Restriction map for
pFN21AE4475
(Circular) (Six-base) MAPSORT of: pFN21AE4475.seq Check: 7510 from: 1 to: 6187
pFN21AE4475 6187 bp
With 182 enzymes: SgfI *
March 28, 2012 14:04 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3997 278
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3530 1937
Size: 1593 4594
Acc65I G'GTAC_C
Cuts at: 3508 3508
Size: 6187
AclI AA'CG_TT
Cuts at: 1563 2166 4314 4687 1563
Size: 603 2148 373 3063
Fragments arranged by size:
3063 2148 603 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3062 3306 4250 5634 5902 1632
Size: 1430 244 944 1384 268 1917
Fragments arranged by size:
1917 1430 1384 944 268 244
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5990
AflIII A'CryG_T
Cuts at: 1203 5086 6063 1203
Size: 3883 977 1327
Fragments arranged by size:
3883 1327 977
AgeI A'CCGG_T
Cuts at: 5018 5018
Size: 6187
AhdI GACnn_n'nnGTC
Cuts at: 4916 4916
Size: 6187
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6187
AlwNI CAG_nnn'CTG
Cuts at: 3061 3104 3930 5502 3061
Size: 43 826 1572 3746
Fragments arranged by size:
3746 1572 826 43
ApaLI G'TGCA_C
Cuts at: 4244 5400 4244
Size: 1156 5031
ApoI r'AATT_y
Cuts at: 1493 2528 3268 3496 3722 3874 5983 1493
Size: 1035 740 228 226 152 2109 1697
Fragments arranged by size:
2109 1697 1035 740 228 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2739 4741 160
Size: 872 1707 2002 1606
Fragments arranged by size:
2002 1707 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
AvaI C'yCGr_G
Cuts at: 1870 1943 3512 1870
Size: 73 1569 4545
Fragments arranged by size:
4545 1569 73
BamHI G'GATC_C
Cuts at: 2296 3517 3884 2296
Size: 1221 367 4599
Fragments arranged by size:
4599 1221 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3508 4963 618
Size: 325 217 627 1721 1455 1842
Fragments arranged by size:
1842 1721 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3506 1391
Size: 2115 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3035 928
Size: 971 1136 4080
Fragments arranged by size:
4080 1136 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4378 4412 1903
Size: 34 50 34 2357 34 3678
Fragments arranged by size:
3678 2357 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4079 5289 4079
Size: 1210 4977
BclI T'GATC_A
Cuts at: 1548 2506 2745 1548
Size: 958 239 4990
Fragments arranged by size:
4990 958 239
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4798 136
Size: 107 122 71 631 605 3126 1525
Fragments arranged by size:
3126 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6182 6182
Size: 6187
BlpI GC'TnA_GC
Cuts at: 3604 3604
Size: 6187
Bme1580I G_kGCm'C
Cuts at: 1790 3098 3117 4248 5404 1790
Size: 1308 19 1131 1156 2573
Fragments arranged by size:
2573 1308 1156 1131 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6187
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3918 4876 442
Size: 416 53 83 399 24 2501 958 1753
Fragments arranged by size:
2501 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6187
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3093 3093
Size: 6187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4847 1239
Size: 3608 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4312 5177 5475 4312
Size: 865 298 5024
Fragments arranged by size:
5024 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4850 882
Size: 617 3351 2219
Fragments arranged by size:
3351 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3379 3978 493
Size: 711 2175 599 2702
Fragments arranged by size:
2702 2175 711 599
BsaBI GATnn'nnATC
Cuts at: 6181 6181
Size: 6187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3994 4376 5011 275
Size: 53 83 186 936 2461 382 635 1451
Fragments arranged by size:
2461 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4620 5018 5292 5439 5850 4620
Size: 398 274 147 411 4957
Fragments arranged by size:
4957 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2434 2464 1178
Size: 30 165 30 1031 30 4901
Fragments arranged by size:
4901 1031 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 1186
Size: 934 5253
BseYI C'CCAG_C
Cuts at: 2208 2292 5390 5886 2208
Size: 84 3098 496 2509
Fragments arranged by size:
3098 2509 496 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5614
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3637 4398 5426 1540
Size: 275 186 1636 761 1028 2301
Fragments arranged by size:
2301 1636 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3279 4248 4333 5404 3279
Size: 969 85 1071 4062
Fragments arranged by size:
4062 1071 969 85
BsmI GAATG_Cn'
Cuts at: 3191 3698 3791 3191
Size: 507 93 5587
Fragments arranged by size:
5587 507 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3098 3117 3279 3506 4248 4333 5404
Size: 399 1308 19 162 227 742 85 1071
Cuts at: 5404 1391
Size: 2174
Fragments arranged by size:
2174 1308 1071 742 399 227 162 85
19
BspEI T'CCGG_A
Cuts at: 5850 5850
Size: 6187
BspHI T'CATG_A
Cuts at: 4076 5806 4076
Size: 1730 4457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2931 3542 844
Size: 2087 611 3489
Fragments arranged by size:
3489 2087 611
BsrBI CCG'CTC
Cuts at: 1394 1823 4074 1394
Size: 429 2251 3507
Fragments arranged by size:
3507 2251 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4682 4856 65
Size: 1183 3434 174 1396
Fragments arranged by size:
3434 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4831 5018 1700
Size: 110 3021 187 2869
Fragments arranged by size:
3021 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6187
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6187
BssSI C'ACGA_G
Cuts at: 4247 5259 4247
Size: 1012 5175
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3013 794
Size: 2219 3968
BstBI TT'CG_AA
Cuts at: 4991 4991
Size: 6187
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6187
BstXI CCAn_nnnn'nTGG
Cuts at: 2896 3255 3391 2896
Size: 359 136 5692
Fragments arranged by size:
5692 359 136
BstYI r'GATC_y
Cuts at: 1972 2296 3517 3884 4269 4286 5738 5824 5836
Size: 324 1221 367 385 17 1452 86 12
Cuts at: 5836 6182 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1221 385 367 346 324 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3160 3384 513
Size: 552 2095 224 3316
Fragments arranged by size:
3316 2095 552 224
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5448
Fragments arranged by size:
5448 683 56
BtsI GCAGTG_nn'
Cuts at: 3117 3703 4497 4517 3117
Size: 586 794 20 4787
Fragments arranged by size:
4787 794 586 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6152
DraI TTT'AAA
Cuts at: 2080 3491 2080
Size: 1411 4776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5194 809
Size: 507 3878 1802
Fragments arranged by size:
3878 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2214 3381
Size: 54 1425 50 275 18 369 15 1167
Cuts at: 3381 3634 4523 6152 8
Size: 253 889 1629 43
Fragments arranged by size:
1629 1425 1167 889 369 275 253 54
50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3634 1537
Size: 275 1822 4090
Fragments arranged by size:
4090 1822 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2120 2331 2424 2670 3329 4117 1353
Size: 767 211 93 246 659 788 3423
Fragments arranged by size:
3423 788 767 659 246 211 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4774 5148 5294 387
Size: 1064 138 3185 374 146 1280
Fragments arranged by size:
3185 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3062 3306 4250 4847 5634 5902 1239
Size: 393 1430 244 944 597 787 268 1524
Fragments arranged by size:
1524 1430 944 787 597 393 268 244
EcoNI CCTnn'n_nnAGG
Cuts at: 3141 3141
Size: 6187
EcoO109I rG'GnC_Cy
Cuts at: 3058 3058
Size: 6187
EcoRI G'AATT_C
Cuts at: 3496 3874 3496
Size: 378 5809
EcoRV GAT'ATC
Cuts at: 2488 2488
Size: 6187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2438 2840 3167 2438
Size: 402 327 5458
Fragments arranged by size:
5458 402 327
FspI TGC'GCA
Cuts at: 4693 4693
Size: 6187
HaeII r_GCGC'y
Cuts at: 1678 5334 1678
Size: 3656 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4828 4860 4902 4934 1373
Size: 32 135 32 3256 32 42 32 2626
Fragments arranged by size:
3256 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3531 3777 669
Size: 988 281 1593 246 3079
Fragments arranged by size:
3079 1593 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3149 748
Size: 2401 3786
HpaI GTT'AAC
Cuts at: 3777 3777
Size: 6187
KpnI G_GTAC'C
Cuts at: 3512 3512
Size: 6187
MfeI C'AATT_G
Cuts at: 3786 3786
Size: 6187
MluI A'CGCG_T
Cuts at: 6063 6063
Size: 6187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2196 5301 5485 1305
Size: 891 3105 184 2007
Fragments arranged by size:
3105 2007 891 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 2216 3383 10
Size: 54 1425 343 369 15 1167 2814
Fragments arranged by size:
2814 1425 1167 369 343 54 15
MslI CAynn'nnrTG
Cuts at: 518 1166 2960 3253 3389 4145 4504 4663 518
Size: 648 1794 293 136 756 359 159 2042
Fragments arranged by size:
2042 1794 756 648 359 293 159 136
MspA1I CmG'CkG
Cuts at: 1606 2212 3603 4280 5428 5673 1606
Size: 606 1391 677 1148 245 2120
Fragments arranged by size:
2120 1391 1148 677 606 245
NcoI C'CATG_G
Cuts at: 513 1065 3160 3384 513
Size: 552 2095 224 3316
Fragments arranged by size:
3316 2095 552 224
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6187
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6187
NotI GC'GGCC_GC
Cuts at: 3634 3634
Size: 6187
NspI r_CATG'y
Cuts at: 1132 1229 3545 5090 1132
Size: 97 2316 1545 2229
Fragments arranged by size:
2316 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6187
PciI A'CATG_T
Cuts at: 5086 5086
Size: 6187
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2242 2256 3467 1108
Size: 1134 14 1211 3828
Fragments arranged by size:
3828 1211 1134 14
PfoI T'CCnGG_A
Cuts at: 2537 2537
Size: 6187
PmeI GTTT'AAAC
Cuts at: 3491 3491
Size: 6187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4256 4288 5769 5801 4256
Size: 32 1481 32 4642
Fragments arranged by size:
4642 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3058 3058
Size: 6187
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6187
PsiI TTA'TAA
Cuts at: 2283 3419 3757 2283
Size: 1136 338 4713
Fragments arranged by size:
4713 1136 338
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2912 2944 2972 3004 2912
Size: 32 28 32 6095
Fragments arranged by size:
6095 32 32 28
PstI C_TGCA'G
Cuts at: 830 2341 3445 3539 830
Size: 1511 1104 94 3478
Fragments arranged by size:
3478 1511 1104 94
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6187
PvuII CAG'CTG
Cuts at: 2212 2212
Size: 6187
SalI G'TCGA_C
Cuts at: 1936 3529 1936
Size: 1593 4594
SapI GCTCTTCn'nnn_
Cuts at: 2120 2424 3329 2120
Size: 304 905 4978
Fragments arranged by size:
4978 905 304
SbfI CC_TGCA'GG
Cuts at: 3539 3539
Size: 6187
ScaI AGT'ACT
Cuts at: 4435 6147 4435
Size: 1712 4475
SfcI C'TryA_G
Cuts at: 826 1045 2337 3441 3455 3535 4670 5351 5542
Size: 219 1292 1104 14 80 1135 681 191
Cuts at: 5542 826
Size: 1471
Fragments arranged by size:
1471 1292 1135 1104 681 219 191 80
14
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6187
SmaI CCC'GGG
Cuts at: 1872 3514 1872
Size: 1642 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4291 5192 5454 820
Size: 197 926 2348 901 262 1553
Fragments arranged by size:
2348 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3978 493
Size: 3485 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6187
SphI G_CATG'C
Cuts at: 1132 3545 1132
Size: 2413 3774
SspI AAT'ATT
Cuts at: 5 52 4111 5
Size: 47 4059 2081
Fragments arranged by size:
4059 2081 47
StuI AGG'CCT
Cuts at: 1517 2155 3123 3136 1517
Size: 638 968 13 4568
Fragments arranged by size:
4568 968 638 13
StyI C'CwwG_G
Cuts at: 513 1065 3160 3351 3384 3626 513
Size: 552 2095 191 33 242 3074
Fragments arranged by size:
3074 2095 552 242 191 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2222 2903 3013 3247 4214 4231 4384
Size: 1255 642 681 110 234 967 17 153
Cuts at: 4384 4569 5014 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 967 681 642 445 234 185
153 110 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4433 6145 96
Size: 274 80 33 51 1443 2456 1712 138
Fragments arranged by size:
2456 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2571 2603 6147 6179 2571
Size: 32 3544 32 2579
Fragments arranged by size:
3544 2579 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2177 1316
Size: 861 5326
XbaI T'CTAG_A
Cuts at: 3523 3523
Size: 6187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2209 2896 2209
Size: 687 5500
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6187
XmaI C'CCGG_G
Cuts at: 1870 3512 1870
Size: 1642 4545
XmnI GAAnn'nnTTC
Cuts at: 4316 4316
Size: 6187
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3995 276
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI