436 2553 3023 3443 3705
Size: 587 90 121 32 117 470 420 262
Cuts at: 3705 4382 5530 5775 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 587 470 420 262 245
121 117 90 32
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4798
Fragments arranged by size:
4798 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6289
NheI G'CTAG_C
Cuts at: 1052 3538 1052
Size: 2486 3803
NotI GC'GGCC_GC
Cuts at: 3736 3736
Size: 6289
NspI r_CATG'y
Cuts at: 1132 1229 3382 3647 5192 1132
Size: 97 2153 265 1545 2229
Fragments arranged by size:
2229 2153 1545 265 97
PasI CC'CwG_GG
Cuts at: 1348 2299 1348
Size: 951 5338
PciI A'CATG_T
Cuts at: 5188 5188
Size: 6289
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3115 1108
Size: 2007 4282
PfoI T'CCnGG_A
Cuts at: 3498 3498
Size: 6289
PmeI GTTT'AAAC
Cuts at: 3593 3593
Size: 6289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4358 4390 5871 5903 4358
Size: 32 1481 32 4744
Fragments arranged by size:
4744 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2091 2134 2810 3007 3167 2091
Size: 43 676 197 160 5213
Fragments arranged by size:
5213 676 197 160 43
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6289
PsiI TTA'TAA
Cuts at: 3859 3859
Size: 6289
PspOMI G'GGCC_C
Cuts at: 2690 3026 3409 3530 2690
Size: 336 383 121 5449
Fragments arranged by size:
5449 383 336 121
PstI C_TGCA'G
Cuts at: 830 2314 2684 3641 830
Size: 1484 370 957 3478
Fragments arranged by size:
3478 1484 957 370
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6289
PvuII CAG'CTG
Cuts at: 2404 3023 2404
Size: 619 5670
SacII CC_GC'GG
Cuts at: 2554 2554
Size: 6289
SalI G'TCGA_C
Cuts at: 1936 3631 1936
Size: 1695 4594
SanDI GG'GwC_CC
Cuts at: 3007 3167 3007
Size: 160 6129
SapI GCTCTTCn'nnn_
Cuts at: 2593 3367 2593
Size: 774 5515
SbfI CC_TGCA'GG
Cuts at: 3641 3641
Size: 6289
ScaI AGT'ACT
Cuts at: 4537 6249 4537
Size: 1712 4577
SfcI C'TryA_G
Cuts at: 826 1045 2206 2310 2653 2680 2818 2865 2938
Size: 219 1161 104 343 27 138 47 73
Cuts at: 2938 3637 4772 5453 5644 826
Size: 699 1135 681 191 1471
Fragments arranged by size:
1471 1161 1135 699 681 343 219 191
138 104 73 47 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6289
SmaI CCC'GGG
Cuts at: 1872 3616 1872
Size: 1744 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3189 4393 5294 5556 820
Size: 197 926 1246 1204 901 262 1553
Fragments arranged by size:
1553 1246 1204 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4080 493
Size: 3587 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6289
SphI G_CATG'C
Cuts at: 1132 3647 1132
Size: 2515 3774
SspI AAT'ATT
Cuts at: 5 52 4213 5
Size: 47 4161 2081
Fragments arranged by size:
4161 2081 47
StuI AGG'CCT
Cuts at: 1517 2755 1517
Size: 1238 5051
StyI C'CwwG_G
Cuts at: 513 1065 2004 2094 3162 3565 3728 513
Size: 552 939 90 1068 403 163 3074
Fragments arranged by size:
3074 1068 939 552 403 163 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2030 2888 4316 4333 4486 4671 5116
Size: 1255 450 858 1428 17 153 185 445
Cuts at: 5116 325
Size: 1498
Fragments arranged by size:
1498 1428 1255 858 450 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4535 6247 96
Size: 274 80 33 51 1443 2558 1712 138
Fragments arranged by size:
2558 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2020 2052 6249 6281 2020
Size: 32 4197 32 2028
Fragments arranged by size:
4197 2028 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6289
XbaI T'CTAG_A
Cuts at: 3625 3625
Size: 6289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2513 2783 2513
Size: 270 6019
XhoI C'TCGA_G
Cuts at: 1943 3189 1943
Size: 1246 5043
XmaI C'CCGG_G
Cuts at: 1870 3614 1870
Size: 1744 4545
XmnI GAAnn'nnTTC
Cuts at: 4418 4418
Size: 6289
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4097 276
Size: 53 83 186 936 2563 2468
Fragments arranged by size:
2563 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SexAI SfiI SfoI
SrfI SwaI