Restriction map for
pFN21AE4424
(Circular) (Six-base) MAPSORT of: pFN21AE4424.seq Check: 3377 from: 1 to: 6022
pFN21AE4424 6022 bp
With 182 enzymes: SgfI *
March 28, 2012 13:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3832 278
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2535 3365 1937
Size: 598 830 4594
Fragments arranged by size:
4594 830 598
Acc65I G'GTAC_C
Cuts at: 3343 3343
Size: 6022
AclI AA'CG_TT
Cuts at: 1563 4149 4522 1563
Size: 2586 373 3063
Fragments arranged by size:
3063 2586 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4085 5469 5737 1632
Size: 2453 1384 268 1917
Fragments arranged by size:
2453 1917 1384 268
AfeI AGC'GCT
Cuts at: 2888 2888
Size: 6022
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5825
AflIII A'CryG_T
Cuts at: 1203 4921 5898 1203
Size: 3718 977 1327
Fragments arranged by size:
3718 1327 977
AgeI A'CCGG_T
Cuts at: 4853 4853
Size: 6022
AhdI GACnn_n'nnGTC
Cuts at: 4751 4751
Size: 6022
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6022
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2159 1184
Size: 975 5047
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2456 2488 2456
Size: 32 5990
AlwNI CAG_nnn'CTG
Cuts at: 2596 3765 5337 2596
Size: 1169 1572 3281
Fragments arranged by size:
3281 1572 1169
ApaI G_GGCC'C
Cuts at: 2688 3187 2688
Size: 499 5523
ApaLI G'TGCA_C
Cuts at: 4079 5235 4079
Size: 1156 4866
ApoI r'AATT_y
Cuts at: 1493 3331 3557 3709 5818 1493
Size: 1838 226 152 2109 1697
Fragments arranged by size:
2109 1838 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4576 160
Size: 872 3544 1606
Fragments arranged by size:
3544 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
AvaI C'yCGr_G
Cuts at: 1870 1943 2715 3037 3087 3347 1870
Size: 73 772 322 50 260 4545
Fragments arranged by size:
4545 772 322 260 73 50
BamHI G'GATC_C
Cuts at: 2652 3352 3719 2652
Size: 700 367 4955
Fragments arranged by size:
4955 700 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2327 2795 2879 3236 3343
Size: 325 217 627 540 468 84 357 107
Cuts at: 3343 4798 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 540 468 357 325 217
107 84
BanII G_rGCy'C
Cuts at: 1391 2202 2292 2688 3187 3249 3341 1391
Size: 811 90 396 499 62 92 4072
Fragments arranged by size:
4072 811 499 396 92 90 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2708 928
Size: 971 809 4242
Fragments arranged by size:
4242 971 809
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4213 4247 1903
Size: 34 2276 34 3678
Fragments arranged by size:
3678 2276 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3914 5124 3914
Size: 1210 4812
BclI T'GATC_A
Cuts at: 1548 2373 1548
Size: 825 5197
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4633 136
Size: 107 122 71 631 605 2961 1525
Fragments arranged by size:
2961 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6017 6017
Size: 6022
BlpI GC'TnA_GC
Cuts at: 3439 3439
Size: 6022
Bme1580I G_kGCm'C
Cuts at: 1790 2688 3187 4083 5239 1790
Size: 898 499 896 1156 2573
Fragments arranged by size:
2573 1156 898 896 499
BmgBI CAC'GTC
Cuts at: 1318 3139 1318
Size: 1821 4201
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3181 3753 4711
Size: 416 53 83 399 24 1764 572 958
Cuts at: 4711 442
Size: 1753
Fragments arranged by size:
1764 1753 958 572 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6022
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 6022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2507 2957 3299 4682 1239
Size: 1268 450 342 1383 2579
Fragments arranged by size:
2579 1383 1268 450 342
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2090 4147 5012 5310 2090
Size: 2057 865 298 2802
Fragments arranged by size:
2802 2057 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2442 4685 882
Size: 617 943 2243 2219
Fragments arranged by size:
2243 2219 943 617
BsaAI yAC'GTr
Cuts at: 493 1204 3813 493
Size: 711 2609 2702
Fragments arranged by size:
2702 2609 711
BsaBI GATnn'nnATC
Cuts at: 2162 2301 6016 2162
Size: 139 3715 2168
Fragments arranged by size:
3715 2168 139
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3829 4211 4846 275
Size: 53 83 186 936 2296 382 635 1451
Fragments arranged by size:
2296 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4455 4853 5127 5274 5685 4455
Size: 398 274 147 411 4792
Fragments arranged by size:
4792 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2258 2288 2330 2360 2439
Size: 30 165 30 855 30 42 30 79
Cuts at: 2439 2456 2469 2486 1178
Size: 17 13 17 4714
Fragments arranged by size:
4714 855 165 79 42 30 30 30
30 17 17 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 2135 2333 3020 3095 3164 1186
Size: 925 24 198 687 75 69 4044
Fragments arranged by size:
4044 925 687 198 75 69 24
BseYI C'CCAG_C
Cuts at: 2766 2906 5225 5721 2766
Size: 140 2319 496 3067
Fragments arranged by size:
3067 2319 496 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2795 2942 3148 3305 1163
Size: 573 1059 147 206 157 3880
Fragments arranged by size:
3880 1059 573 206 157 147
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 3472 4233 5261 1540
Size: 275 186 60 1411 761 1028 2301
Fragments arranged by size:
2301 1411 1028 761 275 186 60
BsiHKAI G_wGCw'C
Cuts at: 2106 2202 2292 3249 4083 4168 5239 2106
Size: 96 90 957 834 85 1071 2889
Fragments arranged by size:
2889 1071 957 834 96 90 85
BsmI GAATG_Cn'
Cuts at: 3533 3626 3533
Size: 93 5929
BsmBI CGTCTCn'nnnn_
Cuts at: 2483 2483
Size: 6022
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2202 2292 2688 3187 3249 3341
Size: 399 316 96 90 396 499 62 92
Cuts at: 3341 4083 4168 5239 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 499 399 396 316 96
92 90 85 62
BspEI T'CCGG_A
Cuts at: 5685 5685
Size: 6022
BspHI T'CATG_A
Cuts at: 2166 3911 5641 2166
Size: 1745 1730 2547
Fragments arranged by size:
2547 1745 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3377 844
Size: 2533 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3036 3909 1394
Size: 429 1213 873 3507
Fragments arranged by size:
3507 1213 873 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4517 4691 65
Size: 1183 3269 174 1396
Fragments arranged by size:
3269 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4666 4853 1700
Size: 110 2856 187 2869
Fragments arranged by size:
2869 2856 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6022
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6022
BssSI C'ACGA_G
Cuts at: 3254 4082 5094 3254
Size: 828 1012 4182
Fragments arranged by size:
4182 1012 828
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6022
BstBI TT'CG_AA
Cuts at: 4826 4826
Size: 6022
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6022
BstXI CCAn_nnnn'nTGG
Cuts at: 2957 2957
Size: 6022
BstYI r'GATC_y
Cuts at: 1972 2586 2652 3105 3352 3719 4104 4121 5573
Size: 614 66 453 247 367 385 17 1452
Cuts at: 5573 5659 5671 6017 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 614 453 385 367 346 247
86 66 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2115 2526 2547 3240 513
Size: 552 1050 411 21 693 3295
Fragments arranged by size:
3295 1050 693 552 411 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5283
Fragments arranged by size:
5283 683 56
BtsI GCAGTG_nn'
Cuts at: 3224 3538 4332 4352 3224
Size: 314 794 20 4894
Fragments arranged by size:
4894 794 314 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6022
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2988 3023 529
Size: 35 2424 35 3528
Fragments arranged by size:
3528 2424 35 35
DraI TTT'AAA
Cuts at: 3326 3326
Size: 6022
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5029 809
Size: 507 3713 1802
Fragments arranged by size:
3713 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2826 3469 4358
Size: 54 1425 50 275 18 996 643 889
Cuts at: 4358 5987 8
Size: 1629 43
Fragments arranged by size:
1629 1425 996 889 643 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3469 1537
Size: 275 1657 4090
Fragments arranged by size:
4090 1657 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 3952 1353
Size: 812 1787 3423
Fragments arranged by size:
3423 1787 812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4609 4983 5129 387
Size: 1064 138 3020 374 146 1280
Fragments arranged by size:
3020 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2200 2290 3247 2200
Size: 90 957 4975
Fragments arranged by size:
4975 957 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2507 2957 3299 4085 4682 5469 5737
Size: 393 875 450 342 786 597 787 268
Cuts at: 5737 1239
Size: 1524
Fragments arranged by size:
1524 875 787 786 597 450 393 342
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2664 2828 2664
Size: 164 5858
EcoO109I rG'GnC_Cy
Cuts at: 2685 3183 2685
Size: 498 5524
EcoRI G'AATT_C
Cuts at: 3331 3709 3331
Size: 378 5644
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 6022
FspI TGC'GCA
Cuts at: 3230 4528 3230
Size: 1298 4724
HaeII r_GCGC'y
Cuts at: 1678 2843 2890 5169 1678
Size: 1165 47 2279 2531
Fragments arranged by size:
2531 2279 1165 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2330 2362 2644 2676 4663
Size: 32 135 32 758 32 282 32 1987
Cuts at: 4663 4695 4737 4769 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1987 758 282 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3366 3612 669
Size: 988 281 1428 246 3079
Fragments arranged by size:
3079 1428 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6022
HpaI GTT'AAC
Cuts at: 3612 3612
Size: 6022
KpnI G_GTAC'C
Cuts at: 3347 3347
Size: 6022
MfeI C'AATT_G
Cuts at: 3621 3621
Size: 6022
MluI A'CGCG_T
Cuts at: 5898 5898
Size: 6022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2301 2680 2847 5136 5320 1305
Size: 996 379 167 2289 184 2007
Fragments arranged by size:
2289 2007 996 379 184 167
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2828 10
Size: 54 1425 343 996 3204
Fragments arranged by size:
3204 1425 996 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3980 4339 4498 518
Size: 648 2814 359 159 2042
Fragments arranged by size:
2814 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2770 2953 3162 3438 4115 5263 5508 1606
Size: 1164 183 209 276 677 1148 245 2120
Fragments arranged by size:
2120 1164 1148 677 276 245 209 183
NcoI C'CATG_G
Cuts at: 513 1065 2526 513
Size: 552 1461 4009
Fragments arranged by size:
4009 1461 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6022
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6022
NotI GC'GGCC_GC
Cuts at: 3469 3469
Size: 6022
NspI r_CATG'y
Cuts at: 1132 1229 3380 4925 1132
Size: 97 2151 1545 2229
Fragments arranged by size:
2229 2151 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2690 1348
Size: 1342 4680
PciI A'CATG_T
Cuts at: 4921 4921
Size: 6022
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2596 1108
Size: 1488 4534
PfoI T'CCnGG_A
Cuts at: 2292 2484 2589 2808 3015 3276 2292
Size: 192 105 219 207 261 5038
Fragments arranged by size:
5038 261 219 207 192 105
PmeI GTTT'AAAC
Cuts at: 3326 3326
Size: 6022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2456 2488 4091 4123 5604 5636 2456
Size: 32 1603 32 1481 32 2842
Fragments arranged by size:
2842 1603 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6022
PsiI TTA'TAA
Cuts at: 3592 3592
Size: 6022
PspOMI G'GGCC_C
Cuts at: 2684 3183 2684
Size: 499 5523
PstI C_TGCA'G
Cuts at: 830 3374 830
Size: 2544 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6022
PvuII CAG'CTG
Cuts at: 2770 2953 2770
Size: 183 5839
SacI G_AGCT'C
Cuts at: 2202 2292 3249 2202
Size: 90 957 4975
Fragments arranged by size:
4975 957 90
SalI G'TCGA_C
Cuts at: 1936 3364 1936
Size: 1428 4594
SbfI CC_TGCA'GG
Cuts at: 3374 3374
Size: 6022
ScaI AGT'ACT
Cuts at: 4270 5982 4270
Size: 1712 4310
SexAI A'CCwGG_T
Cuts at: 2451 2451
Size: 6022
SfcI C'TryA_G
Cuts at: 826 1045 3370 4505 5186 5377 826
Size: 219 2325 1135 681 191 1471
Fragments arranged by size:
2325 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6022
SmaI CCC'GGG
Cuts at: 1872 2717 3089 3349 1872
Size: 845 372 260 4545
Fragments arranged by size:
4545 845 372 260
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2105 4126 5027 5289 820
Size: 197 926 162 2021 901 262 1553
Fragments arranged by size:
2021 1553 926 901 262 197 162
SnaBI TAC'GTA
Cuts at: 493 3813 493
Size: 3320 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6022
SphI G_CATG'C
Cuts at: 1132 3380 1132
Size: 2248 3774
SrfI GCCC'GGGC
Cuts at: 2717 3089 2717
Size: 372 5650
SspI AAT'ATT
Cuts at: 5 52 2023 2335 3946 5
Size: 47 1971 312 1611 2081
Fragments arranged by size:
2081 1971 1611 312 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6022
StyI C'CwwG_G
Cuts at: 513 1065 2526 2829 3461 513
Size: 552 1461 303 632 3074
Fragments arranged by size:
3074 1461 632 552 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2207 2447 2990 3266 4049 4066 4219
Size: 1255 627 240 543 276 783 17 153
Cuts at: 4219 4404 4849 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 783 627 543 445 276 240
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4268 5980 96
Size: 274 80 33 51 1443 2291 1712 138
Fragments arranged by size:
2291 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2439 2471 5982 6014 2439
Size: 32 3511 32 2447
Fragments arranged by size:
3511 2447 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6022
XbaI T'CTAG_A
Cuts at: 3358 3358
Size: 6022
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2321 2338 3004 2321
Size: 17 666 5339
Fragments arranged by size:
5339 666 17
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6022
XmaI C'CCGG_G
Cuts at: 1870 2715 3087 3347 1870
Size: 845 372 260 4545
Fragments arranged by size:
4545 845 372 260
XmnI GAAnn'nnTTC
Cuts at: 4151 4151
Size: 6022
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3830 276
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI
SwaI