Restriction map for
pFN21AE4432
(Circular) (Six-base) MAPSORT of: pFN21AE4432.seq Check: 4837 from: 1 to: 6058
pFN21AE4432 6058 bp
With 182 enzymes: SgfI *
March 28, 2012 14:01 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2154 3868 278
Size: 53 83 186 936 618 1714 2468
Fragments arranged by size:
2468 1714 936 618 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2702 3401 1937
Size: 765 699 4594
Fragments arranged by size:
4594 765 699
Acc65I G'GTAC_C
Cuts at: 3379 3379
Size: 6058
AclI AA'CG_TT
Cuts at: 1563 4185 4558 1563
Size: 2622 373 3063
Fragments arranged by size:
3063 2622 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4121 5505 5773 1632
Size: 2489 1384 268 1917
Fragments arranged by size:
2489 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5861
AflIII A'CryG_T
Cuts at: 1203 3240 4957 5934 1203
Size: 2037 1717 977 1327
Fragments arranged by size:
2037 1717 1327 977
AgeI A'CCGG_T
Cuts at: 4889 4889
Size: 6058
AhdI GACnn_n'nnGTC
Cuts at: 4787 4787
Size: 6058
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6058
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6058
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3227 3259 3227
Size: 32 6026
AlwNI CAG_nnn'CTG
Cuts at: 2444 3801 5373 2444
Size: 1357 1572 3129
Fragments arranged by size:
3129 1572 1357
ApaI G_GGCC'C
Cuts at: 3062 3062
Size: 6058
ApaLI G'TGCA_C
Cuts at: 4115 5271 4115
Size: 1156 4902
ApoI r'AATT_y
Cuts at: 1493 3367 3593 3745 5854 1493
Size: 1874 226 152 2109 1697
Fragments arranged by size:
2109 1874 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4612 160
Size: 872 3580 1606
Fragments arranged by size:
3580 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
AvaI C'yCGr_G
Cuts at: 1870 1943 2102 2190 2217 3383 1870
Size: 73 159 88 27 1166 4545
Fragments arranged by size:
4545 1166 159 88 73 27
BamHI G'GATC_C
Cuts at: 3021 3388 3755 3021
Size: 367 367 5324
Fragments arranged by size:
5324 367 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3344 3379 4834 618
Size: 325 217 627 1557 35 1455 1842
Fragments arranged by size:
1842 1557 1455 627 325 217 35
BanII G_rGCy'C
Cuts at: 1391 3062 3132 3377 1391
Size: 1671 70 245 4072
Fragments arranged by size:
4072 1671 245 70
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2870 928
Size: 971 971 4116
Fragments arranged by size:
4116 971 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4249 4283 1903
Size: 34 50 34 2228 34 3678
Fragments arranged by size:
3678 2228 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2496 3950 5160 2496
Size: 1454 1210 3394
Fragments arranged by size:
3394 1454 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6058
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2043 2106 4669
Size: 107 122 71 631 605 371 63 2563
Cuts at: 4669 136
Size: 1525
Fragments arranged by size:
2563 1525 631 605 371 122 107 71
63
BglII A'GATC_T
Cuts at: 6053 6053
Size: 6058
BlpI GC'TnA_GC
Cuts at: 3475 3475
Size: 6058
Bme1580I G_kGCm'C
Cuts at: 1790 2138 3062 3347 4119 5275 1790
Size: 348 924 285 772 1156 2573
Fragments arranged by size:
2573 1156 924 772 348 285
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6058
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2323 2459 3789
Size: 416 53 83 399 24 906 136 1330
Cuts at: 3789 4747 442
Size: 958 1753
Fragments arranged by size:
1753 1330 958 906 416 399 136 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6058
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3111 3111
Size: 6058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3239 4718 1239
Size: 2000 1479 2579
Fragments arranged by size:
2579 2000 1479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2564 4183 5048 5346 2564
Size: 1619 865 298 3276
Fragments arranged by size:
3276 1619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2925 4721 882
Size: 617 1426 1796 2219
Fragments arranged by size:
2219 1796 1426 617
BsaAI yAC'GTr
Cuts at: 493 1204 3849 493
Size: 711 2645 2702
Fragments arranged by size:
2702 2645 711
BsaBI GATnn'nnATC
Cuts at: 6052 6052
Size: 6058
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2151 3865 4247 4882
Size: 53 83 186 936 618 1714 382 635
Cuts at: 4882 275
Size: 1451
Fragments arranged by size:
1714 1451 936 635 618 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2637 4491 4889 5163 5310 5721 2637
Size: 1854 398 274 147 411 2974
Fragments arranged by size:
2974 1854 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3227 3257 3320 3350 1178
Size: 30 165 30 1824 30 63 30 3886
Fragments arranged by size:
3886 1824 165 63 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2310 2313 2561 3148 1186
Size: 946 178 3 248 587 4096
Fragments arranged by size:
4096 946 587 248 178 3
BseYI C'CCAG_C
Cuts at: 2429 5261 5757 2429
Size: 2832 496 2730
Fragments arranged by size:
2832 2730 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2522 2642 3308 1163
Size: 573 786 120 666 3913
Fragments arranged by size:
3913 786 666 573 120
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3508 4269 5297 1540
Size: 275 186 1507 761 1028 2301
Fragments arranged by size:
2301 1507 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2181 4119 4204 5275 2181
Size: 1938 85 1071 2964
Fragments arranged by size:
2964 1938 1071 85
BsmI GAATG_Cn'
Cuts at: 2602 3569 3662 2602
Size: 967 93 4998
Fragments arranged by size:
4998 967 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 2181 3062 3132 3347 3377 4119
Size: 399 348 43 881 70 215 30 742
Cuts at: 4119 4204 5275 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 881 742 399 348 215 85
70 43 30
BspEI T'CCGG_A
Cuts at: 5721 5721
Size: 6058
BspHI T'CATG_A
Cuts at: 2811 2883 3132 3947 5677 2811
Size: 72 249 815 1730 3192
Fragments arranged by size:
3192 1730 815 249 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3111 3346 3413 844
Size: 2267 235 67 3489
Fragments arranged by size:
3489 2267 235 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3945 1394
Size: 429 2122 3507
Fragments arranged by size:
3507 2122 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2424 4553 4727 65
Size: 1183 1176 2129 174 1396
Fragments arranged by size:
2129 1396 1183 1176 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2816 4702 4889 1700
Size: 110 1006 1886 187 2869
Fragments arranged by size:
2869 1886 1006 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6058
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6058
BssSI C'ACGA_G
Cuts at: 4118 5130 4118
Size: 1012 5046
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6058
BstBI TT'CG_AA
Cuts at: 4862 4862
Size: 6058
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6058
BstXI CCAn_nnnn'nTGG
Cuts at: 2875 2875
Size: 6058
BstYI r'GATC_y
Cuts at: 1972 2343 3021 3388 3755 4140 4157 5609 5695
Size: 371 678 367 367 385 17 1452 86
Cuts at: 5695 5707 6053 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 678 385 371 367 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2795 2868 3053 513
Size: 552 1730 73 185 3518
Fragments arranged by size:
3518 1730 552 185 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2556 2758 2941 487
Size: 683 56 1330 202 183 3604
Fragments arranged by size:
3604 1330 683 202 183 56
BtsI GCAGTG_nn'
Cuts at: 2947 3574 4368 4388 2947
Size: 627 794 20 4617
Fragments arranged by size:
4617 794 627 20
ClaI AT'CG_AT
Cuts at: 1554 3300 1554
Size: 1746 4312
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6023
DraI TTT'AAA
Cuts at: 3362 3362
Size: 6058
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5065 809
Size: 507 3749 1802
Fragments arranged by size:
3749 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2850 3039 3138
Size: 54 1425 50 275 18 1020 189 99
Cuts at: 3138 3315 3323 3505 4394 6023 8
Size: 177 8 182 889 1629 43
Fragments arranged by size:
1629 1425 1020 889 275 189 182 177
99 54 50 43 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 3505 1537
Size: 275 1693 4090
Fragments arranged by size:
4090 1693 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3170 3988 1353
Size: 1817 818 3423
Fragments arranged by size:
3423 1817 818
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4645 5019 5165 387
Size: 1064 138 3056 374 146 1280
Fragments arranged by size:
3056 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3239 4121 4718 5505 5773 1239
Size: 393 1607 882 597 787 268 1524
Fragments arranged by size:
1607 1524 882 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3058 3059 3058
Size: 1 6057
EcoRI G'AATT_C
Cuts at: 3367 3745 3367
Size: 378 5680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2170 3271 2170
Size: 1101 4957
FspI TGC'GCA
Cuts at: 4564 4564
Size: 6058
HaeII r_GCGC'y
Cuts at: 1678 5205 1678
Size: 3527 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2328 2360 3163 3195 3320
Size: 32 135 32 756 32 803 32 125
Cuts at: 3320 3352 4699 4731 4773 4805 1373
Size: 32 1347 32 42 32 2626
Fragments arranged by size:
2626 1347 803 756 135 125 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3402 3648 669
Size: 988 281 1464 246 3079
Fragments arranged by size:
3079 1464 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6058
HpaI GTT'AAC
Cuts at: 3648 3648
Size: 6058
KpnI G_GTAC'C
Cuts at: 3383 3383
Size: 6058
MfeI C'AATT_G
Cuts at: 3657 3657
Size: 6058
MluI A'CGCG_T
Cuts at: 5934 5934
Size: 6058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2265 5172 5356 1305
Size: 960 2907 184 2007
Fragments arranged by size:
2907 2007 960 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2852 3041 3140 10
Size: 54 1425 343 1020 189 99 2928
Fragments arranged by size:
2928 1425 1020 343 189 99 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3074 4016 4375 4534 518
Size: 648 1908 942 359 159 2042
Fragments arranged by size:
2042 1908 942 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2077 2322 2695 3126 3474 4151 5299 5544
Size: 471 245 373 431 348 677 1148 245
Cuts at: 5544 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 471 431 373 348 245
245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5506
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6058
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6058
NotI GC'GGCC_GC
Cuts at: 3505 3505
Size: 6058
NspI r_CATG'y
Cuts at: 1132 1229 3244 3416 4961 1132
Size: 97 2015 172 1545 2229
Fragments arranged by size:
2229 2015 1545 172 97
PasI CC'CwG_GG
Cuts at: 1348 2332 1348
Size: 984 5074
PciI A'CATG_T
Cuts at: 3240 4957 3240
Size: 1717 4341
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2848 1108
Size: 1740 4318
PfoI T'CCnGG_A
Cuts at: 2874 3024 3216 2874
Size: 150 192 5716
Fragments arranged by size:
5716 192 150
PmeI GTTT'AAAC
Cuts at: 3362 3362
Size: 6058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3227 3259 4127 4159 5640 5672 3227
Size: 32 868 32 1481 32 3613
Fragments arranged by size:
3613 1481 868 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2870 1613
Size: 1257 4801
PsiI TTA'TAA
Cuts at: 3628 3628
Size: 6058
PspOMI G'GGCC_C
Cuts at: 3058 3058
Size: 6058
PstI C_TGCA'G
Cuts at: 830 2901 3410 830
Size: 2071 509 3478
Fragments arranged by size:
3478 2071 509
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6058
PvuII CAG'CTG
Cuts at: 2077 2695 2077
Size: 618 5440
SalI G'TCGA_C
Cuts at: 1936 3400 1936
Size: 1464 4594
SapI GCTCTTCn'nnn_
Cuts at: 3170 3170
Size: 6058
SbfI CC_TGCA'GG
Cuts at: 3410 3410
Size: 6058
ScaI AGT'ACT
Cuts at: 4306 6018 4306
Size: 1712 4346
SfcI C'TryA_G
Cuts at: 826 1045 2027 2897 3406 4541 5222 5413 826
Size: 219 982 870 509 1135 681 191 1471
Fragments arranged by size:
1471 1135 982 870 681 509 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6058
SmaI CCC'GGG
Cuts at: 1872 2192 2219 3385 1872
Size: 320 27 1166 4545
Fragments arranged by size:
4545 1166 320 27
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2579 4162 5063 5325 820
Size: 197 926 636 1583 901 262 1553
Fragments arranged by size:
1583 1553 926 901 636 262 197
SnaBI TAC'GTA
Cuts at: 493 3849 493
Size: 3356 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6058
SphI G_CATG'C
Cuts at: 1132 3416 1132
Size: 2284 3774
SspI AAT'ATT
Cuts at: 5 52 3982 5
Size: 47 3930 2081
Fragments arranged by size:
3930 2081 47
StuI AGG'CCT
Cuts at: 1517 3212 1517
Size: 1695 4363
StyI C'CwwG_G
Cuts at: 513 1065 3497 513
Size: 552 2432 3074
Fragments arranged by size:
3074 2432 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3011 3362 4085 4102 4255 4440 4885
Size: 1255 1431 351 723 17 153 185 445
Cuts at: 4885 325
Size: 1498
Fragments arranged by size:
1498 1431 1255 723 445 351 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4304 6016 96
Size: 274 80 33 51 1443 2327 1712 138
Fragments arranged by size:
2327 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6018 6050 6018
Size: 32 6026
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6058
XbaI T'CTAG_A
Cuts at: 2398 3394 2398
Size: 996 5062
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2911 3073 2911
Size: 162 5896
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6058
XmaI C'CCGG_G
Cuts at: 1870 2190 2217 3383 1870
Size: 320 27 1166 4545
Fragments arranged by size:
4545 1166 320 27
XmnI GAAnn'nnTTC
Cuts at: 4187 4187
Size: 6058
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2152 3866 276
Size: 53 83 186 936 618 1714 2468
Fragments arranged by size:
2468 1714 936 618 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI