Restriction map for
pFN21AE4430
(Circular) (Six-base) MAPSORT of: pFN21AE4430.seq Check: 9235 from: 1 to: 6049
pFN21AE4430 6049 bp
With 182 enzymes: SgfI *
March 28, 2012 13:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2976 3859 278
Size: 53 83 186 936 1440 883 2468
Fragments arranged by size:
2468 1440 936 883 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3392 1937
Size: 1455 4594
Acc65I G'GTAC_C
Cuts at: 3370 3370
Size: 6049
AclI AA'CG_TT
Cuts at: 1563 4176 4549 1563
Size: 2613 373 3063
Fragments arranged by size:
3063 2613 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2575 3237 4112 5496 5764 1632
Size: 943 662 875 1384 268 1917
Fragments arranged by size:
1917 1384 943 875 662 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5852
AflIII A'CryG_T
Cuts at: 1203 4948 5925 1203
Size: 3745 977 1327
Fragments arranged by size:
3745 1327 977
AgeI A'CCGG_T
Cuts at: 4880 4880
Size: 6049
AhdI GACnn_n'nnGTC
Cuts at: 4778 4778
Size: 6049
AleI CACnn'nnGTG
Cuts at: 1166 2631 1166
Size: 1465 4584
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6049
AlwNI CAG_nnn'CTG
Cuts at: 2114 2717 2915 2969 3792 5364 2114
Size: 603 198 54 823 1572 2799
Fragments arranged by size:
2799 1572 823 603 198 54
ApaI G_GGCC'C
Cuts at: 2054 2079 2054
Size: 25 6024
ApaLI G'TGCA_C
Cuts at: 2567 4106 5262 2567
Size: 1539 1156 3354
Fragments arranged by size:
3354 1539 1156
ApoI r'AATT_y
Cuts at: 1493 2540 3149 3358 3584 3736 5845 1493
Size: 1047 609 209 226 152 2109 1697
Fragments arranged by size:
2109 1697 1047 609 226 209 152
AseI AT'TA_AT
Cuts at: 160 1032 4603 160
Size: 872 3571 1606
Fragments arranged by size:
3571 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2169 3374 1870
Size: 73 93 133 1205 4545
Fragments arranged by size:
4545 1205 133 93 73
AvrII C'CTAG_G
Cuts at: 3107 3107
Size: 6049
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2281 2314 2281
Size: 33 6016
BamHI G'GATC_C
Cuts at: 2660 3379 3746 2660
Size: 719 367 4963
Fragments arranged by size:
4963 719 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3370 4825 618
Size: 325 217 627 1583 1455 1842
Fragments arranged by size:
1842 1583 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2054 2079 2235 2450 2524 2649 2931 3053
Size: 663 25 156 215 74 125 282 122
Cuts at: 3053 3321 3368 1391
Size: 268 47 4072
Fragments arranged by size:
4072 663 282 268 215 156 125 122
74 47 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3069 928
Size: 971 1170 3908
Fragments arranged by size:
3908 1170 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4240 4274 1903
Size: 34 2303 34 3678
Fragments arranged by size:
3678 2303 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3941 5151 3941
Size: 1210 4839
BclI T'GATC_A
Cuts at: 1548 2118 2469 1548
Size: 570 351 5128
Fragments arranged by size:
5128 570 351
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2316 3338 4660
Size: 107 122 71 631 605 644 1022 1322
Cuts at: 4660 136
Size: 1525
Fragments arranged by size:
1525 1322 1022 644 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6044 6044
Size: 6049
BlpI GC'TnA_GC
Cuts at: 2103 2523 3315 3466 2103
Size: 420 792 151 4686
Fragments arranged by size:
4686 792 420 151
Bme1580I G_kGCm'C
Cuts at: 1790 2054 2079 2571 4110 5266 1790
Size: 264 25 492 1539 1156 2573
Fragments arranged by size:
2573 1539 1156 492 264 25
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6049
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2325 2625 2785
Size: 416 53 83 399 24 908 300 160
Cuts at: 2785 3315 3780 4738 442
Size: 530 465 958 1753
Fragments arranged by size:
1753 958 908 530 465 416 399 300
160 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6049
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 6049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2249 2363 2663 2892 3085 3294 4709
Size: 944 66 114 300 229 193 209 1415
Cuts at: 4709 1239
Size: 2579
Fragments arranged by size:
2579 1415 944 300 229 209 193 114
66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4174 5039 5337 4174
Size: 865 298 4886
Fragments arranged by size:
4886 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2809 4712 882
Size: 617 1310 1903 2219
Fragments arranged by size:
2219 1903 1310 617
BsaAI yAC'GTr
Cuts at: 493 1204 3840 493
Size: 711 2636 2702
Fragments arranged by size:
2702 2636 711
BsaBI GATnn'nnATC
Cuts at: 6043 6043
Size: 6049
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2854 2973 3856 4238
Size: 53 83 186 936 1321 119 883 382
Cuts at: 4238 4873 275
Size: 635 1451
Fragments arranged by size:
1451 1321 936 883 635 382 186 119
83 53
BsaWI w'CCGG_w
Cuts at: 4482 4880 5154 5301 5712 4482
Size: 398 274 147 411 4819
Fragments arranged by size:
4819 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2591 2621 3268 3298 1178
Size: 30 165 30 1188 30 647 30 3929
Fragments arranged by size:
3929 1188 647 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2816 2819 2828 2831 2965 3117 3276 1186
Size: 1630 3 9 3 134 152 159 3959
Fragments arranged by size:
3959 1630 159 152 134 9 3 3
BseYI C'CCAG_C
Cuts at: 2124 2200 2962 5252 5748 2124
Size: 76 762 2290 496 2425
Fragments arranged by size:
2425 2290 762 496 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3233 1163
Size: 573 1497 3979
Fragments arranged by size:
3979 1497 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3499 4260 5288 1540
Size: 275 186 1498 761 1028 2301
Fragments arranged by size:
2301 1498 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2517 2524 2571 2649 4110 4195 5266 2517
Size: 7 47 78 1461 85 1071 3300
Fragments arranged by size:
3300 1461 1071 85 78 47 7
BsmI GAATG_Cn'
Cuts at: 3560 3653 3560
Size: 93 5956
BsmBI CGTCTCn'nnnn_
Cuts at: 2704 2704
Size: 6049
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2079 2235 2450 2517 2524 2571
Size: 399 264 25 156 215 67 7 47
Cuts at: 2571 2649 2931 3053 3321 3368 4110 4195 5266
Size: 78 282 122 268 47 742 85 1071
Cuts at: 5266 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 282 268 264 215
156 122 85 78 67 47 47 25
7
BspEI T'CCGG_A
Cuts at: 5712 5712
Size: 6049
BspHI T'CATG_A
Cuts at: 3938 5668 3938
Size: 1730 4319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2625 3404 844
Size: 1781 779 3489
Fragments arranged by size:
3489 1781 779
BsrBI CCG'CTC
Cuts at: 1394 1823 2479 3936 1394
Size: 429 656 1457 3507
Fragments arranged by size:
3507 1457 656 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4544 4718 65
Size: 1183 3296 174 1396
Fragments arranged by size:
3296 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4693 4880 1700
Size: 110 2883 187 2869
Fragments arranged by size:
2883 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6049
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6049
BssSI C'ACGA_G
Cuts at: 4109 5121 4109
Size: 1012 5037
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6049
BstBI TT'CG_AA
Cuts at: 4853 4853
Size: 6049
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6049
BstXI CCAn_nnnn'nTGG
Cuts at: 2208 2208
Size: 6049
BstYI r'GATC_y
Cuts at: 1972 2660 3379 3746 4131 4148 5600 5686 5698
Size: 688 719 367 385 17 1452 86 12
Cuts at: 5698 6044 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 719 688 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2410 2718 3018 2410
Size: 308 300 5441
Fragments arranged by size:
5441 308 300
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5497
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5310
Fragments arranged by size:
5310 683 56
BtsI GCAGTG_nn'
Cuts at: 3081 3565 4359 4379 3081
Size: 484 794 20 4751
Fragments arranged by size:
4751 794 484 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6049
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6014
DraI TTT'AAA
Cuts at: 3353 3353
Size: 6049
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5056 809
Size: 507 3740 1802
Fragments arranged by size:
3740 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3339 3496 4385
Size: 54 1425 50 275 18 1509 157 889
Cuts at: 4385 6014 8
Size: 1629 43
Fragments arranged by size:
1629 1509 1425 889 275 157 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3496 1537
Size: 275 1684 4090
Fragments arranged by size:
4090 1684 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2837 3174 3979 1353
Size: 1484 337 805 3423
Fragments arranged by size:
3423 1484 805 337
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4636 5010 5156 387
Size: 1064 138 3047 374 146 1280
Fragments arranged by size:
3047 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2522 2647 2522
Size: 125 5924
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2249 2363 2575 2663 2892 3085
Size: 393 551 66 114 212 88 229 193
Cuts at: 3085 3237 3294 4112 4709 5496 5764 1239
Size: 152 57 818 597 787 268 1524
Fragments arranged by size:
1524 818 787 597 551 393 268 229
212 193 152 114 88 66 57
EcoO109I rG'GnC_Cy
Cuts at: 2051 2075 2076 2157 2577 2672 2679 2714 2941
Size: 24 1 81 420 95 7 35 227
Cuts at: 2941 3303 2051
Size: 362 4797
Fragments arranged by size:
4797 420 362 227 95 81 35 24
7 1
EcoRI G'AATT_C
Cuts at: 2540 3149 3358 3736 2540
Size: 609 209 378 4853
Fragments arranged by size:
4853 609 378 209
FspI TGC'GCA
Cuts at: 4555 4555
Size: 6049
HaeII r_GCGC'y
Cuts at: 1678 5196 1678
Size: 3518 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2241 2273 4690 4722 4764
Size: 32 135 32 669 32 2417 32 42
Cuts at: 4764 4796 1373
Size: 32 2626
Fragments arranged by size:
2626 2417 669 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3393 3639 669
Size: 988 281 1455 246 3079
Fragments arranged by size:
3079 1455 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6049
HpaI GTT'AAC
Cuts at: 3639 3639
Size: 6049
KpnI G_GTAC'C
Cuts at: 3374 3374
Size: 6049
MfeI C'AATT_G
Cuts at: 3648 3648
Size: 6049
MluI A'CGCG_T
Cuts at: 5925 5925
Size: 6049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2869 2923 5163 5347 1305
Size: 1564 54 2240 184 2007
Fragments arranged by size:
2240 2007 1564 184 54
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3341 10
Size: 54 1425 343 1509 2718
Fragments arranged by size:
2718 1509 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2631 4007 4366 4525 518
Size: 648 1465 1376 359 159 2042
Fragments arranged by size:
2042 1465 1376 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2124 2295 2708 2966 3314 3465 4142 5290
Size: 518 171 413 258 348 151 677 1148
Cuts at: 5290 5535 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 518 413 348 258 245
171 151
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5497
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6049
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6049
NotI GC'GGCC_GC
Cuts at: 3496 3496
Size: 6049
NspI r_CATG'y
Cuts at: 1132 1229 2068 3407 4952 1132
Size: 97 839 1339 1545 2229
Fragments arranged by size:
2229 1545 1339 839 97
PasI CC'CwG_GG
Cuts at: 1348 2208 1348
Size: 860 5189
PciI A'CATG_T
Cuts at: 4948 4948
Size: 6049
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2097 3143 1108
Size: 989 1046 4014
Fragments arranged by size:
4014 1046 989
PfoI T'CCnGG_A
Cuts at: 3153 3153
Size: 6049
PmeI GTTT'AAAC
Cuts at: 3353 3353
Size: 6049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4118 4150 5631 5663 4118
Size: 32 1481 32 4504
Fragments arranged by size:
4504 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2157 2577 2672 2157
Size: 420 95 5534
Fragments arranged by size:
5534 420 95
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6049
PsiI TTA'TAA
Cuts at: 3619 3619
Size: 6049
PspOMI G'GGCC_C
Cuts at: 2050 2075 2050
Size: 25 6024
PstI C_TGCA'G
Cuts at: 830 2713 3401 830
Size: 1883 688 3478
Fragments arranged by size:
3478 1883 688
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6049
PvuII CAG'CTG
Cuts at: 2124 2708 2966 3314 2124
Size: 584 258 348 4859
Fragments arranged by size:
4859 584 348 258
SacI G_AGCT'C
Cuts at: 2524 2649 2524
Size: 125 5924
SalI G'TCGA_C
Cuts at: 1936 3391 1936
Size: 1455 4594
SanDI GG'GwC_CC
Cuts at: 2157 2672 2157
Size: 515 5534
SbfI CC_TGCA'GG
Cuts at: 3401 3401
Size: 6049
ScaI AGT'ACT
Cuts at: 4297 6009 4297
Size: 1712 4337
SfcI C'TryA_G
Cuts at: 826 1045 2313 2365 2581 2709 2739 2945 3397
Size: 219 1268 52 216 128 30 206 452
Cuts at: 3397 4532 5213 5404 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1268 1135 681 452 219 216 206
191 128 52 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6049
SmaI CCC'GGG
Cuts at: 1872 3376 1872
Size: 1504 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4153 5054 5316 820
Size: 197 926 2210 901 262 1553
Fragments arranged by size:
2210 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3840 493
Size: 3347 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6049
SphI G_CATG'C
Cuts at: 1132 3407 1132
Size: 2275 3774
SspI AAT'ATT
Cuts at: 5 52 3973 5
Size: 47 3921 2081
Fragments arranged by size:
3921 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6049
StyI C'CwwG_G
Cuts at: 513 1065 2664 2931 3087 3107 3488 513
Size: 552 1599 267 156 20 381 3074
Fragments arranged by size:
3074 1599 552 381 267 156 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2075 2701 4076 4093 4246 4431 4876
Size: 1255 495 626 1375 17 153 185 445
Cuts at: 4876 325
Size: 1498
Fragments arranged by size:
1498 1375 1255 626 495 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4295 6007 96
Size: 274 80 33 51 1443 2318 1712 138
Fragments arranged by size:
2318 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2406 2438 2591 2623 6009 6041 2406
Size: 32 153 32 3386 32 2414
Fragments arranged by size:
3386 2414 153 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6049
XbaI T'CTAG_A
Cuts at: 3385 3385
Size: 6049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2338 2638 2338
Size: 300 5749
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6049
XmaI C'CCGG_G
Cuts at: 1870 3374 1870
Size: 1504 4545
XmnI GAAnn'nnTTC
Cuts at: 4178 4178
Size: 6049
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2974 3857 276
Size: 53 83 186 936 1440 883 2468
Fragments arranged by size:
2468 1440 936 883 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BbeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI
SrfI SwaI