Restriction map for
pFN21AE4476
(Circular) (Six-base) MAPSORT of: pFN21AE4476.seq Check: 4585 from: 1 to: 6190
pFN21AE4476 6190 bp
With 182 enzymes: SgfI *
March 28, 2012 13:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4000 278
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3533 1937
Size: 1596 4594
Acc65I G'GTAC_C
Cuts at: 2851 3511 2851
Size: 660 5530
AclI AA'CG_TT
Cuts at: 1563 4317 4690 1563
Size: 2754 373 3063
Fragments arranged by size:
3063 2754 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4253 5637 5905 1632
Size: 2621 1384 268 1917
Fragments arranged by size:
2621 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5993
AflIII A'CryG_T
Cuts at: 1203 2173 3487 5089 6066 1203
Size: 970 1314 1602 977 1327
Fragments arranged by size:
1602 1327 1314 977 970
AgeI A'CCGG_T
Cuts at: 5021 5021
Size: 6190
AhdI GACnn_n'nnGTC
Cuts at: 4919 4919
Size: 6190
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6190
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2326 2358 2326
Size: 32 6158
AlwNI CAG_nnn'CTG
Cuts at: 3933 5505 3933
Size: 1572 4618
ApaI G_GGCC'C
Cuts at: 3327 3327
Size: 6190
ApaLI G'TGCA_C
Cuts at: 4247 5403 4247
Size: 1156 5034
ApoI r'AATT_y
Cuts at: 1493 2775 3073 3099 3499 3725 3877 5986 1493
Size: 1282 298 26 400 226 152 2109 1697
Fragments arranged by size:
2109 1697 1282 400 298 226 152 26
AseI AT'TA_AT
Cuts at: 160 1032 3402 4744 160
Size: 872 2370 1342 1606
Fragments arranged by size:
2370 1606 1342 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
AvaI C'yCGr_G
Cuts at: 1870 1943 3515 1870
Size: 73 1572 4545
Fragments arranged by size:
4545 1572 73
BamHI G'GATC_C
Cuts at: 2430 3520 3887 2430
Size: 1090 367 4733
Fragments arranged by size:
4733 1090 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 2851 3413 3511 4966
Size: 325 217 627 291 773 562 98 1455
Cuts at: 4966 618
Size: 1842
Fragments arranged by size:
1842 1455 773 627 562 325 291 217
98
BanII G_rGCy'C
Cuts at: 1391 2575 3327 3463 3509 1391
Size: 1184 752 136 46 4072
Fragments arranged by size:
4072 1184 752 136 46
BbeI G_GCGC'C
Cuts at: 2082 2082
Size: 6190
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2556 2683 928
Size: 971 657 127 4435
Fragments arranged by size:
4435 971 657 127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4381 4415 1903
Size: 34 2444 34 3678
Fragments arranged by size:
3678 2444 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3284 4082 5292 3284
Size: 798 1210 4182
Fragments arranged by size:
4182 1210 798
BclI T'GATC_A
Cuts at: 1548 3469 1548
Size: 1921 4269
BfrBI ATG'CAT
Cuts at: 2168 2551 2168
Size: 383 5807
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2087 4801 136
Size: 107 122 71 631 605 415 2714 1525
Fragments arranged by size:
2714 1525 631 605 415 122 107 71
BglII A'GATC_T
Cuts at: 6185 6185
Size: 6190
BlpI GC'TnA_GC
Cuts at: 3607 3607
Size: 6190
Bme1580I G_kGCm'C
Cuts at: 1790 3327 4251 5407 1790
Size: 1537 924 1156 2573
Fragments arranged by size:
2573 1537 1156 924
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6190
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3218 3466 3921
Size: 416 53 83 399 24 1801 248 455
Cuts at: 3921 4879 442
Size: 958 1753
Fragments arranged by size:
1801 1753 958 455 416 399 248 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2085 2400 4850 1239
Size: 846 315 2450 2579
Fragments arranged by size:
2579 2450 846 315
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2310 4315 5180 5478 2310
Size: 2005 865 298 3022
Fragments arranged by size:
3022 2005 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2016 4853 882
Size: 617 517 2837 2219
Fragments arranged by size:
2837 2219 617 517
BsaAI yAC'GTr
Cuts at: 493 1204 2174 3981 493
Size: 711 970 1807 2702
Fragments arranged by size:
2702 1807 970 711
BsaBI GATnn'nnATC
Cuts at: 6184 6184
Size: 6190
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 3997 4379 5014
Size: 53 83 186 936 546 1918 382 635
Cuts at: 5014 275
Size: 1451
Fragments arranged by size:
1918 1451 936 635 546 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2014 4623 5021 5295 5442 5853 2014
Size: 2609 398 274 147 411 2351
Fragments arranged by size:
2609 2351 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2373 2403 2517 2547 1178
Size: 30 165 30 970 30 114 30 4821
Fragments arranged by size:
4821 970 165 114 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 1186
Size: 874 5316
BseYI C'CCAG_C
Cuts at: 5393 5889 5393
Size: 496 5694
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5617
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3640 4401 5429 1540
Size: 275 186 1639 761 1028 2301
Fragments arranged by size:
2301 1639 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2054 2512 2623 4251 4336 5407 2054
Size: 458 111 1628 85 1071 2837
Fragments arranged by size:
2837 1628 1071 458 111 85
BsmI GAATG_Cn'
Cuts at: 2260 3701 3794 2260
Size: 1441 93 4656
Fragments arranged by size:
4656 1441 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2512 2575 2623 3327 3463 3509
Size: 399 264 458 63 48 704 136 46
Cuts at: 3509 4251 4336 5407 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 704 458 399 264 136
85 63 48 46
BspEI T'CCGG_A
Cuts at: 5853 5853
Size: 6190
BspHI T'CATG_A
Cuts at: 4079 5809 4079
Size: 1730 4460
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3136 3545 844
Size: 2292 409 3489
Fragments arranged by size:
3489 2292 409
BsrBI CCG'CTC
Cuts at: 1394 1823 2099 4077 1394
Size: 429 276 1978 3507
Fragments arranged by size:
3507 1978 429 276
BsrDI GCAATG_nn'
Cuts at: 65 1248 2466 2999 3330 4685 4859 65
Size: 1183 1218 533 331 1355 174 1396
Fragments arranged by size:
1396 1355 1218 1183 533 331 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4834 5021 1700
Size: 110 3024 187 2869
Fragments arranged by size:
3024 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6190
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6190
BssSI C'ACGA_G
Cuts at: 4250 5262 4250
Size: 1012 5178
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6190
BstBI TT'CG_AA
Cuts at: 4994 4994
Size: 6190
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6190
BstYI r'GATC_y
Cuts at: 1972 2430 2736 2958 3520 3887 4272 4289 5741
Size: 458 306 222 562 367 385 17 1452
Cuts at: 5741 5827 5839 6185 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 562 458 385 367 346 306
222 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2963 2963
Size: 6190
BtgI C'CryG_G
Cuts at: 513 1065 2084 2846 3475 513
Size: 552 1019 762 629 3228
Fragments arranged by size:
3228 1019 762 629 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5451
Fragments arranged by size:
5451 683 56
BtsI GCAGTG_nn'
Cuts at: 2439 3706 4500 4520 2439
Size: 1267 794 20 4109
Fragments arranged by size:
4109 1267 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6155
DraI TTT'AAA
Cuts at: 2862 3494 2862
Size: 632 5558
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5197 809
Size: 507 3881 1802
Fragments arranged by size:
3881 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3177 3637 4526
Size: 54 1425 50 275 18 1347 460 889
Cuts at: 4526 6155 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1347 889 460 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3637 1537
Size: 275 1825 4090
Fragments arranged by size:
4090 1825 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2073 2649 3229 4120 1353
Size: 720 576 580 891 3423
Fragments arranged by size:
3423 891 720 580 576
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4777 5151 5297 387
Size: 1064 138 3188 374 146 1280
Fragments arranged by size:
3188 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2085 2400 4253 4850 5637 5905 1239
Size: 393 453 315 1853 597 787 268 1524
Fragments arranged by size:
1853 1524 787 597 453 393 315 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3009 3009
Size: 6190
EcoRI G'AATT_C
Cuts at: 3499 3877 3499
Size: 378 5812
FspI TGC'GCA
Cuts at: 4696 4696
Size: 6190
HaeII r_GCGC'y
Cuts at: 1678 2082 5337 1678
Size: 404 3255 2531
Fragments arranged by size:
3255 2531 404
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2322 2354 2415 2447 3222
Size: 32 135 32 750 32 61 32 775
Cuts at: 3222 3254 4831 4863 4905 4937 1373
Size: 32 1577 32 42 32 2626
Fragments arranged by size:
2626 1577 775 750 135 61 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3534 3780 669
Size: 988 281 1596 246 3079
Fragments arranged by size:
3079 1596 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6190
HpaI GTT'AAC
Cuts at: 3780 3780
Size: 6190
KasI G'GCGC_C
Cuts at: 2078 2078
Size: 6190
KpnI G_GTAC'C
Cuts at: 2855 3515 2855
Size: 660 5530
MfeI C'AATT_G
Cuts at: 3789 3789
Size: 6190
MluI A'CGCG_T
Cuts at: 6066 6066
Size: 6190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2467 3343 5304 5488 1305
Size: 1162 876 1961 184 2007
Fragments arranged by size:
2007 1961 1162 876 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4368
Fragments arranged by size:
4368 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4148 4507 4666 518
Size: 648 2982 359 159 2042
Fragments arranged by size:
2982 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3606 4283 5431 5676 1606
Size: 2000 677 1148 245 2120
Fragments arranged by size:
2120 2000 1148 677 245
NarI GG'CG_CC
Cuts at: 2079 2079
Size: 6190
NcoI C'CATG_G
Cuts at: 513 1065 3475 513
Size: 552 2410 3228
Fragments arranged by size:
3228 2410 552
NdeI CA'TA_TG
Cuts at: 387 2611 387
Size: 2224 3966
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6190
NotI GC'GGCC_GC
Cuts at: 3637 3637
Size: 6190
NsiI A_TGCA'T
Cuts at: 2170 2553 2170
Size: 383 5807
NspI r_CATG'y
Cuts at: 1132 1229 2253 2555 3548 5093 1132
Size: 97 1024 302 993 1545 2229
Fragments arranged by size:
2229 1545 1024 993 302 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6190
PciI A'CATG_T
Cuts at: 5089 5089
Size: 6190
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6190
PfoI T'CCnGG_A
Cuts at: 2377 3310 2377
Size: 933 5257
PmeI GTTT'AAAC
Cuts at: 3494 3494
Size: 6190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4259 4291 5772 5804 4259
Size: 32 1481 32 4645
Fragments arranged by size:
4645 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2687 1613
Size: 1074 5116
PsiI TTA'TAA
Cuts at: 3760 3760
Size: 6190
PspOMI G'GGCC_C
Cuts at: 3323 3323
Size: 6190
PstI C_TGCA'G
Cuts at: 830 2476 3133 3542 830
Size: 1646 657 409 3478
Fragments arranged by size:
3478 1646 657 409
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6190
RsrII CG'GwC_CG
Cuts at: 2056 2056
Size: 6190
SalI G'TCGA_C
Cuts at: 1936 3532 1936
Size: 1596 4594
SapI GCTCTTCn'nnn_
Cuts at: 3229 3229
Size: 6190
SbfI CC_TGCA'GG
Cuts at: 3542 3542
Size: 6190
ScaI AGT'ACT
Cuts at: 4438 6150 4438
Size: 1712 4478
SfcI C'TryA_G
Cuts at: 826 1045 2472 3129 3538 4673 5354 5545 826
Size: 219 1427 657 409 1135 681 191 1471
Fragments arranged by size:
1471 1427 1135 681 657 409 219 191
SfoI GGC'GCC
Cuts at: 2080 2080
Size: 6190
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6190
SmaI CCC'GGG
Cuts at: 1872 3517 1872
Size: 1645 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2325 4294 5195 5457 820
Size: 197 926 382 1969 901 262 1553
Fragments arranged by size:
1969 1553 926 901 382 262 197
SnaBI TAC'GTA
Cuts at: 493 3981 493
Size: 3488 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6190
SphI G_CATG'C
Cuts at: 1132 2555 3548 1132
Size: 1423 993 3774
Fragments arranged by size:
3774 1423 993
SspI AAT'ATT
Cuts at: 5 52 2718 4114 5
Size: 47 2666 1396 2081
Fragments arranged by size:
2666 2081 1396 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6190
StyI C'CwwG_G
Cuts at: 513 1065 2244 2434 3475 3629 513
Size: 552 1179 190 1041 154 3074
Fragments arranged by size:
3074 1179 1041 552 190 154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4217 4234 4387 4572 5017 325
Size: 1255 2637 17 153 185 445 1498
Fragments arranged by size:
2637 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2500 3448 4436
Size: 274 80 33 51 1443 523 948 988
Cuts at: 4436 6148 96
Size: 1712 138
Fragments arranged by size:
1712 1443 988 948 523 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2359 2391 6150 6182 2359
Size: 32 3759 32 2367
Fragments arranged by size:
3759 2367 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6190
XbaI T'CTAG_A
Cuts at: 3526 3526
Size: 6190
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6190
XmaI C'CCGG_G
Cuts at: 1870 3515 1870
Size: 1645 4545
XmnI GAAnn'nnTTC
Cuts at: 2799 4319 2799
Size: 1520 4670
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3998 276
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI RsrII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI
PpuMI PspXI PsrI PvuII SacI SacII SanDI SexAI
SfiI SrfI SwaI XcmI