Restriction map for
pFN21AE4487
(Circular) (Six-base) MAPSORT of: pFN21AE4487.seq Check: 4888 from: 1 to: 6229
pFN21AE4487 6229 bp
With 182 enzymes: SgfI *
March 28, 2012 13:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3168 4039 278
Size: 53 83 186 936 1632 871 2468
Fragments arranged by size:
2468 1632 936 871 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3572 1937
Size: 1635 4594
Acc65I G'GTAC_C
Cuts at: 3550 3550
Size: 6229
AclI AA'CG_TT
Cuts at: 1563 4356 4729 1563
Size: 2793 373 3063
Fragments arranged by size:
3063 2793 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2546 4292 5676 5944 1632
Size: 914 1746 1384 268 1917
Fragments arranged by size:
1917 1746 1384 914 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6032
AflIII A'CryG_T
Cuts at: 1203 5128 6105 1203
Size: 3925 977 1327
Fragments arranged by size:
3925 1327 977
AgeI A'CCGG_T
Cuts at: 5060 5060
Size: 6229
AhdI GACnn_n'nnGTC
Cuts at: 4958 4958
Size: 6229
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6229
AlwNI CAG_nnn'CTG
Cuts at: 2083 2308 2368 3972 5544 2083
Size: 225 60 1604 1572 2768
Fragments arranged by size:
2768 1604 1572 225 60
ApaLI G'TGCA_C
Cuts at: 4286 5442 4286
Size: 1156 5073
ApoI r'AATT_y
Cuts at: 1493 2162 2804 2997 3052 3359 3538 3764 3916
Size: 669 642 193 55 307 179 226 152
Cuts at: 3916 6025 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 669 642 307 226 193 179
152 55
AseI AT'TA_AT
Cuts at: 160 1032 2493 4783 160
Size: 872 1461 2290 1606
Fragments arranged by size:
2290 1606 1461 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
AvaI C'yCGr_G
Cuts at: 1870 1943 3554 1870
Size: 73 1611 4545
Fragments arranged by size:
4545 1611 73
BamHI G'GATC_C
Cuts at: 3559 3926 3559
Size: 367 5862
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3550 5005 618
Size: 325 217 627 1763 1455 1842
Fragments arranged by size:
1842 1763 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2523 3548 1391
Size: 622 510 1025 4072
Fragments arranged by size:
4072 1025 622 510
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5258
BbvCI CC'TCA_GC
Cuts at: 3464 3464
Size: 6229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4420 4454 1903
Size: 34 2483 34 3678
Fragments arranged by size:
3678 2483 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2868 4121 5331 2868
Size: 1253 1210 3766
Fragments arranged by size:
3766 1253 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6229
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4840 136
Size: 107 122 71 631 605 3168 1525
Fragments arranged by size:
3168 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2439 6224 2439
Size: 3785 2444
BlpI GC'TnA_GC
Cuts at: 3646 3646
Size: 6229
Bme1580I G_kGCm'C
Cuts at: 1790 4290 5446 1790
Size: 2500 1156 2573
Fragments arranged by size:
2573 2500 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6229
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2636 3960 4918
Size: 416 53 83 399 24 1219 1324 958
Cuts at: 4918 442
Size: 1753
Fragments arranged by size:
1753 1324 1219 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 4889 1239
Size: 866 2784 2579
Fragments arranged by size:
2784 2579 866
Bpu10I CC'TnA_GC
Cuts at: 3464 3464
Size: 6229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2240 4354 5219 5517 2240
Size: 2114 865 298 2952
Fragments arranged by size:
2952 2114 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3404 4892 882
Size: 617 1905 1488 2219
Fragments arranged by size:
2219 1905 1488 617
BsaAI yAC'GTr
Cuts at: 493 1204 4020 493
Size: 711 2816 2702
Fragments arranged by size:
2816 2702 711
BsaBI GATnn'nnATC
Cuts at: 2967 6223 2967
Size: 3256 2973
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3165 4036 4418 5053
Size: 53 83 186 936 1632 871 382 635
Cuts at: 5053 275
Size: 1451
Fragments arranged by size:
1632 1451 936 871 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4662 5060 5334 5481 5892 4662
Size: 398 274 147 411 4999
Fragments arranged by size:
4999 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3384 3414 1178
Size: 30 165 30 1981 30 3993
Fragments arranged by size:
3993 1981 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3219 3481 1186
Size: 2033 262 3934
Fragments arranged by size:
3934 2033 262
BseYI C'CCAG_C
Cuts at: 5432 5928 5432
Size: 496 5733
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2126 1163
Size: 573 390 5266
Fragments arranged by size:
5266 573 390
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2050 2054 3679 4440 5468 1540
Size: 275 186 49 4 1625 761 1028 2301
Fragments arranged by size:
2301 1625 1028 761 275 186 49 4
BsiHKAI G_wGCw'C
Cuts at: 2013 2271 2523 4290 4375 5446 2013
Size: 258 252 1767 85 1071 2796
Fragments arranged by size:
2796 1767 1071 258 252 85
BsmI GAATG_Cn'
Cuts at: 3740 3833 3740
Size: 93 6136
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2271 2523 3548 4290 4375 5446
Size: 399 223 258 252 1025 742 85 1071
Cuts at: 5446 1391
Size: 2174
Fragments arranged by size:
2174 1071 1025 742 399 258 252 223
85
BspEI T'CCGG_A
Cuts at: 5892 5892
Size: 6229
BspHI T'CATG_A
Cuts at: 4118 5848 4118
Size: 1730 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2732 3584 844
Size: 1888 852 3489
Fragments arranged by size:
3489 1888 852
BsrBI CCG'CTC
Cuts at: 1394 1823 4116 1394
Size: 429 2293 3507
Fragments arranged by size:
3507 2293 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2727 3166 4724 4898 65
Size: 1183 1479 439 1558 174 1396
Fragments arranged by size:
1558 1479 1396 1183 439 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2049 4873 5060 1700
Size: 110 239 2824 187 2869
Fragments arranged by size:
2869 2824 239 187 110
BsrGI T'GTAC_A
Cuts at: 96 2172 96
Size: 2076 4153
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6229
BssSI C'ACGA_G
Cuts at: 4289 5301 4289
Size: 1012 5217
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2393 794
Size: 1599 4630
BstBI TT'CG_AA
Cuts at: 5033 5033
Size: 6229
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6229
BstXI CCAn_nnnn'nTGG
Cuts at: 2443 3223 2443
Size: 780 5449
BstYI r'GATC_y
Cuts at: 1972 2439 2476 3559 3926 4311 4328 5780 5866
Size: 467 37 1083 367 385 17 1452 86
Cuts at: 5866 5878 6224 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1083 467 385 367 346 86
37 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2514 513
Size: 552 939 510 4228
Fragments arranged by size:
4228 939 552 510
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2122 2365 487
Size: 683 56 896 243 4351
Fragments arranged by size:
4351 896 683 243 56
BtsI GCAGTG_nn'
Cuts at: 2562 3745 4539 4559 2562
Size: 1183 794 20 4232
Fragments arranged by size:
4232 1183 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6194
DraI TTT'AAA
Cuts at: 3294 3533 3294
Size: 239 5990
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5236 809
Size: 507 3920 1802
Fragments arranged by size:
3920 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2047 2133 2716
Size: 54 1425 50 275 18 217 86 583
Cuts at: 2716 3676 4565 6194 8
Size: 960 889 1629 43
Fragments arranged by size:
1629 1425 960 889 583 275 217 86
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2047 3676 1537
Size: 275 235 1629 4090
Fragments arranged by size:
4090 1629 275 235
EarI CTCTTCn'nnn_
Cuts at: 1353 4159 1353
Size: 2806 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4816 5190 5336 387
Size: 1064 138 3227 374 146 1280
Fragments arranged by size:
3227 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2011 2521 2011
Size: 510 5719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2546 4292 4889 5676 5944 1239
Size: 393 473 441 1746 597 787 268 1524
Fragments arranged by size:
1746 1524 787 597 473 441 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2089 3475 2089
Size: 1386 4843
EcoRI G'AATT_C
Cuts at: 2162 3538 3916 2162
Size: 1376 378 4475
Fragments arranged by size:
4475 1376 378
EcoRV GAT'ATC
Cuts at: 2125 3323 2125
Size: 1198 5031
FspI TGC'GCA
Cuts at: 4735 4735
Size: 6229
HaeII r_GCGC'y
Cuts at: 1678 5376 1678
Size: 3698 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2839 2871 3122 3154 4870
Size: 32 135 32 1267 32 251 32 1716
Cuts at: 4870 4902 4944 4976 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1716 1267 251 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3573 3819 669
Size: 988 281 1635 246 3079
Fragments arranged by size:
3079 1635 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2450 748
Size: 1702 4527
HpaI GTT'AAC
Cuts at: 3819 3819
Size: 6229
KpnI G_GTAC'C
Cuts at: 3554 3554
Size: 6229
MfeI C'AATT_G
Cuts at: 3828 3828
Size: 6229
MluI A'CGCG_T
Cuts at: 6105 6105
Size: 6229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2053 5343 5527 1305
Size: 748 3290 184 2007
Fragments arranged by size:
3290 2007 748 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2718 10
Size: 54 1425 343 886 3521
Fragments arranged by size:
3521 1425 886 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4187 4546 4705 518
Size: 648 3021 359 159 2042
Fragments arranged by size:
3021 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2083 3434 3645 4322 5470 5715 1606
Size: 477 1351 211 677 1148 245 2120
Fragments arranged by size:
2120 1351 1148 677 477 245 211
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4738
Fragments arranged by size:
4738 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6229
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6229
NotI GC'GGCC_GC
Cuts at: 3676 3676
Size: 6229
NruI TCG'CGA
Cuts at: 2056 2056
Size: 6229
NspI r_CATG'y
Cuts at: 1132 1229 3587 5132 1132
Size: 97 2358 1545 2229
Fragments arranged by size:
2358 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6229
PciI A'CATG_T
Cuts at: 5128 5128
Size: 6229
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6229
PmeI GTTT'AAAC
Cuts at: 3533 3533
Size: 6229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4298 4330 5811 5843 4298
Size: 32 1481 32 4684
Fragments arranged by size:
4684 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6229
PsiI TTA'TAA
Cuts at: 2405 3799 2405
Size: 1394 4835
PstI C_TGCA'G
Cuts at: 830 2079 2389 3581 830
Size: 1249 310 1192 3478
Fragments arranged by size:
3478 1249 1192 310
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6229
PvuII CAG'CTG
Cuts at: 2083 2083
Size: 6229
SacI G_AGCT'C
Cuts at: 2013 2523 2013
Size: 510 5719
SalI G'TCGA_C
Cuts at: 1936 3571 1936
Size: 1635 4594
SbfI CC_TGCA'GG
Cuts at: 3581 3581
Size: 6229
ScaI AGT'ACT
Cuts at: 2955 4477 6189 2955
Size: 1522 1712 2995
Fragments arranged by size:
2995 1712 1522
SfcI C'TryA_G
Cuts at: 826 1045 2075 2385 3577 4712 5393 5584 826
Size: 219 1030 310 1192 1135 681 191 1471
Fragments arranged by size:
1471 1192 1135 1030 681 310 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6229
SmaI CCC'GGG
Cuts at: 1872 3556 1872
Size: 1684 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2219 4333 5234 5496 820
Size: 197 926 276 2114 901 262 1553
Fragments arranged by size:
2114 1553 926 901 276 262 197
SnaBI TAC'GTA
Cuts at: 493 4020 493
Size: 3527 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6229
SphI G_CATG'C
Cuts at: 1132 3587 1132
Size: 2455 3774
SspI AAT'ATT
Cuts at: 5 52 4153 5
Size: 47 4101 2081
Fragments arranged by size:
4101 2081 47
StuI AGG'CCT
Cuts at: 1517 2288 1517
Size: 771 5458
StyI C'CwwG_G
Cuts at: 513 1065 2004 2197 2979 3668 513
Size: 552 939 193 782 689 3074
Fragments arranged by size:
3074 939 782 689 552 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3386 4256 4273 4426 4611 5056 325
Size: 1255 1806 870 17 153 185 445 1498
Fragments arranged by size:
1806 1498 1255 870 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2172 2953 4475
Size: 274 80 33 51 1443 195 781 1522
Cuts at: 4475 6187 96
Size: 1712 138
Fragments arranged by size:
1712 1522 1443 781 274 195 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2382 2414 6189 6221 2382
Size: 32 3775 32 2390
Fragments arranged by size:
3775 2390 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6229
XbaI T'CTAG_A
Cuts at: 3565 3565
Size: 6229
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6229
XmaI C'CCGG_G
Cuts at: 1870 3554 1870
Size: 1684 4545
XmnI GAAnn'nnTTC
Cuts at: 3377 4358 3377
Size: 981 5248
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3166 4037 276
Size: 53 83 186 936 1632 871 2468
Fragments arranged by size:
2468 1632 936 871 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NruI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI