Restriction map for
pFN21AE4462
(Circular) (Six-base) MAPSORT of: pFN21AE4462.seq Check: 915 from: 1 to: 6142
pFN21AE4462 6142 bp
With 182 enzymes: SgfI *
March 28, 2012 14:03 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3952 278
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2658 3198 3485 1937
Size: 721 540 287 4594
Fragments arranged by size:
4594 721 540 287
Acc65I G'GTAC_C
Cuts at: 3463 3463
Size: 6142
AclI AA'CG_TT
Cuts at: 1563 4269 4642 1563
Size: 2706 373 3063
Fragments arranged by size:
3063 2706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2582 2765 3167 4205 5589 5857 1632
Size: 950 183 402 1038 1384 268 1917
Fragments arranged by size:
1917 1384 1038 950 402 268 183
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5945
AflIII A'CryG_T
Cuts at: 1203 3081 5041 6018 1203
Size: 1878 1960 977 1327
Fragments arranged by size:
1960 1878 1327 977
AgeI A'CCGG_T
Cuts at: 4973 4973
Size: 6142
AhdI GACnn_n'nnGTC
Cuts at: 4871 4871
Size: 6142
AleI CACnn'nnGTG
Cuts at: 1166 2888 1166
Size: 1722 4420
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6142
AlwNI CAG_nnn'CTG
Cuts at: 2605 2953 3145 3885 5457 2605
Size: 348 192 740 1572 3290
Fragments arranged by size:
3290 1572 740 348 192
ApaLI G'TGCA_C
Cuts at: 4199 5355 4199
Size: 1156 4986
ApoI r'AATT_y
Cuts at: 1493 3451 3677 3829 5938 1493
Size: 1958 226 152 2109 1697
Fragments arranged by size:
2109 1958 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4696 160
Size: 872 3664 1606
Fragments arranged by size:
3664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
AvaI C'yCGr_G
Cuts at: 1870 1943 2073 2117 2219 3467 1870
Size: 73 130 44 102 1248 4545
Fragments arranged by size:
4545 1248 130 102 73 44
BamHI G'GATC_C
Cuts at: 2215 2581 3472 3839 2215
Size: 366 891 367 4518
Fragments arranged by size:
4518 891 367 366
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2854 3362 3463 4918 618
Size: 325 217 627 1067 508 101 1455 1842
Fragments arranged by size:
1842 1455 1067 627 508 325 217 101
BanII G_rGCy'C
Cuts at: 1391 2283 3461 1391
Size: 892 1178 4072
Fragments arranged by size:
4072 1178 892
BbeI G_GCGC'C
Cuts at: 3366 3366
Size: 6142
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2694 928
Size: 971 795 4376
Fragments arranged by size:
4376 971 795
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4333 4367 1903
Size: 34 2396 34 3678
Fragments arranged by size:
3678 2396 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4034 5244 4034
Size: 1210 4932
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6142
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4753 136
Size: 107 122 71 631 605 3081 1525
Fragments arranged by size:
3081 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6137 6137
Size: 6142
BlpI GC'TnA_GC
Cuts at: 3155 3559 3155
Size: 404 5738
Bme1580I G_kGCm'C
Cuts at: 1790 2859 4203 5359 1790
Size: 1069 1344 1156 2573
Fragments arranged by size:
2573 1344 1156 1069
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6142
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3133 3873 4831
Size: 416 53 83 399 24 1716 740 958
Cuts at: 4831 442
Size: 1753
Fragments arranged by size:
1753 1716 958 740 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2351 2399 2693 2868 4802 1239
Size: 1112 48 294 175 1934 2579
Fragments arranged by size:
2579 1934 1112 294 175 48
Bpu10I CC'TnA_GC
Cuts at: 2962 2962
Size: 6142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2915 4267 5132 5430 2915
Size: 1352 865 298 3627
Fragments arranged by size:
3627 1352 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4805 882
Size: 617 3306 2219
Fragments arranged by size:
3306 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3037 3933 493
Size: 711 1833 896 2702
Fragments arranged by size:
2702 1833 896 711
BsaBI GATnn'nnATC
Cuts at: 6136 6136
Size: 6142
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2069 3363 3949 4331
Size: 53 83 186 936 536 1294 586 382
Cuts at: 4331 4966 275
Size: 635 1451
Fragments arranged by size:
1451 1294 936 635 586 536 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2494 4575 4973 5247 5394 5805 2494
Size: 2081 398 274 147 411 2831
Fragments arranged by size:
2831 2081 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5917
Fragments arranged by size:
5917 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2273 2617 1186
Size: 1087 344 4711
Fragments arranged by size:
4711 1087 344
BseYI C'CCAG_C
Cuts at: 2386 2390 2585 2858 5345 5841 2386
Size: 4 195 273 2487 496 2687
Fragments arranged by size:
2687 2487 496 273 195 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2977 3284 1163
Size: 573 1241 307 4021
Fragments arranged by size:
4021 1241 573 307
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3592 4353 5381 1540
Size: 275 186 1591 761 1028 2301
Fragments arranged by size:
2301 1591 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4203 4288 5359 4203
Size: 85 1071 4986
Fragments arranged by size:
4986 1071 85
BsiWI C'GTAC_G
Cuts at: 3033 3033
Size: 6142
BsmI GAATG_Cn'
Cuts at: 2771 3653 3746 2771
Size: 882 93 5167
Fragments arranged by size:
5167 882 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3122 3122
Size: 6142
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2859 3461 4203 4288 5359 1391
Size: 399 493 576 602 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 602 576 493 399 85
BspEI T'CCGG_A
Cuts at: 2494 5805 2494
Size: 3311 2831
BspHI T'CATG_A
Cuts at: 4031 5761 4031
Size: 1730 4412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3497 844
Size: 2653 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4029 1394
Size: 429 2206 3507
Fragments arranged by size:
3507 2206 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2623 4637 4811 65
Size: 1183 1375 2014 174 1396
Fragments arranged by size:
2014 1396 1375 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3270 3314 3365 4786 4973 1700
Size: 110 1460 44 51 1421 187 2869
Fragments arranged by size:
2869 1460 1421 187 110 51 44
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6142
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6142
BssSI C'ACGA_G
Cuts at: 4202 5214 4202
Size: 1012 5130
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6142
BstBI TT'CG_AA
Cuts at: 4946 4946
Size: 6142
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6142
BstXI CCAn_nnnn'nTGG
Cuts at: 2318 2318
Size: 6142
BstYI r'GATC_y
Cuts at: 1972 2103 2215 2581 3174 3472 3839 4224 4241
Size: 131 112 366 593 298 367 385 17
Cuts at: 4241 5693 5779 5791 6137 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 593 385 367 366 346 298
131 112 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2674 513
Size: 552 1609 3981
Fragments arranged by size:
3981 1609 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3325 487
Size: 683 56 2099 3304
Fragments arranged by size:
3304 2099 683 56
BtsI GCAGTG_nn'
Cuts at: 2716 2951 3658 4452 4472 2716
Size: 235 707 794 20 4386
Fragments arranged by size:
4386 794 707 235 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6107
DraI TTT'AAA
Cuts at: 3446 3446
Size: 6142
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2444 5149 809
Size: 507 1128 2705 1802
Fragments arranged by size:
2705 1802 1128 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 2562 3367
Size: 54 1425 50 275 18 294 438 805
Cuts at: 3367 3589 4478 6107 8
Size: 222 889 1629 43
Fragments arranged by size:
1629 1425 889 805 438 294 275 222
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3589 1537
Size: 275 1777 4090
Fragments arranged by size:
4090 1777 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4072 1353
Size: 2719 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2132 2474 4729 5103 5249 387
Size: 1064 138 543 342 2255 374 146 1280
Fragments arranged by size:
2255 1280 1064 543 374 342 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2351 2399 2582 2693 2765 2868 3167
Size: 393 719 48 183 111 72 103 299
Cuts at: 3167 4205 4802 5589 5857 1239
Size: 1038 597 787 268 1524
Fragments arranged by size:
1524 1038 787 719 597 393 299 268
183 111 103 72 48
EcoO109I rG'GnC_Cy
Cuts at: 2255 2467 3046 2255
Size: 212 579 5351
Fragments arranged by size:
5351 579 212
EcoRI G'AATT_C
Cuts at: 3451 3829 3451
Size: 378 5764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2758 2758
Size: 6142
FspI TGC'GCA
Cuts at: 2978 4648 2978
Size: 1670 4472
FspAI rTGC'GCAy
Cuts at: 2978 2978
Size: 6142
HaeII r_GCGC'y
Cuts at: 1678 2113 3271 3366 5289 1678
Size: 435 1158 95 1923 2531
Fragments arranged by size:
2531 1923 1158 435 95
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2929 2961 4783 4815 4857
Size: 32 135 32 1357 32 1822 32 42
Cuts at: 4857 4889 1373
Size: 32 2626
Fragments arranged by size:
2626 1822 1357 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3486 3732 669
Size: 988 281 1548 246 3079
Fragments arranged by size:
3079 1548 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6142
HpaI GTT'AAC
Cuts at: 3732 3732
Size: 6142
KasI G'GCGC_C
Cuts at: 3362 3362
Size: 6142
KpnI G_GTAC'C
Cuts at: 3467 3467
Size: 6142
MfeI C'AATT_G
Cuts at: 3741 3741
Size: 6142
MluI A'CGCG_T
Cuts at: 6018 6018
Size: 6142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5256 5440 1305
Size: 3951 184 2007
Fragments arranged by size:
3951 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2126 10
Size: 54 1425 343 294 4026
Fragments arranged by size:
4026 1425 343 294 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2888 4100 4459 4618 518
Size: 648 1722 1212 359 159 2042
Fragments arranged by size:
2042 1722 1212 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2029 2390 3008 3067 3300 3558 4235 5383
Size: 423 361 618 59 233 258 677 1148
Cuts at: 5383 5628 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 618 423 361 258 245
233 59
NaeI GCC'GGC
Cuts at: 3272 3316 3367 3272
Size: 44 51 6047
Fragments arranged by size:
6047 51 44
NarI GG'CG_CC
Cuts at: 3363 3363
Size: 6142
NcoI C'CATG_G
Cuts at: 513 1065 2674 513
Size: 552 1609 3981
Fragments arranged by size:
3981 1609 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6142
NgoMIV G'CCGG_C
Cuts at: 3270 3314 3365 3270
Size: 44 51 6047
Fragments arranged by size:
6047 51 44
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6142
NotI GC'GGCC_GC
Cuts at: 3589 3589
Size: 6142
NspI r_CATG'y
Cuts at: 1132 1229 3085 3500 5045 1132
Size: 97 1856 415 1545 2229
Fragments arranged by size:
2229 1856 1545 415 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6142
PciI A'CATG_T
Cuts at: 3081 5041 3081
Size: 1960 4182
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2902 3256 1108
Size: 1794 354 3994
Fragments arranged by size:
3994 1794 354
PfoI T'CCnGG_A
Cuts at: 2895 3057 2895
Size: 162 5980
PmeI GTTT'AAAC
Cuts at: 3446 3446
Size: 6142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 5724 5756 4211
Size: 32 1481 32 4597
Fragments arranged by size:
4597 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2255 2255
Size: 6142
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6142
PsiI TTA'TAA
Cuts at: 3712 3712
Size: 6142
PstI C_TGCA'G
Cuts at: 830 2267 3494 830
Size: 1437 1227 3478
Fragments arranged by size:
3478 1437 1227
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6142
PvuII CAG'CTG
Cuts at: 2390 3008 3067 2390
Size: 618 59 5465
Fragments arranged by size:
5465 618 59
SalI G'TCGA_C
Cuts at: 1936 3484 1936
Size: 1548 4594
SanDI GG'GwC_CC
Cuts at: 2255 2255
Size: 6142
SbfI CC_TGCA'GG
Cuts at: 3494 3494
Size: 6142
ScaI AGT'ACT
Cuts at: 2150 4390 6102 2150
Size: 2240 1712 2190
Fragments arranged by size:
2240 2190 1712
SfcI C'TryA_G
Cuts at: 826 1045 2263 3490 4625 5306 5497 826
Size: 219 1218 1227 1135 681 191 1471
Fragments arranged by size:
1471 1227 1218 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3364 3364
Size: 6142
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6142
SmaI CCC'GGG
Cuts at: 1872 2119 3469 1872
Size: 247 1350 4545
Fragments arranged by size:
4545 1350 247
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2930 4246 5147 5409 820
Size: 197 926 987 1316 901 262 1553
Fragments arranged by size:
1553 1316 987 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3933 493
Size: 3440 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6142
SphI G_CATG'C
Cuts at: 1132 3500 1132
Size: 2368 3774
SspI AAT'ATT
Cuts at: 5 52 4066 5
Size: 47 4014 2081
Fragments arranged by size:
4014 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6142
StyI C'CwwG_G
Cuts at: 513 1065 2674 3581 513
Size: 552 1609 907 3074
Fragments arranged by size:
3074 1609 907 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4169 4186 4339 4524 4969 325
Size: 1255 2589 17 153 185 445 1498
Fragments arranged by size:
2589 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2148 2334 2499
Size: 274 80 33 51 1443 171 186 165
Cuts at: 2499 4388 6100 96
Size: 1889 1712 138
Fragments arranged by size:
1889 1712 1443 274 186 171 165 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6102 6134 6102
Size: 32 6110
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6142
XbaI T'CTAG_A
Cuts at: 3478 3478
Size: 6142
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6142
XmaI C'CCGG_G
Cuts at: 1870 2117 3467 1870
Size: 247 1350 4545
Fragments arranged by size:
4545 1350 247
XmnI GAAnn'nnTTC
Cuts at: 3329 4271 3329
Size: 942 5200
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3950 276
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SapI SexAI SfiI SrfI SwaI
XcmI