Restriction map for
pFN21AE4448
(Circular) (Six-base) MAPSORT of: pFN21AE4448.seq Check: 366 from: 1 to: 6094
pFN21AE4448 6094 bp
With 182 enzymes: SgfI *
March 28, 2012 14:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3904 278
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3158 3437 1937
Size: 1221 279 4594
Fragments arranged by size:
4594 1221 279
Acc65I G'GTAC_C
Cuts at: 2991 3359 3415 2991
Size: 368 56 5670
Fragments arranged by size:
5670 368 56
AclI AA'CG_TT
Cuts at: 1563 4221 4594 1563
Size: 2658 373 3063
Fragments arranged by size:
3063 2658 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2378 4157 5541 5809 1632
Size: 746 1779 1384 268 1917
Fragments arranged by size:
1917 1779 1384 746 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5897
AflIII A'CryG_T
Cuts at: 1203 2537 3330 4993 5970 1203
Size: 1334 793 1663 977 1327
Fragments arranged by size:
1663 1334 1327 977 793
AgeI A'CCGG_T
Cuts at: 4925 4925
Size: 6094
AhdI GACnn_n'nnGTC
Cuts at: 2428 3316 4823 2428
Size: 888 1507 3699
Fragments arranged by size:
3699 1507 888
AleI CACnn'nnGTG
Cuts at: 1166 2231 1166
Size: 1065 5029
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6094
AlwNI CAG_nnn'CTG
Cuts at: 2056 3025 3837 5409 2056
Size: 969 812 1572 2741
Fragments arranged by size:
2741 1572 969 812
ApaI G_GGCC'C
Cuts at: 2262 2262
Size: 6094
ApaLI G'TGCA_C
Cuts at: 2699 4151 5307 2699
Size: 1452 1156 3486
Fragments arranged by size:
3486 1452 1156
ApoI r'AATT_y
Cuts at: 1493 3403 3629 3781 5890 1493
Size: 1910 226 152 2109 1697
Fragments arranged by size:
2109 1910 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4648 160
Size: 872 3616 1606
Fragments arranged by size:
3616 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
AvaI C'yCGr_G
Cuts at: 1870 1943 2499 2970 3419 1870
Size: 73 556 471 449 4545
Fragments arranged by size:
4545 556 471 449 73
BamHI G'GATC_C
Cuts at: 2511 3424 3791 2511
Size: 913 367 4814
Fragments arranged by size:
4814 913 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2991 3359 3415 4870 618
Size: 325 217 627 1204 368 56 1455 1842
Fragments arranged by size:
1842 1455 1204 627 368 325 217 56
BanII G_rGCy'C
Cuts at: 1391 2262 2349 2665 2891 3413 1391
Size: 871 87 316 226 522 4072
Fragments arranged by size:
4072 871 522 316 226 87
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2921 3125 928
Size: 971 1022 204 3897
Fragments arranged by size:
3897 1022 971 204
BbvCI CC'TCA_GC
Cuts at: 2015 2015
Size: 6094
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2961 2995 4285 4319 1903
Size: 34 1024 34 1290 34 3678
Fragments arranged by size:
3678 1290 1024 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3986 5196 3986
Size: 1210 4884
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6094
BfrBI ATG'CAT
Cuts at: 2671 2671
Size: 6094
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3206 3299
Size: 107 122 71 631 605 337 1197 93
Cuts at: 3299 4705 136
Size: 1406 1525
Fragments arranged by size:
1525 1406 1197 631 605 337 122 107
93 71
BglII A'GATC_T
Cuts at: 6089 6089
Size: 6094
BlpI GC'TnA_GC
Cuts at: 3511 3511
Size: 6094
Bme1580I G_kGCm'C
Cuts at: 1790 2137 2262 2506 2703 4155 5311 1790
Size: 347 125 244 197 1452 1156 2573
Fragments arranged by size:
2573 1452 1156 347 244 197 125
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6094
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3825 4783 442
Size: 416 53 83 399 24 2408 958 1753
Fragments arranged by size:
2408 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2091 2679 3165 4754 1239
Size: 852 588 486 1589 2579
Fragments arranged by size:
2579 1589 852 588 486
Bpu10I CC'TnA_GC
Cuts at: 2015 2890 3102 2015
Size: 875 212 5007
Fragments arranged by size:
5007 875 212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4219 5084 5382 4219
Size: 865 298 4931
Fragments arranged by size:
4931 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4757 882
Size: 617 3258 2219
Fragments arranged by size:
3258 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3885 493
Size: 711 2681 2702
Fragments arranged by size:
2702 2681 711
BsaBI GATnn'nnATC
Cuts at: 6088 6088
Size: 6094
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 3901 4283 4918
Size: 53 83 186 936 507 1861 382 635
Cuts at: 4918 275
Size: 1451
Fragments arranged by size:
1861 1451 936 635 507 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4527 4925 5199 5346 5757 4527
Size: 398 274 147 411 4864
Fragments arranged by size:
4864 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2618 2648 1178
Size: 30 165 30 1215 30 4624
Fragments arranged by size:
4624 1215 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2484 2487 2709 2795 3023 1186
Size: 1298 3 222 86 228 4257
Fragments arranged by size:
4257 1298 228 222 86 3
BseYI C'CCAG_C
Cuts at: 2093 2269 2919 5297 5793 2093
Size: 176 650 2378 496 2394
Fragments arranged by size:
2394 2378 650 496 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2336 1163
Size: 573 600 4921
Fragments arranged by size:
4921 600 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3544 4305 5333 1540
Size: 275 186 1543 761 1028 2301
Fragments arranged by size:
2301 1543 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2703 4155 4240 5311 2703
Size: 1452 85 1071 3486
Fragments arranged by size:
3486 1452 1071 85
BsmI GAATG_Cn'
Cuts at: 2673 3605 3698 2673
Size: 932 93 5069
Fragments arranged by size:
5069 932 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2137 2262 2349 2506 2665 2703 2891
Size: 399 347 125 87 157 159 38 188
Cuts at: 2891 3413 4155 4240 5311 1391
Size: 522 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 522 399 347 188 159
157 125 87 85 38
BspEI T'CCGG_A
Cuts at: 5757 5757
Size: 6094
BspHI T'CATG_A
Cuts at: 3983 5713 3983
Size: 1730 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2916 3449 844
Size: 2072 533 3489
Fragments arranged by size:
3489 2072 533
BsrBI CCG'CTC
Cuts at: 1394 1823 3981 1394
Size: 429 2158 3507
Fragments arranged by size:
3507 2158 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4589 4763 65
Size: 1183 3341 174 1396
Fragments arranged by size:
3341 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2415 4738 4925 1700
Size: 110 605 2323 187 2869
Fragments arranged by size:
2869 2323 605 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6094
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6094
BssSI C'ACGA_G
Cuts at: 4154 5166 4154
Size: 1012 5082
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6094
BstBI TT'CG_AA
Cuts at: 4898 4898
Size: 6094
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6094
BstXI CCAn_nnnn'nTGG
Cuts at: 3302 3302
Size: 6094
BstYI r'GATC_y
Cuts at: 1972 2511 2802 3424 3791 4176 4193 5645 5731
Size: 539 291 622 367 385 17 1452 86
Cuts at: 5731 5743 6089 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 622 539 385 367 346 291
86 17 12
BstZ17I GTA'TAC
Cuts at: 3159 3159
Size: 6094
BtgI C'CryG_G
Cuts at: 513 1065 2004 3126 513
Size: 552 939 1122 3481
Fragments arranged by size:
3481 1122 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5355
Fragments arranged by size:
5355 683 56
BtsI GCAGTG_nn'
Cuts at: 2117 3610 4404 4424 2117
Size: 1493 794 20 3787
Fragments arranged by size:
3787 1493 794 20
ClaI AT'CG_AT
Cuts at: 1554 3265 1554
Size: 1711 4383
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6059
DraI TTT'AAA
Cuts at: 3398 3398
Size: 6094
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5101 809
Size: 507 3785 1802
Fragments arranged by size:
3785 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2687 3541 4430
Size: 54 1425 50 275 18 857 854 889
Cuts at: 4430 6059 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 857 854 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3541 1537
Size: 275 1729 4090
Fragments arranged by size:
4090 1729 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2766 4024 1353
Size: 1413 1258 3423
Fragments arranged by size:
3423 1413 1258
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4681 5055 5201 387
Size: 1064 138 3092 374 146 1280
Fragments arranged by size:
3092 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2091 2378 2679 3165 4157 4754 5541
Size: 393 459 287 301 486 992 597 787
Cuts at: 5541 5809 1239
Size: 268 1524
Fragments arranged by size:
1524 992 787 597 486 459 393 301
287 268
EcoO109I rG'GnC_Cy
Cuts at: 2259 2398 2707 2905 2259
Size: 139 309 198 5448
Fragments arranged by size:
5448 309 198 139
EcoRI G'AATT_C
Cuts at: 3403 3781 3403
Size: 378 5716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2870 2870
Size: 6094
FspI TGC'GCA
Cuts at: 4600 4600
Size: 6094
HaeII r_GCGC'y
Cuts at: 1678 2015 5241 1678
Size: 337 3226 2531
Fragments arranged by size:
3226 2531 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2414 2446 2639 2671 3258
Size: 32 135 32 842 32 193 32 587
Cuts at: 3258 3265 3290 3297 3302 3334 4735 4767 4809
Size: 7 25 7 5 32 1401 32 42
Cuts at: 4809 4841 1373
Size: 32 2626
Fragments arranged by size:
2626 1401 842 587 193 135 42 32
32 32 32 32 32 32 25 7
7 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3438 3684 669
Size: 988 281 1500 246 3079
Fragments arranged by size:
3079 1500 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6094
HpaI GTT'AAC
Cuts at: 3684 3684
Size: 6094
KpnI G_GTAC'C
Cuts at: 2995 3363 3419 2995
Size: 368 56 5670
Fragments arranged by size:
5670 368 56
MfeI C'AATT_G
Cuts at: 2229 2820 3693 2229
Size: 591 873 4630
Fragments arranged by size:
4630 873 591
MluI A'CGCG_T
Cuts at: 5970 5970
Size: 6094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2757 5208 5392 1305
Size: 1452 2451 184 2007
Fragments arranged by size:
2451 2007 1452 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4272
Fragments arranged by size:
4272 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 2561 4052 4411 4570 518
Size: 648 1065 330 1491 359 159 2042
Fragments arranged by size:
2042 1491 1065 648 359 330 159
MspA1I CmG'CkG
Cuts at: 1606 3510 4187 5335 5580 1606
Size: 1904 677 1148 245 2120
Fragments arranged by size:
2120 1904 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3126 513
Size: 552 939 1122 3481
Fragments arranged by size:
3481 1122 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6094
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6094
NotI GC'GGCC_GC
Cuts at: 3541 3541
Size: 6094
NsiI A_TGCA'T
Cuts at: 2673 2673
Size: 6094
NspI r_CATG'y
Cuts at: 1132 1229 2576 2675 3334 3452 4997 1132
Size: 97 1347 99 659 118 1545 2229
Fragments arranged by size:
2229 1545 1347 659 118 99 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6094
PciI A'CATG_T
Cuts at: 3330 4993 3330
Size: 1663 4431
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2557 1108
Size: 1449 4645
PfoI T'CCnGG_A
Cuts at: 3181 3181
Size: 6094
PmeI GTTT'AAAC
Cuts at: 3398 3398
Size: 6094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4163 4195 5676 5708 4163
Size: 32 1481 32 4549
Fragments arranged by size:
4549 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2707 2905 2707
Size: 198 5896
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6094
PsiI TTA'TAA
Cuts at: 3664 3664
Size: 6094
PspOMI G'GGCC_C
Cuts at: 2258 2258
Size: 6094
PstI C_TGCA'G
Cuts at: 830 3446 830
Size: 2616 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6094
SalI G'TCGA_C
Cuts at: 1936 3436 1936
Size: 1500 4594
SbfI CC_TGCA'GG
Cuts at: 3446 3446
Size: 6094
ScaI AGT'ACT
Cuts at: 4342 6054 4342
Size: 1712 4382
SexAI A'CCwGG_T
Cuts at: 2940 3234 2940
Size: 294 5800
SfcI C'TryA_G
Cuts at: 826 1045 3442 4577 5258 5449 826
Size: 219 2397 1135 681 191 1471
Fragments arranged by size:
2397 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6094
SmaI CCC'GGG
Cuts at: 1872 3421 1872
Size: 1549 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2970 4198 5099 5361 820
Size: 197 926 1027 1228 901 262 1553
Fragments arranged by size:
1553 1228 1027 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3885 493
Size: 3392 2702
SpeI A'CTAG_T
Cuts at: 152 2293 152
Size: 2141 3953
SphI G_CATG'C
Cuts at: 1132 2675 3452 1132
Size: 1543 777 3774
Fragments arranged by size:
3774 1543 777
SspI AAT'ATT
Cuts at: 5 52 4018 5
Size: 47 3966 2081
Fragments arranged by size:
3966 2081 47
StuI AGG'CCT
Cuts at: 1517 2453 1517
Size: 936 5158
StyI C'CwwG_G
Cuts at: 513 1065 2004 2198 2584 2716 3126 3213 3533
Size: 552 939 194 386 132 410 87 320
Cuts at: 3533 513
Size: 3074
Fragments arranged by size:
3074 939 552 410 386 320 194 132
87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2283 4121 4138 4291 4476 4921 325
Size: 1255 703 1838 17 153 185 445 1498
Fragments arranged by size:
1838 1498 1255 703 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4340 6052 96
Size: 274 80 33 51 1443 2363 1712 138
Fragments arranged by size:
2363 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2496 2528 6054 6086 2496
Size: 32 3526 32 2504
Fragments arranged by size:
3526 2504 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6094
XbaI T'CTAG_A
Cuts at: 3430 3430
Size: 6094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2127 2575 2127
Size: 448 5646
XhoI C'TCGA_G
Cuts at: 1943 2970 1943
Size: 1027 5067
XmaI C'CCGG_G
Cuts at: 1870 3419 1870
Size: 1549 4545
XmnI GAAnn'nnTTC
Cuts at: 4223 4223
Size: 6094
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3902 276
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI