(Circular) (Six-base) MAPSORT of: pFN21AE4443.seq Check: 2075 from: 1 to: 6082 pFN21AE4443 6082 bp With 182 enzymes: SgfI * March 28, 2012 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3892 278 Size: 53 83 186 936 2356 2468 Fragments arranged by size: 2468 2356 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3034 3425 1937 Size: 1097 391 4594 Fragments arranged by size: 4594 1097 391 Acc65I G'GTAC_C Cuts at: 3245 3403 3245 Size: 158 5924 AclI AA'CG_TT Cuts at: 1563 4209 4582 1563 Size: 2646 373 3063 Fragments arranged by size: 3063 2646 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2502 3152 4145 5529 5797 1632 Size: 870 650 993 1384 268 1917 Fragments arranged by size: 1917 1384 993 870 650 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5885 AflIII A'CryG_T Cuts at: 1203 4981 5958 1203 Size: 3778 977 1327 Fragments arranged by size: 3778 1327 977 AgeI A'CCGG_T Cuts at: 4913 4913 Size: 6082 AhdI GACnn_n'nnGTC Cuts at: 4811 4811 Size: 6082 AleI CACnn'nnGTG Cuts at: 1166 2564 1166 Size: 1398 4684 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6082 AlwNI CAG_nnn'CTG Cuts at: 3130 3293 3825 5397 3130 Size: 163 532 1572 3815 Fragments arranged by size: 3815 1572 532 163 ApaLI G'TGCA_C Cuts at: 2705 4139 5295 2705 Size: 1434 1156 3492 Fragments arranged by size: 3492 1434 1156 ApoI r'AATT_y Cuts at: 1493 2765 3391 3617 3769 5878 1493 Size: 1272 626 226 152 2109 1697 Fragments arranged by size: 2109 1697 1272 626 226 152 AseI AT'TA_AT Cuts at: 160 1032 4636 160 Size: 872 3604 1606 Fragments arranged by size: 3604 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6082 AvaI C'yCGr_G Cuts at: 1870 1943 3234 3407 1870 Size: 73 1291 173 4545 Fragments arranged by size: 4545 1291 173 73 BamHI G'GATC_C Cuts at: 3412 3779 3412 Size: 367 5715 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3245 3403 4858 618 Size: 325 217 627 1458 158 1455 1842 Fragments arranged by size: 1842 1458 1455 627 325 217 158 BanII G_rGCy'C Cuts at: 1391 2748 3401 1391 Size: 1357 653 4072 Fragments arranged by size: 4072 1357 653 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5111 BbvCI CC'TCA_GC Cuts at: 3095 3095 Size: 6082 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4273 4307 1903 Size: 34 2336 34 3678 Fragments arranged by size: 3678 2336 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3974 5184 3974 Size: 1210 4872 BclI T'GATC_A Cuts at: 1548 2007 2635 1548 Size: 459 628 4995 Fragments arranged by size: 4995 628 459 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2274 4693 136 Size: 107 122 71 631 605 602 2419 1525 Fragments arranged by size: 2419 1525 631 605 602 122 107 71 BglII A'GATC_T Cuts at: 2895 6077 2895 Size: 3182 2900 BlpI GC'TnA_GC Cuts at: 3499 3499 Size: 6082 Bme1580I G_kGCm'C Cuts at: 1790 2233 2709 3078 3175 4143 5299 1790 Size: 443 476 369 97 968 1156 2573 Fragments arranged by size: 2573 1156 968 476 443 369 97 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6082 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2194 3813 4771 Size: 416 53 83 399 24 777 1619 958 Cuts at: 4771 442 Size: 1753 Fragments arranged by size: 1753 1619 958 777 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6082 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2195 4742 1239 Size: 803 153 2547 2579 Fragments arranged by size: 2579 2547 803 153 Bpu10I CC'TnA_GC Cuts at: 2814 3095 2814 Size: 281 5801 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4207 5072 5370 4207 Size: 865 298 4919 Fragments arranged by size: 4919 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4745 882 Size: 617 3246 2219 Fragments arranged by size: 3246 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3113 3873 493 Size: 711 1909 760 2702 Fragments arranged by size: 2702 1909 760 711 BsaBI GATnn'nnATC Cuts at: 6076 6076 Size: 6082 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3889 4271 4906 275 Size: 53 83 186 936 2356 382 635 1451 Fragments arranged by size: 2356 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2463 4515 4913 5187 5334 5745 2463 Size: 2052 398 274 147 411 2800 Fragments arranged by size: 2800 2052 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5857 Fragments arranged by size: 5857 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2885 3335 1186 Size: 1699 450 3933 Fragments arranged by size: 3933 1699 450 BseYI C'CCAG_C Cuts at: 2233 5285 5781 2233 Size: 3052 496 2534 Fragments arranged by size: 3052 2534 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5509 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3532 4293 5321 1540 Size: 275 186 1531 761 1028 2301 Fragments arranged by size: 2301 1531 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2709 4143 4228 5299 2709 Size: 1434 85 1071 3492 Fragments arranged by size: 3492 1434 1071 85 BsmI GAATG_Cn' Cuts at: 3593 3686 3593 Size: 93 5989 BsmBI CGTCTCn'nnnn_ Cuts at: 2655 2655 Size: 6082 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2233 2709 2748 3078 3175 3401 4143 Size: 399 443 476 39 330 97 226 742 Cuts at: 4143 4228 5299 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 476 443 399 330 226 97 85 39 BspEI T'CCGG_A Cuts at: 5745 5745 Size: 6082 BspHI T'CATG_A Cuts at: 2769 3971 5701 2769 Size: 1202 1730 3150 Fragments arranged by size: 3150 1730 1202 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3437 844 Size: 2593 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3969 1394 Size: 429 2146 3507 Fragments arranged by size: 3507 2146 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4577 4751 65 Size: 1183 3329 174 1396 Fragments arranged by size: 3329 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4726 4913 1700 Size: 110 2916 187 2869 Fragments arranged by size: 2916 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6082 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6082 BssSI C'ACGA_G Cuts at: 4142 5154 4142 Size: 1012 5070 BstAPI GCAn_nnn'nTGC Cuts at: 794 3343 794 Size: 2549 3533 BstBI TT'CG_AA Cuts at: 4886 4886 Size: 6082 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6082 BstYI r'GATC_y Cuts at: 1972 2895 3283 3412 3779 4164 4181 5633 5719 Size: 923 388 129 367 385 17 1452 86 Cuts at: 5719 5731 6077 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 923 388 385 367 346 129 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2031 2270 2740 513 Size: 552 966 239 470 3855 Fragments arranged by size: 3855 966 552 470 239 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5343 Fragments arranged by size: 5343 683 56 BtsI GCAGTG_nn' Cuts at: 2529 2707 3076 3598 4392 4412 2529 Size: 178 369 522 794 20 4199 Fragments arranged by size: 4199 794 522 369 178 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6082 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6047 DraI TTT'AAA Cuts at: 3386 3386 Size: 6082 DraIII CAC_nnn'GTG Cuts at: 2607 2607 Size: 6082 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5089 809 Size: 507 3773 1802 Fragments arranged by size: 3773 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2737 2750 3529 Size: 54 1425 50 275 18 907 13 779 Cuts at: 3529 4418 6047 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 907 889 779 275 54 50 43 18 13 EagI C'GGCC_G Cuts at: 1537 1812 3529 1537 Size: 275 1717 4090 Fragments arranged by size: 4090 1717 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2444 2478 2529 4012 1353 Size: 1091 34 51 1483 3423 Fragments arranged by size: 3423 1483 1091 51 34 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3346 4669 5043 5189 387 Size: 1064 138 1757 1323 374 146 1280 Fragments arranged by size: 1757 1323 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2195 2502 3152 4145 4742 5529 Size: 393 410 153 307 650 993 597 787 Cuts at: 5529 5797 1239 Size: 268 1524 Fragments arranged by size: 1524 993 787 650 597 410 393 307 268 153 EcoNI CCTnn'n_nnAGG Cuts at: 2813 2813 Size: 6082 EcoO109I rG'GnC_Cy Cuts at: 2121 2142 2149 2259 2776 3127 2121 Size: 21 7 110 517 351 5076 Fragments arranged by size: 5076 517 351 110 21 7 EcoRI G'AATT_C Cuts at: 2765 3391 3769 2765 Size: 626 378 5078 Fragments arranged by size: 5078 626 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2389 2389 Size: 6082 FspI TGC'GCA Cuts at: 4588 4588 Size: 6082 HaeII r_GCGC'y Cuts at: 1678 5229 1678 Size: 3551 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2903 2935 4723 4755 4797 Size: 32 135 32 1331 32 1788 32 42 Cuts at: 4797 4829 1373 Size: 32 2626 Fragments arranged by size: 2626 1788 1331 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2596 2794 3035 3426 3672 669 Size: 988 281 658 198 241 391 246 3079 Fragments arranged by size: 3079 988 658 391 281 246 241 198 HindIII A'AGCT_T Cuts at: 748 2279 3299 748 Size: 1531 1020 3531 Fragments arranged by size: 3531 1531 1020 HpaI GTT'AAC Cuts at: 3672 3672 Size: 6082 KpnI G_GTAC'C Cuts at: 3249 3407 3249 Size: 158 5924 MfeI C'AATT_G Cuts at: 3681 3681 Size: 6082 MluI A'CGCG_T Cuts at: 5958 5958 Size: 6082 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5196 5380 1305 Size: 3891 184 2007 Fragments arranged by size: 3891 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4260 Fragments arranged by size: 4260 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2564 4040 4399 4558 518 Size: 648 1398 1476 359 159 2042 Fragments arranged by size: 2042 1476 1398 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2788 3498 4175 5323 5568 1606 Size: 1182 710 677 1148 245 2120 Fragments arranged by size: 2120 1182 1148 710 677 245 NcoI C'CATG_G Cuts at: 513 1065 2270 2740 513 Size: 552 1205 470 3855 Fragments arranged by size: 3855 1205 552 470 NdeI CA'TA_TG Cuts at: 387 387 Size: 6082 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6082 NotI GC'GGCC_GC Cuts at: 3529 3529 Size: 6082 NspI r_CATG'y Cuts at: 1132 1229 2522 3124 3440 4985 1132 Size: 97 1293 602 316 1545 2229 Fragments arranged by size: 2229 1545 1293 602 316 97 PasI CC'CwG_GG Cuts at: 1348 3045 3071 1348 Size: 1697 26 4359 Fragments arranged by size: 4359 1697 26 PciI A'CATG_T Cuts at: 4981 4981 Size: 6082 PflMI CCAn_nnn'nTGG Cuts at: 1108 2582 1108 Size: 1474 4608 PfoI T'CCnGG_A Cuts at: 2019 2130 2865 2019 Size: 111 735 5236 Fragments arranged by size: 5236 735 111 PmeI GTTT'AAAC Cuts at: 3386 3386 Size: 6082 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4151 4183 5664 5696 4151 Size: 32 1481 32 4537 Fragments arranged by size: 4537 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3127 3127 Size: 6082 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6082 PsiI TTA'TAA Cuts at: 3652 3652 Size: 6082 PstI C_TGCA'G Cuts at: 830 3434 830 Size: 2604 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6082 SalI G'TCGA_C Cuts at: 1936 3033 3424 1936 Size: 1097 391 4594 Fragments arranged by size: 4594 1097 391 SbfI CC_TGCA'GG Cuts at: 3434 3434 Size: 6082 ScaI AGT'ACT Cuts at: 2222 4330 6042 2222 Size: 2108 1712 2262 Fragments arranged by size: 2262 2108 1712 SexAI A'CCwGG_T Cuts at: 2575 2575 Size: 6082 SfcI C'TryA_G Cuts at: 826 1045 3000 3430 4565 5246 5437 826 Size: 219 1955 430 1135 681 191 1471 Fragments arranged by size: 1955 1471 1135 681 430 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6082 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6082 SmaI CCC'GGG Cuts at: 1872 3409 1872 Size: 1537 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4186 5087 5349 820 Size: 197 926 2243 901 262 1553 Fragments arranged by size: 2243 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3113 3873 493 Size: 2620 760 2702 Fragments arranged by size: 2702 2620 760 SpeI A'CTAG_T Cuts at: 152 152 Size: 6082 SphI G_CATG'C Cuts at: 1132 2522 3440 1132 Size: 1390 918 3774 Fragments arranged by size: 3774 1390 918 SspI AAT'ATT Cuts at: 5 52 4006 5 Size: 47 3954 2081 Fragments arranged by size: 3954 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6082 StyI C'CwwG_G Cuts at: 513 1065 2138 2270 2740 3521 513 Size: 552 1073 132 470 781 3074 Fragments arranged by size: 3074 1073 781 552 470 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2106 4109 4126 4279 4464 4909 325 Size: 1255 526 2003 17 153 185 445 1498 Fragments arranged by size: 2003 1498 1255 526 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2220 2718 4328 Size: 274 80 33 51 1443 243 498 1610 Cuts at: 4328 6040 96 Size: 1712 138 Fragments arranged by size: 1712 1610 1443 498 274 243 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2342 2374 3165 3197 6042 6074 2342 Size: 32 791 32 2845 32 2350 Fragments arranged by size: 2845 2350 791 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6082 XbaI T'CTAG_A Cuts at: 3418 3418 Size: 6082 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6082 XmaI C'CCGG_G Cuts at: 1870 3407 1870 Size: 1537 4545 XmnI GAAnn'nnTTC Cuts at: 2066 4211 2066 Size: 2145 3937 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3890 276 Size: 53 83 186 936 2356 2468 Fragments arranged by size: 2468 2356 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI