Restriction map for
pFN21AE4455
(Circular) (Six-base) MAPSORT of: pFN21AE4455.seq Check: 5157 from: 1 to: 6130
pFN21AE4455 6130 bp
With 182 enzymes: SgfI *
March 28, 2012 13:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2478 3291 2478
Size: 813 5317
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3940 278
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2166 3473 1937
Size: 229 1307 4594
Fragments arranged by size:
4594 1307 229
Acc65I G'GTAC_C
Cuts at: 2874 2916 3451 2874
Size: 42 535 5553
Fragments arranged by size:
5553 535 42
AclI AA'CG_TT
Cuts at: 1563 4257 4630 1563
Size: 2694 373 3063
Fragments arranged by size:
3063 2694 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2900 4193 5577 5845 1632
Size: 1268 1293 1384 268 1917
Fragments arranged by size:
1917 1384 1293 1268 268
AfeI AGC'GCT
Cuts at: 3386 3386
Size: 6130
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5933
AflIII A'CryG_T
Cuts at: 1203 2337 5029 6006 1203
Size: 1134 2692 977 1327
Fragments arranged by size:
2692 1327 1134 977
AgeI A'CCGG_T
Cuts at: 4961 4961
Size: 6130
AhdI GACnn_n'nnGTC
Cuts at: 4859 4859
Size: 6130
AleI CACnn'nnGTG
Cuts at: 1166 2545 1166
Size: 1379 4751
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6130
AlwNI CAG_nnn'CTG
Cuts at: 2628 2971 3873 5445 2628
Size: 343 902 1572 3313
Fragments arranged by size:
3313 1572 902 343
ApaLI G'TGCA_C
Cuts at: 4187 5343 4187
Size: 1156 4974
ApoI r'AATT_y
Cuts at: 1493 2633 3439 3665 3817 5926 1493
Size: 1140 806 226 152 2109 1697
Fragments arranged by size:
2109 1697 1140 806 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4684 160
Size: 872 3652 1606
Fragments arranged by size:
3652 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 2732 3455 1870
Size: 73 789 723 4545
Fragments arranged by size:
4545 789 723 73
BamHI G'GATC_C
Cuts at: 2535 3460 3827 2535
Size: 925 367 4838
Fragments arranged by size:
4838 925 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2727 2874 2916 3171 3451
Size: 325 217 627 940 147 42 255 280
Cuts at: 3451 4906 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 940 627 325 280 255 217
147 42
BanII G_rGCy'C
Cuts at: 1391 2790 3449 1391
Size: 1399 659 4072
Fragments arranged by size:
4072 1399 659
BbeI G_GCGC'C
Cuts at: 2731 2731
Size: 6130
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2888 928
Size: 971 989 4170
Fragments arranged by size:
4170 989 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4321 4355 1903
Size: 34 2384 34 3678
Fragments arranged by size:
3678 2384 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4022 5232 4022
Size: 1210 4920
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3042 4741 136
Size: 107 122 71 631 605 1370 1699 1525
Fragments arranged by size:
1699 1525 1370 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6125 6125
Size: 6130
BlpI GC'TnA_GC
Cuts at: 3547 3547
Size: 6130
Bme1580I G_kGCm'C
Cuts at: 1790 4191 5347 1790
Size: 2401 1156 2573
Fragments arranged by size:
2573 2401 1156
BmgBI CAC'GTC
Cuts at: 1318 2340 1318
Size: 1022 5108
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3388 3861 4819
Size: 416 53 83 399 24 1971 473 958
Cuts at: 4819 442
Size: 1753
Fragments arranged by size:
1971 1753 958 473 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4790 1239
Size: 3551 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2064 4255 5120 5418 2064
Size: 2191 865 298 2776
Fragments arranged by size:
2776 2191 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4793 882
Size: 617 3294 2219
Fragments arranged by size:
3294 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2380 2845 3921 493
Size: 711 1176 465 1076 2702
Fragments arranged by size:
2702 1176 1076 711 465
BsaBI GATnn'nnATC
Cuts at: 2669 6124 2669
Size: 3455 2675
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 2728 3021 3937
Size: 53 83 186 936 522 673 293 916
Cuts at: 3937 4319 4954 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 916 673 635 522 382 293
186 83 53
BsaWI w'CCGG_w
Cuts at: 4563 4961 5235 5382 5793 4563
Size: 398 274 147 411 4900
Fragments arranged by size:
4900 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5905
Fragments arranged by size:
5905 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2450 1186
Size: 1264 4866
BseYI C'CCAG_C
Cuts at: 2295 2925 3031 5333 5829 2295
Size: 630 106 2302 496 2596
Fragments arranged by size:
2596 2302 630 496 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3014 1163
Size: 573 1278 4279
Fragments arranged by size:
4279 1278 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3373 3580 4341 5369 1540
Size: 275 186 1372 207 761 1028 2301
Fragments arranged by size:
2301 1372 1028 761 275 207 186
BsiHKAI G_wGCw'C
Cuts at: 2790 3146 3351 4191 4276 5347 2790
Size: 356 205 840 85 1071 3573
Fragments arranged by size:
3573 1071 840 356 205 85
BsmI GAATG_Cn'
Cuts at: 3641 3734 3641
Size: 93 6037
BsmBI CGTCTCn'nnnn_
Cuts at: 2346 2581 2346
Size: 235 5895
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2790 3146 3351 3449 4191 4276 5347
Size: 399 1000 356 205 98 742 85 1071
Cuts at: 5347 1391
Size: 2174
Fragments arranged by size:
2174 1071 1000 742 399 356 205 98
85
BspEI T'CCGG_A
Cuts at: 5793 5793
Size: 6130
BspHI T'CATG_A
Cuts at: 4019 5749 4019
Size: 1730 4400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2478 3291 3485 844
Size: 1634 813 194 3489
Fragments arranged by size:
3489 1634 813 194
BsrBI CCG'CTC
Cuts at: 1394 1823 4017 1394
Size: 429 2194 3507
Fragments arranged by size:
3507 2194 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4625 4799 65
Size: 1183 3377 174 1396
Fragments arranged by size:
3377 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2210 4774 4961 1700
Size: 110 400 2564 187 2869
Fragments arranged by size:
2869 2564 400 187 110
BsrGI T'GTAC_A
Cuts at: 96 2334 96
Size: 2238 3892
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6130
BssSI C'ACGA_G
Cuts at: 4190 5202 4190
Size: 1012 5118
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6130
BstBI TT'CG_AA
Cuts at: 4934 4934
Size: 6130
BstEII G'GTnAC_C
Cuts at: 1184 2314 1184
Size: 1130 5000
BstXI CCAn_nnnn'nTGG
Cuts at: 2547 2812 2818 2547
Size: 265 6 5859
Fragments arranged by size:
5859 265 6
BstYI r'GATC_y
Cuts at: 1972 2535 3460 3827 4212 4229 5681 5767 5779
Size: 563 925 367 385 17 1452 86 12
Cuts at: 5779 6125 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 925 563 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2589 2811 3015 513
Size: 552 939 585 222 204 3628
Fragments arranged by size:
3628 939 585 552 222 204
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5391
Fragments arranged by size:
5391 683 56
BtsI GCAGTG_nn'
Cuts at: 2093 2540 3405 3646 4440 4460 2093
Size: 447 865 241 794 20 3763
Fragments arranged by size:
3763 865 794 447 241 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2069 2104 529
Size: 35 1505 35 4555
Fragments arranged by size:
4555 1505 35 35
DraI TTT'AAA
Cuts at: 3434 3434
Size: 6130
DraIII CAC_nnn'GTG
Cuts at: 2992 3016 2992
Size: 24 6106
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5137 809
Size: 507 3821 1802
Fragments arranged by size:
3821 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3063 3277 3312
Size: 54 1425 50 275 18 1233 214 35
Cuts at: 3312 3370 3577 4466 6095 8
Size: 58 207 889 1629 43
Fragments arranged by size:
1629 1425 1233 889 275 214 207 58
54 50 43 35 18
EagI C'GGCC_G
Cuts at: 1537 1812 3370 3577 1537
Size: 275 1558 207 4090
Fragments arranged by size:
4090 1558 275 207
EarI CTCTTCn'nnn_
Cuts at: 1353 4060 1353
Size: 2707 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3077 3145 4717 5091 5237 387
Size: 1064 138 1488 68 1572 374 146 1280
Fragments arranged by size:
1572 1488 1280 1064 374 146 138 68
EcoICRI GAG'CTC
Cuts at: 2788 2788
Size: 6130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2900 4193 4790 5577 5845 1239
Size: 393 1268 1293 597 787 268 1524
Fragments arranged by size:
1524 1293 1268 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3079 3079
Size: 6130
EcoRI G'AATT_C
Cuts at: 3439 3817 3439
Size: 378 5752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2620 2734 2620
Size: 114 6016
FspI TGC'GCA
Cuts at: 4636 4636
Size: 6130
HaeII r_GCGC'y
Cuts at: 1678 2622 2731 3106 3388 5277 1678
Size: 944 109 375 282 1889 2531
Fragments arranged by size:
2531 1889 944 375 282 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4771 4803 4845 4877 1373
Size: 32 135 32 3199 32 42 32 2626
Fragments arranged by size:
3199 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2824 3474 3720 669
Size: 988 281 886 650 246 3079
Fragments arranged by size:
3079 988 886 650 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6130
HpaI GTT'AAC
Cuts at: 3720 3720
Size: 6130
KasI G'GCGC_C
Cuts at: 2727 2727
Size: 6130
KpnI G_GTAC'C
Cuts at: 2878 2920 3455 2878
Size: 42 535 5553
Fragments arranged by size:
5553 535 42
MfeI C'AATT_G
Cuts at: 3729 3729
Size: 6130
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5244 5428 1305
Size: 3939 184 2007
Fragments arranged by size:
3939 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3065 3314 10
Size: 54 1425 343 1233 249 2826
Fragments arranged by size:
2826 1425 1233 343 249 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2545 2796 2843 3308 4088 4447 4606
Size: 648 1379 251 47 465 780 359 159
Cuts at: 4606 518
Size: 2042
Fragments arranged by size:
2042 1379 780 648 465 359 251 159
47
MspA1I CmG'CkG
Cuts at: 1606 2199 2782 2998 3333 3354 3546 4223 5371
Size: 593 583 216 335 21 192 677 1148
Cuts at: 5371 5616 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 593 583 335 245 216
192 21
NarI GG'CG_CC
Cuts at: 2728 2728
Size: 6130
NcoI C'CATG_G
Cuts at: 513 1065 2004 2589 2811 513
Size: 552 939 585 222 3832
Fragments arranged by size:
3832 939 585 552 222
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6130
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6130
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 2243 3488 5033 1132
Size: 97 1014 1245 1545 2229
Fragments arranged by size:
2229 1545 1245 1014 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6130
PciI A'CATG_T
Cuts at: 5029 5029
Size: 6130
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6130
PfoI T'CCnGG_A
Cuts at: 2391 3339 2391
Size: 948 5182
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2062 2094 4199 4231 5712 5744 2062
Size: 32 2105 32 1481 32 2448
Fragments arranged by size:
2448 2105 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3079 3079
Size: 6130
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6130
PsiI TTA'TAA
Cuts at: 3700 3700
Size: 6130
PstI C_TGCA'G
Cuts at: 830 2416 2835 2967 3482 830
Size: 1586 419 132 515 3478
Fragments arranged by size:
3478 1586 515 419 132
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6130
PvuII CAG'CTG
Cuts at: 2199 2998 2199
Size: 799 5331
SacI G_AGCT'C
Cuts at: 2790 2790
Size: 6130
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SbfI CC_TGCA'GG
Cuts at: 2416 2835 3482 2416
Size: 419 647 5064
Fragments arranged by size:
5064 647 419
ScaI AGT'ACT
Cuts at: 4378 6090 4378
Size: 1712 4418
SexAI A'CCwGG_T
Cuts at: 2870 2870
Size: 6130
SfcI C'TryA_G
Cuts at: 826 1045 2412 2831 2963 3478 4613 5294 5485
Size: 219 1367 419 132 515 1135 681 191
Cuts at: 5485 826
Size: 1471
Fragments arranged by size:
1471 1367 1135 681 515 419 219 191
132
SfoI GGC'GCC
Cuts at: 2729 2729
Size: 6130
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6130
SmaI CCC'GGG
Cuts at: 1872 2734 3457 1872
Size: 862 723 4545
Fragments arranged by size:
4545 862 723
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2079 4234 5135 5397 820
Size: 197 926 136 2155 901 262 1553
Fragments arranged by size:
2155 1553 926 901 262 197 136
SnaBI TAC'GTA
Cuts at: 493 2380 3921 493
Size: 1887 1541 2702
Fragments arranged by size:
2702 1887 1541
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 3488 1132
Size: 2356 3774
SspI AAT'ATT
Cuts at: 5 52 4054 5
Size: 47 4002 2081
Fragments arranged by size:
4002 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6130
StyI C'CwwG_G
Cuts at: 513 1065 2004 2563 2589 2811 3129 3569 513
Size: 552 939 559 26 222 318 440 3074
Fragments arranged by size:
3074 939 559 552 440 318 222 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4157 4174 4327 4512 4957 325
Size: 1255 2577 17 153 185 445 1498
Fragments arranged by size:
2577 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2193 2334 2973
Size: 274 80 33 51 1443 216 141 639
Cuts at: 2973 4376 6088 96
Size: 1403 1712 138
Fragments arranged by size:
1712 1443 1403 639 274 216 141 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6090 6122 6090
Size: 32 6098
Tth111I GACn'n_nGTC
Cuts at: 1316 2228 2936 1316
Size: 912 708 4510
Fragments arranged by size:
4510 912 708
XbaI T'CTAG_A
Cuts at: 2047 3466 2047
Size: 1419 4711
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2587 2587
Size: 6130
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6130
XmaI C'CCGG_G
Cuts at: 1870 2732 3455 1870
Size: 862 723 4545
Fragments arranged by size:
4545 862 723
XmnI GAAnn'nnTTC
Cuts at: 4259 4259
Size: 6130
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3938 276
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI