Restriction map for
pFN21AE4491
(Circular) (Six-base) MAPSORT of: pFN21AE4491.seq Check: 5956 from: 1 to: 6241
pFN21AE4491 6241 bp
With 182 enzymes: SgfI *
March 28, 2012 13:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4051 278
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3584 1937
Size: 1647 4594
Acc65I G'GTAC_C
Cuts at: 3562 3562
Size: 6241
AclI AA'CG_TT
Cuts at: 1563 4368 4741 1563
Size: 2805 373 3063
Fragments arranged by size:
3063 2805 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2135 2356 4304 5688 5956 1632
Size: 503 221 1948 1384 268 1917
Fragments arranged by size:
1948 1917 1384 503 268 221
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6044
AflIII A'CryG_T
Cuts at: 1203 5140 6117 1203
Size: 3937 977 1327
Fragments arranged by size:
3937 1327 977
AgeI A'CCGG_T
Cuts at: 5072 5072
Size: 6241
AhdI GACnn_n'nnGTC
Cuts at: 2099 4970 2099
Size: 2871 3370
AleI CACnn'nnGTG
Cuts at: 1166 2249 1166
Size: 1083 5158
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6241
AlwNI CAG_nnn'CTG
Cuts at: 2290 2384 2533 2546 3984 5556 2290
Size: 94 149 13 1438 1572 2975
Fragments arranged by size:
2975 1572 1438 149 94 13
ApaLI G'TGCA_C
Cuts at: 4298 5454 4298
Size: 1156 5085
ApoI r'AATT_y
Cuts at: 1493 3440 3550 3776 3928 6037 1493
Size: 1947 110 226 152 2109 1697
Fragments arranged by size:
2109 1947 1697 226 152 110
AseI AT'TA_AT
Cuts at: 160 1032 4795 160
Size: 872 3763 1606
Fragments arranged by size:
3763 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
AvaI C'yCGr_G
Cuts at: 1870 1943 3566 1870
Size: 73 1623 4545
Fragments arranged by size:
4545 1623 73
BamHI G'GATC_C
Cuts at: 3571 3938 3571
Size: 367 5874
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2409 3562 5017 618
Size: 325 217 627 622 1153 1455 1842
Fragments arranged by size:
1842 1455 1153 627 622 325 217
BanII G_rGCy'C
Cuts at: 1391 3560 1391
Size: 2169 4072
BbeI G_GCGC'C
Cuts at: 2413 2413
Size: 6241
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5270
BbvCI CC'TCA_GC
Cuts at: 2505 2505
Size: 6241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4432 4466 1903
Size: 34 50 34 2411 34 3678
Fragments arranged by size:
3678 2411 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4133 5343 4133
Size: 1210 5031
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6241
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4852 136
Size: 107 122 71 631 605 3180 1525
Fragments arranged by size:
3180 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6236 6236
Size: 6241
BlpI GC'TnA_GC
Cuts at: 2261 2648 3658 2261
Size: 387 1010 4844
Fragments arranged by size:
4844 1010 387
Bme1580I G_kGCm'C
Cuts at: 1790 2157 2553 4302 5458 1790
Size: 367 396 1749 1156 2573
Fragments arranged by size:
2573 1749 1156 396 367
BmgBI CAC'GTC
Cuts at: 1318 2221 1318
Size: 903 5338
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3464 3972 4930
Size: 416 53 83 399 24 2047 508 958
Cuts at: 4930 442
Size: 1753
Fragments arranged by size:
2047 1753 958 508 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3007 3143 4901 1239
Size: 1768 136 1758 2579
Fragments arranged by size:
2579 1768 1758 136
Bpu10I CC'TnA_GC
Cuts at: 2505 2505
Size: 6241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2074 4366 5231 5529 2074
Size: 2292 865 298 2786
Fragments arranged by size:
2786 2292 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3119 3377 4904 882
Size: 617 1620 258 1527 2219
Fragments arranged by size:
2219 1620 1527 617 258
BsaAI yAC'GTr
Cuts at: 493 1204 4032 493
Size: 711 2828 2702
Fragments arranged by size:
2828 2702 711
BsaBI GATnn'nnATC
Cuts at: 6235 6235
Size: 6241
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2410 4048 4430 5065
Size: 53 83 186 936 877 1638 382 635
Cuts at: 5065 275
Size: 1451
Fragments arranged by size:
1638 1451 936 877 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4674 5072 5346 5493 5904 4674
Size: 398 274 147 411 5011
Fragments arranged by size:
5011 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2444 2474 1178
Size: 30 165 30 1041 30 4945
Fragments arranged by size:
4945 1041 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2452 3053 1186
Size: 1266 601 4374
Fragments arranged by size:
4374 1266 601
BseYI C'CCAG_C
Cuts at: 2455 2701 2954 3396 5444 5940 2455
Size: 246 253 442 2048 496 2756
Fragments arranged by size:
2756 2048 496 442 253 246
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5668
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3691 4452 5480 1540
Size: 275 186 1690 761 1028 2301
Fragments arranged by size:
2301 1690 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2811 2868 3519 4302 4387 5458 2811
Size: 57 651 783 85 1071 3594
Fragments arranged by size:
3594 1071 783 651 85 57
BsmI GAATG_Cn'
Cuts at: 2755 3752 3845 2755
Size: 997 93 5151
Fragments arranged by size:
5151 997 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2553 2811 2868 3519 3560 4302
Size: 399 367 396 258 57 651 41 742
Cuts at: 4302 4387 5458 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 651 399 396 367 258
85 57 41
BspEI T'CCGG_A
Cuts at: 5904 5904
Size: 6241
BspHI T'CATG_A
Cuts at: 4130 5860 4130
Size: 1730 4511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3051 3596 844
Size: 2207 545 3489
Fragments arranged by size:
3489 2207 545
BsrBI CCG'CTC
Cuts at: 1394 1823 2265 4128 1394
Size: 429 442 1863 3507
Fragments arranged by size:
3507 1863 442 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2442 3387 4736 4910 65
Size: 1183 1194 945 1349 174 1396
Fragments arranged by size:
1396 1349 1194 1183 945 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2319 2406 2616 4885 5072 1700
Size: 110 509 87 210 2269 187 2869
Fragments arranged by size:
2869 2269 509 210 187 110 87
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6241
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6241
BssSI C'ACGA_G
Cuts at: 4301 5313 4301
Size: 1012 5229
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6241
BstBI TT'CG_AA
Cuts at: 5045 5045
Size: 6241
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6241
BstXI CCAn_nnnn'nTGG
Cuts at: 2133 2139 2213 2251 2133
Size: 6 74 38 6123
Fragments arranged by size:
6123 74 38 6
BstYI r'GATC_y
Cuts at: 1972 2229 3030 3089 3571 3938 4323 4340 5792
Size: 257 801 59 482 367 385 17 1452
Cuts at: 5792 5878 5890 6236 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 801 482 385 367 346 257
86 59 17 12
Bsu36I CC'TnA_GG
Cuts at: 2097 2097
Size: 6241
BtgI C'CryG_G
Cuts at: 513 1065 2031 2132 2250 513
Size: 552 966 101 118 4504
Fragments arranged by size:
4504 966 552 118 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2319 487
Size: 683 56 1093 4409
Fragments arranged by size:
4409 1093 683 56
BtsI GCAGTG_nn'
Cuts at: 3757 4551 4571 3757
Size: 794 20 5427
Fragments arranged by size:
5427 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6206
DraI TTT'AAA
Cuts at: 3545 3545
Size: 6241
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5248 809
Size: 507 3932 1802
Fragments arranged by size:
3932 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2614 3688
Size: 54 1425 50 275 18 198 586 1074
Cuts at: 3688 4577 6206 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1074 889 586 275 198 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3688 1537
Size: 275 1876 4090
Fragments arranged by size:
4090 1876 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2478 3145 3416 4171 1353
Size: 1125 667 271 755 3423
Fragments arranged by size:
3423 1125 755 667 271
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4828 5202 5348 387
Size: 1064 138 3239 374 146 1280
Fragments arranged by size:
3239 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2356 3007 3143 4304 4901 5688
Size: 393 503 221 651 136 1161 597 787
Cuts at: 5688 5956 1239
Size: 268 1524
Fragments arranged by size:
1524 1161 787 651 597 503 393 268
221 136
EcoO109I rG'GnC_Cy
Cuts at: 2085 2101 2287 2509 2543 2761 2085
Size: 16 186 222 34 218 5565
Fragments arranged by size:
5565 222 218 186 34 16
EcoRI G'AATT_C
Cuts at: 3440 3550 3928 3440
Size: 110 378 5753
Fragments arranged by size:
5753 378 110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2663 2663
Size: 6241
FseI GG_CCGG'CC
Cuts at: 2323 2323
Size: 6241
FspI TGC'GCA
Cuts at: 4747 4747
Size: 6241
HaeII r_GCGC'y
Cuts at: 1678 2413 2623 5388 1678
Size: 735 210 2765 2531
Fragments arranged by size:
2765 2531 735 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2085 2117 2444 2476 4882
Size: 32 135 32 513 32 327 32 2406
Cuts at: 4882 4914 4956 4988 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2406 513 327 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2659 3271 3585 3831 669
Size: 988 281 721 612 314 246 3079
Fragments arranged by size:
3079 988 721 612 314 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6241
HpaI GTT'AAC
Cuts at: 3831 3831
Size: 6241
KasI G'GCGC_C
Cuts at: 2409 2409
Size: 6241
KpnI G_GTAC'C
Cuts at: 3566 3566
Size: 6241
MfeI C'AATT_G
Cuts at: 3840 3840
Size: 6241
MluI A'CGCG_T
Cuts at: 6117 6117
Size: 6241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3157 5355 5539 1305
Size: 1852 2198 184 2007
Fragments arranged by size:
2198 2007 1852 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4419
Fragments arranged by size:
4419 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2249 4199 4558 4717 518
Size: 648 1083 1950 359 159 2042
Fragments arranged by size:
2042 1950 1083 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2705 2884 2908 3396 3532 3657 4334 5482
Size: 1099 179 24 488 136 125 677 1148
Cuts at: 5482 5727 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1099 677 488 245 179 136
125 24
NaeI GCC'GGC
Cuts at: 2321 2408 2618 2321
Size: 87 210 5944
Fragments arranged by size:
5944 210 87
NarI GG'CG_CC
Cuts at: 2410 2410
Size: 6241
NcoI C'CATG_G
Cuts at: 513 1065 2132 513
Size: 552 1067 4622
Fragments arranged by size:
4622 1067 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6241
NgoMIV G'CCGG_C
Cuts at: 2319 2406 2616 2319
Size: 87 210 5944
Fragments arranged by size:
5944 210 87
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6241
NotI GC'GGCC_GC
Cuts at: 3688 3688
Size: 6241
NspI r_CATG'y
Cuts at: 1132 1229 2161 2368 2492 3506 3599 5144 1132
Size: 97 932 207 124 1014 93 1545 2229
Fragments arranged by size:
2229 1545 1014 932 207 124 97 93
PasI CC'CwG_GG
Cuts at: 1348 2167 3015 1348
Size: 819 848 4574
Fragments arranged by size:
4574 848 819
PciI A'CATG_T
Cuts at: 5140 5140
Size: 6241
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2384 1108
Size: 1276 4965
PfoI T'CCnGG_A
Cuts at: 2838 2984 3120 2838
Size: 146 136 5959
Fragments arranged by size:
5959 146 136
PmeI GTTT'AAAC
Cuts at: 3545 3545
Size: 6241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3285 3317 4310 4342 5823 5855 3285
Size: 32 993 32 1481 32 3671
Fragments arranged by size:
3671 1481 993 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2101 2509 2761 2101
Size: 408 252 5581
Fragments arranged by size:
5581 408 252
PshAI GACnn'nnGTC
Cuts at: 1613 2591 1613
Size: 978 5263
PsiI TTA'TAA
Cuts at: 3811 3811
Size: 6241
PstI C_TGCA'G
Cuts at: 830 2229 3593 830
Size: 1399 1364 3478
Fragments arranged by size:
3478 1399 1364
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6241
PvuII CAG'CTG
Cuts at: 2705 2884 2908 3532 2705
Size: 179 24 624 5414
Fragments arranged by size:
5414 624 179 24
SalI G'TCGA_C
Cuts at: 1936 3583 1936
Size: 1647 4594
SapI GCTCTTCn'nnn_
Cuts at: 2478 2478
Size: 6241
SbfI CC_TGCA'GG
Cuts at: 3593 3593
Size: 6241
ScaI AGT'ACT
Cuts at: 4489 6201 4489
Size: 1712 4529
SfcI C'TryA_G
Cuts at: 826 1045 2225 2563 2779 3589 4724 5405 5596
Size: 219 1180 338 216 810 1135 681 191
Cuts at: 5596 826
Size: 1471
Fragments arranged by size:
1471 1180 1135 810 681 338 219 216
191
SfoI GGC'GCC
Cuts at: 2411 2411
Size: 6241
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
SgrAI Cr'CCGG_yG
Cuts at: 1700 2406 1700
Size: 706 5535
SmaI CCC'GGG
Cuts at: 1872 3568 1872
Size: 1696 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2089 4345 5246 5508 820
Size: 197 926 146 2256 901 262 1553
Fragments arranged by size:
2256 1553 926 901 262 197 146
SnaBI TAC'GTA
Cuts at: 493 4032 493
Size: 3539 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6241
SphI G_CATG'C
Cuts at: 1132 3506 3599 1132
Size: 2374 93 3774
Fragments arranged by size:
3774 2374 93
SspI AAT'ATT
Cuts at: 5 52 4165 5
Size: 47 4113 2081
Fragments arranged by size:
4113 2081 47
StuI AGG'CCT
Cuts at: 1517 2112 3113 1517
Size: 595 1001 4645
Fragments arranged by size:
4645 1001 595
StyI C'CwwG_G
Cuts at: 513 1065 2132 3210 3680 513
Size: 552 1067 1078 470 3074
Fragments arranged by size:
3074 1078 1067 552 470
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4268 4285 4438 4623 5068 325
Size: 1255 2688 17 153 185 445 1498
Fragments arranged by size:
2688 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4487 6199 96
Size: 274 80 33 51 1443 2510 1712 138
Fragments arranged by size:
2510 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6201 6233 6201
Size: 32 6209
Tth111I GACn'n_nGTC
Cuts at: 1316 2966 1316
Size: 1650 4591
XbaI T'CTAG_A
Cuts at: 3577 3577
Size: 6241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2130 2251 2130
Size: 121 6120
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6241
XmaI C'CCGG_G
Cuts at: 1870 3566 1870
Size: 1696 4545
XmnI GAAnn'nnTTC
Cuts at: 2435 2753 3297 4370 2435
Size: 318 544 1073 4306
Fragments arranged by size:
4306 1073 544 318
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4049 276
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI