(Circular) (Six-base) MAPSORT of: pFN21AE4421.seq Check: 8421 from: 1 to: 6016 pFN21AE4421 6016 bp With 182 enzymes: SgfI * March 28, 2012 14:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3826 278 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2675 3359 1937 Size: 738 684 4594 Fragments arranged by size: 4594 738 684 Acc65I G'GTAC_C Cuts at: 3337 3337 Size: 6016 AclI AA'CG_TT Cuts at: 1563 4143 4516 1563 Size: 2580 373 3063 Fragments arranged by size: 3063 2580 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2153 4079 5463 5731 1632 Size: 521 1926 1384 268 1917 Fragments arranged by size: 1926 1917 1384 521 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5819 AflIII A'CryG_T Cuts at: 1203 4915 5892 1203 Size: 3712 977 1327 Fragments arranged by size: 3712 1327 977 AgeI A'CCGG_T Cuts at: 4847 4847 Size: 6016 AhdI GACnn_n'nnGTC Cuts at: 2089 4745 2089 Size: 2656 3360 AleI CACnn'nnGTG Cuts at: 1166 2180 2411 2691 1166 Size: 1014 231 280 4491 Fragments arranged by size: 4491 1014 280 231 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6016 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2559 2591 2559 Size: 32 5984 AlwNI CAG_nnn'CTG Cuts at: 2441 3759 5331 2441 Size: 1318 1572 3126 Fragments arranged by size: 3126 1572 1318 ApaLI G'TGCA_C Cuts at: 4073 5229 4073 Size: 1156 4860 ApoI r'AATT_y Cuts at: 1493 2786 2823 3325 3551 3703 5812 1493 Size: 1293 37 502 226 152 2109 1697 Fragments arranged by size: 2109 1697 1293 502 226 152 37 AseI AT'TA_AT Cuts at: 160 1032 4570 160 Size: 872 3538 1606 Fragments arranged by size: 3538 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 AvaI C'yCGr_G Cuts at: 1870 1943 2046 3341 1870 Size: 73 103 1295 4545 Fragments arranged by size: 4545 1295 103 73 BamHI G'GATC_C Cuts at: 2666 3346 3713 2666 Size: 680 367 4969 Fragments arranged by size: 4969 680 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3337 4792 618 Size: 325 217 627 1550 1455 1842 Fragments arranged by size: 1842 1550 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2075 3335 1391 Size: 684 1260 4072 Fragments arranged by size: 4072 1260 684 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2174 2462 928 Size: 971 275 288 4482 Fragments arranged by size: 4482 971 288 275 BbvCI CC'TCA_GC Cuts at: 2095 3225 2095 Size: 1130 4886 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4207 4241 1903 Size: 34 2270 34 3678 Fragments arranged by size: 3678 2270 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2133 3908 5118 2133 Size: 1775 1210 3031 Fragments arranged by size: 3031 1775 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6016 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4627 136 Size: 107 122 71 631 605 2955 1525 Fragments arranged by size: 2955 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6011 6011 Size: 6016 BlpI GC'TnA_GC Cuts at: 2730 2913 3433 2730 Size: 183 520 5313 Fragments arranged by size: 5313 520 183 Bme1580I G_kGCm'C Cuts at: 1790 4077 5233 1790 Size: 2287 1156 2573 Fragments arranged by size: 2573 2287 1156 BmgBI CAC'GTC Cuts at: 1318 3166 1318 Size: 1848 4168 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2352 2385 2672 Size: 416 53 83 399 24 935 33 287 Cuts at: 2672 3747 4705 442 Size: 1075 958 1753 Fragments arranged by size: 1753 1075 958 935 416 399 287 83 53 33 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6016 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2951 4676 1239 Size: 1712 1725 2579 Fragments arranged by size: 2579 1725 1712 Bpu10I CC'TnA_GC Cuts at: 2095 2835 3225 3249 2095 Size: 740 390 24 4862 Fragments arranged by size: 4862 740 390 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4141 5006 5304 4141 Size: 865 298 4853 Fragments arranged by size: 4853 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3281 4679 882 Size: 617 1782 1398 2219 Fragments arranged by size: 2219 1782 1398 617 BsaAI yAC'GTr Cuts at: 493 1204 2079 2930 2976 3807 493 Size: 711 875 851 46 831 2702 Fragments arranged by size: 2702 875 851 831 711 46 BsaBI GATnn'nnATC Cuts at: 6010 6010 Size: 6016 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3823 4205 4840 275 Size: 53 83 186 936 2290 382 635 1451 Fragments arranged by size: 2290 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4449 4847 5121 5268 5679 4449 Size: 398 274 147 411 4786 Fragments arranged by size: 4786 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2559 2589 1178 Size: 30 165 30 1156 30 4605 Fragments arranged by size: 4605 1156 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2291 3104 1186 Size: 1105 813 4098 Fragments arranged by size: 4098 1105 813 BseYI C'CCAG_C Cuts at: 3291 5219 5715 3291 Size: 1928 496 3592 Fragments arranged by size: 3592 1928 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3282 1163 Size: 573 1546 3897 Fragments arranged by size: 3897 1546 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3466 4227 5255 1540 Size: 275 186 1465 761 1028 2301 Fragments arranged by size: 2301 1465 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2075 2200 2919 4077 4162 5233 2075 Size: 125 719 1158 85 1071 2858 Fragments arranged by size: 2858 1158 1071 719 125 85 BsmI GAATG_Cn' Cuts at: 3250 3527 3620 3250 Size: 277 93 5646 Fragments arranged by size: 5646 277 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 2200 2919 3335 4077 4162 5233 Size: 399 285 125 719 416 742 85 1071 Cuts at: 5233 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 719 416 399 285 125 85 BspEI T'CCGG_A Cuts at: 5679 5679 Size: 6016 BspHI T'CATG_A Cuts at: 3905 5635 3905 Size: 1730 4286 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2067 3371 844 Size: 1223 1304 3489 Fragments arranged by size: 3489 1304 1223 BsrBI CCG'CTC Cuts at: 1394 1823 3903 1394 Size: 429 2080 3507 Fragments arranged by size: 3507 2080 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3085 4511 4685 65 Size: 1183 1837 1426 174 1396 Fragments arranged by size: 1837 1426 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4660 4847 1700 Size: 110 2850 187 2869 Fragments arranged by size: 2869 2850 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6016 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6016 BssSI C'ACGA_G Cuts at: 2516 4076 5088 2516 Size: 1560 1012 3444 Fragments arranged by size: 3444 1560 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2015 2354 794 Size: 1221 339 4456 Fragments arranged by size: 4456 1221 339 BstBI TT'CG_AA Cuts at: 4820 4820 Size: 6016 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6016 BstXI CCAn_nnnn'nTGG Cuts at: 2467 3040 2467 Size: 573 5443 BstYI r'GATC_y Cuts at: 1972 2666 3346 3713 4098 4115 5567 5653 5665 Size: 694 680 367 385 17 1452 86 12 Cuts at: 5665 6011 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 694 680 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2181 513 Size: 552 939 177 4348 Fragments arranged by size: 4348 939 552 177 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5277 Fragments arranged by size: 5277 683 56 BtsI GCAGTG_nn' Cuts at: 2442 2452 2530 3532 4326 4346 2442 Size: 10 78 1002 794 20 4112 Fragments arranged by size: 4112 1002 794 78 20 10 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6016 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5981 DraI TTT'AAA Cuts at: 3094 3320 3094 Size: 226 5790 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5023 809 Size: 507 3707 1802 Fragments arranged by size: 3707 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2184 2394 3463 Size: 54 1425 50 275 18 354 210 1069 Cuts at: 3463 4352 5981 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1069 889 354 275 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3463 1537 Size: 275 1651 4090 Fragments arranged by size: 4090 1651 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3946 1353 Size: 2593 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4603 4977 5123 387 Size: 1064 138 3014 374 146 1280 Fragments arranged by size: 3014 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2073 2073 Size: 6016 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2951 4079 4676 5463 5731 1239 Size: 393 521 798 1128 597 787 268 1524 Fragments arranged by size: 1524 1128 798 787 597 521 393 268 EcoO109I rG'GnC_Cy Cuts at: 3212 3212 Size: 6016 EcoRI G'AATT_C Cuts at: 3325 3703 3325 Size: 378 5638 FspI TGC'GCA Cuts at: 4522 4522 Size: 6016 HaeII r_GCGC'y Cuts at: 1678 2337 5163 1678 Size: 659 2826 2531 Fragments arranged by size: 2826 2531 659 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2075 2107 2182 2214 2740 Size: 32 135 32 503 32 75 32 526 Cuts at: 2740 2772 3050 3082 3160 3192 3223 3255 4657 Size: 32 278 32 78 32 31 32 1402 Cuts at: 4657 4689 4731 4763 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1402 526 503 278 135 78 75 42 32 32 32 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 2056 3184 3360 3606 669 Size: 988 281 118 1128 176 246 3079 Fragments arranged by size: 3079 1128 988 281 246 176 118 HindIII A'AGCT_T Cuts at: 748 2681 748 Size: 1933 4083 HpaI GTT'AAC Cuts at: 3184 3606 3184 Size: 422 5594 KpnI G_GTAC'C Cuts at: 3341 3341 Size: 6016 MfeI C'AATT_G Cuts at: 2940 3615 2940 Size: 675 5341 MluI A'CGCG_T Cuts at: 5892 5892 Size: 6016 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2483 3018 5130 5314 1305 Size: 1178 535 2112 184 2007 Fragments arranged by size: 2112 2007 1178 535 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2186 2396 10 Size: 54 1425 343 354 210 3630 Fragments arranged by size: 3630 1425 354 343 210 54 MslI CAynn'nnrTG Cuts at: 518 1166 2180 2411 2691 3044 3974 4333 4492 Size: 648 1014 231 280 353 930 359 159 Cuts at: 4492 518 Size: 2042 Fragments arranged by size: 2042 1014 930 648 359 353 280 231 159 MspA1I CmG'CkG Cuts at: 1606 3136 3229 3432 4109 5257 5502 1606 Size: 1530 93 203 677 1148 245 2120 Fragments arranged by size: 2120 1530 1148 677 245 203 93 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4525 Fragments arranged by size: 4525 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6016 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6016 NotI GC'GGCC_GC Cuts at: 3463 3463 Size: 6016 NspI r_CATG'y Cuts at: 1132 1229 3374 4919 1132 Size: 97 2145 1545 2229 Fragments arranged by size: 2229 2145 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6016 PciI A'CATG_T Cuts at: 4915 4915 Size: 6016 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6016 PmeI GTTT'AAAC Cuts at: 3320 3320 Size: 6016 PmlI CAC'GTG Cuts at: 2976 2976 Size: 6016 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2559 2591 4085 4117 5598 5630 2559 Size: 32 1494 32 1481 32 2945 Fragments arranged by size: 2945 1494 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3212 3212 Size: 6016 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6016 PsiI TTA'TAA Cuts at: 3586 3586 Size: 6016 PstI C_TGCA'G Cuts at: 830 3141 3368 830 Size: 2311 227 3478 Fragments arranged by size: 3478 2311 227 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6016 PvuII CAG'CTG Cuts at: 3136 3229 3136 Size: 93 5923 SacI G_AGCT'C Cuts at: 2075 2075 Size: 6016 SalI G'TCGA_C Cuts at: 1936 3358 1936 Size: 1422 4594 SbfI CC_TGCA'GG Cuts at: 3368 3368 Size: 6016 ScaI AGT'ACT Cuts at: 4264 5976 4264 Size: 1712 4304 SfcI C'TryA_G Cuts at: 826 1045 3137 3364 4499 5180 5371 826 Size: 219 2092 227 1135 681 191 1471 Fragments arranged by size: 2092 1471 1135 681 227 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6016 SmaI CCC'GGG Cuts at: 1872 3343 1872 Size: 1471 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4120 5021 5283 820 Size: 197 926 2177 901 262 1553 Fragments arranged by size: 2177 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3807 493 Size: 3314 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6016 SphI G_CATG'C Cuts at: 1132 3374 1132 Size: 2242 3774 SspI AAT'ATT Cuts at: 5 52 3940 5 Size: 47 3888 2081 Fragments arranged by size: 3888 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6016 StyI C'CwwG_G Cuts at: 513 1065 2004 2064 2382 3001 3256 3455 513 Size: 552 939 60 318 619 255 199 3074 Fragments arranged by size: 3074 939 619 552 318 255 199 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4043 4060 4213 4398 4843 325 Size: 1255 2463 17 153 185 445 1498 Fragments arranged by size: 2463 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2108 4262 5974 Size: 274 80 33 51 1443 131 2154 1712 Cuts at: 5974 96 Size: 138 Fragments arranged by size: 2154 1712 1443 274 138 131 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3003 3035 5976 6008 3003 Size: 32 2941 32 3011 Fragments arranged by size: 3011 2941 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2461 3235 1316 Size: 1145 774 4097 Fragments arranged by size: 4097 1145 774 XbaI T'CTAG_A Cuts at: 3352 3352 Size: 6016 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2179 2389 2179 Size: 210 5806 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6016 XmaI C'CCGG_G Cuts at: 1870 3341 1870 Size: 1471 4545 XmnI GAAnn'nnTTC Cuts at: 4145 4145 Size: 6016 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3824 276 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI