Restriction map for
pFN21AE4472
(Circular) (Six-base) MAPSORT of: pFN21AE4472.seq Check: 9557 from: 1 to: 6184
pFN21AE4472 6184 bp
With 182 enzymes: SgfI *
March 28, 2012 13:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3462 3462
Size: 6184
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3994 278
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3527 1937
Size: 1590 4594
Acc65I G'GTAC_C
Cuts at: 3505 3505
Size: 6184
AclI AA'CG_TT
Cuts at: 1563 4311 4684 1563
Size: 2748 373 3063
Fragments arranged by size:
3063 2748 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2366 2600 2612 3066 4247 5631 5899 1632
Size: 734 234 12 454 1181 1384 268 1917
Fragments arranged by size:
1917 1384 1181 734 454 268 234 12
AflII C'TTAA_G
Cuts at: 820 1017 3199 820
Size: 197 2182 3805
Fragments arranged by size:
3805 2182 197
AflIII A'CryG_T
Cuts at: 1203 2319 5083 6060 1203
Size: 1116 2764 977 1327
Fragments arranged by size:
2764 1327 1116 977
AgeI A'CCGG_T
Cuts at: 5015 5015
Size: 6184
AhdI GACnn_n'nnGTC
Cuts at: 4913 4913
Size: 6184
AleI CACnn'nnGTG
Cuts at: 1166 3299 1166
Size: 2133 4051
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6184
AlwNI CAG_nnn'CTG
Cuts at: 3927 5499 3927
Size: 1572 4612
ApaLI G'TGCA_C
Cuts at: 4241 5397 4241
Size: 1156 5028
ApoI r'AATT_y
Cuts at: 1493 2560 3095 3493 3719 3871 5980 1493
Size: 1067 535 398 226 152 2109 1697
Fragments arranged by size:
2109 1697 1067 535 398 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4738 160
Size: 872 3706 1606
Fragments arranged by size:
3706 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
AvaI C'yCGr_G
Cuts at: 1870 1943 3329 3405 3509 1870
Size: 73 1386 76 104 4545
Fragments arranged by size:
4545 1386 104 76 73
BamHI G'GATC_C
Cuts at: 3514 3881 3514
Size: 367 5817
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 3505 4960 618
Size: 325 217 627 279 1439 1455 1842
Fragments arranged by size:
1842 1455 1439 627 325 279 217
BanII G_rGCy'C
Cuts at: 1391 3503 1391
Size: 2112 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2323 2854 928
Size: 971 424 531 4258
Fragments arranged by size:
4258 971 531 424
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4375 4409 1903
Size: 34 50 34 2354 34 3678
Fragments arranged by size:
3678 2354 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4076 5286 4076
Size: 1210 4974
BclI T'GATC_A
Cuts at: 1548 2383 3291 1548
Size: 835 908 4441
Fragments arranged by size:
4441 908 835
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4795 136
Size: 107 122 71 631 605 337 2786 1525
Fragments arranged by size:
2786 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2601 3045 6179 2601
Size: 444 3134 2606
Fragments arranged by size:
3134 2606 444
BlpI GC'TnA_GC
Cuts at: 2909 3601 2909
Size: 692 5492
Bme1580I G_kGCm'C
Cuts at: 1790 4245 5401 1790
Size: 2455 1156 2573
Fragments arranged by size:
2573 2455 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6184
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3915 4873 442
Size: 416 53 83 399 24 2498 958 1753
Fragments arranged by size:
2498 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6184
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2425 3274 2425
Size: 849 5335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2408 2759 2963 3455 4844 1239
Size: 1169 351 204 492 1389 2579
Fragments arranged by size:
2579 1389 1169 492 351 204
Bpu10I CC'TnA_GC
Cuts at: 3447 3447
Size: 6184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3017 3499 4309 5174 5472 3017
Size: 482 810 865 298 3729
Fragments arranged by size:
3729 865 810 482 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2939 4847 882
Size: 617 1440 1908 2219
Fragments arranged by size:
2219 1908 1440 617
BsaAI yAC'GTr
Cuts at: 493 1204 3975 493
Size: 711 2771 2702
Fragments arranged by size:
2771 2702 711
BsaBI GATnn'nnATC
Cuts at: 6178 6178
Size: 6184
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3991 4373 5008 275
Size: 53 83 186 936 2458 382 635 1451
Fragments arranged by size:
2458 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4617 5015 5289 5436 5847 4617
Size: 398 274 147 411 4954
Fragments arranged by size:
4954 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3057 3087 1178
Size: 30 165 30 1654 30 4275
Fragments arranged by size:
4275 1654 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6184
BseYI C'CCAG_C
Cuts at: 2038 2374 2818 3002 5387 5883 2038
Size: 336 444 184 2385 496 2339
Fragments arranged by size:
2385 2339 496 444 336 184
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5611
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3634 4395 5423 1540
Size: 275 186 1633 761 1028 2301
Fragments arranged by size:
2301 1633 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3453 4245 4330 5401 3453
Size: 792 85 1071 4236
Fragments arranged by size:
4236 1071 792 85
BsmI GAATG_Cn'
Cuts at: 3695 3788 3695
Size: 93 6091
BsmBI CGTCTCn'nnnn_
Cuts at: 2916 2916
Size: 6184
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3453 3503 4245 4330 5401 1391
Size: 399 1663 50 742 85 1071 2174
Fragments arranged by size:
2174 1663 1071 742 399 85 50
BspEI T'CCGG_A
Cuts at: 5847 5847
Size: 6184
BspHI T'CATG_A
Cuts at: 3351 4073 5803 3351
Size: 722 1730 3732
Fragments arranged by size:
3732 1730 722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3462 3539 844
Size: 2618 77 3489
Fragments arranged by size:
3489 2618 77
BsrBI CCG'CTC
Cuts at: 1394 1823 4071 1394
Size: 429 2248 3507
Fragments arranged by size:
3507 2248 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2050 4679 4853 65
Size: 1183 802 2629 174 1396
Fragments arranged by size:
2629 1396 1183 802 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4828 5015 1700
Size: 110 3018 187 2869
Fragments arranged by size:
3018 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6184
BssSI C'ACGA_G
Cuts at: 2075 2129 4244 5256 2075
Size: 54 2115 1012 3003
Fragments arranged by size:
3003 2115 1012 54
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2091 794
Size: 1297 4887
BstBI TT'CG_AA
Cuts at: 4988 4988
Size: 6184
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6184
BstXI CCAn_nnnn'nTGG
Cuts at: 2818 2818
Size: 6184
BstYI r'GATC_y
Cuts at: 1972 2601 3045 3514 3881 4266 4283 5735 5821
Size: 629 444 469 367 385 17 1452 86
Cuts at: 5821 5833 6179 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 629 469 444 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3312 3312
Size: 6184
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5632
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2245 487
Size: 683 56 1019 4426
Fragments arranged by size:
4426 1019 683 56
BtsI GCAGTG_nn'
Cuts at: 2361 2813 2943 3025 3700 4494 4514 2361
Size: 452 130 82 675 794 20 4031
Fragments arranged by size:
4031 794 675 452 130 82 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6149
DraI TTT'AAA
Cuts at: 3488 3488
Size: 6184
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5191 809
Size: 507 3875 1802
Fragments arranged by size:
3875 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2212 2637 2874
Size: 54 1425 50 275 18 382 425 237
Cuts at: 2874 2896 3631 4520 6149 8
Size: 22 735 889 1629 43
Fragments arranged by size:
1629 1425 889 735 425 382 275 237
54 50 43 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 3631 1537
Size: 275 1819 4090
Fragments arranged by size:
4090 1819 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3141 3200 3380 4114 1353
Size: 1788 59 180 734 3423
Fragments arranged by size:
3423 1788 734 180 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4771 5145 5291 387
Size: 1064 138 3182 374 146 1280
Fragments arranged by size:
3182 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2366 2408 2600 2612 2759 2963 3066
Size: 393 734 42 192 12 147 204 103
Cuts at: 3066 3455 4247 4844 5631 5899 1239
Size: 389 792 597 787 268 1524
Fragments arranged by size:
1524 792 787 734 597 393 389 268
204 192 147 103 42 12
EcoO109I rG'GnC_Cy
Cuts at: 2340 2785 2340
Size: 445 5739
EcoRI G'AATT_C
Cuts at: 3493 3871 3493
Size: 378 5806
EcoRV GAT'ATC
Cuts at: 2182 3154 2182
Size: 972 5212
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3214 3214
Size: 6184
FspI TGC'GCA
Cuts at: 4690 4690
Size: 6184
HaeII r_GCGC'y
Cuts at: 1678 5331 1678
Size: 3653 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2698 2730 3057 3089 3305
Size: 32 135 32 1126 32 327 32 216
Cuts at: 3305 3337 3388 3420 4825 4857 4899 4931 1373
Size: 32 51 32 1405 32 42 32 2626
Fragments arranged by size:
2626 1405 1126 327 216 135 51 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3528 3774 669
Size: 988 281 1590 246 3079
Fragments arranged by size:
3079 1590 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6184
HpaI GTT'AAC
Cuts at: 3774 3774
Size: 6184
KpnI G_GTAC'C
Cuts at: 3509 3509
Size: 6184
MfeI C'AATT_G
Cuts at: 3783 3783
Size: 6184
MluI A'CGCG_T
Cuts at: 6060 6060
Size: 6184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3264 5298 5482 1305
Size: 1959 2034 184 2007
Fragments arranged by size:
2034 2007 1959 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2214 2876 10
Size: 54 1425 343 382 662 3318
Fragments arranged by size:
3318 1425 662 382 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2690 3299 4142 4501 4660 518
Size: 648 1524 609 843 359 159 2042
Fragments arranged by size:
2042 1524 843 648 609 359 159
MspA1I CmG'CkG
Cuts at: 1606 2440 2551 3299 3600 4277 5425 5670 1606
Size: 834 111 748 301 677 1148 245 2120
Fragments arranged by size:
2120 1148 834 748 677 301 245 111
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5632
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6184
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6184
NotI GC'GGCC_GC
Cuts at: 3631 3631
Size: 6184
NspI r_CATG'y
Cuts at: 1132 1229 2836 3542 5087 1132
Size: 97 1607 706 1545 2229
Fragments arranged by size:
2229 1607 1545 706 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6184
PciI A'CATG_T
Cuts at: 5083 5083
Size: 6184
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2367 2872 1108
Size: 1259 505 4420
Fragments arranged by size:
4420 1259 505
PfoI T'CCnGG_A
Cuts at: 2889 2889
Size: 6184
PmeI GTTT'AAAC
Cuts at: 3488 3488
Size: 6184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4253 4285 5766 5798 4253
Size: 32 1481 32 4639
Fragments arranged by size:
4639 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2340 2785 2340
Size: 445 5739
PshAI GACnn'nnGTC
Cuts at: 1613 3164 1613
Size: 1551 4633
PsiI TTA'TAA
Cuts at: 3754 3754
Size: 6184
PspXI vC'TCGA_Gb
Cuts at: 3329 3329
Size: 6184
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2831 2863 2831
Size: 32 6152
PstI C_TGCA'G
Cuts at: 830 3285 3429 3536 830
Size: 2455 144 107 3478
Fragments arranged by size:
3478 2455 144 107
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6184
PvuII CAG'CTG
Cuts at: 2440 2551 2440
Size: 111 6073
RsrII CG'GwC_CG
Cuts at: 2466 2466
Size: 6184
SalI G'TCGA_C
Cuts at: 1936 3526 1936
Size: 1590 4594
SanDI GG'GwC_CC
Cuts at: 2340 2340
Size: 6184
SapI GCTCTTCn'nnn_
Cuts at: 3200 3200
Size: 6184
SbfI CC_TGCA'GG
Cuts at: 3536 3536
Size: 6184
ScaI AGT'ACT
Cuts at: 4432 6144 4432
Size: 1712 4472
SfcI C'TryA_G
Cuts at: 826 1045 3129 3281 3425 3532 4667 5348 5539
Size: 219 2084 152 144 107 1135 681 191
Cuts at: 5539 826
Size: 1471
Fragments arranged by size:
2084 1471 1135 681 219 191 152 144
107
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6184
SmaI CCC'GGG
Cuts at: 1872 3511 1872
Size: 1639 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3032 3199 3329 3405 3478 4288
Size: 197 926 1089 167 130 76 73 810
Cuts at: 4288 5189 5451 820
Size: 901 262 1553
Fragments arranged by size:
1553 1089 926 901 810 262 197 167
130 76 73
SnaBI TAC'GTA
Cuts at: 493 3975 493
Size: 3482 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6184
SphI G_CATG'C
Cuts at: 1132 2836 3542 1132
Size: 1704 706 3774
Fragments arranged by size:
3774 1704 706
SspI AAT'ATT
Cuts at: 5 52 4108 5
Size: 47 4056 2081
Fragments arranged by size:
4056 2081 47
StuI AGG'CCT
Cuts at: 1517 3173 1517
Size: 1656 4528
StyI C'CwwG_G
Cuts at: 513 1065 2139 2209 2518 2877 3623 513
Size: 552 1074 70 309 359 746 3074
Fragments arranged by size:
3074 1074 746 552 359 309 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2483 2526 4211 4228 4381 4566 5011
Size: 1255 903 43 1685 17 153 185 445
Cuts at: 5011 325
Size: 1498
Fragments arranged by size:
1685 1498 1255 903 445 185 153 43
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2838 4430 6142
Size: 274 80 33 51 1443 861 1592 1712
Cuts at: 6142 96
Size: 138
Fragments arranged by size:
1712 1592 1443 861 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6144 6176 6144
Size: 32 6152
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6184
XbaI T'CTAG_A
Cuts at: 3520 3520
Size: 6184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2222 2272 2222
Size: 50 6134
XhoI C'TCGA_G
Cuts at: 1943 3329 3405 1943
Size: 1386 76 4722
Fragments arranged by size:
4722 1386 76
XmaI C'CCGG_G
Cuts at: 1870 3509 1870
Size: 1639 4545
XmnI GAAnn'nnTTC
Cuts at: 4313 4313
Size: 6184
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3992 276
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I DraIII EcoICRI EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI SacI SacII SexAI SfiI SfoI SrfI SwaI