Restriction map for
pFN21AE4437
(Circular) (Six-base) MAPSORT of: pFN21AE4437.seq Check: 940 from: 1 to: 6064
pFN21AE4437 6064 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3874 278
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3407 1937
Size: 1470 4594
Acc65I G'GTAC_C
Cuts at: 3385 3385
Size: 6064
AclI AA'CG_TT
Cuts at: 1563 4191 4564 1563
Size: 2628 373 3063
Fragments arranged by size:
3063 2628 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2032 3341 4127 5511 5779 1632
Size: 400 1309 786 1384 268 1917
Fragments arranged by size:
1917 1384 1309 786 400 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5867
AflIII A'CryG_T
Cuts at: 1203 3099 4963 5940 1203
Size: 1896 1864 977 1327
Fragments arranged by size:
1896 1864 1327 977
AgeI A'CCGG_T
Cuts at: 4895 4895
Size: 6064
AhdI GACnn_n'nnGTC
Cuts at: 2983 4793 2983
Size: 1810 4254
AleI CACnn'nnGTG
Cuts at: 1166 2576 2972 1166
Size: 1410 396 4258
Fragments arranged by size:
4258 1410 396
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6064
AlwNI CAG_nnn'CTG
Cuts at: 2092 2110 2134 2476 3807 5379 2092
Size: 18 24 342 1331 1572 2777
Fragments arranged by size:
2777 1572 1331 342 24 18
ApaLI G'TGCA_C
Cuts at: 4121 5277 4121
Size: 1156 4908
ApoI r'AATT_y
Cuts at: 1493 2711 3373 3599 3751 5860 1493
Size: 1218 662 226 152 2109 1697
Fragments arranged by size:
2109 1697 1218 662 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4618 160
Size: 872 3586 1606
Fragments arranged by size:
3586 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
AvaI C'yCGr_G
Cuts at: 1870 1943 2053 2841 3337 3389 1870
Size: 73 110 788 496 52 4545
Fragments arranged by size:
4545 788 496 110 73 52
BamHI G'GATC_C
Cuts at: 2082 3236 3394 3761 2082
Size: 1154 158 367 4385
Fragments arranged by size:
4385 1154 367 158
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2339 2384 2924 3385 4840
Size: 325 217 627 552 45 540 461 1455
Cuts at: 4840 618
Size: 1842
Fragments arranged by size:
1842 1455 627 552 540 461 325 217
45
BanII G_rGCy'C
Cuts at: 1391 2262 2298 3344 3383 1391
Size: 871 36 1046 39 4072
Fragments arranged by size:
4072 1046 871 39 36
BbeI G_GCGC'C
Cuts at: 2388 2388
Size: 6064
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2276 928
Size: 971 377 4716
Fragments arranged by size:
4716 971 377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4255 4289 1903
Size: 34 2318 34 3678
Fragments arranged by size:
3678 2318 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3956 5166 3956
Size: 1210 4854
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6064
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4675 136
Size: 107 122 71 631 605 3003 1525
Fragments arranged by size:
3003 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3006 6059 3006
Size: 3053 3011
BlpI GC'TnA_GC
Cuts at: 3481 3481
Size: 6064
Bme1580I G_kGCm'C
Cuts at: 1790 2869 2927 4125 5281 1790
Size: 1079 58 1198 1156 2573
Fragments arranged by size:
2573 1198 1156 1079 58
BmgBI CAC'GTC
Cuts at: 1318 2683 2974 1318
Size: 1365 291 4408
Fragments arranged by size:
4408 1365 291
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2327 2464 2489
Size: 416 53 83 399 24 910 137 25
Cuts at: 2489 2977 3795 4753 442
Size: 488 818 958 1753
Fragments arranged by size:
1753 958 910 818 488 416 399 137
83 53 25 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6064
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3286 3286
Size: 6064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2651 3068 3091 4724 1239
Size: 875 537 417 23 1633 2579
Fragments arranged by size:
2579 1633 875 537 417 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2399 2519 2780 4189 5054 5352 2399
Size: 120 261 1409 865 298 3111
Fragments arranged by size:
3111 1409 865 298 261 120
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2513 4727 882
Size: 617 1014 2214 2219
Fragments arranged by size:
2219 2214 1014 617
BsaAI yAC'GTr
Cuts at: 493 1204 2659 3855 493
Size: 711 1455 1196 2702
Fragments arranged by size:
2702 1455 1196 711
BsaBI GATnn'nnATC
Cuts at: 6058 6058
Size: 6064
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2273 2385 3871 4253
Size: 53 83 186 936 740 112 1486 382
Cuts at: 4253 4888 275
Size: 635 1451
Fragments arranged by size:
1486 1451 936 740 635 382 186 112
83 53
BsaWI w'CCGG_w
Cuts at: 2079 4497 4895 5169 5316 5727 2079
Size: 2418 398 274 147 411 2416
Fragments arranged by size:
2418 2416 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2183 2204 2213 2234 3089
Size: 30 165 30 780 21 9 21 855
Cuts at: 3089 3119 1178
Size: 30 4123
Fragments arranged by size:
4123 855 780 165 30 30 30 21
21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2204 2225 3017 3065 1186
Size: 1018 21 792 48 4185
Fragments arranged by size:
4185 1018 792 48 21
BseYI C'CCAG_C
Cuts at: 2635 3357 5267 5763 2635
Size: 722 1910 496 2936
Fragments arranged by size:
2936 1910 722 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2144 1163
Size: 573 408 5083
Fragments arranged by size:
5083 573 408
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3514 4275 5303 1540
Size: 275 186 1513 761 1028 2301
Fragments arranged by size:
2301 1513 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2298 4125 4210 5281 2298
Size: 1827 85 1071 3081
Fragments arranged by size:
3081 1827 1071 85
BsmI GAATG_Cn'
Cuts at: 3575 3668 3575
Size: 93 5971
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2262 2298 2869 2927 3344 3383 4125
Size: 399 472 36 571 58 417 39 742
Cuts at: 4125 4210 5281 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 571 472 417 399 85
58 39 36
BspEI T'CCGG_A
Cuts at: 2079 5727 2079
Size: 3648 2416
BspHI T'CATG_A
Cuts at: 2592 2715 3953 5683 2592
Size: 123 1238 1730 2973
Fragments arranged by size:
2973 1730 1238 123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2166 2741 3419 844
Size: 1322 575 678 3489
Fragments arranged by size:
3489 1322 678 575
BsrBI CCG'CTC
Cuts at: 1394 1823 3282 3951 1394
Size: 429 1459 669 3507
Fragments arranged by size:
3507 1459 669 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4559 4733 65
Size: 1183 3311 174 1396
Fragments arranged by size:
3311 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4708 4895 1700
Size: 110 2898 187 2869
Fragments arranged by size:
2898 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2559 96
Size: 2463 3601
BssHII G'CGCG_C
Cuts at: 1925 2032 2142 1925
Size: 107 110 5847
Fragments arranged by size:
5847 110 107
BssSI C'ACGA_G
Cuts at: 4124 5136 4124
Size: 1012 5052
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3202 794
Size: 2408 3656
BstBI TT'CG_AA
Cuts at: 4868 4868
Size: 6064
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6064
BstYI r'GATC_y
Cuts at: 1972 2082 2485 3006 3236 3394 3761 4146 4163
Size: 110 403 521 230 158 367 385 17
Cuts at: 4163 5615 5701 5713 6059 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 521 403 385 367 346 230
158 110 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2250 2262 2376 2523 3244 513
Size: 552 1185 12 114 147 721 3333
Fragments arranged by size:
3333 1185 721 552 147 114 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2374 2683 487
Size: 683 56 1148 309 3868
Fragments arranged by size:
3868 1148 683 309 56
BtsI GCAGTG_nn'
Cuts at: 2412 3580 4374 4394 2412
Size: 1168 794 20 4082
Fragments arranged by size:
4082 1168 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6064
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6029
DraI TTT'AAA
Cuts at: 3368 3368
Size: 6064
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5071 809
Size: 507 3755 1802
Fragments arranged by size:
3755 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2725 3165
Size: 54 1425 50 275 18 318 577 440
Cuts at: 3165 3345 3511 4400 6029 8
Size: 180 166 889 1629 43
Fragments arranged by size:
1629 1425 889 577 440 318 275 180
166 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3511 1537
Size: 275 1699 4090
Fragments arranged by size:
4090 1699 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2639 3248 3994 1353
Size: 1286 609 746 3423
Fragments arranged by size:
3423 1286 746 609
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4651 5025 5171 387
Size: 1064 138 3062 374 146 1280
Fragments arranged by size:
3062 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2296 2296
Size: 6064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2032 2114 2651 3068 3091 3341 4127
Size: 393 400 82 537 417 23 250 786
Cuts at: 4127 4724 5511 5779 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 786 597 537 417 400 393
268 250 82 23
EcoNI CCTnn'n_nnAGG
Cuts at: 2114 2480 2897 3050 2114
Size: 366 417 153 5128
Fragments arranged by size:
5128 417 366 153
EcoO109I rG'GnC_Cy
Cuts at: 2061 2061
Size: 6064
EcoRI G'AATT_C
Cuts at: 3373 3751 3373
Size: 378 5686
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3274 3274
Size: 6064
FspI TGC'GCA
Cuts at: 3041 4570 3041
Size: 1529 4535
HaeII r_GCGC'y
Cuts at: 1678 2102 2110 2388 5211 1678
Size: 424 8 278 2823 2531
Fragments arranged by size:
2823 2531 424 278 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2969 3001 3256 3288 4705
Size: 32 135 32 1397 32 255 32 1417
Cuts at: 4705 4737 4779 4811 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1417 1397 255 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3408 3654 669
Size: 988 281 1470 246 3079
Fragments arranged by size:
3079 1470 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6064
HpaI GTT'AAC
Cuts at: 3654 3654
Size: 6064
KasI G'GCGC_C
Cuts at: 2384 2384
Size: 6064
KpnI G_GTAC'C
Cuts at: 3389 3389
Size: 6064
MfeI C'AATT_G
Cuts at: 3663 3663
Size: 6064
MluI A'CGCG_T
Cuts at: 5940 5940
Size: 6064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5178 5362 1305
Size: 3873 184 2007
Fragments arranged by size:
3873 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2727 10
Size: 54 1425 343 895 3347
Fragments arranged by size:
3347 1425 895 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2166 2576 2972 4022 4381 4540 518
Size: 648 1000 410 396 1050 359 159 2042
Fragments arranged by size:
2042 1050 1000 648 410 396 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2092 2252 3228 3480 4157 5305 5550
Size: 465 21 160 976 252 677 1148 245
Cuts at: 5550 1606
Size: 2120
Fragments arranged by size:
2120 1148 976 677 465 252 245 160
21
NarI GG'CG_CC
Cuts at: 2385 2385
Size: 6064
NcoI C'CATG_G
Cuts at: 513 1065 2376 3244 513
Size: 552 1311 868 3333
Fragments arranged by size:
3333 1311 868 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6064
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6064
NotI GC'GGCC_GC
Cuts at: 3511 3511
Size: 6064
NspI r_CATG'y
Cuts at: 1132 1229 3103 3422 4967 1132
Size: 97 1874 319 1545 2229
Fragments arranged by size:
2229 1874 1545 319 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6064
PciI A'CATG_T
Cuts at: 3099 4963 3099
Size: 1864 4200
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2092 2377 2476 2785 2911 1108
Size: 984 285 99 309 126 4261
Fragments arranged by size:
4261 984 309 285 126 99
PfoI T'CCnGG_A
Cuts at: 2115 2115
Size: 6064
PmeI GTTT'AAAC
Cuts at: 3368 3368
Size: 6064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4165 5646 5678 4133
Size: 32 1481 32 4519
Fragments arranged by size:
4519 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2061 2061
Size: 6064
PshAI GACnn'nnGTC
Cuts at: 1613 2621 1613
Size: 1008 5056
PsiI TTA'TAA
Cuts at: 3634 3634
Size: 6064
PstI C_TGCA'G
Cuts at: 830 2076 2106 2398 3416 830
Size: 1246 30 292 1018 3478
Fragments arranged by size:
3478 1246 1018 292 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6064
PvuII CAG'CTG
Cuts at: 2092 2092
Size: 6064
SacI G_AGCT'C
Cuts at: 2298 2298
Size: 6064
SacII CC_GC'GG
Cuts at: 2253 2253
Size: 6064
SalI G'TCGA_C
Cuts at: 1936 3406 1936
Size: 1470 4594
SapI GCTCTTCn'nnn_
Cuts at: 3248 3248
Size: 6064
SbfI CC_TGCA'GG
Cuts at: 3416 3416
Size: 6064
ScaI AGT'ACT
Cuts at: 4312 6024 4312
Size: 1712 4352
SfcI C'TryA_G
Cuts at: 826 1045 2072 2102 2394 3412 4547 5228 5419
Size: 219 1027 30 292 1018 1135 681 191
Cuts at: 5419 826
Size: 1471
Fragments arranged by size:
1471 1135 1027 1018 681 292 219 191
30
SfoI GGC'GCC
Cuts at: 2386 2386
Size: 6064
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6064
SmaI CCC'GGG
Cuts at: 1872 2843 3391 1872
Size: 971 548 4545
Fragments arranged by size:
4545 971 548
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2414 2534 2795 4168 5069 5331
Size: 197 926 471 120 261 1373 901 262
Cuts at: 5331 820
Size: 1553
Fragments arranged by size:
1553 1373 926 901 471 262 261 197
120
SnaBI TAC'GTA
Cuts at: 493 3855 493
Size: 3362 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6064
SphI G_CATG'C
Cuts at: 1132 3422 1132
Size: 2290 3774
SspI AAT'ATT
Cuts at: 5 52 3988 5
Size: 47 3936 2081
Fragments arranged by size:
3936 2081 47
StuI AGG'CCT
Cuts at: 1517 3298 3318 1517
Size: 1781 20 4263
Fragments arranged by size:
4263 1781 20
StyI C'CwwG_G
Cuts at: 513 1065 2376 3244 3503 513
Size: 552 1311 868 259 3074
Fragments arranged by size:
3074 1311 868 552 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4091 4108 4261 4446 4891 325
Size: 1255 2511 17 153 185 445 1498
Fragments arranged by size:
2511 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2220 2559 4310
Size: 274 80 33 51 1443 243 339 1751
Cuts at: 4310 6022 96
Size: 1712 138
Fragments arranged by size:
1751 1712 1443 339 274 243 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6024 6056 6024
Size: 32 6032
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6064
XbaI T'CTAG_A
Cuts at: 3400 3400
Size: 6064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2374 3157 2374
Size: 783 5281
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6064
XmaI C'CCGG_G
Cuts at: 1870 2841 3389 1870
Size: 971 548 4545
Fragments arranged by size:
4545 971 548
XmnI GAAnn'nnTTC
Cuts at: 2813 3257 4193 2813
Size: 444 936 4684
Fragments arranged by size:
4684 936 444
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3872 276
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI
SrfI SwaI