Restriction map for
pFN21AE4488
(Circular) (Six-base) MAPSORT of: pFN21AE4488.seq Check: 2281 from: 1 to: 6235
pFN21AE4488 6235 bp
With 182 enzymes: SgfI *
March 28, 2012 13:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3339 3339
Size: 6235
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4045 278
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3434 3578 1937
Size: 1497 144 4594
Fragments arranged by size:
4594 1497 144
Acc65I G'GTAC_C
Cuts at: 3556 3556
Size: 6235
AclI AA'CG_TT
Cuts at: 1563 4362 4735 1563
Size: 2799 373 3063
Fragments arranged by size:
3063 2799 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4298 5682 5950 1632
Size: 2666 1384 268 1917
Fragments arranged by size:
2666 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6038
AflIII A'CryG_T
Cuts at: 1203 5134 6111 1203
Size: 3931 977 1327
Fragments arranged by size:
3931 1327 977
AgeI A'CCGG_T
Cuts at: 5066 5066
Size: 6235
AhdI GACnn_n'nnGTC
Cuts at: 4964 4964
Size: 6235
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6235
AlwNI CAG_nnn'CTG
Cuts at: 3978 5550 3978
Size: 1572 4663
ApaI G_GGCC'C
Cuts at: 2209 2411 2209
Size: 202 6033
ApaLI G'TGCA_C
Cuts at: 4292 5448 4292
Size: 1156 5079
ApoI r'AATT_y
Cuts at: 1493 2241 2687 2844 2879 2921 3226 3527 3544
Size: 748 446 157 35 42 305 301 17
Cuts at: 3544 3770 3922 6031 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 748 446 305 301 226 157
152 42 35 17
AseI AT'TA_AT
Cuts at: 160 1032 4789 160
Size: 872 3757 1606
Fragments arranged by size:
3757 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
AvaI C'yCGr_G
Cuts at: 1870 1943 3560 1870
Size: 73 1617 4545
Fragments arranged by size:
4545 1617 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3165 3198 3165
Size: 33 6202
BamHI G'GATC_C
Cuts at: 2012 3565 3932 2012
Size: 1553 367 4315
Fragments arranged by size:
4315 1553 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3556 5011 618
Size: 325 217 627 1769 1455 1842
Fragments arranged by size:
1842 1769 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2209 2411 3554 1391
Size: 818 202 1143 4072
Fragments arranged by size:
4072 1143 818 202
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5264
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4426 4460 1903
Size: 34 2489 34 3678
Fragments arranged by size:
3678 2489 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3166 4127 5337 3166
Size: 961 1210 4064
Fragments arranged by size:
4064 1210 961
BclI T'GATC_A
Cuts at: 1548 2649 1548
Size: 1101 5134
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2274 4846 136
Size: 107 122 71 631 605 602 2572 1525
Fragments arranged by size:
2572 1525 631 605 602 122 107 71
BglII A'GATC_T
Cuts at: 6230 6230
Size: 6235
BlpI GC'TnA_GC
Cuts at: 3652 3652
Size: 6235
Bme1580I G_kGCm'C
Cuts at: 1790 2199 2209 2411 4296 5452 1790
Size: 409 10 202 1885 1156 2573
Fragments arranged by size:
2573 1885 1156 409 202 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6235
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2323 3966 4924
Size: 416 53 83 399 24 906 1643 958
Cuts at: 4924 442
Size: 1753
Fragments arranged by size:
1753 1643 958 906 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6235
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3130 3130
Size: 6235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2775 3303 4895 1239
Size: 1536 528 1592 2579
Fragments arranged by size:
2579 1592 1536 528
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3253 4360 5225 5523 3253
Size: 1107 865 298 3965
Fragments arranged by size:
3965 1107 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2033 2103 3507 4898 882
Size: 617 534 70 1404 1391 2219
Fragments arranged by size:
2219 1404 1391 617 534 70
BsaAI yAC'GTr
Cuts at: 493 1204 4026 493
Size: 711 2822 2702
Fragments arranged by size:
2822 2702 711
BsaBI GATnn'nnATC
Cuts at: 6229 6229
Size: 6235
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3288 4042 4424 5059
Size: 53 83 186 936 1755 754 382 635
Cuts at: 5059 275
Size: 1451
Fragments arranged by size:
1755 1451 936 754 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4668 5066 5340 5487 5898 4668
Size: 398 274 147 411 5005
Fragments arranged by size:
5005 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6010
Fragments arranged by size:
6010 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2121 3138 1186
Size: 935 1017 4283
Fragments arranged by size:
4283 1017 935
BseYI C'CCAG_C
Cuts at: 5438 5934 5438
Size: 496 5739
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 2706 3317 3386 1163
Size: 573 462 508 611 69 4012
Fragments arranged by size:
4012 611 573 508 462 69
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2048 2080 2121 3685 4446 5474
Size: 275 186 47 32 41 1564 761 1028
Cuts at: 5474 1540
Size: 2301
Fragments arranged by size:
2301 1564 1028 761 275 186 47 41
32
BsiHKAI G_wGCw'C
Cuts at: 2889 3330 4296 4381 5452 2889
Size: 441 966 85 1071 3672
Fragments arranged by size:
3672 1071 966 441 85
BsmI GAATG_Cn'
Cuts at: 2224 3138 3267 3746 3839 2224
Size: 914 129 479 93 4620
Fragments arranged by size:
4620 914 479 129 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2199 2209 2411 2889 3330 3554 4296
Size: 399 409 10 202 478 441 224 742
Cuts at: 4296 4381 5452 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 478 441 409 399 224
202 85 10
BspEI T'CCGG_A
Cuts at: 5898 5898
Size: 6235
BspHI T'CATG_A
Cuts at: 4124 5854 4124
Size: 1730 4505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3339 3590 844
Size: 2495 251 3489
Fragments arranged by size:
3489 2495 251
BsrBI CCG'CTC
Cuts at: 1394 1823 4122 1394
Size: 429 2299 3507
Fragments arranged by size:
3507 2299 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4730 4904 65
Size: 1183 3482 174 1396
Fragments arranged by size:
3482 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3051 4879 5066 1700
Size: 110 1241 1828 187 2869
Fragments arranged by size:
2869 1828 1241 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6235
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6235
BssSI C'ACGA_G
Cuts at: 4295 5307 4295
Size: 1012 5223
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2176 794
Size: 1382 4853
BstBI TT'CG_AA
Cuts at: 5039 5039
Size: 6235
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6235
BstYI r'GATC_y
Cuts at: 1972 2012 3565 3932 4317 4334 5786 5872 5884
Size: 40 1553 367 385 17 1452 86 12
Cuts at: 5884 6230 1972
Size: 346 1977
Fragments arranged by size:
1977 1553 1452 385 367 346 86 40
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2115 513
Size: 552 939 111 4633
Fragments arranged by size:
4633 939 552 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2117 487
Size: 683 56 891 4605
Fragments arranged by size:
4605 891 683 56
BtsI GCAGTG_nn'
Cuts at: 3751 4545 4565 3751
Size: 794 20 5421
Fragments arranged by size:
5421 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6200
DraI TTT'AAA
Cuts at: 2262 3539 2262
Size: 1277 4958
DraIII CAC_nnn'GTG
Cuts at: 2176 2176
Size: 6235
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2117 2777 5242 809
Size: 507 801 660 2465 1802
Fragments arranged by size:
2465 1802 801 660 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2045 3682 4571
Size: 54 1425 50 275 18 215 1637 889
Cuts at: 4571 6200 8
Size: 1629 43
Fragments arranged by size:
1637 1629 1425 889 275 215 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2045 3682 1537
Size: 275 233 1637 4090
Fragments arranged by size:
4090 1637 275 233
EarI CTCTTCn'nnn_
Cuts at: 1353 2341 3100 4165 1353
Size: 988 759 1065 3423
Fragments arranged by size:
3423 1065 988 759
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4822 5196 5342 387
Size: 1064 138 3233 374 146 1280
Fragments arranged by size:
3233 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2775 3303 4298 4895 5682 5950 1239
Size: 393 1143 528 995 597 787 268 1524
Fragments arranged by size:
1524 1143 995 787 597 528 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2724 2724
Size: 6235
EcoO109I rG'GnC_Cy
Cuts at: 2407 2408 2407
Size: 1 6234
EcoRI G'AATT_C
Cuts at: 2921 3544 3922 2921
Size: 623 378 5234
Fragments arranged by size:
5234 623 378
FspI TGC'GCA
Cuts at: 4741 4741
Size: 6235
HaeII r_GCGC'y
Cuts at: 1678 5382 1678
Size: 3704 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4876 4908 4950 4982 1373
Size: 32 135 32 3304 32 42 32 2626
Fragments arranged by size:
3304 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2805 3579 3825 669
Size: 988 281 867 774 246 3079
Fragments arranged by size:
3079 988 867 774 281 246
HindIII A'AGCT_T
Cuts at: 748 2559 748
Size: 1811 4424
HpaI GTT'AAC
Cuts at: 3825 3825
Size: 6235
KpnI G_GTAC'C
Cuts at: 3560 3560
Size: 6235
MfeI C'AATT_G
Cuts at: 3834 3834
Size: 6235
MluI A'CGCG_T
Cuts at: 6111 6111
Size: 6235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2425 5349 5533 1305
Size: 1120 2924 184 2007
Fragments arranged by size:
2924 2007 1120 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4413
Fragments arranged by size:
4413 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2541 4193 4552 4711 518
Size: 648 1375 1652 359 159 2042
Fragments arranged by size:
2042 1652 1375 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2117 2426 3046 3518 3651 4328 5476
Size: 444 67 309 620 472 133 677 1148
Cuts at: 5476 5721 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 620 472 444 309 245
133 67
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4744
Fragments arranged by size:
4744 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6235
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6235
NotI GC'GGCC_GC
Cuts at: 3682 3682
Size: 6235
NspI r_CATG'y
Cuts at: 1132 1229 3593 5138 1132
Size: 97 2364 1545 2229
Fragments arranged by size:
2364 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6235
PciI A'CATG_T
Cuts at: 5134 5134
Size: 6235
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6235
PmeI GTTT'AAAC
Cuts at: 3539 3539
Size: 6235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4304 4336 5817 5849 4304
Size: 32 1481 32 4690
Fragments arranged by size:
4690 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6235
PsiI TTA'TAA
Cuts at: 3805 3805
Size: 6235
PspOMI G'GGCC_C
Cuts at: 2205 2407 2205
Size: 202 6033
PstI C_TGCA'G
Cuts at: 830 3587 830
Size: 2757 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6235
PvuII CAG'CTG
Cuts at: 2426 3046 3518 2426
Size: 620 472 5143
Fragments arranged by size:
5143 620 472
SacII CC_GC'GG
Cuts at: 2118 2118
Size: 6235
SalI G'TCGA_C
Cuts at: 1936 3577 1936
Size: 1641 4594
SbfI CC_TGCA'GG
Cuts at: 3587 3587
Size: 6235
ScaI AGT'ACT
Cuts at: 4483 6195 4483
Size: 1712 4523
SexAI A'CCwGG_T
Cuts at: 2172 2172
Size: 6235
SfcI C'TryA_G
Cuts at: 826 1045 2496 3583 4718 5399 5590 826
Size: 219 1451 1087 1135 681 191 1471
Fragments arranged by size:
1471 1451 1135 1087 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6235
SmaI CCC'GGG
Cuts at: 1872 3562 1872
Size: 1690 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3232 4339 5240 5502 820
Size: 197 926 1289 1107 901 262 1553
Fragments arranged by size:
1553 1289 1107 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4026 493
Size: 3533 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6235
SphI G_CATG'C
Cuts at: 1132 3593 1132
Size: 2461 3774
SspI AAT'ATT
Cuts at: 5 52 2451 2970 4159 5
Size: 47 2399 519 1189 2081
Fragments arranged by size:
2399 2081 1189 519 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6235
StyI C'CwwG_G
Cuts at: 513 1065 2004 2947 3674 513
Size: 552 939 943 727 3074
Fragments arranged by size:
3074 943 939 727 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2066 4262 4279 4432 4617 5062 325
Size: 1255 486 2196 17 153 185 445 1498
Fragments arranged by size:
2196 1498 1255 486 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 4481 6193
Size: 274 80 33 51 1443 168 2336 1712
Cuts at: 6193 96
Size: 138
Fragments arranged by size:
2336 1712 1443 274 168 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6195 6227 6195
Size: 32 6203
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6235
XbaI T'CTAG_A
Cuts at: 3571 3571
Size: 6235
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6235
XmaI C'CCGG_G
Cuts at: 1870 3560 1870
Size: 1690 4545
XmnI GAAnn'nnTTC
Cuts at: 4364 4364
Size: 6235
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4043 276
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI
SanDI SapI SfiI SfoI SrfI SwaI XcmI