Restriction map for
pFN21AE4485
(Circular) (Six-base) MAPSORT of: pFN21AE4485.seq Check: 4927 from: 1 to: 6208
pFN21AE4485 6208 bp
With 182 enzymes: SgfI *
March 28, 2012 13:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4018 278
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3551 1937
Size: 1614 4594
Acc65I G'GTAC_C
Cuts at: 3529 3529
Size: 6208
AclI AA'CG_TT
Cuts at: 1563 4335 4708 1563
Size: 2772 373 3063
Fragments arranged by size:
3063 2772 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4271 5655 5923 1632
Size: 2639 1384 268 1917
Fragments arranged by size:
2639 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6011
AflIII A'CryG_T
Cuts at: 1203 2416 5107 6084 1203
Size: 1213 2691 977 1327
Fragments arranged by size:
2691 1327 1213 977
AgeI A'CCGG_T
Cuts at: 5039 5039
Size: 6208
AhdI GACnn_n'nnGTC
Cuts at: 4937 4937
Size: 6208
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6208
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6208
AlwNI CAG_nnn'CTG
Cuts at: 3951 5523 3951
Size: 1572 4636
ApaLI G'TGCA_C
Cuts at: 4265 5421 4265
Size: 1156 5052
ApoI r'AATT_y
Cuts at: 1493 2312 2976 3017 3517 3743 3895 6004 1493
Size: 819 664 41 500 226 152 2109 1697
Fragments arranged by size:
2109 1697 819 664 500 226 152 41
AseI AT'TA_AT
Cuts at: 160 1032 4762 160
Size: 872 3730 1606
Fragments arranged by size:
3730 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
AvaI C'yCGr_G
Cuts at: 1870 1943 3533 1870
Size: 73 1590 4545
Fragments arranged by size:
4545 1590 73
BamHI G'GATC_C
Cuts at: 3538 3905 3538
Size: 367 5841
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3529 4984 618
Size: 325 217 627 1742 1455 1842
Fragments arranged by size:
1842 1742 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3292 3394 3527 1391
Size: 1901 102 133 4072
Fragments arranged by size:
4072 1901 133 102
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2581 2835 3105 928
Size: 971 682 254 270 4031
Fragments arranged by size:
4031 971 682 270 254
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3443 3477 4399 4433 1903
Size: 34 50 34 1422 34 922 34 3678
Fragments arranged by size:
3678 1422 922 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2527 4100 5310 2527
Size: 1573 1210 3425
Fragments arranged by size:
3425 1573 1210
BclI T'GATC_A
Cuts at: 1548 2436 1548
Size: 888 5320
BfrBI ATG'CAT
Cuts at: 2735 2735
Size: 6208
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4819 136
Size: 107 122 71 631 605 3147 1525
Fragments arranged by size:
3147 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6203 6203
Size: 6208
BlpI GC'TnA_GC
Cuts at: 3625 3625
Size: 6208
Bme1580I G_kGCm'C
Cuts at: 1790 4269 5425 1790
Size: 2479 1156 2573
Fragments arranged by size:
2573 2479 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6208
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3939 4897 442
Size: 416 53 83 399 24 2522 958 1753
Fragments arranged by size:
2522 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2582 3099 4868 1239
Size: 1343 517 1769 2579
Fragments arranged by size:
2579 1769 1343 517
Bpu10I CC'TnA_GC
Cuts at: 3068 3068
Size: 6208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4333 5198 5496 4333
Size: 865 298 5045
Fragments arranged by size:
5045 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2280 4871 882
Size: 617 781 2591 2219
Fragments arranged by size:
2591 2219 781 617
BsaAI yAC'GTr
Cuts at: 493 1204 2192 3999 493
Size: 711 988 1807 2702
Fragments arranged by size:
2702 1807 988 711
BsaBI GATnn'nnATC
Cuts at: 3183 6202 3183
Size: 3019 3189
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4015 4397 5032 275
Size: 53 83 186 936 2482 382 635 1451
Fragments arranged by size:
2482 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4641 5039 5313 5460 5871 4641
Size: 398 274 147 411 4978
Fragments arranged by size:
4978 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2688 2718 1178
Size: 30 165 30 1285 30 4668
Fragments arranged by size:
4668 1285 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2709 3282 3387 1186
Size: 1523 573 105 4007
Fragments arranged by size:
4007 1523 573 105
BseYI C'CCAG_C
Cuts at: 2009 5411 5907 2009
Size: 3402 496 2310
Fragments arranged by size:
3402 2310 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5635
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3658 4419 5447 1540
Size: 275 186 1657 761 1028 2301
Fragments arranged by size:
2301 1657 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3113 3292 4269 4354 5425 3113
Size: 179 977 85 1071 3896
Fragments arranged by size:
3896 1071 977 179 85
BsmI GAATG_Cn'
Cuts at: 3719 3812 3719
Size: 93 6115
BsmBI CGTCTCn'nnnn_
Cuts at: 2600 2600
Size: 6208
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3113 3292 3394 3527 4269 4354 5425
Size: 399 1323 179 102 133 742 85 1071
Cuts at: 5425 1391
Size: 2174
Fragments arranged by size:
2174 1323 1071 742 399 179 133 102
85
BspEI T'CCGG_A
Cuts at: 5871 5871
Size: 6208
BspHI T'CATG_A
Cuts at: 3261 4097 5827 3261
Size: 836 1730 3642
Fragments arranged by size:
3642 1730 836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3563 844
Size: 2719 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2825 4095 1394
Size: 429 1002 1270 3507
Fragments arranged by size:
3507 1270 1002 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4703 4877 65
Size: 1183 3455 174 1396
Fragments arranged by size:
3455 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4852 5039 1700
Size: 110 3042 187 2869
Fragments arranged by size:
3042 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2393 96
Size: 2297 3911
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6208
BssSI C'ACGA_G
Cuts at: 3483 4268 5280 3483
Size: 785 1012 4411
Fragments arranged by size:
4411 1012 785
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6208
BstBI TT'CG_AA
Cuts at: 5012 5012
Size: 6208
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6208
BstYI r'GATC_y
Cuts at: 1972 3538 3905 4290 4307 5759 5845 5857 6203
Size: 1566 367 385 17 1452 86 12 346
Cuts at: 6203 1972
Size: 1977
Fragments arranged by size:
1977 1566 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2490 2490
Size: 6208
BtgI C'CryG_G
Cuts at: 513 1065 2045 2931 513
Size: 552 980 886 3790
Fragments arranged by size:
3790 980 886 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5469
Fragments arranged by size:
5469 683 56
BtsI GCAGTG_nn'
Cuts at: 3724 4518 4538 3724
Size: 794 20 5394
Fragments arranged by size:
5394 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6173
DraI TTT'AAA
Cuts at: 2725 3512 2725
Size: 787 5421
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2552 5215 809
Size: 507 1236 2663 1802
Fragments arranged by size:
2663 1802 1236 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3655 4544 6173
Size: 54 1425 50 275 18 1825 889 1629
Cuts at: 6173 8
Size: 43
Fragments arranged by size:
1825 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3655 1537
Size: 275 1843 4090
Fragments arranged by size:
4090 1843 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2499 2509 2624 4138 1353
Size: 1146 10 115 1514 3423
Fragments arranged by size:
3423 1514 1146 115 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2081 4795 5169 5315 387
Size: 1064 138 492 2714 374 146 1280
Fragments arranged by size:
2714 1280 1064 492 374 146 138
EcoICRI GAG'CTC
Cuts at: 3290 3290
Size: 6208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2582 3099 4271 4868 5655 5923 1239
Size: 393 950 517 1172 597 787 268 1524
Fragments arranged by size:
1524 1172 950 787 597 517 393 268
EcoRI G'AATT_C
Cuts at: 3517 3895 3517
Size: 378 5830
FspI TGC'GCA
Cuts at: 4714 4714
Size: 6208
HaeII r_GCGC'y
Cuts at: 1678 2016 2022 5355 1678
Size: 338 6 3333 2531
Fragments arranged by size:
3333 2531 338 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4849 4881 4923 4955 1373
Size: 32 135 32 3277 32 42 32 2626
Fragments arranged by size:
3277 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2527 3552 3798 669
Size: 988 281 589 1025 246 3079
Fragments arranged by size:
3079 1025 988 589 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6208
HpaI GTT'AAC
Cuts at: 2527 3798 2527
Size: 1271 4937
KpnI G_GTAC'C
Cuts at: 3533 3533
Size: 6208
MfeI C'AATT_G
Cuts at: 3807 3807
Size: 6208
MluI A'CGCG_T
Cuts at: 6084 6084
Size: 6208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3267 5322 5506 1305
Size: 1962 2055 184 2007
Fragments arranged by size:
2055 2007 1962 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4386
Fragments arranged by size:
4386 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2879 3405 4166 4525 4684 518
Size: 648 1713 526 761 359 159 2042
Fragments arranged by size:
2042 1713 761 648 526 359 159
MspA1I CmG'CkG
Cuts at: 1606 2047 2470 2681 3624 4301 5449 5694 1606
Size: 441 423 211 943 677 1148 245 2120
Fragments arranged by size:
2120 1148 943 677 441 423 245 211
NcoI C'CATG_G
Cuts at: 513 1065 2931 513
Size: 552 1866 3790
Fragments arranged by size:
3790 1866 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6208
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6208
NotI GC'GGCC_GC
Cuts at: 3655 3655
Size: 6208
NsiI A_TGCA'T
Cuts at: 2737 2737
Size: 6208
NspI r_CATG'y
Cuts at: 1132 1229 2420 3566 5111 1132
Size: 97 1191 1146 1545 2229
Fragments arranged by size:
2229 1545 1191 1146 97
PacI TTA_AT'TAA
Cuts at: 2637 2637
Size: 6208
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6208
PciI A'CATG_T
Cuts at: 2416 5107 2416
Size: 2691 3517
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6208
PmeI GTTT'AAAC
Cuts at: 3512 3512
Size: 6208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2124 2156 4277 4309 5790 5822 2124
Size: 32 2121 32 1481 32 2510
Fragments arranged by size:
2510 2121 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6208
PsiI TTA'TAA
Cuts at: 3778 3778
Size: 6208
PstI C_TGCA'G
Cuts at: 830 2469 3560 830
Size: 1639 1091 3478
Fragments arranged by size:
3478 1639 1091
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6208
PvuII CAG'CTG
Cuts at: 2470 2681 2470
Size: 211 5997
SacI G_AGCT'C
Cuts at: 3292 3292
Size: 6208
SacII CC_GC'GG
Cuts at: 2048 2048
Size: 6208
SalI G'TCGA_C
Cuts at: 1936 3550 1936
Size: 1614 4594
SapI GCTCTTCn'nnn_
Cuts at: 2509 2509
Size: 6208
SbfI CC_TGCA'GG
Cuts at: 3560 3560
Size: 6208
ScaI AGT'ACT
Cuts at: 4456 6168 4456
Size: 1712 4496
SfcI C'TryA_G
Cuts at: 826 1045 2465 3556 4691 5372 5563 826
Size: 219 1420 1091 1135 681 191 1471
Fragments arranged by size:
1471 1420 1135 1091 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6208
SmaI CCC'GGG
Cuts at: 1872 3535 1872
Size: 1663 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4312 5213 5475 820
Size: 197 926 2369 901 262 1553
Fragments arranged by size:
2369 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3999 493
Size: 3506 2702
SpeI A'CTAG_T
Cuts at: 152 2576 152
Size: 2424 3784
SphI G_CATG'C
Cuts at: 1132 3566 1132
Size: 2434 3774
SspI AAT'ATT
Cuts at: 5 52 2943 3047 3279 4132 5
Size: 47 2891 104 232 853 2081
Fragments arranged by size:
2891 2081 853 232 104 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6208
StyI C'CwwG_G
Cuts at: 513 1065 2931 3647 513
Size: 552 1866 716 3074
Fragments arranged by size:
3074 1866 716 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4235 4252 4405 4590 5035 325
Size: 1255 2655 17 153 185 445 1498
Fragments arranged by size:
2655 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2393 4454 6166
Size: 274 80 33 51 1443 416 2061 1712
Cuts at: 6166 96
Size: 138
Fragments arranged by size:
2061 1712 1443 416 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6168 6200 6168
Size: 32 6176
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6208
XbaI T'CTAG_A
Cuts at: 3544 3544
Size: 6208
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6208
XmaI C'CCGG_G
Cuts at: 1870 3533 1870
Size: 1663 4545
XmnI GAAnn'nnTTC
Cuts at: 2098 2798 2964 4337 2098
Size: 700 166 1373 3969
Fragments arranged by size:
3969 1373 700 166
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4016 276
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PacI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BstXI BstZ17I DraIII EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI