Restriction map for
pFN21AE4474
(Circular) (Six-base) MAPSORT of: pFN21AE4474.seq Check: 5198 from: 1 to: 6184
pFN21AE4474 6184 bp
With 182 enzymes: SgfI *
February 29, 2012 12:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3243 3994 278
Size: 53 83 186 936 1707 751 2468
Fragments arranged by size:
2468 1707 936 751 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2523 3527 1937
Size: 586 1004 4594
Fragments arranged by size:
4594 1004 586
Acc65I G'GTAC_C
Cuts at: 3505 3505
Size: 6184
AclI AA'CG_TT
Cuts at: 1563 4311 4684 1563
Size: 2748 373 3063
Fragments arranged by size:
3063 2748 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2144 3434 3458 4247 5631 5899 1632
Size: 512 1290 24 789 1384 268 1917
Fragments arranged by size:
1917 1384 1290 789 512 268 24
AfeI AGC'GCT
Cuts at: 2456 2558 2456
Size: 102 6082
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5987
AflIII A'CryG_T
Cuts at: 1203 2106 2820 5083 6060 1203
Size: 903 714 2263 977 1327
Fragments arranged by size:
2263 1327 977 903 714
AgeI A'CCGG_T
Cuts at: 5015 5015
Size: 6184
AhdI GACnn_n'nnGTC
Cuts at: 2359 4913 2359
Size: 2554 3630
AleI CACnn'nnGTG
Cuts at: 1166 2744 3347 1166
Size: 1578 603 4003
Fragments arranged by size:
4003 1578 603
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6184
AlwNI CAG_nnn'CTG
Cuts at: 3927 5499 3927
Size: 1572 4612
ApaI G_GGCC'C
Cuts at: 2712 2712
Size: 6184
ApaLI G'TGCA_C
Cuts at: 2405 4241 5397 2405
Size: 1836 1156 3192
Fragments arranged by size:
3192 1836 1156
ApoI r'AATT_y
Cuts at: 1493 3493 3719 3871 5980 1493
Size: 2000 226 152 2109 1697
Fragments arranged by size:
2109 2000 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2615 2615
Size: 6184
AseI AT'TA_AT
Cuts at: 160 1032 4738 160
Size: 872 3706 1606
Fragments arranged by size:
3706 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
AvaI C'yCGr_G
Cuts at: 1870 1943 2621 3509 1870
Size: 73 678 888 4545
Fragments arranged by size:
4545 888 678 73
BamHI G'GATC_C
Cuts at: 3514 3881 3514
Size: 367 5817
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2254 2345 2604 2698 3209
Size: 325 217 627 467 91 259 94 511
Cuts at: 3209 3266 3505 4960 618
Size: 57 239 1455 1842
Fragments arranged by size:
1842 1455 627 511 467 325 259 239
217 94 91 57
BanII G_rGCy'C
Cuts at: 1391 2712 2763 3129 3503 1391
Size: 1321 51 366 374 4072
Fragments arranged by size:
4072 1321 374 366 51
BbeI G_GCGC'C
Cuts at: 2608 2702 3270 2608
Size: 94 568 5522
Fragments arranged by size:
5522 568 94
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3235 928
Size: 971 1336 3877
Fragments arranged by size:
3877 1336 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2747 2781 4375 4409 1903
Size: 34 810 34 1594 34 3678
Fragments arranged by size:
3678 1594 810 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4076 5286 4076
Size: 1210 4974
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6184
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2202 2707 4795
Size: 107 122 71 631 605 530 505 2088
Cuts at: 4795 136
Size: 1525
Fragments arranged by size:
2088 1525 631 605 530 505 122 107
71
BglII A'GATC_T
Cuts at: 2136 6179 2136
Size: 4043 2141
BlpI GC'TnA_GC
Cuts at: 3601 3601
Size: 6184
Bme1580I G_kGCm'C
Cuts at: 1790 2259 2409 2514 2712 3212 4245 5401 1790
Size: 469 150 105 198 500 1033 1156 2573
Fragments arranged by size:
2573 1156 1033 500 469 198 150 105
BmgBI CAC'GTC
Cuts at: 1318 2509 1318
Size: 1191 4993
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3915 4873 442
Size: 416 53 83 399 24 2498 958 1753
Fragments arranged by size:
2498 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6184
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 6184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 2666 2792 2912 2971 3214 3272 4844
Size: 929 498 126 120 59 243 58 1572
Cuts at: 4844 1239
Size: 2579
Fragments arranged by size:
2579 1572 929 498 243 126 120 59
58
Bpu10I CC'TnA_GC
Cuts at: 2468 2468
Size: 6184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2708 4309 5174 5472 2708
Size: 1601 865 298 3420
Fragments arranged by size:
3420 1601 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2861 2873 4847 882
Size: 617 1362 12 1974 2219
Fragments arranged by size:
2219 1974 1362 617 12
BsaAI yAC'GTr
Cuts at: 493 1204 3975 493
Size: 711 2771 2702
Fragments arranged by size:
2771 2702 711
BsaBI GATnn'nnATC
Cuts at: 6178 6178
Size: 6184
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2520 2605 2699 3050
Size: 53 83 186 936 987 85 94 351
Cuts at: 3050 3219 3240 3267 3432 3991 4373 5008 275
Size: 169 21 27 165 559 382 635 1451
Fragments arranged by size:
1451 987 936 635 559 382 351 186
169 165 94 85 83 53 27 21
BsaWI w'CCGG_w
Cuts at: 4617 5015 5289 5436 5847 4617
Size: 398 274 147 411 4954
Fragments arranged by size:
4954 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5959
Fragments arranged by size:
5959 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2879 1186
Size: 1693 4491
BseYI C'CCAG_C
Cuts at: 2711 5387 5883 2711
Size: 2676 496 3012
Fragments arranged by size:
3012 2676 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2054 2588 2993 1163
Size: 573 318 534 405 4354
Fragments arranged by size:
4354 573 534 405 318
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2082 2181 2302 2480 2484 2902
Size: 275 186 81 99 121 178 4 418
Cuts at: 2902 3634 4395 5423 1540
Size: 732 761 1028 2301
Fragments arranged by size:
2301 1028 761 732 418 275 186 178
121 99 81 4
BsiHKAI G_wGCw'C
Cuts at: 2409 2763 3129 4245 4330 5401 2409
Size: 354 366 1116 85 1071 3192
Fragments arranged by size:
3192 1116 1071 366 354 85
BsmI GAATG_Cn'
Cuts at: 3001 3695 3788 3001
Size: 694 93 5397
Fragments arranged by size:
5397 694 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2259 2409 2514 2712 2763 3129 3212
Size: 399 469 150 105 198 51 366 83
Cuts at: 3212 3503 4245 4330 5401 1391
Size: 291 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 469 399 366 291 198
150 105 85 83 51
BspEI T'CCGG_A
Cuts at: 5847 5847
Size: 6184
BspHI T'CATG_A
Cuts at: 3135 4073 5803 3135
Size: 938 1730 3516
Fragments arranged by size:
3516 1730 938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2249 3539 844
Size: 1405 1290 3489
Fragments arranged by size:
3489 1405 1290
BsrBI CCG'CTC
Cuts at: 1394 1823 2655 3228 4071 1394
Size: 429 832 573 843 3507
Fragments arranged by size:
3507 843 832 573 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4679 4853 65
Size: 1183 3431 174 1396
Fragments arranged by size:
3431 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2328 2479 2626 3030 4828 5015 1700
Size: 110 518 151 147 404 1798 187 2869
Fragments arranged by size:
2869 1798 518 404 187 151 147 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6184
BssHII G'CGCG_C
Cuts at: 1925 2472 2615 1925
Size: 547 143 5494
Fragments arranged by size:
5494 547 143
BssSI C'ACGA_G
Cuts at: 4244 5256 4244
Size: 1012 5172
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6184
BstBI TT'CG_AA
Cuts at: 4988 4988
Size: 6184
BstEII G'GTnAC_C
Cuts at: 1184 3325 1184
Size: 2141 4043
BstXI CCAn_nnnn'nTGG
Cuts at: 2746 3397 2746
Size: 651 5533
BstYI r'GATC_y
Cuts at: 1972 2037 2136 3514 3881 4266 4283 5735 5821
Size: 65 99 1378 367 385 17 1452 86
Cuts at: 5821 5833 6179 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1378 385 367 346 99 86
65 17 12
Bsu36I CC'TnA_GG
Cuts at: 2424 2424
Size: 6184
BtgI C'CryG_G
Cuts at: 513 1065 2496 3169 3321 3456 513
Size: 552 1431 673 152 135 3241
Fragments arranged by size:
3241 1431 673 552 152 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2395 487
Size: 683 56 1169 4276
Fragments arranged by size:
4276 1169 683 56
BtsI GCAGTG_nn'
Cuts at: 3319 3700 4494 4514 3319
Size: 381 794 20 4989
Fragments arranged by size:
4989 794 381 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6149
DraI TTT'AAA
Cuts at: 3488 3488
Size: 6184
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3323 5191 809
Size: 507 2007 1868 1802
Fragments arranged by size:
2007 1868 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2178 2477
Size: 54 1425 50 275 18 249 99 299
Cuts at: 2477 2481 2628 2805 2958 3093 3387 3631 4520
Size: 4 147 177 153 135 294 244 889
Cuts at: 4520 6149 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 299 294 275 249 244
177 153 147 135 99 54 50 43
18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2079 2178 2477 2481 3631 1537
Size: 275 267 99 299 4 1150 4090
Fragments arranged by size:
4090 1150 299 275 267 99 4
EarI CTCTTCn'nnn_
Cuts at: 1353 3134 4114 1353
Size: 1781 980 3423
Fragments arranged by size:
3423 1781 980
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2101 2234 3464 4771 5145 5291
Size: 1064 138 512 133 1230 1307 374 146
Cuts at: 5291 387
Size: 1280
Fragments arranged by size:
1307 1280 1230 1064 512 374 146 138
133
EcoICRI GAG'CTC
Cuts at: 2761 3127 2761
Size: 366 5818
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2168 2666 2792 2912 2971 3214
Size: 393 512 24 498 126 120 59 243
Cuts at: 3214 3272 3434 3458 4247 4844 5631 5899 1239
Size: 58 162 24 789 597 787 268 1524
Fragments arranged by size:
1524 789 787 597 512 498 393 268
243 162 126 120 59 58 24 24
EcoO109I rG'GnC_Cy
Cuts at: 2708 2708
Size: 6184
EcoRI G'AATT_C
Cuts at: 3493 3871 3493
Size: 378 5806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2686 2938 2686
Size: 252 5932
FseI GG_CCGG'CC
Cuts at: 2483 2483
Size: 6184
FspI TGC'GCA
Cuts at: 2192 4690 2192
Size: 2498 3686
HaeII r_GCGC'y
Cuts at: 1678 2335 2458 2560 2608 2702 3270 3409 5331
Size: 657 123 102 48 94 568 139 1922
Cuts at: 5331 1678
Size: 2531
Fragments arranged by size:
2531 1922 657 568 139 123 102 94
48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 3452 3484 4825
Size: 32 135 32 773 32 1075 32 1341
Cuts at: 4825 4857 4899 4931 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1341 1075 773 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2431 2524 3528 3774 669
Size: 988 281 493 93 1004 246 3079
Fragments arranged by size:
3079 1004 988 493 281 246 93
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6184
HpaI GTT'AAC
Cuts at: 3774 3774
Size: 6184
KasI G'GCGC_C
Cuts at: 2604 2698 3266 2604
Size: 94 568 5522
Fragments arranged by size:
5522 568 94
KpnI G_GTAC'C
Cuts at: 3509 3509
Size: 6184
MfeI C'AATT_G
Cuts at: 3783 3783
Size: 6184
MluI A'CGCG_T
Cuts at: 2106 6060 2106
Size: 3954 2230
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5298 5482 1305
Size: 3993 184 2007
Fragments arranged by size:
3993 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3389 10
Size: 54 1425 343 1557 2805
Fragments arranged by size:
2805 1557 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2414 2744 3161 3347 3395 4142 4501
Size: 648 1248 330 417 186 48 747 359
Cuts at: 4501 4660 518
Size: 159 2042
Fragments arranged by size:
2042 1248 747 648 417 359 330 186
159 48
MspA1I CmG'CkG
Cuts at: 1606 2715 2890 3003 3091 3171 3250 3323 3336
Size: 1109 175 113 88 80 79 73 13
Cuts at: 3336 3600 4277 5425 5670 1606
Size: 264 677 1148 245 2120
Fragments arranged by size:
2120 1148 1109 677 264 245 175 113
88 80 79 73 13
NaeI GCC'GGC
Cuts at: 2481 2628 2481
Size: 147 6037
NarI GG'CG_CC
Cuts at: 2605 2699 3267 2605
Size: 94 568 5522
Fragments arranged by size:
5522 568 94
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5632
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6184
NgoMIV G'CCGG_C
Cuts at: 2479 2626 2479
Size: 147 6037
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6184
NotI GC'GGCC_GC
Cuts at: 3631 3631
Size: 6184
NspI r_CATG'y
Cuts at: 1132 1229 3542 5087 1132
Size: 97 2313 1545 2229
Fragments arranged by size:
2313 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2514 1348
Size: 1166 5018
PciI A'CATG_T
Cuts at: 5083 5083
Size: 6184
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2839 1108
Size: 1731 4453
PfoI T'CCnGG_A
Cuts at: 2547 2547
Size: 6184
PmeI GTTT'AAAC
Cuts at: 3488 3488
Size: 6184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4253 4285 5766 5798 4253
Size: 32 1481 32 4639
Fragments arranged by size:
4639 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2298 1613
Size: 685 5499
PsiI TTA'TAA
Cuts at: 3754 3754
Size: 6184
PspOMI G'GGCC_C
Cuts at: 2708 2708
Size: 6184
PstI C_TGCA'G
Cuts at: 830 2316 2802 3536 830
Size: 1486 486 734 3478
Fragments arranged by size:
3478 1486 734 486
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6184
PvuII CAG'CTG
Cuts at: 2890 3003 3091 3250 2890
Size: 113 88 159 5824
Fragments arranged by size:
5824 159 113 88
SacI G_AGCT'C
Cuts at: 2763 3129 2763
Size: 366 5818
SacII CC_GC'GG
Cuts at: 3172 3324 3172
Size: 152 6032
SalI G'TCGA_C
Cuts at: 1936 2522 3526 1936
Size: 586 1004 4594
Fragments arranged by size:
4594 1004 586
SapI GCTCTTCn'nnn_
Cuts at: 3134 3134
Size: 6184
SbfI CC_TGCA'GG
Cuts at: 3536 3536
Size: 6184
ScaI AGT'ACT
Cuts at: 2945 4432 6144 2945
Size: 1487 1712 2985
Fragments arranged by size:
2985 1712 1487
SfcI C'TryA_G
Cuts at: 826 1045 2312 2437 2560 2798 3532 4667 5348
Size: 219 1267 125 123 238 734 1135 681
Cuts at: 5348 5539 826
Size: 191 1471
Fragments arranged by size:
1471 1267 1135 734 681 238 219 191
125 123
SfoI GGC'GCC
Cuts at: 2606 2700 3268 2606
Size: 94 568 5522
Fragments arranged by size:
5522 568 94
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
SgrAI Cr'CCGG_yG
Cuts at: 1700 2328 1700
Size: 628 5556
SmaI CCC'GGG
Cuts at: 1872 3511 1872
Size: 1639 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2723 4288 5189 5451 820
Size: 197 926 780 1565 901 262 1553
Fragments arranged by size:
1565 1553 926 901 780 262 197
SnaBI TAC'GTA
Cuts at: 493 3975 493
Size: 3482 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6184
SphI G_CATG'C
Cuts at: 1132 3542 1132
Size: 2410 3774
SspI AAT'ATT
Cuts at: 5 52 4108 5
Size: 47 4056 2081
Fragments arranged by size:
4056 2081 47
StuI AGG'CCT
Cuts at: 1517 3443 1517
Size: 1926 4258
StyI C'CwwG_G
Cuts at: 513 1065 2742 3623 513
Size: 552 1677 881 3074
Fragments arranged by size:
3074 1677 881 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2885 4211 4228 4381 4566 5011 325
Size: 1255 1305 1326 17 153 185 445 1498
Fragments arranged by size:
1498 1326 1305 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2943 4430 6142
Size: 274 80 33 51 1443 966 1487 1712
Cuts at: 6142 96
Size: 138
Fragments arranged by size:
1712 1487 1443 966 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6144 6176 6144
Size: 32 6152
Tth111I GACn'n_nGTC
Cuts at: 1316 2380 1316
Size: 1064 5120
XbaI T'CTAG_A
Cuts at: 3520 3520
Size: 6184
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6184
XmaI C'CCGG_G
Cuts at: 1870 3509 1870
Size: 1639 4545
XmnI GAAnn'nnTTC
Cuts at: 4313 4313
Size: 6184
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3241 3992 276
Size: 53 83 186 936 1707 751 2468
Fragments arranged by size:
2468 1707 936 751 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AvrII BaeI BbvCI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoNI EcoRV FspAI NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SanDI SexAI SfiI
SrfI SwaI XcmI