Restriction map for
pFN21AE4496
(Circular) (Six-base) MAPSORT of: pFN21AE4496.seq Check: 5713 from: 1 to: 6268
pFN21AE4496 6268 bp
With 182 enzymes: SgfI *
March 28, 2012 13:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4078 278
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2784 3611 1937
Size: 847 827 4594
Fragments arranged by size:
4594 847 827
Acc65I G'GTAC_C
Cuts at: 3589 3589
Size: 6268
AclI AA'CG_TT
Cuts at: 1563 4395 4768 1563
Size: 2832 373 3063
Fragments arranged by size:
3063 2832 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3351 3447 4331 5715 5983 1632
Size: 1719 96 884 1384 268 1917
Fragments arranged by size:
1917 1719 1384 884 268 96
AflII C'TTAA_G
Cuts at: 820 1017 2653 820
Size: 197 1636 4435
Fragments arranged by size:
4435 1636 197
AflIII A'CryG_T
Cuts at: 1203 5167 6144 1203
Size: 3964 977 1327
Fragments arranged by size:
3964 1327 977
AgeI A'CCGG_T
Cuts at: 5099 5099
Size: 6268
AhdI GACnn_n'nnGTC
Cuts at: 4997 4997
Size: 6268
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6268
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6268
AlwNI CAG_nnn'CTG
Cuts at: 4011 5583 4011
Size: 1572 4696
ApaI G_GGCC'C
Cuts at: 2571 2800 2571
Size: 229 6039
ApaLI G'TGCA_C
Cuts at: 4325 5481 4325
Size: 1156 5112
ApoI r'AATT_y
Cuts at: 1493 2044 2262 3577 3803 3955 6064 1493
Size: 551 218 1315 226 152 2109 1697
Fragments arranged by size:
2109 1697 1315 551 226 218 152
AseI AT'TA_AT
Cuts at: 160 1032 3030 4822 160
Size: 872 1998 1792 1606
Fragments arranged by size:
1998 1792 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
AvaI C'yCGr_G
Cuts at: 1870 1943 3593 1870
Size: 73 1650 4545
Fragments arranged by size:
4545 1650 73
BamHI G'GATC_C
Cuts at: 3598 3965 3598
Size: 367 5901
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3589 5044 618
Size: 325 217 627 1802 1455 1842
Fragments arranged by size:
1842 1802 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2571 2800 3587 1391
Size: 1180 229 787 4072
Fragments arranged by size:
4072 1180 787 229
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2089 2184 3423 3486 928
Size: 971 190 95 1239 63 3710
Fragments arranged by size:
3710 1239 971 190 95 63
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4459 4493 1903
Size: 34 2522 34 3678
Fragments arranged by size:
3678 2522 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4160 5370 4160
Size: 1210 5058
BclI T'GATC_A
Cuts at: 1548 3448 1548
Size: 1900 4368
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4879 136
Size: 107 122 71 631 605 3207 1525
Fragments arranged by size:
3207 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6263 6263
Size: 6268
BlpI GC'TnA_GC
Cuts at: 2882 3685 2882
Size: 803 5465
Bme1580I G_kGCm'C
Cuts at: 1790 2571 2800 4329 5485 1790
Size: 781 229 1529 1156 2573
Fragments arranged by size:
2573 1529 1156 781 229
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6268
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2566 3999 4957
Size: 416 53 83 399 24 1149 1433 958
Cuts at: 4957 442
Size: 1753
Fragments arranged by size:
1753 1433 1149 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3401 4928 1239
Size: 2162 1527 2579
Fragments arranged by size:
2579 2162 1527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2024 2439 4393 5258 5556 2024
Size: 415 1954 865 298 2736
Fragments arranged by size:
2736 1954 865 415 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4931 882
Size: 617 3432 2219
Fragments arranged by size:
3432 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4059 493
Size: 711 2855 2702
Fragments arranged by size:
2855 2702 711
BsaBI GATnn'nnATC
Cuts at: 2133 2313 2385 3105 6262 2133
Size: 180 72 720 3157 2139
Fragments arranged by size:
3157 2139 720 180 72
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4075 4457 5092 275
Size: 53 83 186 936 2542 382 635 1451
Fragments arranged by size:
2542 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4701 5099 5373 5520 5931 4701
Size: 398 274 147 411 5038
Fragments arranged by size:
5038 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6043
Fragments arranged by size:
6043 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6268
BseYI C'CCAG_C
Cuts at: 2998 5471 5967 2998
Size: 2473 496 3299
Fragments arranged by size:
3299 2473 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2295 2898 1163
Size: 573 559 603 4533
Fragments arranged by size:
4533 603 573 559
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3718 4479 5507 1540
Size: 275 186 1717 761 1028 2301
Fragments arranged by size:
2301 1717 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4329 4414 5485 4329
Size: 85 1071 5112
Fragments arranged by size:
5112 1071 85
BsmI GAATG_Cn'
Cuts at: 3779 3872 3779
Size: 93 6175
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2571 2800 3587 4329 4414 5485 1391
Size: 399 781 229 787 742 85 1071 2174
Fragments arranged by size:
2174 1071 787 781 742 399 229 85
BspEI T'CCGG_A
Cuts at: 5931 5931
Size: 6268
BspHI T'CATG_A
Cuts at: 3406 4157 5887 3406
Size: 751 1730 3787
Fragments arranged by size:
3787 1730 751
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3073 3623 844
Size: 2229 550 3489
Fragments arranged by size:
3489 2229 550
BsrBI CCG'CTC
Cuts at: 1394 1823 4155 1394
Size: 429 2332 3507
Fragments arranged by size:
3507 2332 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2427 2830 4763 4937 65
Size: 1183 1179 403 1933 174 1396
Fragments arranged by size:
1933 1396 1183 1179 403 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4912 5099 1700
Size: 110 3102 187 2869
Fragments arranged by size:
3102 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6268
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6268
BssSI C'ACGA_G
Cuts at: 4328 5340 4328
Size: 1012 5256
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6268
BstBI TT'CG_AA
Cuts at: 5072 5072
Size: 6268
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6268
BstYI r'GATC_y
Cuts at: 1972 3598 3965 4350 4367 5819 5905 5917 6263
Size: 1626 367 385 17 1452 86 12 346
Cuts at: 6263 1972
Size: 1977
Fragments arranged by size:
1977 1626 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2492 2492
Size: 6268
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4777
Fragments arranged by size:
4777 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5529
Fragments arranged by size:
5529 683 56
BtsI GCAGTG_nn'
Cuts at: 2541 3048 3063 3784 4578 4598 2541
Size: 507 15 721 794 20 4211
Fragments arranged by size:
4211 794 721 507 20 15
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6268
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2519 2554 529
Size: 35 1955 35 4243
Fragments arranged by size:
4243 1955 35 35
DraI TTT'AAA
Cuts at: 3572 3572
Size: 6268
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5275 809
Size: 507 3959 1802
Fragments arranged by size:
3959 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3715 4604 6233
Size: 54 1425 50 275 18 1885 889 1629
Cuts at: 6233 8
Size: 43
Fragments arranged by size:
1885 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3715 1537
Size: 275 1903 4090
Fragments arranged by size:
4090 1903 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3466 4198 1353
Size: 2113 732 3423
Fragments arranged by size:
3423 2113 732
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 3528 4855 5229 5375 387
Size: 1064 138 435 1504 1327 374 146 1280
Fragments arranged by size:
1504 1327 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3351 3401 3447 4331 4928 5715 5983
Size: 393 1719 50 46 884 597 787 268
Cuts at: 5983 1239
Size: 1524
Fragments arranged by size:
1719 1524 884 787 597 393 268 50
46
EcoO109I rG'GnC_Cy
Cuts at: 2568 2797 2568
Size: 229 6039
EcoRI G'AATT_C
Cuts at: 2262 3577 3955 2262
Size: 1315 378 4575
Fragments arranged by size:
4575 1315 378
EcoRV GAT'ATC
Cuts at: 2170 2170
Size: 6268
FspI TGC'GCA
Cuts at: 4774 4774
Size: 6268
HaeII r_GCGC'y
Cuts at: 1678 5415 1678
Size: 3737 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2571 2603 3433 3465 4909
Size: 32 135 32 999 32 830 32 1444
Cuts at: 4909 4941 4983 5015 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1444 999 830 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2680 3612 3858 669
Size: 988 281 742 932 246 3079
Fragments arranged by size:
3079 988 932 742 281 246
HindIII A'AGCT_T
Cuts at: 748 2294 748
Size: 1546 4722
HpaI GTT'AAC
Cuts at: 3858 3858
Size: 6268
KpnI G_GTAC'C
Cuts at: 3593 3593
Size: 6268
MfeI C'AATT_G
Cuts at: 2322 3867 2322
Size: 1545 4723
MluI A'CGCG_T
Cuts at: 6144 6144
Size: 6268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3081 3253 5382 5566 1305
Size: 1776 172 2129 184 2007
Fragments arranged by size:
2129 2007 1776 184 172
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4446
Fragments arranged by size:
4446 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2517 2766 3411 4226 4585 4744 518
Size: 648 1351 249 645 815 359 159 2042
Fragments arranged by size:
2042 1351 815 648 645 359 249 159
MspA1I CmG'CkG
Cuts at: 1606 3684 4361 5509 5754 1606
Size: 2078 677 1148 245 2120
Fragments arranged by size:
2120 2078 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4777
Fragments arranged by size:
4777 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6268
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6268
NotI GC'GGCC_GC
Cuts at: 3715 3715
Size: 6268
NspI r_CATG'y
Cuts at: 1132 1229 3626 5171 1132
Size: 97 2397 1545 2229
Fragments arranged by size:
2397 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6268
PciI A'CATG_T
Cuts at: 5167 5167
Size: 6268
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6268
PmeI GTTT'AAAC
Cuts at: 3572 3572
Size: 6268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4337 4369 5850 5882 4337
Size: 32 1481 32 4723
Fragments arranged by size:
4723 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6268
PsiI TTA'TAA
Cuts at: 3838 3838
Size: 6268
PspOMI G'GGCC_C
Cuts at: 2567 2796 2567
Size: 229 6039
PstI C_TGCA'G
Cuts at: 830 3070 3620 830
Size: 2240 550 3478
Fragments arranged by size:
3478 2240 550
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6268
SalI G'TCGA_C
Cuts at: 1936 3610 1936
Size: 1674 4594
SbfI CC_TGCA'GG
Cuts at: 3620 3620
Size: 6268
ScaI AGT'ACT
Cuts at: 3297 4516 6228 3297
Size: 1219 1712 3337
Fragments arranged by size:
3337 1712 1219
SfcI C'TryA_G
Cuts at: 826 1045 3066 3616 4751 5432 5623 826
Size: 219 2021 550 1135 681 191 1471
Fragments arranged by size:
2021 1471 1135 681 550 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6268
SmaI CCC'GGG
Cuts at: 1872 3595 1872
Size: 1723 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2039 2454 2653 4372 5273 5535
Size: 197 926 96 415 199 1719 901 262
Cuts at: 5535 820
Size: 1553
Fragments arranged by size:
1719 1553 926 901 415 262 199 197
96
SnaBI TAC'GTA
Cuts at: 493 4059 493
Size: 3566 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6268
SphI G_CATG'C
Cuts at: 1132 3626 1132
Size: 2494 3774
SspI AAT'ATT
Cuts at: 5 52 2349 4192 5
Size: 47 2297 1843 2081
Fragments arranged by size:
2297 2081 1843 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6268
StyI C'CwwG_G
Cuts at: 513 1065 2004 2257 3707 513
Size: 552 939 253 1450 3074
Fragments arranged by size:
3074 1450 939 552 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4295 4312 4465 4650 5095 325
Size: 1255 2715 17 153 185 445 1498
Fragments arranged by size:
2715 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3295 4514 6226
Size: 274 80 33 51 1443 1318 1219 1712
Cuts at: 6226 96
Size: 138
Fragments arranged by size:
1712 1443 1318 1219 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6228 6260 6228
Size: 32 6236
Tth111I GACn'n_nGTC
Cuts at: 1316 2789 1316
Size: 1473 4795
XbaI T'CTAG_A
Cuts at: 2221 3604 2221
Size: 1383 4885
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6268
XmaI C'CCGG_G
Cuts at: 1870 3593 1870
Size: 1723 4545
XmnI GAAnn'nnTTC
Cuts at: 2721 3396 4397 2721
Size: 675 1001 4592
Fragments arranged by size:
4592 1001 675
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4076 276
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI