Restriction map for
pFN21AE4461
(Circular) (Six-base) MAPSORT of: pFN21AE4461.seq Check: 8640 from: 1 to: 6136
pFN21AE4461 6136 bp
With 182 enzymes: SgfI *
March 28, 2012 13:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3946 278
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3269 3479 1937
Size: 1332 210 4594
Fragments arranged by size:
4594 1332 210
Acc65I G'GTAC_C
Cuts at: 3457 3457
Size: 6136
AclI AA'CG_TT
Cuts at: 1563 4263 4636 1563
Size: 2700 373 3063
Fragments arranged by size:
3063 2700 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2853 3000 3233 4199 5583 5851 1632
Size: 1221 147 233 966 1384 268 1917
Fragments arranged by size:
1917 1384 1221 966 268 233 147
AfeI AGC'GCT
Cuts at: 2672 2672
Size: 6136
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5939
AflIII A'CryG_T
Cuts at: 1203 5035 6012 1203
Size: 3832 977 1327
Fragments arranged by size:
3832 1327 977
AgeI A'CCGG_T
Cuts at: 4967 4967
Size: 6136
AhdI GACnn_n'nnGTC
Cuts at: 4865 4865
Size: 6136
AleI CACnn'nnGTG
Cuts at: 1166 2936 1166
Size: 1770 4366
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2466 1184
Size: 1282 4854
AlwNI CAG_nnn'CTG
Cuts at: 2502 2550 2942 3133 3166 3879 5451 2502
Size: 48 392 191 33 713 1572 3187
Fragments arranged by size:
3187 1572 713 392 191 48 33
ApaI G_GGCC'C
Cuts at: 2077 2212 3184 2077
Size: 135 972 5029
Fragments arranged by size:
5029 972 135
ApaLI G'TGCA_C
Cuts at: 2441 4193 5349 2441
Size: 1752 1156 3228
Fragments arranged by size:
3228 1752 1156
ApoI r'AATT_y
Cuts at: 1493 3445 3671 3823 5932 1493
Size: 1952 226 152 2109 1697
Fragments arranged by size:
2109 1952 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4690 160
Size: 872 3658 1606
Fragments arranged by size:
3658 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
AvaI C'yCGr_G
Cuts at: 1870 1943 2178 2415 3461 1870
Size: 73 235 237 1046 4545
Fragments arranged by size:
4545 1046 237 235 73
AvrII C'CTAG_G
Cuts at: 2203 2203
Size: 6136
BamHI G'GATC_C
Cuts at: 3466 3833 3466
Size: 367 5769
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2312 3007 3393 3457 4912
Size: 325 217 627 525 695 386 64 1455
Cuts at: 4912 618
Size: 1842
Fragments arranged by size:
1842 1455 695 627 525 386 325 217
64
BanII G_rGCy'C
Cuts at: 1391 2077 2203 2212 2538 2784 2977 3083 3184
Size: 686 126 9 326 246 193 106 101
Cuts at: 3184 3455 1391
Size: 271 4072
Fragments arranged by size:
4072 686 326 271 246 193 126 106
101 9
BbeI G_GCGC'C
Cuts at: 3397 3397
Size: 6136
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5165
BbvCI CC'TCA_GC
Cuts at: 2396 2895 2396
Size: 499 5637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2567 2601 4327 4361 1903
Size: 34 630 34 1726 34 3678
Fragments arranged by size:
3678 1726 630 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4028 5238 4028
Size: 1210 4926
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6136
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2207 2909 3087
Size: 107 122 71 631 605 535 702 178
Cuts at: 3087 4747 136
Size: 1660 1525
Fragments arranged by size:
1660 1525 702 631 605 535 178 122
107 71
BglII A'GATC_T
Cuts at: 6131 6131
Size: 6136
BlpI GC'TnA_GC
Cuts at: 2537 2574 2667 2783 3553 2537
Size: 37 93 116 770 5120
Fragments arranged by size:
5120 770 116 93 37
Bme1580I G_kGCm'C
Cuts at: 1790 2077 2212 2317 2445 2920 3184 3422 4197
Size: 287 135 105 128 475 264 238 775
Cuts at: 4197 5353 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 775 475 287 264 238 135
128 105
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6136
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2553 2609 3259
Size: 416 53 83 399 24 1136 56 650
Cuts at: 3259 3867 4825 442
Size: 608 958 1753
Fragments arranged by size:
1753 1136 958 650 608 416 399 83
56 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2695 2774 2855 3155 3366 4796 1239
Size: 1456 79 81 300 211 1430 2579
Fragments arranged by size:
2579 1456 1430 300 211 81 79
Bpu10I CC'TnA_GC
Cuts at: 2066 2396 2895 3066 2066
Size: 330 499 171 5136
Fragments arranged by size:
5136 499 330 171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4261 5126 5424 4261
Size: 865 298 4973
Fragments arranged by size:
4973 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2265 4799 882
Size: 617 766 2534 2219
Fragments arranged by size:
2534 2219 766 617
BsaAI yAC'GTr
Cuts at: 493 1204 2305 3927 493
Size: 711 1101 1622 2702
Fragments arranged by size:
2702 1622 1101 711
BsaBI GATnn'nnATC
Cuts at: 6130 6130
Size: 6136
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2480 3394 3943 4325
Size: 53 83 186 936 947 914 549 382
Cuts at: 4325 4960 275
Size: 635 1451
Fragments arranged by size:
1451 947 936 914 635 549 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4569 4967 5241 5388 5799 4569
Size: 398 274 147 411 4906
Fragments arranged by size:
4906 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3354 3384 1178
Size: 30 165 30 1951 30 3930
Fragments arranged by size:
3930 1951 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6136
BseYI C'CCAG_C
Cuts at: 2026 2278 2390 2454 2611 3109 5339 5835 2026
Size: 252 112 64 157 498 2230 496 2327
Fragments arranged by size:
2327 2230 498 496 252 157 112 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5563
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3586 4347 5375 1540
Size: 275 186 1585 761 1028 2301
Fragments arranged by size:
2301 1585 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2445 2538 2877 4197 4282 5353 2445
Size: 93 339 1320 85 1071 3228
Fragments arranged by size:
3228 1320 1071 339 93 85
BsmI GAATG_Cn'
Cuts at: 3258 3647 3740 3258
Size: 389 93 5654
Fragments arranged by size:
5654 389 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2487 2487
Size: 6136
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2203 2212 2317 2445 2538 2784
Size: 399 287 126 9 105 128 93 246
Cuts at: 2784 2877 2920 2977 3083 3184 3422 3455 4197
Size: 93 43 57 106 101 238 33 742
Cuts at: 4197 4282 5353 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 287 246 238 128
126 106 105 101 93 93 85 57
43 33 9
BspEI T'CCGG_A
Cuts at: 5799 5799
Size: 6136
BspHI T'CATG_A
Cuts at: 4025 5755 4025
Size: 1730 4406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3491 844
Size: 2647 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4023 1394
Size: 429 2200 3507
Fragments arranged by size:
3507 2200 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4631 4805 65
Size: 1183 3383 174 1396
Fragments arranged by size:
3383 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2770 4780 4967 1700
Size: 110 960 2010 187 2869
Fragments arranged by size:
2869 2010 960 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6136
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6136
BssSI C'ACGA_G
Cuts at: 2913 4196 5208 2913
Size: 1283 1012 3841
Fragments arranged by size:
3841 1283 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6136
BstBI TT'CG_AA
Cuts at: 4940 4940
Size: 6136
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6136
BstXI CCAn_nnnn'nTGG
Cuts at: 2599 2996 2599
Size: 397 5739
BstYI r'GATC_y
Cuts at: 1972 3022 3466 3833 4218 4235 5687 5773 5785
Size: 1050 444 367 385 17 1452 86 12
Cuts at: 5785 6131 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1050 444 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3270 3270
Size: 6136
Bsu36I CC'TnA_GG
Cuts at: 2088 2339 2472 2715 2088
Size: 251 133 243 5509
Fragments arranged by size:
5509 251 243 133
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2490 2506 513
Size: 552 939 12 474 16 4143
Fragments arranged by size:
4143 939 552 474 16 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5397
Fragments arranged by size:
5397 683 56
BtsI GCAGTG_nn'
Cuts at: 2116 2931 2943 3229 3652 4446 4466 2116
Size: 815 12 286 423 794 20 3786
Fragments arranged by size:
3786 815 794 423 286 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6101
DraI TTT'AAA
Cuts at: 3440 3440
Size: 6136
DraIII CAC_nnn'GTG
Cuts at: 2693 2693
Size: 6136
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5143 809
Size: 507 3827 1802
Fragments arranged by size:
3827 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 3583 4472
Size: 54 1425 50 275 18 219 1534 889
Cuts at: 4472 6101 8
Size: 1629 43
Fragments arranged by size:
1629 1534 1425 889 275 219 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3583 1537
Size: 275 1771 4090
Fragments arranged by size:
4090 1771 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2570 4066 1353
Size: 1217 1496 3423
Fragments arranged by size:
3423 1496 1217
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2046 2342 2638 4723 5097 5243
Size: 1064 138 457 296 296 2085 374 146
Cuts at: 5243 387
Size: 1280
Fragments arranged by size:
2085 1280 1064 457 374 296 296 146
138
EcoICRI GAG'CTC
Cuts at: 2536 2536
Size: 6136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2695 2774 2853 2855 3000 3155 3233
Size: 393 1063 79 79 2 145 155 78
Cuts at: 3233 3366 4199 4796 5583 5851 1239
Size: 133 833 597 787 268 1524
Fragments arranged by size:
1524 1063 833 787 597 393 268 155
145 133 79 79 78 2
EcoO109I rG'GnC_Cy
Cuts at: 2074 2147 2208 2547 2719 2074
Size: 73 61 339 172 5491
Fragments arranged by size:
5491 339 172 73 61
EcoRI G'AATT_C
Cuts at: 3445 3823 3445
Size: 378 5758
FspI TGC'GCA
Cuts at: 4642 4642
Size: 6136
HaeII r_GCGC'y
Cuts at: 1678 2461 2674 2806 3397 5283 1678
Size: 783 213 132 591 1886 2531
Fragments arranged by size:
2531 1886 783 591 213 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2465 2497 2698 2730 4777
Size: 32 135 32 893 32 201 32 2047
Cuts at: 4777 4809 4851 4883 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2047 893 201 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3480 3726 669
Size: 988 281 1542 246 3079
Fragments arranged by size:
3079 1542 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6136
HpaI GTT'AAC
Cuts at: 3726 3726
Size: 6136
KasI G'GCGC_C
Cuts at: 3393 3393
Size: 6136
KpnI G_GTAC'C
Cuts at: 3461 3461
Size: 6136
MfeI C'AATT_G
Cuts at: 3735 3735
Size: 6136
MluI A'CGCG_T
Cuts at: 6012 6012
Size: 6136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5250 5434 1305
Size: 3945 184 2007
Fragments arranged by size:
3945 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4314
Fragments arranged by size:
4314 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2936 4094 4453 4612 518
Size: 648 1770 1158 359 159 2042
Fragments arranged by size:
2042 1770 1158 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2043 2195 2282 3178 3194 3552 4229 5377
Size: 437 152 87 896 16 358 677 1148
Cuts at: 5377 5622 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 896 677 437 358 245 152
87 16
NarI GG'CG_CC
Cuts at: 3394 3394
Size: 6136
NcoI C'CATG_G
Cuts at: 513 1065 2004 2490 513
Size: 552 939 486 4159
Fragments arranged by size:
4159 939 552 486
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6136
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6136
NotI GC'GGCC_GC
Cuts at: 3583 3583
Size: 6136
NspI r_CATG'y
Cuts at: 1132 1229 2324 3494 5039 1132
Size: 97 1095 1170 1545 2229
Fragments arranged by size:
2229 1545 1170 1095 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6136
PciI A'CATG_T
Cuts at: 5035 5035
Size: 6136
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2662 3235 1108
Size: 1554 573 4009
Fragments arranged by size:
4009 1554 573
PfoI T'CCnGG_A
Cuts at: 3025 3025
Size: 6136
PmeI GTTT'AAAC
Cuts at: 3440 3440
Size: 6136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4205 4237 5718 5750 4205
Size: 32 1481 32 4591
Fragments arranged by size:
4591 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2547 2547
Size: 6136
PshAI GACnn'nnGTC
Cuts at: 1613 2018 2829 1613
Size: 405 811 4920
Fragments arranged by size:
4920 811 405
PsiI TTA'TAA
Cuts at: 3706 3706
Size: 6136
PspOMI G'GGCC_C
Cuts at: 2073 2208 3180 2073
Size: 135 972 5029
Fragments arranged by size:
5029 972 135
PstI C_TGCA'G
Cuts at: 830 2938 2964 3488 830
Size: 2108 26 524 3478
Fragments arranged by size:
3478 2108 524 26
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6136
PvuII CAG'CTG
Cuts at: 2282 3194 2282
Size: 912 5224
RsrII CG'GwC_CG
Cuts at: 2013 2013
Size: 6136
SacI G_AGCT'C
Cuts at: 2538 2538
Size: 6136
SalI G'TCGA_C
Cuts at: 1936 3478 1936
Size: 1542 4594
SbfI CC_TGCA'GG
Cuts at: 3488 3488
Size: 6136
ScaI AGT'ACT
Cuts at: 4384 6096 4384
Size: 1712 4424
SfcI C'TryA_G
Cuts at: 826 1045 2934 2943 2960 3484 4619 5300 5491
Size: 219 1889 9 17 524 1135 681 191
Cuts at: 5491 826
Size: 1471
Fragments arranged by size:
1889 1471 1135 681 524 219 191 17
9
SfoI GGC'GCC
Cuts at: 3395 3395
Size: 6136
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6136
SmaI CCC'GGG
Cuts at: 1872 2417 3463 1872
Size: 545 1046 4545
Fragments arranged by size:
4545 1046 545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4240 5141 5403 820
Size: 197 926 2297 901 262 1553
Fragments arranged by size:
2297 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3927 493
Size: 3434 2702
SpeI A'CTAG_T
Cuts at: 152 2738 152
Size: 2586 3550
SphI G_CATG'C
Cuts at: 1132 2324 3494 1132
Size: 1192 1170 3774
Fragments arranged by size:
3774 1192 1170
SspI AAT'ATT
Cuts at: 5 52 4060 5
Size: 47 4008 2081
Fragments arranged by size:
4008 2081 47
StuI AGG'CCT
Cuts at: 1517 2660 1517
Size: 1143 4993
StyI C'CwwG_G
Cuts at: 513 1065 2004 2203 2464 2490 2661 2968 3575
Size: 552 939 199 261 26 171 307 607
Cuts at: 3575 513
Size: 3074
Fragments arranged by size:
3074 939 607 552 307 261 199 171
26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2292 4163 4180 4333 4518 4963 325
Size: 1255 712 1871 17 153 185 445 1498
Fragments arranged by size:
1871 1498 1255 712 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4382 6094 96
Size: 274 80 33 51 1443 2405 1712 138
Fragments arranged by size:
2405 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2460 2492 2991 3023 6096 6128 2460
Size: 32 499 32 3073 32 2468
Fragments arranged by size:
3073 2468 499 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6136
XbaI T'CTAG_A
Cuts at: 2701 3472 2701
Size: 771 5365
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2462 2774 2462
Size: 312 5824
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6136
XmaI C'CCGG_G
Cuts at: 1870 2415 3461 1870
Size: 545 1046 4545
Fragments arranged by size:
4545 1046 545
XmnI GAAnn'nnTTC
Cuts at: 2294 4265 2294
Size: 1971 4165
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3944 276
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BfrBI BplI BsiWI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI SacII SanDI SapI SexAI
SfiI SrfI SwaI