Restriction map for
pFN21AE4438
(Circular) (Six-base) MAPSORT of: pFN21AE4438.seq Check: 9057 from: 1 to: 6070
pFN21AE4438 6070 bp
With 182 enzymes: SgfI *
March 28, 2012 13:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2311 2311
Size: 6070
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3880 278
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3413 1937
Size: 1476 4594
Acc65I G'GTAC_C
Cuts at: 3391 3391
Size: 6070
AclI AA'CG_TT
Cuts at: 1563 4197 4570 1563
Size: 2634 373 3063
Fragments arranged by size:
3063 2634 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2013 2294 4133 5517 5785 1632
Size: 381 281 1839 1384 268 1917
Fragments arranged by size:
1917 1839 1384 381 281 268
AfeI AGC'GCT
Cuts at: 3119 3119
Size: 6070
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5873
AflIII A'CryG_T
Cuts at: 1203 4969 5946 1203
Size: 3766 977 1327
Fragments arranged by size:
3766 1327 977
AgeI A'CCGG_T
Cuts at: 4901 4901
Size: 6070
AhdI GACnn_n'nnGTC
Cuts at: 2686 4799 2686
Size: 2113 3957
AleI CACnn'nnGTG
Cuts at: 1166 2030 1166
Size: 864 5206
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6070
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3032 3064 3032
Size: 32 6038
AlwNI CAG_nnn'CTG
Cuts at: 3813 5385 3813
Size: 1572 4498
ApaLI G'TGCA_C
Cuts at: 2444 2914 4127 5283 2444
Size: 470 1213 1156 3231
Fragments arranged by size:
3231 1213 1156 470
ApoI r'AATT_y
Cuts at: 1493 2303 2660 3011 3379 3605 3757 5866 1493
Size: 810 357 351 368 226 152 2109 1697
Fragments arranged by size:
2109 1697 810 368 357 351 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4624 160
Size: 872 3592 1606
Fragments arranged by size:
3592 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
AvaI C'yCGr_G
Cuts at: 1870 1943 2064 3395 1870
Size: 73 121 1331 4545
Fragments arranged by size:
4545 1331 121 73
BamHI G'GATC_C
Cuts at: 3400 3767 3400
Size: 367 5703
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2719 3098 3391 4846 618
Size: 325 217 627 932 379 293 1455 1842
Fragments arranged by size:
1842 1455 932 627 379 325 293 217
BanII G_rGCy'C
Cuts at: 1391 3389 1391
Size: 1998 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5099
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2416 2450 4261 4295 1903
Size: 34 479 34 1811 34 3678
Fragments arranged by size:
3678 1811 479 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3962 5172 3962
Size: 1210 4860
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6070
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4681 136
Size: 107 122 71 631 605 3009 1525
Fragments arranged by size:
3009 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6065 6065
Size: 6070
BlpI GC'TnA_GC
Cuts at: 3487 3487
Size: 6070
Bme1580I G_kGCm'C
Cuts at: 1790 2448 2724 2918 3189 4131 5287 1790
Size: 658 276 194 271 942 1156 2573
Fragments arranged by size:
2573 1156 942 658 276 271 194
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6070
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2692 3801 4759
Size: 416 53 83 399 24 1275 1109 958
Cuts at: 4759 442
Size: 1753
Fragments arranged by size:
1753 1275 1109 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6070
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 6070
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3044 3380 4730 1239
Size: 1805 336 1350 2579
Fragments arranged by size:
2579 1805 1350 336
Bpu10I CC'TnA_GC
Cuts at: 2519 2519
Size: 6070
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4195 5060 5358 4195
Size: 865 298 4907
Fragments arranged by size:
4907 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4733 882
Size: 617 3234 2219
Fragments arranged by size:
3234 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2392 2731 3861 493
Size: 711 1188 339 1130 2702
Fragments arranged by size:
2702 1188 1130 711 339
BsaBI GATnn'nnATC
Cuts at: 6064 6064
Size: 6070
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3877 4259 4894 275
Size: 53 83 186 936 2344 382 635 1451
Fragments arranged by size:
2344 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4503 4901 5175 5322 5733 4503
Size: 398 274 147 411 4840
Fragments arranged by size:
4840 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2834 2864 1178
Size: 30 165 30 1431 30 4384
Fragments arranged by size:
4384 1431 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2012 2192 2780 3100 3326 1186
Size: 826 180 588 320 226 3930
Fragments arranged by size:
3930 826 588 320 226 180
BseYI C'CCAG_C
Cuts at: 2035 2403 2647 2712 2723 3114 5273 5769 2035
Size: 368 244 65 11 391 2159 496 2336
Fragments arranged by size:
2336 2159 496 391 368 244 65 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2683 2726 1163
Size: 573 947 43 4507
Fragments arranged by size:
4507 947 573 43
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3520 4281 5309 1540
Size: 275 186 1519 761 1028 2301
Fragments arranged by size:
2301 1519 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2448 2532 2918 4131 4216 5287 2448
Size: 84 386 1213 85 1071 3231
Fragments arranged by size:
3231 1213 1071 386 85 84
BsmI GAATG_Cn'
Cuts at: 3581 3674 3581
Size: 93 5977
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2448 2532 2724 2918 3189 3389 4131
Size: 399 658 84 192 194 271 200 742
Cuts at: 4131 4216 5287 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 658 399 271 200 194
192 85 84
BspEI T'CCGG_A
Cuts at: 5733 5733
Size: 6070
BspHI T'CATG_A
Cuts at: 3959 5689 3959
Size: 1730 4340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2311 3072 3425 844
Size: 1467 761 353 3489
Fragments arranged by size:
3489 1467 761 353
BsrBI CCG'CTC
Cuts at: 1394 1823 2895 3169 3364 3957 1394
Size: 429 1072 274 195 593 3507
Fragments arranged by size:
3507 1072 593 429 274 195
BsrDI GCAATG_nn'
Cuts at: 65 1248 4565 4739 65
Size: 1183 3317 174 1396
Fragments arranged by size:
3317 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4714 4901 1700
Size: 110 2904 187 2869
Fragments arranged by size:
2904 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6070
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6070
BssSI C'ACGA_G
Cuts at: 2116 4130 5142 2116
Size: 2014 1012 3044
Fragments arranged by size:
3044 2014 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6070
BstBI TT'CG_AA
Cuts at: 4874 4874
Size: 6070
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6070
BstYI r'GATC_y
Cuts at: 1972 2181 2622 3400 3767 4152 4169 5621 5707
Size: 209 441 778 367 385 17 1452 86
Cuts at: 5707 5719 6065 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 778 441 385 367 346 209
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2512 513
Size: 552 1447 4071
Fragments arranged by size:
4071 1447 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2538 487
Size: 683 56 1312 4019
Fragments arranged by size:
4019 1312 683 56
BtsI GCAGTG_nn'
Cuts at: 3586 4380 4400 3586
Size: 794 20 5256
Fragments arranged by size:
5256 794 20
ClaI AT'CG_AT
Cuts at: 1554 2205 1554
Size: 651 5419
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6035
DraI TTT'AAA
Cuts at: 3374 3374
Size: 6070
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5077 809
Size: 507 3761 1802
Fragments arranged by size:
3761 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2610 3517 4406
Size: 54 1425 50 275 18 780 907 889
Cuts at: 4406 6035 8
Size: 1629 43
Fragments arranged by size:
1629 1425 907 889 780 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3517 1537
Size: 275 1705 4090
Fragments arranged by size:
4090 1705 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4000 1353
Size: 2647 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2138 2311 4657 5031 5177 387
Size: 1064 138 549 173 2346 374 146 1280
Fragments arranged by size:
2346 1280 1064 549 374 173 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2013 2294 3044 3380 4133 4730 5517
Size: 393 381 281 750 336 753 597 787
Cuts at: 5517 5785 1239
Size: 268 1524
Fragments arranged by size:
1524 787 753 750 597 393 381 336
281 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3140 3140
Size: 6070
EcoO109I rG'GnC_Cy
Cuts at: 2688 2688
Size: 6070
EcoRI G'AATT_C
Cuts at: 2660 3011 3379 3757 2660
Size: 351 368 378 4973
Fragments arranged by size:
4973 378 368 351
FspI TGC'GCA
Cuts at: 4576 4576
Size: 6070
HaeII r_GCGC'y
Cuts at: 1678 3121 5217 1678
Size: 1443 2096 2531
Fragments arranged by size:
2531 2096 1443
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2672 2704 4711 4743 4785
Size: 32 135 32 1100 32 2007 32 42
Cuts at: 4785 4817 1373
Size: 32 2626
Fragments arranged by size:
2626 2007 1100 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3414 3660 669
Size: 988 281 1476 246 3079
Fragments arranged by size:
3079 1476 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6070
HpaI GTT'AAC
Cuts at: 3660 3660
Size: 6070
KpnI G_GTAC'C
Cuts at: 3395 3395
Size: 6070
MfeI C'AATT_G
Cuts at: 3669 3669
Size: 6070
MluI A'CGCG_T
Cuts at: 5946 5946
Size: 6070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2289 5184 5368 1305
Size: 984 2895 184 2007
Fragments arranged by size:
2895 2007 984 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2612 10
Size: 54 1425 343 780 3468
Fragments arranged by size:
3468 1425 780 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2030 2390 2705 4028 4387 4546 518
Size: 648 864 360 315 1323 359 159 2042
Fragments arranged by size:
2042 1323 864 648 360 359 315 159
MspA1I CmG'CkG
Cuts at: 1606 2241 2407 2716 3486 4163 5311 5556 1606
Size: 635 166 309 770 677 1148 245 2120
Fragments arranged by size:
2120 1148 770 677 635 309 245 166
NcoI C'CATG_G
Cuts at: 513 1065 2512 513
Size: 552 1447 4071
Fragments arranged by size:
4071 1447 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6070
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6070
NotI GC'GGCC_GC
Cuts at: 3517 3517
Size: 6070
NspI r_CATG'y
Cuts at: 1132 1229 2905 3428 4973 1132
Size: 97 1676 523 1545 2229
Fragments arranged by size:
2229 1676 1545 523 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6070
PciI A'CATG_T
Cuts at: 4969 4969
Size: 6070
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2052 1108
Size: 944 5126
PfoI T'CCnGG_A
Cuts at: 2655 2655
Size: 6070
PmeI GTTT'AAAC
Cuts at: 3374 3374
Size: 6070
PmlI CAC'GTG
Cuts at: 2392 2731 2392
Size: 339 5731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4139 4171 5652 5684 4139
Size: 32 1481 32 4525
Fragments arranged by size:
4525 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2688 2688
Size: 6070
PshAI GACnn'nnGTC
Cuts at: 1613 3074 1613
Size: 1461 4609
PsiI TTA'TAA
Cuts at: 2478 3640 2478
Size: 1162 4908
PstI C_TGCA'G
Cuts at: 830 3422 830
Size: 2592 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6070
PvuII CAG'CTG
Cuts at: 2241 2407 2716 2241
Size: 166 309 5595
Fragments arranged by size:
5595 309 166
SalI G'TCGA_C
Cuts at: 1936 3412 1936
Size: 1476 4594
SanDI GG'GwC_CC
Cuts at: 2688 2688
Size: 6070
SbfI CC_TGCA'GG
Cuts at: 3422 3422
Size: 6070
ScaI AGT'ACT
Cuts at: 4318 6030 4318
Size: 1712 4358
SfcI C'TryA_G
Cuts at: 826 1045 2875 3418 4553 5234 5425 826
Size: 219 1830 543 1135 681 191 1471
Fragments arranged by size:
1830 1471 1135 681 543 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6070
SmaI CCC'GGG
Cuts at: 1872 2066 3397 1872
Size: 194 1331 4545
Fragments arranged by size:
4545 1331 194
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4174 5075 5337 820
Size: 197 926 2231 901 262 1553
Fragments arranged by size:
2231 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3861 493
Size: 3368 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6070
SphI G_CATG'C
Cuts at: 1132 3428 1132
Size: 2296 3774
SspI AAT'ATT
Cuts at: 5 52 3994 5
Size: 47 3942 2081
Fragments arranged by size:
3942 2081 47
StuI AGG'CCT
Cuts at: 1517 2739 3036 1517
Size: 1222 297 4551
Fragments arranged by size:
4551 1222 297
StyI C'CwwG_G
Cuts at: 513 1065 2512 3509 513
Size: 552 1447 997 3074
Fragments arranged by size:
3074 1447 997 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2088 3229 4097 4114 4267 4452 4897
Size: 1255 508 1141 868 17 153 185 445
Cuts at: 4897 325
Size: 1498
Fragments arranged by size:
1498 1255 1141 868 508 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4316 6028 96
Size: 274 80 33 51 1443 2339 1712 138
Fragments arranged by size:
2339 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6030 6062 6030
Size: 32 6038
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6070
XbaI T'CTAG_A
Cuts at: 2980 3406 2980
Size: 426 5644
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6070
XmaI C'CCGG_G
Cuts at: 1870 2064 3395 1870
Size: 194 1331 4545
Fragments arranged by size:
4545 1331 194
XmnI GAAnn'nnTTC
Cuts at: 2597 2870 4199 2597
Size: 273 1329 4468
Fragments arranged by size:
4468 1329 273
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3878 276
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI