Restriction map for
pFN21AE4431
(Circular) (Six-base) MAPSORT of: pFN21AE4431.seq Check: 1059 from: 1 to: 6055
pFN21AE4431 6055 bp
With 182 enzymes: SgfI *
March 28, 2012 14:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2032 2032
Size: 6055
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2024 3865 278
Size: 53 83 186 936 488 1841 2468
Fragments arranged by size:
2468 1841 936 488 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3398 1937
Size: 1461 4594
Acc65I G'GTAC_C
Cuts at: 3376 3376
Size: 6055
AclI AA'CG_TT
Cuts at: 1563 4182 4555 1563
Size: 2619 373 3063
Fragments arranged by size:
3063 2619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2372 2765 2816 2851 4118 5502 5770 1632
Size: 740 393 51 35 1267 1384 268 1917
Fragments arranged by size:
1917 1384 1267 740 393 268 51 35
AfeI AGC'GCT
Cuts at: 2063 2063
Size: 6055
AflII C'TTAA_G
Cuts at: 820 1017 3311 820
Size: 197 2294 3564
Fragments arranged by size:
3564 2294 197
AflIII A'CryG_T
Cuts at: 1203 3167 4954 5931 1203
Size: 1964 1787 977 1327
Fragments arranged by size:
1964 1787 1327 977
AgeI A'CCGG_T
Cuts at: 4886 4886
Size: 6055
AhdI GACnn_n'nnGTC
Cuts at: 2354 2380 4784 2354
Size: 26 2404 3625
Fragments arranged by size:
3625 2404 26
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6055
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6055
AlwNI CAG_nnn'CTG
Cuts at: 2077 2596 2725 3798 5370 2077
Size: 519 129 1073 1572 2762
Fragments arranged by size:
2762 1572 1073 519 129
ApaLI G'TGCA_C
Cuts at: 4112 5268 4112
Size: 1156 4899
ApoI r'AATT_y
Cuts at: 1493 2708 2975 3364 3590 3742 5851 1493
Size: 1215 267 389 226 152 2109 1697
Fragments arranged by size:
2109 1697 1215 389 267 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2913 4609 160
Size: 872 1881 1696 1606
Fragments arranged by size:
1881 1696 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
AvaI C'yCGr_G
Cuts at: 1870 1943 2639 3380 1870
Size: 73 696 741 4545
Fragments arranged by size:
4545 741 696 73
BamHI G'GATC_C
Cuts at: 3385 3752 3385
Size: 367 5688
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2324 3376 4831 618
Size: 325 217 627 537 1052 1455 1842
Fragments arranged by size:
1842 1455 1052 627 537 325 217
BanII G_rGCy'C
Cuts at: 1391 3374 1391
Size: 1983 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2353 2607 3170 3222 928
Size: 971 454 254 563 52 3761
Fragments arranged by size:
3761 971 563 454 254 52
BbvCI CC'TCA_GC
Cuts at: 2339 2655 2339
Size: 316 5739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4246 4280 1903
Size: 34 2309 34 3678
Fragments arranged by size:
3678 2309 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3947 5157 3947
Size: 1210 4845
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6055
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4666 136
Size: 107 122 71 631 605 2994 1525
Fragments arranged by size:
2994 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2505 6050 2505
Size: 3545 2510
BlpI GC'TnA_GC
Cuts at: 3472 3472
Size: 6055
Bme1580I G_kGCm'C
Cuts at: 1790 4116 5272 1790
Size: 2326 1156 2573
Fragments arranged by size:
2573 2326 1156
BmgBI CAC'GTC
Cuts at: 1318 2605 1318
Size: 1287 4768
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2370 2880 3250
Size: 416 53 83 399 24 953 510 370
Cuts at: 3250 3786 4744 442
Size: 536 958 1753
Fragments arranged by size:
1753 958 953 536 510 416 399 370
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6055
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 6055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2747 3041 4715 1239
Size: 1508 294 1674 2579
Fragments arranged by size:
2579 1674 1508 294
Bpu10I CC'TnA_GC
Cuts at: 2339 2655 2339
Size: 316 5739
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3275 4180 5045 5343 3275
Size: 905 865 298 3987
Fragments arranged by size:
3987 905 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2165 4718 882
Size: 617 666 2553 2219
Fragments arranged by size:
2553 2219 666 617
BsaAI yAC'GTr
Cuts at: 493 1204 3846 493
Size: 711 2642 2702
Fragments arranged by size:
2702 2642 711
BsaBI GATnn'nnATC
Cuts at: 2400 6049 2400
Size: 3649 2406
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 3862 4244 4879
Size: 53 83 186 936 488 1841 382 635
Cuts at: 4879 275
Size: 1451
Fragments arranged by size:
1841 1451 936 635 488 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4488 4886 5160 5307 5718 4488
Size: 398 274 147 411 4825
Fragments arranged by size:
4825 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3038 3068 1178
Size: 30 165 30 1635 30 4165
Fragments arranged by size:
4165 1635 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2211 2216 2588 2591 3284 1186
Size: 1025 5 372 3 693 3957
Fragments arranged by size:
3957 1025 693 372 5 3
BseYI C'CCAG_C
Cuts at: 2257 2872 5258 5754 2257
Size: 615 2386 496 2558
Fragments arranged by size:
2558 2386 615 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3152 1163
Size: 573 1416 4066
Fragments arranged by size:
4066 1416 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3505 4266 5294 1540
Size: 275 186 1504 761 1028 2301
Fragments arranged by size:
2301 1504 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4116 4201 5272 4116
Size: 85 1071 4899
Fragments arranged by size:
4899 1071 85
BsmI GAATG_Cn'
Cuts at: 2426 3566 3659 2426
Size: 1140 93 4822
Fragments arranged by size:
4822 1140 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3374 4116 4201 5272 1391
Size: 399 1584 742 85 1071 2174
Fragments arranged by size:
2174 1584 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5718 5718
Size: 6055
BspHI T'CATG_A
Cuts at: 3944 5674 3944
Size: 1730 4325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2032 3410 844
Size: 1188 1378 3489
Fragments arranged by size:
3489 1378 1188
BsrBI CCG'CTC
Cuts at: 1394 1823 3942 1394
Size: 429 2119 3507
Fragments arranged by size:
3507 2119 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3114 4550 4724 65
Size: 1183 1866 1436 174 1396
Fragments arranged by size:
1866 1436 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4699 4886 1700
Size: 110 2889 187 2869
Fragments arranged by size:
2889 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6055
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6055
BssSI C'ACGA_G
Cuts at: 2089 4115 5127 2089
Size: 2026 1012 3017
Fragments arranged by size:
3017 2026 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6055
BstBI TT'CG_AA
Cuts at: 4859 4859
Size: 6055
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6055
BstXI CCAn_nnnn'nTGG
Cuts at: 2965 2965
Size: 6055
BstYI r'GATC_y
Cuts at: 1972 2100 2505 3385 3752 4137 4154 5606 5692
Size: 128 405 880 367 385 17 1452 86
Cuts at: 5692 5704 6050 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 880 405 385 367 346 128
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2328 2895 513
Size: 552 939 324 567 3673
Fragments arranged by size:
3673 939 567 552 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5316
Fragments arranged by size:
5316 683 56
BtsI GCAGTG_nn'
Cuts at: 3235 3281 3571 4365 4385 3235
Size: 46 290 794 20 4905
Fragments arranged by size:
4905 794 290 46 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6055
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6020
DraI TTT'AAA
Cuts at: 2713 3359 2713
Size: 646 5409
DraIII CAC_nnn'GTG
Cuts at: 2414 2414
Size: 6055
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5062 809
Size: 507 3746 1802
Fragments arranged by size:
3746 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2955 3502 4391
Size: 54 1425 50 275 18 1125 547 889
Cuts at: 4391 6020 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1125 889 547 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3502 1537
Size: 275 1690 4090
Fragments arranged by size:
4090 1690 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2178 2741 3141 3985 1353
Size: 825 563 400 844 3423
Fragments arranged by size:
3423 844 825 563 400
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4642 5016 5162 387
Size: 1064 138 3053 374 146 1280
Fragments arranged by size:
3053 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2372 2747 2765 2816 2851 3041 4118
Size: 393 740 375 18 51 35 190 1077
Cuts at: 4118 4715 5502 5770 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1077 787 740 597 393 375 268
190 51 35 18
EcoO109I rG'GnC_Cy
Cuts at: 2365 2365
Size: 6055
EcoRI G'AATT_C
Cuts at: 3364 3742 3364
Size: 378 5677
FspI TGC'GCA
Cuts at: 4561 4561
Size: 6055
HaeII r_GCGC'y
Cuts at: 1678 2065 5202 1678
Size: 387 3137 2531
Fragments arranged by size:
3137 2531 387
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2222 2254 2340 2366 2372
Size: 32 135 32 650 32 86 26 6
Cuts at: 2372 2398 2656 2688 3271 3303 4696 4728 4770
Size: 26 258 32 583 32 1393 32 42
Cuts at: 4770 4802 1373
Size: 32 2626
Fragments arranged by size:
2626 1393 650 583 258 135 86 42
32 32 32 32 32 32 32 26
26 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3399 3645 669
Size: 988 281 1461 246 3079
Fragments arranged by size:
3079 1461 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6055
HpaI GTT'AAC
Cuts at: 3645 3645
Size: 6055
KpnI G_GTAC'C
Cuts at: 3380 3380
Size: 6055
MfeI C'AATT_G
Cuts at: 3654 3654
Size: 6055
MluI A'CGCG_T
Cuts at: 5931 5931
Size: 6055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2049 5169 5353 1305
Size: 744 3120 184 2007
Fragments arranged by size:
3120 2007 744 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2957 10
Size: 54 1425 343 1125 3108
Fragments arranged by size:
3108 1425 1125 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2042 4013 4372 4531 518
Size: 648 876 1971 359 159 2042
Fragments arranged by size:
2042 1971 876 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2446 2725 2785 3471 4148 5296 5541 1606
Size: 840 279 60 686 677 1148 245 2120
Fragments arranged by size:
2120 1148 840 686 677 279 245 60
NcoI C'CATG_G
Cuts at: 513 1065 2004 2328 513
Size: 552 939 324 4240
Fragments arranged by size:
4240 939 552 324
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6055
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6055
NotI GC'GGCC_GC
Cuts at: 3502 3502
Size: 6055
NspI r_CATG'y
Cuts at: 1132 1229 3413 4958 1132
Size: 97 2184 1545 2229
Fragments arranged by size:
2229 2184 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6055
PciI A'CATG_T
Cuts at: 4954 4954
Size: 6055
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2596 2953 3109 1108
Size: 1488 357 156 4054
Fragments arranged by size:
4054 1488 357 156
PfoI T'CCnGG_A
Cuts at: 2103 2121 2589 3120 2103
Size: 18 468 531 5038
Fragments arranged by size:
5038 531 468 18
PmeI GTTT'AAAC
Cuts at: 3359 3359
Size: 6055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4124 4156 5637 5669 4124
Size: 32 1481 32 4510
Fragments arranged by size:
4510 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2365 2365
Size: 6055
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6055
PsiI TTA'TAA
Cuts at: 3348 3625 3348
Size: 277 5778
PstI C_TGCA'G
Cuts at: 830 2069 3407 830
Size: 1239 1338 3478
Fragments arranged by size:
3478 1338 1239
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6055
PvuII CAG'CTG
Cuts at: 2446 2725 2785 2446
Size: 279 60 5716
Fragments arranged by size:
5716 279 60
SalI G'TCGA_C
Cuts at: 1936 3397 1936
Size: 1461 4594
SbfI CC_TGCA'GG
Cuts at: 3407 3407
Size: 6055
ScaI AGT'ACT
Cuts at: 4303 6015 4303
Size: 1712 4343
SfcI C'TryA_G
Cuts at: 826 1045 2065 3184 3403 4538 5219 5410 826
Size: 219 1020 1119 219 1135 681 191 1471
Fragments arranged by size:
1471 1135 1119 1020 681 219 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6055
SmaI CCC'GGG
Cuts at: 1872 3382 1872
Size: 1510 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2639 3290 3311 4159 5060 5322
Size: 197 926 696 651 21 848 901 262
Cuts at: 5322 820
Size: 1553
Fragments arranged by size:
1553 926 901 848 696 651 262 197
21
SnaBI TAC'GTA
Cuts at: 493 3846 493
Size: 3353 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6055
SphI G_CATG'C
Cuts at: 1132 3413 1132
Size: 2281 3774
SspI AAT'ATT
Cuts at: 5 52 3979 5
Size: 47 3927 2081
Fragments arranged by size:
3927 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6055
StyI C'CwwG_G
Cuts at: 513 1065 2004 2328 3494 513
Size: 552 939 324 1166 3074
Fragments arranged by size:
3074 1166 939 552 324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2421 3319 4082 4099 4252 4437 4882
Size: 1255 841 898 763 17 153 185 445
Cuts at: 4882 325
Size: 1498
Fragments arranged by size:
1498 1255 898 841 763 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4301 6013 96
Size: 274 80 33 51 1443 2324 1712 138
Fragments arranged by size:
2324 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6015 6047 6015
Size: 32 6023
Tth111I GACn'n_nGTC
Cuts at: 1316 2111 1316
Size: 795 5260
XbaI T'CTAG_A
Cuts at: 3391 3391
Size: 6055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2893 2893
Size: 6055
XhoI C'TCGA_G
Cuts at: 1943 2639 1943
Size: 696 5359
XmaI C'CCGG_G
Cuts at: 1870 3380 1870
Size: 1510 4545
XmnI GAAnn'nnTTC
Cuts at: 3010 4184 3010
Size: 1174 4881
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2022 3863 276
Size: 53 83 186 936 488 1841 2468
Fragments arranged by size:
2468 1841 936 488 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI