Restriction map for
pFN21AE4459
(Circular) (Six-base) MAPSORT of: pFN21AE4459.seq Check: 67 from: 1 to: 6133
pFN21AE4459 6133 bp
With 182 enzymes: SgfI *
March 28, 2012 14:03 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3943 278
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3476 1937
Size: 1539 4594
Acc65I G'GTAC_C
Cuts at: 3454 3454
Size: 6133
AclI AA'CG_TT
Cuts at: 1563 4260 4633 1563
Size: 2697 373 3063
Fragments arranged by size:
3063 2697 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3075 3086 4196 5580 5848 1632
Size: 1443 11 1110 1384 268 1917
Fragments arranged by size:
1917 1443 1384 1110 268 11
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5936
AflIII A'CryG_T
Cuts at: 1203 5032 6009 1203
Size: 3829 977 1327
Fragments arranged by size:
3829 1327 977
AgeI A'CCGG_T
Cuts at: 4964 4964
Size: 6133
AhdI GACnn_n'nnGTC
Cuts at: 4862 4862
Size: 6133
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2045 1184
Size: 861 5272
AlwNI CAG_nnn'CTG
Cuts at: 3009 3876 5448 3009
Size: 867 1572 3694
Fragments arranged by size:
3694 1572 867
ApaLI G'TGCA_C
Cuts at: 2212 4190 5346 2212
Size: 1978 1156 2999
Fragments arranged by size:
2999 1978 1156
ApoI r'AATT_y
Cuts at: 1493 2321 3427 3442 3668 3820 5929 1493
Size: 828 1106 15 226 152 2109 1697
Fragments arranged by size:
2109 1697 1106 828 226 152 15
AseI AT'TA_AT
Cuts at: 160 1032 4687 160
Size: 872 3655 1606
Fragments arranged by size:
3655 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
AvaI C'yCGr_G
Cuts at: 1870 1943 2093 2174 2850 3458 1870
Size: 73 150 81 676 608 4545
Fragments arranged by size:
4545 676 608 150 81 73
BamHI G'GATC_C
Cuts at: 3463 3830 3463
Size: 367 5766
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 2098 2121 3454 4909
Size: 325 217 627 288 23 23 1333 1455
Cuts at: 4909 618
Size: 1842
Fragments arranged by size:
1842 1455 1333 627 325 288 217 23
23
BanII G_rGCy'C
Cuts at: 1391 2094 2268 3452 1391
Size: 703 174 1184 4072
Fragments arranged by size:
4072 1184 703 174
BbeI G_GCGC'C
Cuts at: 2079 2102 2125 2079
Size: 23 23 6087
Fragments arranged by size:
6087 23 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3074 928
Size: 971 1175 3987
Fragments arranged by size:
3987 1175 971
BbvCI CC'TCA_GC
Cuts at: 3274 3274
Size: 6133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2137 2171 4324 4358 1903
Size: 34 200 34 2153 34 3678
Fragments arranged by size:
3678 2153 200 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4025 5235 4025
Size: 1210 4923
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6133
BfrBI ATG'CAT
Cuts at: 2344 2344
Size: 6133
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4744 136
Size: 107 122 71 631 605 3072 1525
Fragments arranged by size:
3072 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2560 6128 2560
Size: 3568 2565
BlpI GC'TnA_GC
Cuts at: 3032 3550 3032
Size: 518 5615
Bme1580I G_kGCm'C
Cuts at: 1790 2050 2216 4194 5350 1790
Size: 260 166 1978 1156 2573
Fragments arranged by size:
2573 1978 1156 260 166
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6133
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2383 3085 3864
Size: 416 53 83 399 24 966 702 779
Cuts at: 3864 4822 442
Size: 958 1753
Fragments arranged by size:
1753 966 958 779 702 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6133
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2390 2390
Size: 6133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2994 3256 3264 3371 4793 1239
Size: 1755 262 8 107 1422 2579
Fragments arranged by size:
2579 1755 1422 262 107 8
Bpu10I CC'TnA_GC
Cuts at: 2999 3274 2999
Size: 275 5858
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2504 3131 4258 5123 5421 2504
Size: 627 1127 865 298 3216
Fragments arranged by size:
3216 1127 865 627 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2681 2748 3141 4796 882
Size: 617 1182 67 393 1655 2219
Fragments arranged by size:
2219 1655 1182 617 393 67
BsaAI yAC'GTr
Cuts at: 493 1204 2602 3924 493
Size: 711 1398 1322 2702
Fragments arranged by size:
2702 1398 1322 711
BsaBI GATnn'nnATC
Cuts at: 6127 6127
Size: 6133
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 2099 2122 2298
Size: 53 83 186 936 543 23 23 176
Cuts at: 2298 2829 3940 4322 4957 275
Size: 531 1111 382 635 1451
Fragments arranged by size:
1451 1111 936 635 543 531 382 186
176 83 53 23 23
BsaWI w'CCGG_w
Cuts at: 2568 4566 4964 5238 5385 5796 2568
Size: 1998 398 274 147 411 2905
Fragments arranged by size:
2905 1998 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2135 2165 2745 2775 2782
Size: 30 165 30 732 30 580 30 7
Cuts at: 2782 2801 2812 2831 3077 3107 1178
Size: 19 11 19 246 30 4204
Fragments arranged by size:
4204 732 580 246 165 30 30 30
30 30 19 19 11 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2766 2806 3141 3392 1186
Size: 1580 40 335 251 3927
Fragments arranged by size:
3927 1580 335 251 40
BseYI C'CCAG_C
Cuts at: 3332 5336 5832 3332
Size: 2004 496 3633
Fragments arranged by size:
3633 2004 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5560
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3583 4344 5372 1540
Size: 275 186 1582 761 1028 2301
Fragments arranged by size:
2301 1582 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2216 2268 2803 4194 4279 5350 2216
Size: 52 535 1391 85 1071 2999
Fragments arranged by size:
2999 1391 1071 535 85 52
BsmI GAATG_Cn'
Cuts at: 2454 3178 3644 3737 2454
Size: 724 466 93 4850
Fragments arranged by size:
4850 724 466 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2508 2777 2508
Size: 269 5864
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2094 2216 2268 2803 3452 4194
Size: 399 260 44 122 52 535 649 742
Cuts at: 4194 4279 5350 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 649 535 399 260 122
85 52 44
BspEI T'CCGG_A
Cuts at: 5796 5796
Size: 6133
BspHI T'CATG_A
Cuts at: 2803 4022 5752 2803
Size: 1219 1730 3184
Fragments arranged by size:
3184 1730 1219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3488 844
Size: 2644 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4020 1394
Size: 429 2197 3507
Fragments arranged by size:
3507 2197 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2551 4628 4802 65
Size: 1183 1303 2077 174 1396
Fragments arranged by size:
2077 1396 1303 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2124 4777 4964 1700
Size: 110 314 2653 187 2869
Fragments arranged by size:
2869 2653 314 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6133
BssHII G'CGCG_C
Cuts at: 1925 2025 1925
Size: 100 6033
BssSI C'ACGA_G
Cuts at: 2192 4193 5205 2192
Size: 2001 1012 3120
Fragments arranged by size:
3120 2001 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2054 794
Size: 1260 4873
BstBI TT'CG_AA
Cuts at: 4937 4937
Size: 6133
BstEII G'GTnAC_C
Cuts at: 1184 3158 1184
Size: 1974 4159
BstXI CCAn_nnnn'nTGG
Cuts at: 2309 3187 2309
Size: 878 5255
BstYI r'GATC_y
Cuts at: 1972 2560 3463 3830 4215 4232 5684 5770 5782
Size: 588 903 367 385 17 1452 86 12
Cuts at: 5782 6128 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 903 588 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2103 2746 3024 3186 513
Size: 552 939 99 643 278 162 3460
Fragments arranged by size:
3460 939 643 552 278 162 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5394
Fragments arranged by size:
5394 683 56
BtsI GCAGTG_nn'
Cuts at: 2216 3649 4443 4463 2216
Size: 1433 794 20 3886
Fragments arranged by size:
3886 1433 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3288 3323 529
Size: 35 2724 35 3339
Fragments arranged by size:
3339 2724 35 35
DraI TTT'AAA
Cuts at: 2326 3426 3437 2326
Size: 1100 11 5022
Fragments arranged by size:
5022 1100 11
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5140 809
Size: 507 3824 1802
Fragments arranged by size:
3824 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2763 2874
Size: 54 1425 50 275 18 177 756 111
Cuts at: 2874 2894 3580 4469 6098 8
Size: 20 686 889 1629 43
Fragments arranged by size:
1629 1425 889 756 686 275 177 111
54 50 43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 3580 1537
Size: 275 1768 4090
Fragments arranged by size:
4090 1768 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2866 3051 3369 4063 1353
Size: 1513 185 318 694 3423
Fragments arranged by size:
3423 1513 694 318 185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4720 5094 5240 387
Size: 1064 138 3131 374 146 1280
Fragments arranged by size:
3131 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2266 2266
Size: 6133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2994 3075 3086 3256 3264 3371 4196
Size: 393 1362 81 11 170 8 107 825
Cuts at: 4196 4793 5580 5848 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1362 825 787 597 393 268 170
107 81 11 8
EcoRI G'AATT_C
Cuts at: 3442 3820 3442
Size: 378 5755
FspI TGC'GCA
Cuts at: 4639 4639
Size: 6133
HaeII r_GCGC'y
Cuts at: 1678 2079 2102 2125 2192 5280 1678
Size: 401 23 23 67 3088 2531
Fragments arranged by size:
3088 2531 401 67 23 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 3197 3229 4774
Size: 32 135 32 563 32 1030 32 1545
Cuts at: 4774 4806 4848 4880 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1545 1030 563 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2434 3477 3723 669
Size: 988 281 496 1043 246 3079
Fragments arranged by size:
3079 1043 988 496 281 246
HindIII A'AGCT_T
Cuts at: 748 2675 2842 748
Size: 1927 167 4039
Fragments arranged by size:
4039 1927 167
HpaI GTT'AAC
Cuts at: 3723 3723
Size: 6133
KasI G'GCGC_C
Cuts at: 2075 2098 2121 2075
Size: 23 23 6087
Fragments arranged by size:
6087 23 23
KpnI G_GTAC'C
Cuts at: 3458 3458
Size: 6133
MfeI C'AATT_G
Cuts at: 2427 3165 3732 2427
Size: 738 567 4828
Fragments arranged by size:
4828 738 567
MluI A'CGCG_T
Cuts at: 6009 6009
Size: 6133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2310 2341 5247 5431 1305
Size: 1005 31 2906 184 2007
Fragments arranged by size:
2906 2007 1005 184 31
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2765 10
Size: 54 1425 343 933 3378
Fragments arranged by size:
3378 1425 933 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2349 3185 4091 4450 4609 518
Size: 648 1183 836 906 359 159 2042
Fragments arranged by size:
2042 1183 906 836 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2105 3549 4226 5374 5619 1606
Size: 499 1444 677 1148 245 2120
Fragments arranged by size:
2120 1444 1148 677 499 245
NaeI GCC'GGC
Cuts at: 2126 2126
Size: 6133
NarI GG'CG_CC
Cuts at: 2076 2099 2122 2076
Size: 23 23 6087
Fragments arranged by size:
6087 23 23
NcoI C'CATG_G
Cuts at: 513 1065 2004 2746 513
Size: 552 939 742 3900
Fragments arranged by size:
3900 939 742 552
NdeI CA'TA_TG
Cuts at: 387 2776 387
Size: 2389 3744
NgoMIV G'CCGG_C
Cuts at: 2124 2124
Size: 6133
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6133
NotI GC'GGCC_GC
Cuts at: 3580 3580
Size: 6133
NsiI A_TGCA'T
Cuts at: 2346 2346
Size: 6133
NspI r_CATG'y
Cuts at: 1132 1229 3491 5036 1132
Size: 97 2262 1545 2229
Fragments arranged by size:
2262 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2551 1348
Size: 1203 4930
PciI A'CATG_T
Cuts at: 5032 5032
Size: 6133
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2845 1108
Size: 1737 4396
PmeI GTTT'AAAC
Cuts at: 3437 3437
Size: 6133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4202 4234 5715 5747 4202
Size: 32 1481 32 4588
Fragments arranged by size:
4588 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2429 1613
Size: 816 5317
PsiI TTA'TAA
Cuts at: 3703 3703
Size: 6133
PstI C_TGCA'G
Cuts at: 830 2136 2211 3485 830
Size: 1306 75 1274 3478
Fragments arranged by size:
3478 1306 1274 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6133
SacI G_AGCT'C
Cuts at: 2268 2268
Size: 6133
SacII CC_GC'GG
Cuts at: 2106 2106
Size: 6133
SalI G'TCGA_C
Cuts at: 1936 3475 1936
Size: 1539 4594
SbfI CC_TGCA'GG
Cuts at: 3485 3485
Size: 6133
ScaI AGT'ACT
Cuts at: 4381 6093 4381
Size: 1712 4421
SfcI C'TryA_G
Cuts at: 826 1045 2132 2207 3481 4616 5297 5488 826
Size: 219 1087 75 1274 1135 681 191 1471
Fragments arranged by size:
1471 1274 1135 1087 681 219 191 75
SfoI GGC'GCC
Cuts at: 2077 2100 2123 2077
Size: 23 23 6087
Fragments arranged by size:
6087 23 23
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
SgrAI Cr'CCGG_yG
Cuts at: 1700 2124 1700
Size: 424 5709
SmaI CCC'GGG
Cuts at: 1872 2095 2852 3460 1872
Size: 223 757 608 4545
Fragments arranged by size:
4545 757 608 223
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2519 3110 4237 5138 5400 820
Size: 197 926 576 591 1127 901 262 1553
Fragments arranged by size:
1553 1127 926 901 591 576 262 197
SnaBI TAC'GTA
Cuts at: 493 3924 493
Size: 3431 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6133
SphI G_CATG'C
Cuts at: 1132 3491 1132
Size: 2359 3774
SrfI GCCC'GGGC
Cuts at: 2852 2852
Size: 6133
SspI AAT'ATT
Cuts at: 5 52 4057 5
Size: 47 4005 2081
Fragments arranged by size:
4005 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6133
StyI C'CwwG_G
Cuts at: 513 1065 2004 2691 2746 3572 513
Size: 552 939 687 55 826 3074
Fragments arranged by size:
3074 939 826 687 552 55
SwaI ATTT'AAAT
Cuts at: 2326 2326
Size: 6133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4160 4177 4330 4515 4960 325
Size: 1255 2580 17 153 185 445 1498
Fragments arranged by size:
2580 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4379 6091 96
Size: 274 80 33 51 1443 2402 1712 138
Fragments arranged by size:
2402 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2745 2777 2782 2814 6093 6125 2745
Size: 32 5 32 3279 32 2753
Fragments arranged by size:
3279 2753 32 32 32 5
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6133
XbaI T'CTAG_A
Cuts at: 3469 3469
Size: 6133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3184 3184
Size: 6133
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6133
XmaI C'CCGG_G
Cuts at: 1870 2093 2850 3458 1870
Size: 223 757 608 4545
Fragments arranged by size:
4545 757 608 223
XmnI GAAnn'nnTTC
Cuts at: 4262 4262
Size: 6133
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3941 276
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SacI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BsiWI
BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI
FspAI NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SanDI SapI SexAI SfiI