Restriction map for
pFN21AE4422
(Circular) (Six-base) MAPSORT of: pFN21AE4422.seq Check: 6375 from: 1 to: 6016
pFN21AE4422 6016 bp
With 182 enzymes: SgfI *
March 28, 2012 13:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2156 2156
Size: 6016
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3826 278
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2154 3359 1937
Size: 217 1205 4594
Fragments arranged by size:
4594 1205 217
Acc65I G'GTAC_C
Cuts at: 3337 3337
Size: 6016
AclI AA'CG_TT
Cuts at: 1563 4143 4516 1563
Size: 2580 373 3063
Fragments arranged by size:
3063 2580 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2678 4079 5463 5731 1632
Size: 1046 1401 1384 268 1917
Fragments arranged by size:
1917 1401 1384 1046 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5819
AflIII A'CryG_T
Cuts at: 1203 2774 3123 4915 5892 1203
Size: 1571 349 1792 977 1327
Fragments arranged by size:
1792 1571 1327 977 349
AgeI A'CCGG_T
Cuts at: 4847 4847
Size: 6016
AhdI GACnn_n'nnGTC
Cuts at: 4745 4745
Size: 6016
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6016
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2120 3251 1184
Size: 936 1131 3949
Fragments arranged by size:
3949 1131 936
AlwNI CAG_nnn'CTG
Cuts at: 3759 5331 3759
Size: 1572 4444
ApaLI G'TGCA_C
Cuts at: 4073 5229 4073
Size: 1156 4860
ApoI r'AATT_y
Cuts at: 1493 2213 2272 2492 2786 2820 3325 3551 3703
Size: 720 59 220 294 34 505 226 152
Cuts at: 3703 5812 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 720 505 294 226 220 152
59 34
AseI AT'TA_AT
Cuts at: 160 1032 2448 2649 4570 160
Size: 872 1416 201 1921 1606
Fragments arranged by size:
1921 1606 1416 872 201
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
AvaI C'yCGr_G
Cuts at: 1870 1943 3341 1870
Size: 73 1398 4545
Fragments arranged by size:
4545 1398 73
BamHI G'GATC_C
Cuts at: 3346 3713 3346
Size: 367 5649
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2635 3337 4792 618
Size: 325 217 627 848 702 1455 1842
Fragments arranged by size:
1842 1455 848 702 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2084 3335 1391
Size: 693 1251 4072
Fragments arranged by size:
4072 1251 693
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2472 928
Size: 971 573 4472
Fragments arranged by size:
4472 971 573
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4207 4241 1903
Size: 34 50 34 2186 34 3678
Fragments arranged by size:
3678 2186 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3321 3908 5118 3321
Size: 587 1210 4219
Fragments arranged by size:
4219 1210 587
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6016
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4627 136
Size: 107 122 71 631 605 2955 1525
Fragments arranged by size:
2955 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6011 6011
Size: 6016
BlpI GC'TnA_GC
Cuts at: 3433 3433
Size: 6016
Bme1580I G_kGCm'C
Cuts at: 1790 2976 4077 5233 1790
Size: 1186 1101 1156 2573
Fragments arranged by size:
2573 1186 1156 1101
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6016
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3195 3747 4705
Size: 416 53 83 399 24 1778 552 958
Cuts at: 4705 442
Size: 1753
Fragments arranged by size:
1778 1753 958 552 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4676 1239
Size: 3437 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4141 5006 5304 4141
Size: 865 298 4853
Fragments arranged by size:
4853 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2028 4679 882
Size: 617 529 2651 2219
Fragments arranged by size:
2651 2219 617 529
BsaAI yAC'GTr
Cuts at: 493 1204 3807 493
Size: 711 2603 2702
Fragments arranged by size:
2702 2603 711
BsaBI GATnn'nnATC
Cuts at: 6010 6010
Size: 6016
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3823 4205 4840 275
Size: 53 83 186 936 2290 382 635 1451
Fragments arranged by size:
2290 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4449 4847 5121 5268 5679 4449
Size: 398 274 147 411 4786
Fragments arranged by size:
4786 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5791
Fragments arranged by size:
5791 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6016
BseYI C'CCAG_C
Cuts at: 2029 5219 5715 2029
Size: 3190 496 2330
Fragments arranged by size:
3190 2330 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2706 1163
Size: 573 970 4473
Fragments arranged by size:
4473 970 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2146 3466 4227 5255 1540
Size: 275 186 145 1320 761 1028 2301
Fragments arranged by size:
2301 1320 1028 761 275 186 145
BsiHKAI G_wGCw'C
Cuts at: 4077 4162 5233 4077
Size: 85 1071 4860
Fragments arranged by size:
4860 1071 85
BsmI GAATG_Cn'
Cuts at: 3527 3620 3527
Size: 93 5923
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2976 3335 4077 4162 5233 1391
Size: 399 294 892 359 742 85 1071 2174
Fragments arranged by size:
2174 1071 892 742 399 359 294 85
BspEI T'CCGG_A
Cuts at: 5679 5679
Size: 6016
BspHI T'CATG_A
Cuts at: 3905 5635 3905
Size: 1730 4286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2156 3371 844
Size: 1312 1215 3489
Fragments arranged by size:
3489 1312 1215
BsrBI CCG'CTC
Cuts at: 1394 1823 3903 1394
Size: 429 2080 3507
Fragments arranged by size:
3507 2080 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4511 4685 65
Size: 1183 3263 174 1396
Fragments arranged by size:
3263 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4660 4847 1700
Size: 110 2850 187 2869
Fragments arranged by size:
2869 2850 187 110
BsrGI T'GTAC_A
Cuts at: 96 2115 96
Size: 2019 3997
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6016
BssSI C'ACGA_G
Cuts at: 4076 5088 4076
Size: 1012 5004
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3262 794
Size: 2468 3548
BstBI TT'CG_AA
Cuts at: 4820 4820
Size: 6016
BstEII G'GTnAC_C
Cuts at: 1184 2282 1184
Size: 1098 4918
BstYI r'GATC_y
Cuts at: 1972 3346 3713 4098 4115 5567 5653 5665 6011
Size: 1374 367 385 17 1452 86 12 346
Cuts at: 6011 1972
Size: 1977
Fragments arranged by size:
1977 1452 1374 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2155 2155
Size: 6016
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5464
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5277
Fragments arranged by size:
5277 683 56
BtsI GCAGTG_nn'
Cuts at: 2568 2988 3532 4326 4346 2568
Size: 420 544 794 20 4238
Fragments arranged by size:
4238 794 544 420 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6016
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5981
DraI TTT'AAA
Cuts at: 3320 3320
Size: 6016
DraIII CAC_nnn'GTG
Cuts at: 3181 3181
Size: 6016
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5023 809
Size: 507 3707 1802
Fragments arranged by size:
3707 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2529 3463 4352
Size: 54 1425 50 275 18 699 934 889
Cuts at: 4352 5981 8
Size: 1629 43
Fragments arranged by size:
1629 1425 934 889 699 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3463 1537
Size: 275 1651 4090
Fragments arranged by size:
4090 1651 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3946 1353
Size: 2593 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4603 4977 5123 387
Size: 1064 138 3014 374 146 1280
Fragments arranged by size:
3014 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2678 4079 4676 5463 5731 1239
Size: 393 1046 1401 597 787 268 1524
Fragments arranged by size:
1524 1401 1046 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2407 2660 2407
Size: 253 5763
EcoO109I rG'GnC_Cy
Cuts at: 2352 2352
Size: 6016
EcoRI G'AATT_C
Cuts at: 2492 3325 3703 2492
Size: 833 378 4805
Fragments arranged by size:
4805 833 378
FspI TGC'GCA
Cuts at: 4522 4522
Size: 6016
HaeII r_GCGC'y
Cuts at: 1678 2590 5163 1678
Size: 912 2573 2531
Fragments arranged by size:
2573 2531 912
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2734 2766 4657 4689 4731
Size: 32 135 32 1162 32 1891 32 42
Cuts at: 4731 4763 1373
Size: 32 2626
Fragments arranged by size:
2626 1891 1162 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3159 3360 3606 669
Size: 988 281 1221 201 246 3079
Fragments arranged by size:
3079 1221 988 281 246 201
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6016
HpaI GTT'AAC
Cuts at: 3606 3606
Size: 6016
KpnI G_GTAC'C
Cuts at: 3341 3341
Size: 6016
MfeI C'AATT_G
Cuts at: 2009 2738 3615 2009
Size: 729 877 4410
Fragments arranged by size:
4410 877 729
MluI A'CGCG_T
Cuts at: 5892 5892
Size: 6016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3097 5130 5314 1305
Size: 1792 2033 184 2007
Fragments arranged by size:
2033 2007 1792 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2531 10
Size: 54 1425 343 699 3495
Fragments arranged by size:
3495 1425 699 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3087 3233 3974 4333 4492 518
Size: 648 1921 146 741 359 159 2042
Fragments arranged by size:
2042 1921 741 648 359 159 146
MspA1I CmG'CkG
Cuts at: 1606 2363 3432 4109 5257 5502 1606
Size: 757 1069 677 1148 245 2120
Fragments arranged by size:
2120 1148 1069 757 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5464
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6016
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6016
NotI GC'GGCC_GC
Cuts at: 3463 3463
Size: 6016
NspI r_CATG'y
Cuts at: 1132 1229 2778 3058 3127 3238 3374 4919 1132
Size: 97 1549 280 69 111 136 1545 2229
Fragments arranged by size:
2229 1549 1545 280 136 111 97 69
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6016
PciI A'CATG_T
Cuts at: 2774 3123 4915 2774
Size: 349 1792 3875
Fragments arranged by size:
3875 1792 349
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2234 1108
Size: 1126 4890
PmeI GTTT'AAAC
Cuts at: 3320 3320
Size: 6016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4085 4117 5598 5630 4085
Size: 32 1481 32 4471
Fragments arranged by size:
4471 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2352 2352
Size: 6016
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6016
PsiI TTA'TAA
Cuts at: 3586 3586
Size: 6016
PstI C_TGCA'G
Cuts at: 830 2464 3368 830
Size: 1634 904 3478
Fragments arranged by size:
3478 1634 904
PvuI CG_AT'CG
Cuts at: 2001 2146 2001
Size: 145 5871
PvuII CAG'CTG
Cuts at: 2363 2363
Size: 6016
SalI G'TCGA_C
Cuts at: 1936 3358 1936
Size: 1422 4594
SanDI GG'GwC_CC
Cuts at: 2352 2352
Size: 6016
SbfI CC_TGCA'GG
Cuts at: 3368 3368
Size: 6016
ScaI AGT'ACT
Cuts at: 4264 5976 4264
Size: 1712 4304
SfcI C'TryA_G
Cuts at: 826 1045 2460 3297 3364 4499 5180 5371 826
Size: 219 1415 837 67 1135 681 191 1471
Fragments arranged by size:
1471 1415 1135 837 681 219 191 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6016
SmaI CCC'GGG
Cuts at: 1872 3343 1872
Size: 1471 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4120 5021 5283 820
Size: 197 926 2177 901 262 1553
Fragments arranged by size:
2177 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3807 493
Size: 3314 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6016
SphI G_CATG'C
Cuts at: 1132 3374 1132
Size: 2242 3774
SspI AAT'ATT
Cuts at: 5 52 2201 3940 5
Size: 47 2149 1739 2081
Fragments arranged by size:
2149 2081 1739 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6016
StyI C'CwwG_G
Cuts at: 513 1065 2130 3108 3455 513
Size: 552 1065 978 347 3074
Fragments arranged by size:
3074 1065 978 552 347
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2307 4043 4060 4213 4398 4843 325
Size: 1255 727 1736 17 153 185 445 1498
Fragments arranged by size:
1736 1498 1255 727 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2115 4262 5974
Size: 274 80 33 51 1443 138 2147 1712
Cuts at: 5974 96
Size: 138
Fragments arranged by size:
2147 1712 1443 274 138 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3054 3086 5976 6008 3054
Size: 32 2890 32 3062
Fragments arranged by size:
3062 2890 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2058 1316
Size: 742 5274
XbaI T'CTAG_A
Cuts at: 3352 3352
Size: 6016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3182 3182
Size: 6016
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6016
XmaI C'CCGG_G
Cuts at: 1870 3341 1870
Size: 1471 4545
XmnI GAAnn'nnTTC
Cuts at: 2868 4145 2868
Size: 1277 4739
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3824 276
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI