Restriction map for
pFN21AE4439
(Circular) (Six-base) MAPSORT of: pFN21AE4439.seq Check: 8185 from: 1 to: 6073
pFN21AE4439 6073 bp
With 182 enzymes: SgfI *
March 28, 2012 14:01 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3883 278
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3416 1937
Size: 1479 4594
Acc65I G'GTAC_C
Cuts at: 3394 3394
Size: 6073
AclI AA'CG_TT
Cuts at: 1563 2980 4200 4573 1563
Size: 1417 1220 373 3063
Fragments arranged by size:
3063 1417 1220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4136 5520 5788 1632
Size: 2504 1384 268 1917
Fragments arranged by size:
2504 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5876
AflIII A'CryG_T
Cuts at: 1203 2136 4972 5949 1203
Size: 933 2836 977 1327
Fragments arranged by size:
2836 1327 977 933
AgeI A'CCGG_T
Cuts at: 4904 4904
Size: 6073
AhdI GACnn_n'nnGTC
Cuts at: 2013 4802 2013
Size: 2789 3284
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6073
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2424 1184
Size: 1240 4833
AlwNI CAG_nnn'CTG
Cuts at: 2973 3081 3243 3816 5388 2973
Size: 108 162 573 1572 3658
Fragments arranged by size:
3658 1572 573 162 108
ApaI G_GGCC'C
Cuts at: 3261 3261
Size: 6073
ApaLI G'TGCA_C
Cuts at: 4130 5286 4130
Size: 1156 4917
ApoI r'AATT_y
Cuts at: 1493 2092 2693 3382 3608 3760 5869 1493
Size: 599 601 689 226 152 2109 1697
Fragments arranged by size:
2109 1697 689 601 599 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2454 4627 160
Size: 872 1422 2173 1606
Fragments arranged by size:
2173 1606 1422 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
AvaI C'yCGr_G
Cuts at: 1870 1943 2732 3398 1870
Size: 73 789 666 4545
Fragments arranged by size:
4545 789 666 73
BamHI G'GATC_C
Cuts at: 2319 2337 3403 3770 2319
Size: 18 1066 367 4622
Fragments arranged by size:
4622 1066 367 18
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2465 3394 4849 618
Size: 325 217 627 678 929 1455 1842
Fragments arranged by size:
1842 1455 929 678 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3261 3392 1391
Size: 1870 131 4072
Fragments arranged by size:
4072 1870 131
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3163 928
Size: 971 1264 3838
Fragments arranged by size:
3838 1264 971
BbvCI CC'TCA_GC
Cuts at: 3279 3279
Size: 6073
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3164 3198 3235 3269 4264 4298 1903
Size: 34 1227 34 37 34 995 34 3678
Fragments arranged by size:
3678 1227 995 37 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3965 5175 3965
Size: 1210 4863
BclI T'GATC_A
Cuts at: 1548 2376 2436 1548
Size: 828 60 5185
Fragments arranged by size:
5185 828 60
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2553 2570 3305
Size: 107 122 71 631 605 881 17 735
Cuts at: 3305 4684 136
Size: 1379 1525
Fragments arranged by size:
1525 1379 881 735 631 605 122 107
71 17
BglII A'GATC_T
Cuts at: 6068 6068
Size: 6073
BlpI GC'TnA_GC
Cuts at: 3490 3490
Size: 6073
Bme1580I G_kGCm'C
Cuts at: 1790 3261 4134 5290 1790
Size: 1471 873 1156 2573
Fragments arranged by size:
2573 1471 1156 873
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6073
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2320 3344 3804
Size: 416 53 83 399 24 903 1024 460
Cuts at: 3804 4762 442
Size: 958 1753
Fragments arranged by size:
1753 1024 958 903 460 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6073
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 6073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2823 4733 1239
Size: 1584 1910 2579
Fragments arranged by size:
2579 1910 1584
Bpu10I CC'TnA_GC
Cuts at: 2217 3279 2217
Size: 1062 5011
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2702 4198 5063 5361 2702
Size: 1496 865 298 3414
Fragments arranged by size:
3414 1496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2738 3281 4736 882
Size: 617 1239 543 1455 2219
Fragments arranged by size:
2219 1455 1239 617 543
BsaAI yAC'GTr
Cuts at: 493 1204 3864 493
Size: 711 2660 2702
Fragments arranged by size:
2702 2660 711
BsaBI GATnn'nnATC
Cuts at: 2441 6067 2441
Size: 3626 2447
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2181 2189 3880 4262
Size: 53 83 186 936 648 8 1691 382
Cuts at: 4262 4897 275
Size: 635 1451
Fragments arranged by size:
1691 1451 936 648 635 382 186 83
53 8
BsaWI w'CCGG_w
Cuts at: 4506 4904 5178 5325 5736 4506
Size: 398 274 147 411 4843
Fragments arranged by size:
4843 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2285 2315 2735 2765 2796
Size: 30 165 30 882 30 420 30 31
Cuts at: 2796 2826 1178
Size: 30 4425
Fragments arranged by size:
4425 882 420 165 31 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2789 1186
Size: 1603 4470
BseYI C'CCAG_C
Cuts at: 2065 5276 5772 2065
Size: 3211 496 2366
Fragments arranged by size:
3211 2366 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2420 1163
Size: 573 684 4816
Fragments arranged by size:
4816 684 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3523 4284 5312 1540
Size: 275 186 1522 761 1028 2301
Fragments arranged by size:
2301 1522 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2451 3180 4134 4219 5290 2451
Size: 729 954 85 1071 3234
Fragments arranged by size:
3234 1071 954 729 85
BsiWI C'GTAC_G
Cuts at: 2332 2332
Size: 6073
BsmI GAATG_Cn'
Cuts at: 3584 3677 3584
Size: 93 5980
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2451 3180 3261 3392 4134 4219 5290
Size: 399 661 729 81 131 742 85 1071
Cuts at: 5290 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 729 661 399 131 85
81
BspEI T'CCGG_A
Cuts at: 5736 5736
Size: 6073
BspHI T'CATG_A
Cuts at: 2100 2253 2532 3962 5692 2100
Size: 153 279 1430 1730 2481
Fragments arranged by size:
2481 1730 1430 279 153
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3428 844
Size: 2584 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2221 3960 1394
Size: 429 398 1739 3507
Fragments arranged by size:
3507 1739 429 398
BsrDI GCAATG_nn'
Cuts at: 65 1248 4568 4742 65
Size: 1183 3320 174 1396
Fragments arranged by size:
3320 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4717 4904 1700
Size: 110 2907 187 2869
Fragments arranged by size:
2907 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6073
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6073
BssSI C'ACGA_G
Cuts at: 4133 5145 4133
Size: 1012 5061
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6073
BstBI TT'CG_AA
Cuts at: 4877 4877
Size: 6073
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6073
BstXI CCAn_nnnn'nTGG
Cuts at: 2365 2365
Size: 6073
BstYI r'GATC_y
Cuts at: 1972 2319 2337 3403 3770 4155 4172 5624 5710
Size: 347 18 1066 367 385 17 1452 86
Cuts at: 5710 5722 6068 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1066 385 367 347 346 86
18 17 12
Bsu36I CC'TnA_GG
Cuts at: 2025 2025
Size: 6073
BtgI C'CryG_G
Cuts at: 513 1065 2087 2568 513
Size: 552 1022 481 4018
Fragments arranged by size:
4018 1022 552 481
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2941 487
Size: 683 56 1715 3619
Fragments arranged by size:
3619 1715 683 56
BtsI GCAGTG_nn'
Cuts at: 2160 2379 3122 3589 4383 4403 2160
Size: 219 743 467 794 20 3830
Fragments arranged by size:
3830 794 743 467 219 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6073
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6038
DraI TTT'AAA
Cuts at: 3377 3377
Size: 6073
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5080 809
Size: 507 3764 1802
Fragments arranged by size:
3764 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2106 2355 2539
Size: 54 1425 50 275 18 276 249 184
Cuts at: 2539 2571 3520 4409 6038 8
Size: 32 949 889 1629 43
Fragments arranged by size:
1629 1425 949 889 276 275 249 184
54 50 43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 3520 1537
Size: 275 1708 4090
Fragments arranged by size:
4090 1708 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4003 1353
Size: 2650 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4660 5034 5180 387
Size: 1064 138 3071 374 146 1280
Fragments arranged by size:
3071 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2823 4136 4733 5520 5788 1239
Size: 393 1191 1313 597 787 268 1524
Fragments arranged by size:
1524 1313 1191 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2393 2393
Size: 6073
EcoO109I rG'GnC_Cy
Cuts at: 2545 2729 2545
Size: 184 5889
EcoRI G'AATT_C
Cuts at: 2693 3382 3760 2693
Size: 689 378 5006
Fragments arranged by size:
5006 689 378
FspI TGC'GCA
Cuts at: 4579 4579
Size: 6073
HaeII r_GCGC'y
Cuts at: 1678 5220 1678
Size: 3542 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2095 2127 2796
Size: 32 135 32 427 32 64 32 669
Cuts at: 2796 2828 2933 2965 4714 4746 4788 4820 1373
Size: 32 105 32 1749 32 42 32 2626
Fragments arranged by size:
2626 1749 669 427 135 105 64 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3417 3663 669
Size: 988 281 1479 246 3079
Fragments arranged by size:
3079 1479 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2720 748
Size: 1972 4101
HpaI GTT'AAC
Cuts at: 3663 3663
Size: 6073
KpnI G_GTAC'C
Cuts at: 3398 3398
Size: 6073
MfeI C'AATT_G
Cuts at: 3366 3672 3366
Size: 306 5767
MluI A'CGCG_T
Cuts at: 5949 5949
Size: 6073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2733 2841 3246 5187 5371 1305
Size: 1428 108 405 1941 184 2007
Fragments arranged by size:
2007 1941 1428 405 184 108
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2357 2541 2573 10
Size: 54 1425 343 525 184 32 3510
Fragments arranged by size:
3510 1425 525 343 184 54 32
MslI CAynn'nnrTG
Cuts at: 518 1166 2390 2432 2918 2993 4031 4390 4549
Size: 648 1224 42 486 75 1038 359 159
Cuts at: 4549 518
Size: 2042
Fragments arranged by size:
2042 1224 1038 648 486 359 159 75
42
MspA1I CmG'CkG
Cuts at: 1606 2404 3050 3081 3278 3489 4166 5314 5559
Size: 798 646 31 197 211 677 1148 245
Cuts at: 5559 1606
Size: 2120
Fragments arranged by size:
2120 1148 798 677 646 245 211 197
31
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5521
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6073
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6073
NotI GC'GGCC_GC
Cuts at: 3520 3520
Size: 6073
NspI r_CATG'y
Cuts at: 1132 1229 2140 3250 3431 4976 1132
Size: 97 911 1110 181 1545 2229
Fragments arranged by size:
2229 1545 1110 911 181 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6073
PciI A'CATG_T
Cuts at: 2136 4972 2136
Size: 2836 3237
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6073
PmeI GTTT'AAAC
Cuts at: 3377 3377
Size: 6073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4142 4174 5655 5687 4142
Size: 32 1481 32 4528
Fragments arranged by size:
4528 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2729 2729
Size: 6073
PshAI GACnn'nnGTC
Cuts at: 1613 3167 1613
Size: 1554 4519
PsiI TTA'TAA
Cuts at: 3643 3643
Size: 6073
PspOMI G'GGCC_C
Cuts at: 3257 3257
Size: 6073
PstI C_TGCA'G
Cuts at: 830 2206 2478 3425 830
Size: 1376 272 947 3478
Fragments arranged by size:
3478 1376 947 272
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6073
PvuII CAG'CTG
Cuts at: 2404 3050 3278 2404
Size: 646 228 5199
Fragments arranged by size:
5199 646 228
SalI G'TCGA_C
Cuts at: 1936 3415 1936
Size: 1479 4594
SbfI CC_TGCA'GG
Cuts at: 2478 3425 2478
Size: 947 5126
ScaI AGT'ACT
Cuts at: 4321 6033 4321
Size: 1712 4361
SfcI C'TryA_G
Cuts at: 826 1045 2202 2474 3421 4556 5237 5428 826
Size: 219 1157 272 947 1135 681 191 1471
Fragments arranged by size:
1471 1157 1135 947 681 272 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6073
SmaI CCC'GGG
Cuts at: 1872 2734 3400 1872
Size: 862 666 4545
Fragments arranged by size:
4545 862 666
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2717 4177 5078 5340 820
Size: 197 926 774 1460 901 262 1553
Fragments arranged by size:
1553 1460 926 901 774 262 197
SnaBI TAC'GTA
Cuts at: 493 3864 493
Size: 3371 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6073
SphI G_CATG'C
Cuts at: 1132 3431 1132
Size: 2299 3774
SspI AAT'ATT
Cuts at: 5 52 3997 5
Size: 47 3945 2081
Fragments arranged by size:
3945 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6073
StyI C'CwwG_G
Cuts at: 513 1065 2154 3512 513
Size: 552 1089 1358 3074
Fragments arranged by size:
3074 1358 1089 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4100 4117 4270 4455 4900 325
Size: 1255 2520 17 153 185 445 1498
Fragments arranged by size:
2520 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4319 6031 96
Size: 274 80 33 51 1443 2342 1712 138
Fragments arranged by size:
2342 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6033 6065 6033
Size: 32 6041
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6073
XbaI T'CTAG_A
Cuts at: 3409 3409
Size: 6073
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6073
XmaI C'CCGG_G
Cuts at: 1870 2732 3398 1870
Size: 862 666 4545
Fragments arranged by size:
4545 862 666
XmnI GAAnn'nnTTC
Cuts at: 2096 4202 2096
Size: 2106 3967
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3881 276
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI