Restriction map for
pFN21AE4470
(Circular) (Six-base) MAPSORT of: pFN21AE4470.seq Check: 7010 from: 1 to: 6181
pFN21AE4470 6181 bp
With 182 enzymes: SgfI *
March 28, 2012 13:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3991 278
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2112 3524 1937
Size: 175 1412 4594
Fragments arranged by size:
4594 1412 175
Acc65I G'GTAC_C
Cuts at: 2375 3502 2375
Size: 1127 5054
AclI AA'CG_TT
Cuts at: 1563 4308 4681 1563
Size: 2745 373 3063
Fragments arranged by size:
3063 2745 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2462 4244 5628 5896 1632
Size: 830 1782 1384 268 1917
Fragments arranged by size:
1917 1782 1384 830 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5984
AflIII A'CryG_T
Cuts at: 1203 2841 5080 6057 1203
Size: 1638 2239 977 1327
Fragments arranged by size:
2239 1638 1327 977
AgeI A'CCGG_T
Cuts at: 5012 5012
Size: 6181
AhdI GACnn_n'nnGTC
Cuts at: 4910 4910
Size: 6181
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3137 1184
Size: 1953 4228
AlwNI CAG_nnn'CTG
Cuts at: 3924 5496 3924
Size: 1572 4609
ApaLI G'TGCA_C
Cuts at: 2233 4238 5394 2233
Size: 2005 1156 3020
Fragments arranged by size:
3020 2005 1156
ApoI r'AATT_y
Cuts at: 1493 3490 3716 3868 5977 1493
Size: 1997 226 152 2109 1697
Fragments arranged by size:
2109 1997 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4735 160
Size: 872 3703 1606
Fragments arranged by size:
3703 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
AvaI C'yCGr_G
Cuts at: 1870 1943 2164 2794 2867 3506 1870
Size: 73 221 630 73 639 4545
Fragments arranged by size:
4545 639 630 221 73 73
BamHI G'GATC_C
Cuts at: 2618 3511 3878 2618
Size: 893 367 4921
Fragments arranged by size:
4921 893 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2375 2508 3502 4957 618
Size: 325 217 627 588 133 994 1455 1842
Fragments arranged by size:
1842 1455 994 627 588 325 217 133
BanII G_rGCy'C
Cuts at: 1391 3500 1391
Size: 2109 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3185 928
Size: 971 1286 3924
Fragments arranged by size:
3924 1286 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4372 4406 1903
Size: 34 2435 34 3678
Fragments arranged by size:
3678 2435 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2054 2972 3370 4073 5283 2054
Size: 918 398 703 1210 2952
Fragments arranged by size:
2952 1210 918 703 398
BclI T'GATC_A
Cuts at: 1548 3261 1548
Size: 1713 4468
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4792 136
Size: 107 122 71 631 605 3120 1525
Fragments arranged by size:
3120 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6176 6176
Size: 6181
BlpI GC'TnA_GC
Cuts at: 3598 3598
Size: 6181
Bme1580I G_kGCm'C
Cuts at: 1790 2154 2237 4242 5398 1790
Size: 364 83 2005 1156 2573
Fragments arranged by size:
2573 2005 1156 364 83
BmgBI CAC'GTC
Cuts at: 1318 2827 1318
Size: 1509 4672
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2149 3912 4870
Size: 416 53 83 399 24 732 1763 958
Cuts at: 4870 442
Size: 1753
Fragments arranged by size:
1763 1753 958 732 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6181
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 6181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4841 1239
Size: 3602 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2737 4306 5171 5469 2737
Size: 1569 865 298 3449
Fragments arranged by size:
3449 1569 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4844 882
Size: 617 3345 2219
Fragments arranged by size:
3345 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2842 3972 493
Size: 711 1638 1130 2702
Fragments arranged by size:
2702 1638 1130 711
BsaBI GATnn'nnATC
Cuts at: 6175 6175
Size: 6181
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3988 4370 5005 275
Size: 53 83 186 936 2455 382 635 1451
Fragments arranged by size:
2455 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2068 4614 5012 5286 5433 5844 2068
Size: 2546 398 274 147 411 2405
Fragments arranged by size:
2546 2405 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5956
Fragments arranged by size:
5956 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2359 3182 1186
Size: 1173 823 4185
Fragments arranged by size:
4185 1173 823
BseYI C'CCAG_C
Cuts at: 3384 5384 5880 3384
Size: 2000 496 3685
Fragments arranged by size:
3685 2000 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2185 2807 1163
Size: 573 449 622 4537
Fragments arranged by size:
4537 622 573 449
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3631 4392 5420 1540
Size: 275 186 1630 761 1028 2301
Fragments arranged by size:
2301 1630 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2237 2345 4242 4327 5398 2237
Size: 108 1897 85 1071 3020
Fragments arranged by size:
3020 1897 1071 108 85
BsmI GAATG_Cn'
Cuts at: 3692 3785 3692
Size: 93 6088
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2237 2345 3500 4242 4327 5398
Size: 399 364 83 108 1155 742 85 1071
Cuts at: 5398 1391
Size: 2174
Fragments arranged by size:
2174 1155 1071 742 399 364 108 85
83
BspEI T'CCGG_A
Cuts at: 5844 5844
Size: 6181
BspHI T'CATG_A
Cuts at: 4070 5800 4070
Size: 1730 4451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2387 2803 3536 844
Size: 1543 416 733 3489
Fragments arranged by size:
3489 1543 733 416
BsrBI CCG'CTC
Cuts at: 1394 1823 4068 1394
Size: 429 2245 3507
Fragments arranged by size:
3507 2245 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2662 3039 4676 4850 65
Size: 1183 1414 377 1637 174 1396
Fragments arranged by size:
1637 1414 1396 1183 377 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2651 3284 4825 5012 1700
Size: 110 841 633 1541 187 2869
Fragments arranged by size:
2869 1541 841 633 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6181
BssHII G'CGCG_C
Cuts at: 1925 2787 1925
Size: 862 5319
BssSI C'ACGA_G
Cuts at: 2288 4241 5253 2288
Size: 1953 1012 3216
Fragments arranged by size:
3216 1953 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2340 794
Size: 1546 4635
BstBI TT'CG_AA
Cuts at: 3347 4985 3347
Size: 1638 4543
BstEII G'GTnAC_C
Cuts at: 1184 2022 1184
Size: 838 5343
BstXI CCAn_nnnn'nTGG
Cuts at: 2925 2925
Size: 6181
BstYI r'GATC_y
Cuts at: 1972 2618 3511 3878 4263 4280 5732 5818 5830
Size: 646 893 367 385 17 1452 86 12
Cuts at: 5830 6176 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 893 646 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2371 2371
Size: 6181
BtgI C'CryG_G
Cuts at: 513 1065 2004 2056 2918 513
Size: 552 939 52 862 3776
Fragments arranged by size:
3776 939 862 552 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5442
Fragments arranged by size:
5442 683 56
BtsI GCAGTG_nn'
Cuts at: 2416 2715 2890 3697 4491 4511 2416
Size: 299 175 807 794 20 4086
Fragments arranged by size:
4086 807 794 299 175 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2691 2726 529
Size: 35 2127 35 3984
Fragments arranged by size:
3984 2127 35 35
DraI TTT'AAA
Cuts at: 3485 3485
Size: 6181
DraIII CAC_nnn'GTG
Cuts at: 2515 2896 2515
Size: 381 5800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5188 809
Size: 507 3872 1802
Fragments arranged by size:
3872 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2304 3628 4517
Size: 54 1425 50 275 18 474 1324 889
Cuts at: 4517 6146 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1324 889 474 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3628 1537
Size: 275 1816 4090
Fragments arranged by size:
4090 1816 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4111 1353
Size: 2758 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4768 5142 5288 387
Size: 1064 138 3179 374 146 1280
Fragments arranged by size:
3179 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2462 4244 4841 5628 5896 1239
Size: 393 830 1782 597 787 268 1524
Fragments arranged by size:
1782 1524 830 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2177 2177
Size: 6181
EcoO109I rG'GnC_Cy
Cuts at: 2170 2280 2799 3332 2170
Size: 110 519 533 5019
Fragments arranged by size:
5019 533 519 110
EcoRI G'AATT_C
Cuts at: 3490 3868 3490
Size: 378 5803
EcoRV GAT'ATC
Cuts at: 2665 2665
Size: 6181
FspI TGC'GCA
Cuts at: 4687 4687
Size: 6181
HaeII r_GCGC'y
Cuts at: 1678 5328 1678
Size: 3650 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2396 2428 2603 2635 2841
Size: 32 135 32 824 32 175 32 206
Cuts at: 2841 2873 2989 3021 4822 4854 4896 4928 1373
Size: 32 116 32 1801 32 42 32 2626
Fragments arranged by size:
2626 1801 824 206 175 135 116 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2743 3525 3771 669
Size: 988 281 805 782 246 3079
Fragments arranged by size:
3079 988 805 782 281 246
HindIII A'AGCT_T
Cuts at: 748 3405 748
Size: 2657 3524
HpaI GTT'AAC
Cuts at: 3771 3771
Size: 6181
KpnI G_GTAC'C
Cuts at: 2379 3506 2379
Size: 1127 5054
MfeI C'AATT_G
Cuts at: 3780 3780
Size: 6181
MluI A'CGCG_T
Cuts at: 6057 6057
Size: 6181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2211 2370 5295 5479 1305
Size: 906 159 2925 184 2007
Fragments arranged by size:
2925 2007 906 184 159
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4359
Fragments arranged by size:
4359 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2688 3229 4139 4498 4657 518
Size: 648 1522 541 910 359 159 2042
Fragments arranged by size:
2042 1522 910 648 541 359 159
MspA1I CmG'CkG
Cuts at: 1606 2309 2616 3597 4274 5422 5667 1606
Size: 703 307 981 677 1148 245 2120
Fragments arranged by size:
2120 1148 981 703 677 307 245
NaeI GCC'GGC
Cuts at: 2653 3286 2653
Size: 633 5548
NcoI C'CATG_G
Cuts at: 513 1065 2004 2056 2918 513
Size: 552 939 52 862 3776
Fragments arranged by size:
3776 939 862 552 52
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6181
NgoMIV G'CCGG_C
Cuts at: 2651 3284 2651
Size: 633 5548
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6181
NotI GC'GGCC_GC
Cuts at: 3628 3628
Size: 6181
NspI r_CATG'y
Cuts at: 1132 1229 3539 5084 1132
Size: 97 2310 1545 2229
Fragments arranged by size:
2310 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2275 1348
Size: 927 5254
PciI A'CATG_T
Cuts at: 5080 5080
Size: 6181
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2036 2896 1108
Size: 928 860 4393
Fragments arranged by size:
4393 928 860
PfoI T'CCnGG_A
Cuts at: 2222 2222
Size: 6181
PmeI GTTT'AAAC
Cuts at: 3485 3485
Size: 6181
PmlI CAC'GTG
Cuts at: 2842 2842
Size: 6181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4250 4282 5763 5795 4250
Size: 32 1481 32 4636
Fragments arranged by size:
4636 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2170 2280 2170
Size: 110 6071
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6181
PsiI TTA'TAA
Cuts at: 3751 3751
Size: 6181
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2525 2557 2525
Size: 32 6149
PstI C_TGCA'G
Cuts at: 830 2561 3060 3126 3297 3533 830
Size: 1731 499 66 171 236 3478
Fragments arranged by size:
3478 1731 499 236 171 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6181
SalI G'TCGA_C
Cuts at: 1936 3523 1936
Size: 1587 4594
SbfI CC_TGCA'GG
Cuts at: 2561 3297 3533 2561
Size: 736 236 5209
Fragments arranged by size:
5209 736 236
ScaI AGT'ACT
Cuts at: 2135 4429 6141 2135
Size: 2294 1712 2175
Fragments arranged by size:
2294 2175 1712
SfcI C'TryA_G
Cuts at: 826 1045 2557 3056 3122 3293 3529 4664 5345
Size: 219 1512 499 66 171 236 1135 681
Cuts at: 5345 5536 826
Size: 191 1471
Fragments arranged by size:
1512 1471 1135 681 499 236 219 191
171 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6181
SmaI CCC'GGG
Cuts at: 1872 2796 3508 1872
Size: 924 712 4545
Fragments arranged by size:
4545 924 712
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2752 4285 5186 5448 820
Size: 197 926 809 1533 901 262 1553
Fragments arranged by size:
1553 1533 926 901 809 262 197
SnaBI TAC'GTA
Cuts at: 493 3972 493
Size: 3479 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6181
SphI G_CATG'C
Cuts at: 1132 3539 1132
Size: 2407 3774
SspI AAT'ATT
Cuts at: 5 52 4105 5
Size: 47 4053 2081
Fragments arranged by size:
4053 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6181
StyI C'CwwG_G
Cuts at: 513 1065 2004 2056 2596 2918 3204 3620 513
Size: 552 939 52 540 322 286 416 3074
Fragments arranged by size:
3074 939 552 540 416 322 286 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4208 4225 4378 4563 5008 325
Size: 1255 2628 17 153 185 445 1498
Fragments arranged by size:
2628 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2133 2484 4427
Size: 274 80 33 51 1443 156 351 1943
Cuts at: 4427 6139 96
Size: 1712 138
Fragments arranged by size:
1943 1712 1443 351 274 156 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6141 6173 6141
Size: 32 6149
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6181
XbaI T'CTAG_A
Cuts at: 3517 3517
Size: 6181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3323 3323
Size: 6181
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6181
XmaI C'CCGG_G
Cuts at: 1870 2794 3506 1870
Size: 924 712 4545
Fragments arranged by size:
4545 924 712
XmnI GAAnn'nnTTC
Cuts at: 4310 4310
Size: 6181
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3989 276
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoICRI FalI
FseI FspAI KasI NarI NruI NsiI PacI PspOMI
PspXI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI