Restriction map for
pFN21AE4446
(Circular) (Six-base) MAPSORT of: pFN21AE4446.seq Check: 280 from: 1 to: 6088
pFN21AE4446 6088 bp
With 182 enzymes: SgfI *
October 12, 2018 18:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3431 1937
Size: 1494 4594
Acc65I G'GTAC_C
Cuts at: 3409 3409
Size: 6088
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4151 5535 5803 1632
Size: 2519 1384 268 1917
Fragments arranged by size:
2519 1917 1384 268
AfeI AGC'GCT
Cuts at: 3110 3110
Size: 6088
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 3007 3080 4987 5964 1203
Size: 1804 73 1907 977 1327
Fragments arranged by size:
1907 1804 1327 977 73
AgeI A'CCGG_T
Cuts at: 4919 4919
Size: 6088
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2711 1184
Size: 1527 4561
AlwNI CAG_nnn'CTG
Cuts at: 2305 2804 3239 3831 5403 2305
Size: 499 435 592 1572 2990
Fragments arranged by size:
2990 1572 592 499 435
ApaLI G'TGCA_C
Cuts at: 4145 5301 4145
Size: 1156 4932
ApoI r'AATT_y
Cuts at: 1493 2510 2744 3397 3623 3775 5884 1493
Size: 1017 234 653 226 152 2109 1697
Fragments arranged by size:
2109 1697 1017 653 234 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2088 3381 4642 160
Size: 872 1056 1293 1261 1606
Fragments arranged by size:
1606 1293 1261 1056 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 3413 1870
Size: 73 1470 4545
Fragments arranged by size:
4545 1470 73
BamHI G'GATC_C
Cuts at: 3418 3785 3418
Size: 367 5721
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2311 2516 3409 4864 618
Size: 325 217 627 524 205 893 1455 1842
Fragments arranged by size:
1842 1455 893 627 524 325 217 205
BanII G_rGCy'C
Cuts at: 1391 2829 2912 3407 1391
Size: 1438 83 495 4072
Fragments arranged by size:
4072 1438 495 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2243 928
Size: 971 344 4773
Fragments arranged by size:
4773 971 344
BbvCI CC'TCA_GC
Cuts at: 2673 2673
Size: 6088
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2212 2246 4279 4313 1903
Size: 34 275 34 2033 34 3678
Fragments arranged by size:
3678 2033 275 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3980 5190 3980
Size: 1210 4878
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6088
BfrBI ATG'CAT
Cuts at: 3130 3130
Size: 6088
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3244 4699 136
Size: 107 122 71 631 605 1572 1455 1525
Fragments arranged by size:
1572 1525 1455 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6083 6083
Size: 6088
BlpI GC'TnA_GC
Cuts at: 2823 3505 2823
Size: 682 5406
Bme1580I G_kGCm'C
Cuts at: 1790 4149 5305 1790
Size: 2359 1156 2573
Fragments arranged by size:
2573 2359 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6088
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3819 4777 442
Size: 416 53 83 399 24 2402 958 1753
Fragments arranged by size:
2402 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2277 1056
Size: 1221 4867
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2301 2826 4748 1239
Size: 1062 525 1922 2579
Fragments arranged by size:
2579 1922 1062 525
Bpu10I CC'TnA_GC
Cuts at: 2673 2673
Size: 6088
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4213 5078 5376 4213
Size: 865 298 4925
Fragments arranged by size:
4925 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2615 3025 4751 882
Size: 617 1116 410 1726 2219
Fragments arranged by size:
2219 1726 1116 617 410
BsaAI yAC'GTr
Cuts at: 493 1204 3879 493
Size: 711 2675 2702
Fragments arranged by size:
2702 2675 711
BsaBI GATnn'nnATC
Cuts at: 6082 6082
Size: 6088
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2967 3895 4277 4912
Size: 53 83 186 936 1434 928 382 635
Cuts at: 4912 275
Size: 1451
Fragments arranged by size:
1451 1434 936 928 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4521 4919 5193 5340 5751 4521
Size: 398 274 147 411 4858
Fragments arranged by size:
4858 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5863
Fragments arranged by size:
5863 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6088
BseYI C'CCAG_C
Cuts at: 2773 5291 5787 2773
Size: 2518 496 3074
Fragments arranged by size:
3074 2518 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2318 2651 1163
Size: 573 582 333 4600
Fragments arranged by size:
4600 582 573 333
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3097 3538 4299 5327 1540
Size: 275 186 1096 441 761 1028 2301
Fragments arranged by size:
2301 1096 1028 761 441 275 186
BsiHKAI G_wGCw'C
Cuts at: 2440 4149 4234 5305 2440
Size: 1709 85 1071 3223
Fragments arranged by size:
3223 1709 1071 85
BsmI GAATG_Cn'
Cuts at: 3599 3692 3599
Size: 93 5995
BsmBI CGTCTCn'nnnn_
Cuts at: 2504 2504
Size: 6088
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2440 2829 2912 3407 4149 4234 5305
Size: 399 650 389 83 495 742 85 1071
Cuts at: 5305 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 650 495 399 389 85
83
BspEI T'CCGG_A
Cuts at: 5751 5751
Size: 6088
BspHI T'CATG_A
Cuts at: 3977 5707 3977
Size: 1730 4358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3126 3443 844
Size: 2282 317 3489
Fragments arranged by size:
3489 2282 317
BsrBI CCG'CTC
Cuts at: 1394 1823 3975 1394
Size: 429 2152 3507
Fragments arranged by size:
3507 2152 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2874 4583 4757 65
Size: 1183 1626 1709 174 1396
Fragments arranged by size:
1709 1626 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2759 4732 4919 1700
Size: 110 949 1973 187 2869
Fragments arranged by size:
2869 1973 949 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6088
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6088
BssSI C'ACGA_G
Cuts at: 2184 4148 5160 2184
Size: 1964 1012 3112
Fragments arranged by size:
3112 1964 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3253 3365 794
Size: 2459 112 3517
Fragments arranged by size:
3517 2459 112
BstBI TT'CG_AA
Cuts at: 4892 4892
Size: 6088
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 2723 3418 3785 4170 4187 5639 5725 5737
Size: 751 695 367 385 17 1452 86 12
Cuts at: 5737 6083 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 751 695 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5536
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5349
Fragments arranged by size:
5349 683 56
BtsI GCAGTG_nn'
Cuts at: 3604 4398 4418 3604
Size: 794 20 5274
Fragments arranged by size:
5274 794 20
ClaI AT'CG_AT
Cuts at: 1554 2571 1554
Size: 1017 5071
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6053
DraI TTT'AAA
Cuts at: 3392 3392
Size: 6088
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5095 809
Size: 507 3779 1802
Fragments arranged by size:
3779 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3535 4424 6053
Size: 54 1425 50 275 18 1705 889 1629
Cuts at: 6053 8
Size: 43
Fragments arranged by size:
1705 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3535 1537
Size: 275 1723 4090
Fragments arranged by size:
4090 1723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2019 2745 4018 1353
Size: 666 726 1273 3423
Fragments arranged by size:
3423 1273 726 666
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4675 5049 5195 387
Size: 1064 138 3086 374 146 1280
Fragments arranged by size:
3086 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2301 2826 4151 4748 5535 5803 1239
Size: 393 669 525 1325 597 787 268 1524
Fragments arranged by size:
1524 1325 787 669 597 525 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3236 3236
Size: 6088
EcoRI G'AATT_C
Cuts at: 3397 3775 3397
Size: 378 5710
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 6088
FspI TGC'GCA
Cuts at: 4594 4594
Size: 6088
HaeII r_GCGC'y
Cuts at: 1678 3112 3168 5235 1678
Size: 1434 56 2067 2531
Fragments arranged by size:
2531 2067 1434 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2799 2831 4729 4761 4803
Size: 32 135 32 1227 32 1898 32 42
Cuts at: 4803 4835 1373
Size: 32 2626
Fragments arranged by size:
2626 1898 1227 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3432 3678 669
Size: 988 281 1494 246 3079
Fragments arranged by size:
3079 1494 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6088
HpaI GTT'AAC
Cuts at: 3678 3678
Size: 6088
KpnI G_GTAC'C
Cuts at: 3413 3413
Size: 6088
MfeI C'AATT_G
Cuts at: 3687 3687
Size: 6088
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2760 5202 5386 1305
Size: 1455 2442 184 2007
Fragments arranged by size:
2442 2007 1455 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4266
Fragments arranged by size:
4266 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3006 3135 4046 4405 4564 518
Size: 648 1840 129 911 359 159 2042
Fragments arranged by size:
2042 1840 911 648 359 159 129
MspA1I CmG'CkG
Cuts at: 1606 2652 3504 4181 5329 5574 1606
Size: 1046 852 677 1148 245 2120
Fragments arranged by size:
2120 1148 1046 852 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5536
NdeI CA'TA_TG
Cuts at: 387 3068 387
Size: 2681 3407
NheI G'CTAG_C
Cuts at: 1052 2273 1052
Size: 1221 4867
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NsiI A_TGCA'T
Cuts at: 3132 3132
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 2692 3011 3084 3134 3446 4991 1132
Size: 97 1463 319 73 50 312 1545 2229
Fragments arranged by size:
2229 1545 1463 319 312 97 73 50
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6088
PciI A'CATG_T
Cuts at: 3007 3080 4987 3007
Size: 73 1907 4108
Fragments arranged by size:
4108 1907 73
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3069 1108
Size: 1961 4127
PfoI T'CCnGG_A
Cuts at: 2719 2719
Size: 6088
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6088
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PstI C_TGCA'G
Cuts at: 830 3440 830
Size: 2610 3478
PvuI CG_AT'CG
Cuts at: 2001 3097 2001
Size: 1096 4992
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SbfI CC_TGCA'GG
Cuts at: 3440 3440
Size: 6088
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SfcI C'TryA_G
Cuts at: 826 1045 3436 4571 5252 5443 826
Size: 219 2391 1135 681 191 1471
Fragments arranged by size:
2391 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 2759 1700
Size: 1059 5029
SmaI CCC'GGG
Cuts at: 1872 3415 1872
Size: 1543 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4192 5093 5355 820
Size: 197 926 2249 901 262 1553
Fragments arranged by size:
2249 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3879 493
Size: 3386 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 3446 1132
Size: 2314 3774
SspI AAT'ATT
Cuts at: 5 52 4012 5
Size: 47 3960 2081
Fragments arranged by size:
3960 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6088
StyI C'CwwG_G
Cuts at: 513 1065 2064 3527 513
Size: 552 999 1463 3074
Fragments arranged by size:
3074 1463 999 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3125 4115 4132 4285 4470 4915 325
Size: 1255 1545 990 17 153 185 445 1498
Fragments arranged by size:
1545 1498 1255 990 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4334 6046 96
Size: 274 80 33 51 1443 2357 1712 138
Fragments arranged by size:
2357 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6048 6080 6048
Size: 32 6056
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6088
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2071 2452 2071
Size: 381 5707
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6088
XmaI C'CCGG_G
Cuts at: 1870 3413 1870
Size: 1543 4545
XmnI GAAnn'nnTTC
Cuts at: 4217 4217
Size: 6088
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI