Restriction map for
pFN21AE4463
(Circular) (Six-base) MAPSORT of: pFN21AE4463.seq Check: 7977 from: 1 to: 6145
pFN21AE4463 6145 bp
With 182 enzymes: SgfI *
March 28, 2012 13:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3955 278
Size: 53 83 186 936 2419 2468
Fragments arranged by size:
2468 2419 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2091 3488 1937
Size: 154 1397 4594
Fragments arranged by size:
4594 1397 154
Acc65I G'GTAC_C
Cuts at: 2112 3466 2112
Size: 1354 4791
AclI AA'CG_TT
Cuts at: 1563 4272 4645 1563
Size: 2709 373 3063
Fragments arranged by size:
3063 2709 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3023 4208 5592 5860 1632
Size: 1391 1185 1384 268 1917
Fragments arranged by size:
1917 1391 1384 1185 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5948
AflIII A'CryG_T
Cuts at: 1203 2157 5044 6021 1203
Size: 954 2887 977 1327
Fragments arranged by size:
2887 1327 977 954
AgeI A'CCGG_T
Cuts at: 4976 4976
Size: 6145
AhdI GACnn_n'nnGTC
Cuts at: 2087 4874 2087
Size: 2787 3358
AleI CACnn'nnGTG
Cuts at: 1166 2462 1166
Size: 1296 4849
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2832 2860 1184
Size: 1648 28 4469
Fragments arranged by size:
4469 1648 28
AlwNI CAG_nnn'CTG
Cuts at: 3888 5460 3888
Size: 1572 4573
ApaLI G'TGCA_C
Cuts at: 4202 5358 4202
Size: 1156 4989
ApoI r'AATT_y
Cuts at: 1493 2018 2926 3454 3680 3832 5941 1493
Size: 525 908 528 226 152 2109 1697
Fragments arranged by size:
2109 1697 908 528 525 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4699 160
Size: 872 3667 1606
Fragments arranged by size:
3667 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
AvaI C'yCGr_G
Cuts at: 1870 1943 2108 2369 3470 1870
Size: 73 165 261 1101 4545
Fragments arranged by size:
4545 1101 261 165 73
BamHI G'GATC_C
Cuts at: 3187 3475 3842 3187
Size: 288 367 5490
Fragments arranged by size:
5490 367 288
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2112 2859 3466 4921 618
Size: 325 217 627 325 747 607 1455 1842
Fragments arranged by size:
1842 1455 747 627 607 325 325 217
BanII G_rGCy'C
Cuts at: 1391 2109 2526 3464 1391
Size: 718 417 938 4072
Fragments arranged by size:
4072 938 718 417
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2802 928
Size: 971 903 4271
Fragments arranged by size:
4271 971 903
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4336 4370 1903
Size: 34 2399 34 3678
Fragments arranged by size:
3678 2399 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4037 5247 4037
Size: 1210 4935
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6145
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2474 4756 136
Size: 107 122 71 631 605 802 2282 1525
Fragments arranged by size:
2282 1525 802 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6140 6140
Size: 6145
BlpI GC'TnA_GC
Cuts at: 3562 3562
Size: 6145
Bme1580I G_kGCm'C
Cuts at: 1790 4206 5362 1790
Size: 2416 1156 2573
Fragments arranged by size:
2573 2416 1156
BmgBI CAC'GTC
Cuts at: 1318 2101 3424 1318
Size: 783 1323 4039
Fragments arranged by size:
4039 1323 783
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3071 3876 4834
Size: 416 53 83 399 24 1654 805 958
Cuts at: 4834 442
Size: 1753
Fragments arranged by size:
1753 1654 958 805 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2366 2436 2702 4805 1239
Size: 1127 70 266 2103 2579
Fragments arranged by size:
2579 2103 1127 266 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4270 5135 5433 4270
Size: 865 298 4982
Fragments arranged by size:
4982 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4808 882
Size: 617 3309 2219
Fragments arranged by size:
3309 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2053 3936 493
Size: 711 849 1883 2702
Fragments arranged by size:
2702 1883 849 711
BsaBI GATnn'nnATC
Cuts at: 6139 6139
Size: 6145
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2083 3952 4334 4969
Size: 53 83 186 936 550 1869 382 635
Cuts at: 4969 275
Size: 1451
Fragments arranged by size:
1869 1451 936 635 550 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4578 4976 5250 5397 5808 4578
Size: 398 274 147 411 4915
Fragments arranged by size:
4915 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2096 2126 1178
Size: 30 165 30 693 30 5197
Fragments arranged by size:
5197 693 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2442 2572 1186
Size: 1256 130 4759
Fragments arranged by size:
4759 1256 130
BseYI C'CCAG_C
Cuts at: 2329 2597 5348 5844 2329
Size: 268 2751 496 2630
Fragments arranged by size:
2751 2630 496 268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2792 3404 1163
Size: 573 1056 612 3904
Fragments arranged by size:
3904 1056 612 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2123 3420 3595 4356 5384 1540
Size: 275 186 122 1297 175 761 1028 2301
Fragments arranged by size:
2301 1297 1028 761 275 186 175 122
BsiHKAI G_wGCw'C
Cuts at: 2526 4206 4291 5362 2526
Size: 1680 85 1071 3309
Fragments arranged by size:
3309 1680 1071 85
BsmI GAATG_Cn'
Cuts at: 2837 3656 3749 2837
Size: 819 93 5233
Fragments arranged by size:
5233 819 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2526 3464 4206 4291 5362 1391
Size: 399 319 417 938 742 85 1071 2174
Fragments arranged by size:
2174 1071 938 742 417 399 319 85
BspEI T'CCGG_A
Cuts at: 5808 5808
Size: 6145
BspHI T'CATG_A
Cuts at: 4034 5764 4034
Size: 1730 4415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2124 3500 844
Size: 1280 1376 3489
Fragments arranged by size:
3489 1376 1280
BsrBI CCG'CTC
Cuts at: 1394 1823 2480 4032 1394
Size: 429 657 1552 3507
Fragments arranged by size:
3507 1552 657 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4640 4814 65
Size: 1183 3392 174 1396
Fragments arranged by size:
3392 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3042 4789 4976 1700
Size: 110 1232 1747 187 2869
Fragments arranged by size:
2869 1747 1232 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6145
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6145
BssSI C'ACGA_G
Cuts at: 4205 5217 4205
Size: 1012 5133
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2834 794
Size: 2040 4105
BstBI TT'CG_AA
Cuts at: 4949 4949
Size: 6145
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6145
BstXI CCAn_nnnn'nTGG
Cuts at: 2065 2464 2065
Size: 399 5746
BstYI r'GATC_y
Cuts at: 1972 3187 3475 3842 4227 4244 5696 5782 5794
Size: 1215 288 367 385 17 1452 86 12
Cuts at: 5794 6140 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1215 385 367 346 288 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2145 3323 2145
Size: 1178 4967
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5593
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5406
Fragments arranged by size:
5406 683 56
BtsI GCAGTG_nn'
Cuts at: 2247 2839 3661 4455 4475 2247
Size: 592 822 794 20 3917
Fragments arranged by size:
3917 822 794 592 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6110
DraI TTT'AAA
Cuts at: 3449 3449
Size: 6145
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3335 5152 809
Size: 507 2019 1817 1802
Fragments arranged by size:
2019 1817 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2120 2265
Size: 54 1425 50 275 18 225 65 145
Cuts at: 2265 2475 3417 3592 4481 6110 8
Size: 210 942 175 889 1629 43
Fragments arranged by size:
1629 1425 942 889 275 225 210 175
145 65 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2120 3417 3592 1537
Size: 275 308 1297 175 4090
Fragments arranged by size:
4090 1297 308 275 175
EarI CTCTTCn'nnn_
Cuts at: 1353 3276 4075 1353
Size: 1923 799 3423
Fragments arranged by size:
3423 1923 799
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4732 5106 5252 387
Size: 1064 138 3143 374 146 1280
Fragments arranged by size:
3143 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2524 2524
Size: 6145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2366 2436 2702 3023 4208 4805 5592
Size: 393 734 70 266 321 1185 597 787
Cuts at: 5592 5860 1239
Size: 268 1524
Fragments arranged by size:
1524 1185 787 734 597 393 321 268
266 70
EcoRI G'AATT_C
Cuts at: 2018 3454 3832 2018
Size: 1436 378 4331
Fragments arranged by size:
4331 1436 378
FspI TGC'GCA
Cuts at: 4651 4651
Size: 6145
HaeII r_GCGC'y
Cuts at: 1678 5292 1678
Size: 3614 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2073 2105 2418 2450 3155
Size: 32 135 32 501 32 313 32 705
Cuts at: 3155 3187 4786 4818 4860 4892 1373
Size: 32 1599 32 42 32 2626
Fragments arranged by size:
2626 1599 705 501 313 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3489 3735 669
Size: 988 281 1551 246 3079
Fragments arranged by size:
3079 1551 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6145
HpaI GTT'AAC
Cuts at: 3735 3735
Size: 6145
KpnI G_GTAC'C
Cuts at: 2116 3470 2116
Size: 1354 4791
MfeI C'AATT_G
Cuts at: 3744 3744
Size: 6145
MluI A'CGCG_T
Cuts at: 6021 6021
Size: 6145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3276 5259 5443 1305
Size: 1971 1983 184 2007
Fragments arranged by size:
2007 1983 1971 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2057 10
Size: 54 1425 343 225 4098
Fragments arranged by size:
4098 1425 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2462 2814 2948 4103 4462 4621 518
Size: 648 1296 352 134 1155 359 159 2042
Fragments arranged by size:
2042 1296 1155 648 359 352 159 134
MspA1I CmG'CkG
Cuts at: 1606 2125 2138 3154 3388 3561 4238 5386 5631
Size: 519 13 1016 234 173 677 1148 245
Cuts at: 5631 1606
Size: 2120
Fragments arranged by size:
2120 1148 1016 677 519 245 234 173
13
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5593
NdeI CA'TA_TG
Cuts at: 387 2801 387
Size: 2414 3731
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6145
NotI GC'GGCC_GC
Cuts at: 2120 3592 2120
Size: 1472 4673
NspI r_CATG'y
Cuts at: 1132 1229 2284 2337 2748 3149 3503 5048 1132
Size: 97 1055 53 411 401 354 1545 2229
Fragments arranged by size:
2229 1545 1055 411 401 354 97 53
PasI CC'CwG_GG
Cuts at: 1348 2731 1348
Size: 1383 4762
PciI A'CATG_T
Cuts at: 5044 5044
Size: 6145
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6145
PmeI GTTT'AAAC
Cuts at: 3449 3449
Size: 6145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4214 4246 5727 5759 4214
Size: 32 1481 32 4600
Fragments arranged by size:
4600 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6145
PsiI TTA'TAA
Cuts at: 3715 3715
Size: 6145
PstI C_TGCA'G
Cuts at: 830 2508 2673 3129 3497 830
Size: 1678 165 456 368 3478
Fragments arranged by size:
3478 1678 456 368 165
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6145
PvuII CAG'CTG
Cuts at: 3154 3154
Size: 6145
SacI G_AGCT'C
Cuts at: 2526 2526
Size: 6145
SalI G'TCGA_C
Cuts at: 1936 3487 1936
Size: 1551 4594
SapI GCTCTTCn'nnn_
Cuts at: 3276 3276
Size: 6145
SbfI CC_TGCA'GG
Cuts at: 2673 3497 2673
Size: 824 5321
ScaI AGT'ACT
Cuts at: 4393 6105 4393
Size: 1712 4433
SfcI C'TryA_G
Cuts at: 826 1045 2504 2669 3125 3493 4628 5309 5500
Size: 219 1459 165 456 368 1135 681 191
Cuts at: 5500 826
Size: 1471
Fragments arranged by size:
1471 1459 1135 681 456 368 219 191
165
SfiI GGCCn_nnn'nGGCC
Cuts at: 2474 2474
Size: 6145
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6145
SmaI CCC'GGG
Cuts at: 1872 2110 3472 1872
Size: 238 1362 4545
Fragments arranged by size:
4545 1362 238
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4249 5150 5412 820
Size: 197 926 2306 901 262 1553
Fragments arranged by size:
2306 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3936 493
Size: 3443 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6145
SphI G_CATG'C
Cuts at: 1132 2337 3503 1132
Size: 1205 1166 3774
Fragments arranged by size:
3774 1205 1166
SspI AAT'ATT
Cuts at: 5 52 4069 5
Size: 47 4017 2081
Fragments arranged by size:
4017 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6145
StyI C'CwwG_G
Cuts at: 513 1065 3584 513
Size: 552 2519 3074
Fragments arranged by size:
3074 2519 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2765 2938 4172 4189 4342 4527 4972
Size: 1255 1185 173 1234 17 153 185 445
Cuts at: 4972 325
Size: 1498
Fragments arranged by size:
1498 1255 1234 1185 445 185 173 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4391 6103 96
Size: 274 80 33 51 1443 2414 1712 138
Fragments arranged by size:
2414 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2270 2302 6105 6137 2270
Size: 32 3803 32 2278
Fragments arranged by size:
3803 2278 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6145
XbaI T'CTAG_A
Cuts at: 3481 3481
Size: 6145
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6145
XmaI C'CCGG_G
Cuts at: 1870 2108 3470 1870
Size: 238 1362 4545
Fragments arranged by size:
4545 1362 238
XmnI GAAnn'nnTTC
Cuts at: 3216 3270 4274 3216
Size: 54 1004 5087
Fragments arranged by size:
5087 1004 54
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3953 276
Size: 53 83 186 936 2419 2468
Fragments arranged by size:
2468 2419 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfoI
SrfI SwaI XcmI