Restriction map for
pFN21AE4423
(Circular) (Six-base) MAPSORT of: pFN21AE4423.seq Check: 9848 from: 1 to: 6019
pFN21AE4423 6019 bp
With 182 enzymes: SgfI *
March 28, 2012 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3829 278
Size: 53 83 186 936 2293 2468
Fragments arranged by size:
2468 2293 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2547 2991 3362 1937
Size: 610 444 371 4594
Fragments arranged by size:
4594 610 444 371
Acc65I G'GTAC_C
Cuts at: 3340 3340
Size: 6019
AclI AA'CG_TT
Cuts at: 1563 4146 4519 1563
Size: 2583 373 3063
Fragments arranged by size:
3063 2583 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4082 5466 5734 1632
Size: 2450 1384 268 1917
Fragments arranged by size:
2450 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5822
AflIII A'CryG_T
Cuts at: 1203 4918 5895 1203
Size: 3715 977 1327
Fragments arranged by size:
3715 1327 977
AgeI A'CCGG_T
Cuts at: 4850 4850
Size: 6019
AhdI GACnn_n'nnGTC
Cuts at: 4748 4748
Size: 6019
AleI CACnn'nnGTG
Cuts at: 1166 2664 2666 1166
Size: 1498 2 4519
Fragments arranged by size:
4519 1498 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6019
AlwNI CAG_nnn'CTG
Cuts at: 3762 5334 3762
Size: 1572 4447
ApaLI G'TGCA_C
Cuts at: 4076 5232 4076
Size: 1156 4863
ApoI r'AATT_y
Cuts at: 1493 2119 2321 3163 3328 3554 3706 5815 1493
Size: 626 202 842 165 226 152 2109 1697
Fragments arranged by size:
2109 1697 842 626 226 202 165 152
AseI AT'TA_AT
Cuts at: 160 1032 4573 160
Size: 872 3541 1606
Fragments arranged by size:
3541 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6019
AvaI C'yCGr_G
Cuts at: 1870 1943 3344 1870
Size: 73 1401 4545
Fragments arranged by size:
4545 1401 73
BamHI G'GATC_C
Cuts at: 2880 3349 3716 2880
Size: 469 367 5183
Fragments arranged by size:
5183 469 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3340 4795 618
Size: 325 217 627 1553 1455 1842
Fragments arranged by size:
1842 1553 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3338 1391
Size: 1947 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2678 2962 928
Size: 971 779 284 3985
Fragments arranged by size:
3985 971 779 284
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4210 4244 1903
Size: 34 50 34 2189 34 3678
Fragments arranged by size:
3678 2189 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3911 5121 3911
Size: 1210 4809
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6019
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4630 136
Size: 107 122 71 631 605 2958 1525
Fragments arranged by size:
2958 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6014 6014
Size: 6019
BlpI GC'TnA_GC
Cuts at: 3436 3436
Size: 6019
Bme1580I G_kGCm'C
Cuts at: 1790 2086 4080 5236 1790
Size: 296 1994 1156 2573
Fragments arranged by size:
2573 1994 1156 296
BmgBI CAC'GTC
Cuts at: 1318 3082 1318
Size: 1764 4255
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2168 2312 3750
Size: 416 53 83 399 24 751 144 1438
Cuts at: 3750 4708 442
Size: 958 1753
Fragments arranged by size:
1753 1438 958 751 416 399 144 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2012 1056
Size: 956 5063
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2590 4679 1239
Size: 1351 2089 2579
Fragments arranged by size:
2579 2089 1351
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2612 4144 5009 5307 2612
Size: 1532 865 298 3324
Fragments arranged by size:
3324 1532 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4682 882
Size: 617 3183 2219
Fragments arranged by size:
3183 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3810 493
Size: 711 2606 2702
Fragments arranged by size:
2702 2606 711
BsaBI GATnn'nnATC
Cuts at: 6013 6013
Size: 6019
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3826 4208 4843 275
Size: 53 83 186 936 2293 382 635 1451
Fragments arranged by size:
2293 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4452 4850 5124 5271 5682 4452
Size: 398 274 147 411 4789
Fragments arranged by size:
4789 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2355 2385 2880 2910 1178
Size: 30 165 30 952 30 495 30 4287
Fragments arranged by size:
4287 952 495 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2376 1186
Size: 1190 4829
BseYI C'CCAG_C
Cuts at: 2539 2841 3059 5222 5718 2539
Size: 302 218 2163 496 2840
Fragments arranged by size:
2840 2163 496 302 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2561 2724 1163
Size: 573 825 163 4458
Fragments arranged by size:
4458 825 573 163
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3469 4230 5258 1540
Size: 275 186 1468 761 1028 2301
Fragments arranged by size:
2301 1468 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4080 4165 5236 4080
Size: 85 1071 4863
Fragments arranged by size:
4863 1071 85
BsmI GAATG_Cn'
Cuts at: 3003 3530 3623 3003
Size: 527 93 5399
Fragments arranged by size:
5399 527 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 3338 4080 4165 5236 1391
Size: 399 296 1252 742 85 1071 2174
Fragments arranged by size:
2174 1252 1071 742 399 296 85
BspEI T'CCGG_A
Cuts at: 5682 5682
Size: 6019
BspHI T'CATG_A
Cuts at: 3908 5638 3908
Size: 1730 4289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3374 844
Size: 2530 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3906 1394
Size: 429 2083 3507
Fragments arranged by size:
3507 2083 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4514 4688 65
Size: 1183 3266 174 1396
Fragments arranged by size:
3266 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4663 4850 1700
Size: 110 2853 187 2869
Fragments arranged by size:
2869 2853 187 110
BsrGI T'GTAC_A
Cuts at: 96 3004 3272 96
Size: 2908 268 2843
Fragments arranged by size:
2908 2843 268
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6019
BssSI C'ACGA_G
Cuts at: 2013 4079 5091 2013
Size: 2066 1012 2941
Fragments arranged by size:
2941 2066 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2852 794
Size: 2058 3961
BstBI TT'CG_AA
Cuts at: 4823 4823
Size: 6019
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6019
BstXI CCAn_nnnn'nTGG
Cuts at: 2253 2403 2253
Size: 150 5869
BstYI r'GATC_y
Cuts at: 1972 2306 2880 3349 3716 4101 4118 5570 5656
Size: 334 574 469 367 385 17 1452 86
Cuts at: 5656 5668 6014 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 574 469 385 367 346 334
86 17 12
BstZ17I GTA'TAC
Cuts at: 2992 2992
Size: 6019
BtgI C'CryG_G
Cuts at: 513 1065 2876 513
Size: 552 1811 3656
Fragments arranged by size:
3656 1811 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2299 487
Size: 683 56 1073 4207
Fragments arranged by size:
4207 1073 683 56
BtsI GCAGTG_nn'
Cuts at: 2084 3535 4329 4349 2084
Size: 1451 794 20 3754
Fragments arranged by size:
3754 1451 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6019
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5984
DraI TTT'AAA
Cuts at: 3034 3323 3034
Size: 289 5730
DraIII CAC_nnn'GTG
Cuts at: 3052 3052
Size: 6019
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5026 809
Size: 507 3710 1802
Fragments arranged by size:
3710 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2694 3466 4355
Size: 54 1425 50 275 18 864 772 889
Cuts at: 4355 5984 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 864 772 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3466 1537
Size: 275 1654 4090
Fragments arranged by size:
4090 1654 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2271 3949 1353
Size: 918 1678 3423
Fragments arranged by size:
3423 1678 918
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4606 4980 5126 387
Size: 1064 138 3017 374 146 1280
Fragments arranged by size:
3017 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2590 4082 4679 5466 5734 1239
Size: 393 958 1492 597 787 268 1524
Fragments arranged by size:
1524 1492 958 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2392 2392
Size: 6019
EcoRI G'AATT_C
Cuts at: 3328 3706 3328
Size: 378 5641
EcoRV GAT'ATC
Cuts at: 2726 3118 2726
Size: 392 5627
FspI TGC'GCA
Cuts at: 4525 4525
Size: 6019
HaeII r_GCGC'y
Cuts at: 1678 2059 5166 1678
Size: 381 3107 2531
Fragments arranged by size:
3107 2531 381
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2020 2052 4660 4692 4734
Size: 32 135 32 448 32 2608 32 42
Cuts at: 4734 4766 1373
Size: 32 2626
Fragments arranged by size:
2626 2608 448 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3363 3609 669
Size: 988 281 1425 246 3079
Fragments arranged by size:
3079 1425 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3037 748
Size: 2289 3730
HpaI GTT'AAC
Cuts at: 3609 3609
Size: 6019
KpnI G_GTAC'C
Cuts at: 3344 3344
Size: 6019
MfeI C'AATT_G
Cuts at: 3618 3618
Size: 6019
MluI A'CGCG_T
Cuts at: 5895 5895
Size: 6019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2151 5133 5317 1305
Size: 846 2982 184 2007
Fragments arranged by size:
2982 2007 846 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2696 10
Size: 54 1425 343 864 3333
Fragments arranged by size:
3333 1425 864 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2563 2649 2664 2666 3977 4336 4495
Size: 648 1397 86 15 2 1311 359 159
Cuts at: 4495 518
Size: 2042
Fragments arranged by size:
2042 1397 1311 648 359 159 86 15
2
MspA1I CmG'CkG
Cuts at: 1606 2464 3435 4112 5260 5505 1606
Size: 858 971 677 1148 245 2120
Fragments arranged by size:
2120 1148 971 858 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5467
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6019
NheI G'CTAG_C
Cuts at: 1052 2008 1052
Size: 956 5063
NotI GC'GGCC_GC
Cuts at: 3466 3466
Size: 6019
NspI r_CATG'y
Cuts at: 1132 1229 2648 2895 3377 4922 1132
Size: 97 1419 247 482 1545 2229
Fragments arranged by size:
2229 1545 1419 482 247 97
PasI CC'CwG_GG
Cuts at: 1348 2388 1348
Size: 1040 4979
PciI A'CATG_T
Cuts at: 4918 4918
Size: 6019
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6019
PfoI T'CCnGG_A
Cuts at: 2196 2449 3245 2196
Size: 253 796 4970
Fragments arranged by size:
4970 796 253
PmeI GTTT'AAAC
Cuts at: 3323 3323
Size: 6019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4088 4120 5601 5633 4088
Size: 32 1481 32 4474
Fragments arranged by size:
4474 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2392 2392
Size: 6019
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6019
PsiI TTA'TAA
Cuts at: 3589 3589
Size: 6019
PstI C_TGCA'G
Cuts at: 830 3371 830
Size: 2541 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6019
PvuII CAG'CTG
Cuts at: 2464 2464
Size: 6019
SalI G'TCGA_C
Cuts at: 1936 3361 1936
Size: 1425 4594
SanDI GG'GwC_CC
Cuts at: 2392 2392
Size: 6019
SbfI CC_TGCA'GG
Cuts at: 3371 3371
Size: 6019
ScaI AGT'ACT
Cuts at: 4267 5979 4267
Size: 1712 4307
SfcI C'TryA_G
Cuts at: 826 1045 2485 3367 4502 5183 5374 826
Size: 219 1440 882 1135 681 191 1471
Fragments arranged by size:
1471 1440 1135 882 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6019
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6019
SmaI CCC'GGG
Cuts at: 1872 3346 1872
Size: 1474 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2627 4123 5024 5286 820
Size: 197 926 684 1496 901 262 1553
Fragments arranged by size:
1553 1496 926 901 684 262 197
SnaBI TAC'GTA
Cuts at: 493 3810 493
Size: 3317 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6019
SphI G_CATG'C
Cuts at: 1132 3377 1132
Size: 2245 3774
SspI AAT'ATT
Cuts at: 5 52 3943 5
Size: 47 3891 2081
Fragments arranged by size:
3891 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6019
StyI C'CwwG_G
Cuts at: 513 1065 3458 513
Size: 552 2393 3074
Fragments arranged by size:
3074 2393 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2762 4046 4063 4216 4401 4846 325
Size: 1255 1182 1284 17 153 185 445 1498
Fragments arranged by size:
1498 1284 1255 1182 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3004 3272 4265
Size: 274 80 33 51 1443 1027 268 993
Cuts at: 4265 5977 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1027 993 274 268 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5979 6011 5979
Size: 32 5987
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6019
XbaI T'CTAG_A
Cuts at: 3355 3355
Size: 6019
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2253 2756 2253
Size: 503 5516
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6019
XmaI C'CCGG_G
Cuts at: 1870 3344 1870
Size: 1474 4545
XmnI GAAnn'nnTTC
Cuts at: 3205 4148 3205
Size: 943 5076
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3827 276
Size: 53 83 186 936 2293 2468
Fragments arranged by size:
2468 2293 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI