Restriction map for
pFN21AE4435
(Circular) (Six-base) MAPSORT of: pFN21AE4435.seq Check: 947 from: 1 to: 6061
pFN21AE4435 6061 bp
With 182 enzymes: SgfI *
March 28, 2012 13:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2999 3871 278
Size: 53 83 186 936 1463 872 2468
Fragments arranged by size:
2468 1463 936 872 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3404 1937
Size: 1467 4594
Acc65I G'GTAC_C
Cuts at: 3382 3382
Size: 6061
AclI AA'CG_TT
Cuts at: 1563 4188 4561 1563
Size: 2625 373 3063
Fragments arranged by size:
3063 2625 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2408 4124 5508 5776 1632
Size: 776 1716 1384 268 1917
Fragments arranged by size:
1917 1716 1384 776 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5864
AflIII A'CryG_T
Cuts at: 1203 3131 4960 5937 1203
Size: 1928 1829 977 1327
Fragments arranged by size:
1928 1829 1327 977
AgeI A'CCGG_T
Cuts at: 4892 4892
Size: 6061
AhdI GACnn_n'nnGTC
Cuts at: 4790 4790
Size: 6061
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6061
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6061
AlwNI CAG_nnn'CTG
Cuts at: 3196 3804 5376 3196
Size: 608 1572 3881
Fragments arranged by size:
3881 1572 608
ApaLI G'TGCA_C
Cuts at: 4118 5274 4118
Size: 1156 4905
ApoI r'AATT_y
Cuts at: 1493 3370 3596 3748 5857 1493
Size: 1877 226 152 2109 1697
Fragments arranged by size:
2109 1877 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4615 160
Size: 872 3583 1606
Fragments arranged by size:
3583 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 2255 3386 1870
Size: 73 312 1131 4545
Fragments arranged by size:
4545 1131 312 73
BamHI G'GATC_C
Cuts at: 3391 3758 3391
Size: 367 5694
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2068 2112 3382 4837 618
Size: 325 217 627 281 44 1270 1455 1842
Fragments arranged by size:
1842 1455 1270 627 325 281 217 44
BanII G_rGCy'C
Cuts at: 1391 2262 2457 3349 3380 1391
Size: 871 195 892 31 4072
Fragments arranged by size:
4072 892 871 195 31
BbeI G_GCGC'C
Cuts at: 2072 2116 2072
Size: 44 6017
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2437 2581 2895 3029 928
Size: 971 538 144 314 134 3960
Fragments arranged by size:
3960 971 538 314 144 134
BbvCI CC'TCA_GC
Cuts at: 3036 3269 3036
Size: 233 5828
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4252 4286 1903
Size: 34 2315 34 3678
Fragments arranged by size:
3678 2315 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3953 5163 3953
Size: 1210 4851
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6061
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4672 136
Size: 107 122 71 631 605 3000 1525
Fragments arranged by size:
3000 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2808 2850 6056 2808
Size: 42 3206 2813
Fragments arranged by size:
3206 2813 42
BlpI GC'TnA_GC
Cuts at: 3478 3478
Size: 6061
Bme1580I G_kGCm'C
Cuts at: 1790 4122 5278 1790
Size: 2332 1156 2573
Fragments arranged by size:
2573 2332 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3792 4750 442
Size: 416 53 83 399 24 2375 958 1753
Fragments arranged by size:
2375 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2704 2704
Size: 6061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3161 3218 4721 1239
Size: 1922 57 1503 2579
Fragments arranged by size:
2579 1922 1503 57
Bpu10I CC'TnA_GC
Cuts at: 2889 3036 3269 2889
Size: 147 233 5681
Fragments arranged by size:
5681 233 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2882 4186 5051 5349 2882
Size: 1304 865 298 3594
Fragments arranged by size:
3594 1304 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4724 882
Size: 617 3225 2219
Fragments arranged by size:
3225 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2227 3134 3852 493
Size: 711 1023 907 718 2702
Fragments arranged by size:
2702 1023 907 718 711
BsaBI GATnn'nnATC
Cuts at: 6055 6055
Size: 6061
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2069 2113 2996 3868
Size: 53 83 186 936 536 44 883 872
Cuts at: 3868 4250 4885 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 883 872 635 536 382 186
83 53 44
BsaWI w'CCGG_w
Cuts at: 2421 4494 4892 5166 5313 5724 2421
Size: 2073 398 274 147 411 2758
Fragments arranged by size:
2758 2073 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3191 3221 1178
Size: 30 165 30 1788 30 4018
Fragments arranged by size:
4018 1788 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6061
BseYI C'CCAG_C
Cuts at: 5264 5760 5264
Size: 496 5565
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2289 2969 1163
Size: 573 553 680 4255
Fragments arranged by size:
4255 680 573 553
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2060 3511 4272 5300 1540
Size: 275 186 59 1451 761 1028 2301
Fragments arranged by size:
2301 1451 1028 761 275 186 59
BsiHKAI G_wGCw'C
Cuts at: 2753 2865 3078 3147 3294 4122 4207 5278 2753
Size: 112 213 69 147 828 85 1071 3536
Fragments arranged by size:
3536 1071 828 213 147 112 85 69
BsmI GAATG_Cn'
Cuts at: 3258 3572 3665 3258
Size: 314 93 5654
Fragments arranged by size:
5654 314 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3209 3209
Size: 6061
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2262 2457 2753 2865 3078 3147 3294
Size: 399 472 195 296 112 213 69 147
Cuts at: 3294 3349 3380 4122 4207 5278 1391
Size: 55 31 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 472 399 296 213 195
147 112 85 69 55 31
BspEI T'CCGG_A
Cuts at: 2421 5724 2421
Size: 3303 2758
BspHI T'CATG_A
Cuts at: 3950 5680 3950
Size: 1730 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2025 3349 3416 844
Size: 1181 1324 67 3489
Fragments arranged by size:
3489 1324 1181 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3044 3948 1394
Size: 429 1221 904 3507
Fragments arranged by size:
3507 1221 904 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3280 4556 4730 65
Size: 1183 2032 1276 174 1396
Fragments arranged by size:
2032 1396 1276 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 2059 2071 2570 2865 3087 4705
Size: 110 199 50 12 499 295 222 1618
Cuts at: 4705 4892 1700
Size: 187 2869
Fragments arranged by size:
2869 1618 499 295 222 199 187 110
50 12
BsrGI T'GTAC_A
Cuts at: 96 2631 2667 2742 96
Size: 2535 36 75 3415
Fragments arranged by size:
3415 2535 75 36
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6061
BssSI C'ACGA_G
Cuts at: 4121 5133 4121
Size: 1012 5049
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6061
BstBI TT'CG_AA
Cuts at: 4865 4865
Size: 6061
BstEII G'GTnAC_C
Cuts at: 1184 2290 1184
Size: 1106 4955
BstXI CCAn_nnnn'nTGG
Cuts at: 2975 3247 2975
Size: 272 5789
BstYI r'GATC_y
Cuts at: 1972 2808 2850 3391 3758 4143 4160 5612 5698
Size: 836 42 541 367 385 17 1452 86
Cuts at: 5698 5710 6056 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 836 541 385 367 346 86
42 17 12
Bsu36I CC'TnA_GG
Cuts at: 2047 2047
Size: 6061
BtgI C'CryG_G
Cuts at: 513 1065 2004 2108 2151 2527 3306 513
Size: 552 939 104 43 376 779 3268
Fragments arranged by size:
3268 939 779 552 376 104 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3094 487
Size: 683 56 1868 3454
Fragments arranged by size:
3454 1868 683 56
BtsI GCAGTG_nn'
Cuts at: 2614 3577 4371 4391 2614
Size: 963 794 20 4284
Fragments arranged by size:
4284 963 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2536 2571 529
Size: 35 1972 35 4019
Fragments arranged by size:
4019 1972 35 35
DraI TTT'AAA
Cuts at: 3365 3365
Size: 6061
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5068 809
Size: 507 3752 1802
Fragments arranged by size:
3752 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2057 2310
Size: 54 1425 50 275 18 177 50 253
Cuts at: 2310 2735 3508 4397 6026 8
Size: 425 773 889 1629 43
Fragments arranged by size:
1629 1425 889 773 425 275 253 177
54 50 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2057 3508 1537
Size: 275 245 1451 4090
Fragments arranged by size:
4090 1451 275 245
EarI CTCTTCn'nnn_
Cuts at: 1353 2370 3991 1353
Size: 1017 1621 3423
Fragments arranged by size:
3423 1621 1017
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4648 5022 5168 387
Size: 1064 138 3059 374 146 1280
Fragments arranged by size:
3059 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2408 3161 3218 4124 4721 5508 5776
Size: 393 776 753 57 906 597 787 268
Cuts at: 5776 1239
Size: 1524
Fragments arranged by size:
1524 906 787 776 753 597 393 268
57
EcoNI CCTnn'n_nnAGG
Cuts at: 2966 2966
Size: 6061
EcoO109I rG'GnC_Cy
Cuts at: 2893 3244 2893
Size: 351 5710
EcoRI G'AATT_C
Cuts at: 3370 3748 3370
Size: 378 5683
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2815 2851 2815
Size: 36 6025
FseI GG_CCGG'CC
Cuts at: 2063 3091 2063
Size: 1028 5033
FspI TGC'GCA
Cuts at: 2700 4567 2700
Size: 1867 4194
HaeII r_GCGC'y
Cuts at: 1678 2072 2116 5208 1678
Size: 394 44 3092 2531
Fragments arranged by size:
3092 2531 394 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2936 2968 4702 4734 4776
Size: 32 135 32 1364 32 1734 32 42
Cuts at: 4776 4808 1373
Size: 32 2626
Fragments arranged by size:
2626 1734 1364 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3405 3651 669
Size: 988 281 1467 246 3079
Fragments arranged by size:
3079 1467 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6061
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KasI G'GCGC_C
Cuts at: 2068 2112 2068
Size: 44 6017
KpnI G_GTAC'C
Cuts at: 3386 3386
Size: 6061
MfeI C'AATT_G
Cuts at: 3660 3660
Size: 6061
MluI A'CGCG_T
Cuts at: 5937 5937
Size: 6061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5175 5359 1305
Size: 3870 184 2007
Fragments arranged by size:
3870 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2312 10
Size: 54 1425 343 480 3759
Fragments arranged by size:
3759 1425 480 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2526 2663 4019 4378 4537 518
Size: 648 1360 137 1356 359 159 2042
Fragments arranged by size:
2042 1360 1356 648 359 159 137
MspA1I CmG'CkG
Cuts at: 1606 2110 2706 2740 3477 4154 5302 5547 1606
Size: 504 596 34 737 677 1148 245 2120
Fragments arranged by size:
2120 1148 737 677 596 504 245 34
NaeI GCC'GGC
Cuts at: 2011 2061 2073 2572 3089 2011
Size: 50 12 499 517 4983
Fragments arranged by size:
4983 517 499 50 12
NarI GG'CG_CC
Cuts at: 2069 2113 2069
Size: 44 6017
NcoI C'CATG_G
Cuts at: 513 1065 2004 2527 513
Size: 552 939 523 4047
Fragments arranged by size:
4047 939 552 523
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6061
NgoMIV G'CCGG_C
Cuts at: 2009 2059 2071 2570 3087 2009
Size: 50 12 499 517 4983
Fragments arranged by size:
4983 517 499 50 12
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 3508 3508
Size: 6061
NspI r_CATG'y
Cuts at: 1132 1229 2668 3419 4964 1132
Size: 97 1439 751 1545 2229
Fragments arranged by size:
2229 1545 1439 751 97
PasI CC'CwG_GG
Cuts at: 1348 2968 3349 1348
Size: 1620 381 4060
Fragments arranged by size:
4060 1620 381
PciI A'CATG_T
Cuts at: 4960 4960
Size: 6061
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2603 3196 1108
Size: 1495 593 3973
Fragments arranged by size:
3973 1495 593
PfoI T'CCnGG_A
Cuts at: 2448 2802 2448
Size: 354 5707
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PmlI CAC'GTG
Cuts at: 2227 3134 2227
Size: 907 5154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 5643 5675 4130
Size: 32 1481 32 4516
Fragments arranged by size:
4516 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2893 3244 2893
Size: 351 5710
PshAI GACnn'nnGTC
Cuts at: 1613 2999 1613
Size: 1386 4675
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PstI C_TGCA'G
Cuts at: 830 2042 3413 830
Size: 1212 1371 3478
Fragments arranged by size:
3478 1371 1212
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6061
PvuII CAG'CTG
Cuts at: 2706 2706
Size: 6061
SacII CC_GC'GG
Cuts at: 2111 2111
Size: 6061
SalI G'TCGA_C
Cuts at: 1936 3403 1936
Size: 1467 4594
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 4309 6021 4309
Size: 1712 4349
SexAI A'CCwGG_T
Cuts at: 2286 2286
Size: 6061
SfcI C'TryA_G
Cuts at: 826 1045 2038 3409 4544 5225 5416 826
Size: 219 993 1371 1135 681 191 1471
Fragments arranged by size:
1471 1371 1135 993 681 219 191
SfoI GGC'GCC
Cuts at: 2070 2114 2070
Size: 44 6017
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 2071 1700
Size: 371 5690
SmaI CCC'GGG
Cuts at: 1872 3388 1872
Size: 1516 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2897 4165 5066 5328 820
Size: 197 926 954 1268 901 262 1553
Fragments arranged by size:
1553 1268 954 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3852 493
Size: 3359 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6061
SphI G_CATG'C
Cuts at: 1132 3419 1132
Size: 2287 3774
SspI AAT'ATT
Cuts at: 5 52 3985 5
Size: 47 3933 2081
Fragments arranged by size:
3933 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6061
StyI C'CwwG_G
Cuts at: 513 1065 2004 2527 2983 3240 3500 513
Size: 552 939 523 456 257 260 3074
Fragments arranged by size:
3074 939 552 523 456 260 257
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2330 2846 4088 4105 4258 4443 4888
Size: 1255 750 516 1242 17 153 185 445
Cuts at: 4888 325
Size: 1498
Fragments arranged by size:
1498 1255 1242 750 516 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 2538 2631
Size: 274 80 33 51 1443 345 216 93
Cuts at: 2631 2667 2742 4307 6019 96
Size: 36 75 1565 1712 138
Fragments arranged by size:
1712 1565 1443 345 274 216 138 93
80 75 51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2469 2501 3191 3223 6021 6053 2469
Size: 32 690 32 2798 32 2477
Fragments arranged by size:
2798 2477 690 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2288 1316
Size: 972 5089
XbaI T'CTAG_A
Cuts at: 3397 3397
Size: 6061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2554 2554
Size: 6061
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6061
XmaI C'CCGG_G
Cuts at: 1870 3386 1870
Size: 1516 4545
XmnI GAAnn'nnTTC
Cuts at: 4190 4190
Size: 6061
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2997 3869 276
Size: 53 83 186 936 1463 872 2468
Fragments arranged by size:
2468 1463 936 872 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BstZ17I DraIII EcoICRI EcoRV FspAI NruI NsiI
PacI PspOMI PspXI PsrI RsrII SacI SanDI SapI
SfiI SrfI SwaI