Restriction map for
pFN21AE4445
(Circular) (Six-base) MAPSORT of: pFN21AE4445.seq Check: 1101 from: 1 to: 6088
pFN21AE4445 6088 bp
With 182 enzymes: SgfI *
March 28, 2012 13:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3152 3431 1937
Size: 1215 279 4594
Fragments arranged by size:
4594 1215 279
Acc65I G'GTAC_C
Cuts at: 3409 3409
Size: 6088
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2275 3299 4151 5535 5803 1632
Size: 643 1024 852 1384 268 1917
Fragments arranged by size:
1917 1384 1024 852 643 268
AfeI AGC'GCT
Cuts at: 2261 2261
Size: 6088
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 4987 5964 1203
Size: 3784 977 1327
Fragments arranged by size:
3784 1327 977
AgeI A'CCGG_T
Cuts at: 4919 4919
Size: 6088
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2337 1184
Size: 1153 4935
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2435 2467 3015 3047 2435
Size: 32 548 32 5476
Fragments arranged by size:
5476 548 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3831 5403 3831
Size: 1572 4516
ApaI G_GGCC'C
Cuts at: 2083 2083
Size: 6088
ApaLI G'TGCA_C
Cuts at: 2875 4145 5301 2875
Size: 1270 1156 3662
Fragments arranged by size:
3662 1270 1156
ApoI r'AATT_y
Cuts at: 1493 2719 2834 3264 3397 3623 3775 5884 1493
Size: 1226 115 430 133 226 152 2109 1697
Fragments arranged by size:
2109 1697 1226 430 226 152 133 115
AseI AT'TA_AT
Cuts at: 160 1032 4642 160
Size: 872 3610 1606
Fragments arranged by size:
3610 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 3413 1870
Size: 73 1470 4545
Fragments arranged by size:
4545 1470 73
BamHI G'GATC_C
Cuts at: 3418 3785 3418
Size: 367 5721
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2194 2372 3409 4864 618
Size: 325 217 627 407 178 1037 1455 1842
Fragments arranged by size:
1842 1455 1037 627 407 325 217 178
BanII G_rGCy'C
Cuts at: 1391 2083 3407 1391
Size: 692 1324 4072
Fragments arranged by size:
4072 1324 692
BbeI G_GCGC'C
Cuts at: 2376 2376
Size: 6088
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5117
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4279 4313 1903
Size: 34 2342 34 3678
Fragments arranged by size:
3678 2342 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2807 3980 5190 2807
Size: 1173 1210 3705
Fragments arranged by size:
3705 1210 1173
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6088
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4699 136
Size: 107 122 71 631 605 3027 1525
Fragments arranged by size:
3027 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3274 6083 3274
Size: 2809 3279
BlpI GC'TnA_GC
Cuts at: 3074 3505 3074
Size: 431 5657
Bme1580I G_kGCm'C
Cuts at: 1790 2083 2199 2879 4149 5305 1790
Size: 293 116 680 1270 1156 2573
Fragments arranged by size:
2573 1270 1156 680 293 116
BmgBI CAC'GTC
Cuts at: 1318 2461 1318
Size: 1143 4945
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2188 3319 3819
Size: 416 53 83 399 24 771 1131 500
Cuts at: 3819 4777 442
Size: 958 1753
Fragments arranged by size:
1753 1131 958 771 500 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4748 1239
Size: 3509 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4213 5078 5376 4213
Size: 865 298 4925
Fragments arranged by size:
4925 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3311 4751 882
Size: 617 1812 1440 2219
Fragments arranged by size:
2219 1812 1440 617
BsaAI yAC'GTr
Cuts at: 493 1204 3879 493
Size: 711 2675 2702
Fragments arranged by size:
2702 2675 711
BsaBI GATnn'nnATC
Cuts at: 6082 6082
Size: 6088
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2373 3895 4277 4912
Size: 53 83 186 936 840 1522 382 635
Cuts at: 4912 275
Size: 1451
Fragments arranged by size:
1522 1451 936 840 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2505 4521 4919 5193 5340 5751 2505
Size: 2016 398 274 147 411 2842
Fragments arranged by size:
2842 2016 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2435 2465 2696 2726 3372
Size: 30 165 30 1032 30 231 30 646
Cuts at: 3372 3402 1178
Size: 30 3864
Fragments arranged by size:
3864 1032 646 231 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2357 1186
Size: 1171 4917
BseYI C'CCAG_C
Cuts at: 2082 3259 5291 5787 2082
Size: 1177 2032 496 2383
Fragments arranged by size:
2383 2032 1177 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3173 1163
Size: 573 1437 4078
Fragments arranged by size:
4078 1437 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2018 2064 2132 2249 3538 4299
Size: 275 186 17 46 68 117 1289 761
Cuts at: 4299 5327 1540
Size: 1028 2301
Fragments arranged by size:
2301 1289 1028 761 275 186 117 68
46 17
BsiHKAI G_wGCw'C
Cuts at: 2879 4149 4234 5305 2879
Size: 1270 85 1071 3662
Fragments arranged by size:
3662 1270 1071 85
BsmI GAATG_Cn'
Cuts at: 3599 3692 3599
Size: 93 5995
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2199 2879 3407 4149 4234 5305
Size: 399 293 116 680 528 742 85 1071
Cuts at: 5305 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 680 528 399 293 116
85
BspEI T'CCGG_A
Cuts at: 2505 5751 2505
Size: 3246 2842
BspHI T'CATG_A
Cuts at: 3977 5707 3977
Size: 1730 4358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3443 844
Size: 2599 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2060 3975 1394
Size: 429 237 1915 3507
Fragments arranged by size:
3507 1915 429 237
BsrDI GCAATG_nn'
Cuts at: 65 1248 3128 4583 4757 65
Size: 1183 1880 1455 174 1396
Fragments arranged by size:
1880 1455 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2017 2137 4732 4919 1700
Size: 110 207 120 2595 187 2869
Fragments arranged by size:
2869 2595 207 187 120 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6088
BssHII G'CGCG_C
Cuts at: 1925 2324 1925
Size: 399 5689
BssSI C'ACGA_G
Cuts at: 4148 5160 4148
Size: 1012 5076
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3356 794
Size: 2562 3526
BstBI TT'CG_AA
Cuts at: 4892 4892
Size: 6088
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6088
BstXI CCAn_nnnn'nTGG
Cuts at: 2461 2461
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 3274 3292 3418 3785 4170 4187 5639 5725
Size: 1302 18 126 367 385 17 1452 86
Cuts at: 5725 5737 6083 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1302 385 367 346 126 86
18 17 12
BstZ17I GTA'TAC
Cuts at: 3153 3153
Size: 6088
BtgI C'CryG_G
Cuts at: 513 1065 2367 2806 3110 513
Size: 552 1302 439 304 3491
Fragments arranged by size:
3491 1302 552 439 304
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5349
Fragments arranged by size:
5349 683 56
BtsI GCAGTG_nn'
Cuts at: 2319 3604 4398 4418 2319
Size: 1285 794 20 3989
Fragments arranged by size:
3989 1285 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2832 2867 529
Size: 35 2268 35 3750
Fragments arranged by size:
3750 2268 35 35
DraI TTT'AAA
Cuts at: 3392 3392
Size: 6088
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2750 5095 809
Size: 507 1434 2345 1802
Fragments arranged by size:
2345 1802 1434 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2015 2061 2111
Size: 54 1425 50 275 18 185 46 50
Cuts at: 2111 2129 2246 2423 3194 3535 4424 6053 8
Size: 18 117 177 771 341 889 1629 43
Fragments arranged by size:
1629 1425 889 771 341 275 185 177
117 54 50 50 46 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2015 2061 2129 2246 3535 1537
Size: 275 203 46 68 117 1289 4090
Fragments arranged by size:
4090 1289 275 203 117 68 46
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 2363 2504 3314 4018 1353
Size: 701 309 141 810 704 3423
Fragments arranged by size:
3423 810 704 701 309 141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4675 5049 5195 387
Size: 1064 138 3086 374 146 1280
Fragments arranged by size:
3086 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2275 3299 4151 4748 5535 5803 1239
Size: 393 643 1024 852 597 787 268 1524
Fragments arranged by size:
1524 1024 852 787 643 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 2360
Size: 6088
EcoO109I rG'GnC_Cy
Cuts at: 2024 2465 2024
Size: 441 5647
EcoRI G'AATT_C
Cuts at: 3264 3397 3775 3264
Size: 133 378 5577
Fragments arranged by size:
5577 378 133
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3068 3068
Size: 6088
FspI TGC'GCA
Cuts at: 4594 4594
Size: 6088
HaeII r_GCGC'y
Cuts at: 1678 2263 2376 5235 1678
Size: 585 113 2859 2531
Fragments arranged by size:
2859 2531 585 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2386 2418 4729 4761 4803
Size: 32 135 32 814 32 2311 32 42
Cuts at: 4803 4835 1373
Size: 32 2626
Fragments arranged by size:
2626 2311 814 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2647 3432 3678 669
Size: 988 281 709 785 246 3079
Fragments arranged by size:
3079 988 785 709 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6088
HpaI GTT'AAC
Cuts at: 2647 3678 2647
Size: 1031 5057
KasI G'GCGC_C
Cuts at: 2372 2372
Size: 6088
KpnI G_GTAC'C
Cuts at: 3413 3413
Size: 6088
MfeI C'AATT_G
Cuts at: 3687 3687
Size: 6088
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5202 5386 1305
Size: 3897 184 2007
Fragments arranged by size:
3897 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3196 10
Size: 54 1425 343 1364 2902
Fragments arranged by size:
2902 1425 1364 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3145 4046 4405 4564 518
Size: 648 1979 901 359 159 2042
Fragments arranged by size:
2042 1979 901 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2134 2369 3504 4181 5329 5574 1606
Size: 528 235 1135 677 1148 245 2120
Fragments arranged by size:
2120 1148 1135 677 528 245 235
NaeI GCC'GGC
Cuts at: 2019 2139 2019
Size: 120 5968
NarI GG'CG_CC
Cuts at: 2373 2373
Size: 6088
NcoI C'CATG_G
Cuts at: 513 1065 2806 3110 513
Size: 552 1741 304 3491
Fragments arranged by size:
3491 1741 552 304
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NgoMIV G'CCGG_C
Cuts at: 2017 2137 2017
Size: 120 5968
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6088
NotI GC'GGCC_GC
Cuts at: 2246 3535 2246
Size: 1289 4799
NspI r_CATG'y
Cuts at: 1132 1229 3446 4991 1132
Size: 97 2217 1545 2229
Fragments arranged by size:
2229 2217 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6088
PciI A'CATG_T
Cuts at: 4987 4987
Size: 6088
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3111 3203 1108
Size: 2003 92 3993
Fragments arranged by size:
3993 2003 92
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2435 2467 4157 4189 5670 5702 2435
Size: 32 1690 32 1481 32 2821
Fragments arranged by size:
2821 1690 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2024 2024
Size: 6088
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6088
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PspOMI G'GGCC_C
Cuts at: 2079 2079
Size: 6088
PstI C_TGCA'G
Cuts at: 830 3440 830
Size: 2610 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
RsrII CG'GwC_CG
Cuts at: 2309 2309
Size: 6088
SacII CC_GC'GG
Cuts at: 2370 2370
Size: 6088
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SanDI GG'GwC_CC
Cuts at: 2024 2024
Size: 6088
SapI GCTCTTCn'nnn_
Cuts at: 2054 2054
Size: 6088
SbfI CC_TGCA'GG
Cuts at: 3440 3440
Size: 6088
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SfcI C'TryA_G
Cuts at: 826 1045 3436 4571 5252 5443 826
Size: 219 2391 1135 681 191 1471
Fragments arranged by size:
2391 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2374 2374
Size: 6088
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6088
SmaI CCC'GGG
Cuts at: 1872 3415 1872
Size: 1543 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4192 5093 5355 820
Size: 197 926 2249 901 262 1553
Fragments arranged by size:
2249 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3879 493
Size: 3386 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 3446 1132
Size: 2314 3774
SspI AAT'ATT
Cuts at: 5 52 4012 5
Size: 47 3960 2081
Fragments arranged by size:
3960 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6088
StyI C'CwwG_G
Cuts at: 513 1065 2806 3110 3527 513
Size: 552 1741 304 417 3074
Fragments arranged by size:
3074 1741 552 417 304
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2793 4115 4132 4285 4470 4915 325
Size: 1255 1213 1322 17 153 185 445 1498
Fragments arranged by size:
1498 1322 1255 1213 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4334 6046 96
Size: 274 80 33 51 1443 2357 1712 138
Fragments arranged by size:
2357 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6048 6080 6048
Size: 32 6056
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6088
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2862 2862
Size: 6088
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6088
XmaI C'CCGG_G
Cuts at: 1870 3413 1870
Size: 1543 4545
XmnI GAAnn'nnTTC
Cuts at: 2964 3051 4217 2964
Size: 87 1166 4835
Fragments arranged by size:
4835 1166 87
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FseI FspAI
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
SacI SexAI SfiI SrfI SwaI